Strain identifier

BacDive ID: 1365

Type strain: Yes

Species: Lentibacillus salinarum

Strain Designation: AHS-1

Strain history: <- JH Yoon, KRIBB

NCBI tax ID(s): 446820 (species)

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General

@ref: 15637

BacDive-ID: 1365

DSM-Number: 21356

keywords: 16S sequence, Bacteria, facultative anaerobe, spore-forming, Gram-positive, motile, rod-shaped, colony-forming

description: Lentibacillus salinarum AHS-1 is a facultative anaerobe, spore-forming, Gram-positive bacterium that forms circular colonies and was isolated from Sediment samples from marine solar saltern.

NCBI tax id

  • NCBI tax id: 446820
  • Matching level: species

strain history

@refhistory
15637<- CCUG <- J.-H. Yoon; AHS-1
67771<- JH Yoon, KRIBB

doi: 10.13145/bacdive1365.20240510.9

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Caryophanales
  • family: Bacillaceae
  • genus: Lentibacillus
  • species: Lentibacillus salinarum
  • full scientific name: Lentibacillus salinarum Lee et al. 2008

@ref: 15637

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Bacillales

family: Bacillaceae

genus: Lentibacillus

species: Lentibacillus salinarum

full scientific name: Lentibacillus salinarum Lee et al. 2008

strain designation: AHS-1

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell widthcell shapemotilityflagellum arrangement
23002positive2.0-4.0 µm0.7-1.2 µmrod-shapedyesperitrichous
67771rod-shapedyesperitrichous
67771positive

colony morphology

@refcolony sizecolony colorcolony shapeincubation periodmedium used
230020.5-1.2 mmcream-yellowcircular4 daysMSG agar
603422 days

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
15637CASO AGAR (MERCK 105458) (DSMZ Medium 220)yeshttps://mediadive.dsmz.de/medium/220Name: CASO AGAR (Merck 105458) (DSMZ Medium 220; with strain-specific modifications) Composition: NaCl 100.0 g/l Agar 15.0 g/l Casein peptone 15.0 g/l Soy peptone 5.0 g/l Distilled water
23002MSG agaryes

culture temp

@refgrowthtypetemperature
15637positivegrowth35
23002positivegrowth15.0
23002positivegrowth45.0
23002nogrowth10.0
23002nogrowth46.0
23002positiveoptimum37.0-40.0
60342positivegrowth37-42
67771positivegrowth37-40

culture pH

@refabilitytypepH
23002positiveoptimum6.5-7.0
23002positivegrowth6.0
23002nogrowth5.5

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
23002facultative anaerobe
67771facultative anaerobe

spore formation

  • @ref: 23002
  • spore description: oval
  • spore formation: yes

halophily

@refsalttested relationconcentrationgrowthhalophily level
23002NaCloptimum10.0-12.0 %(w/v)
23002NaClgrowth24.0 %(w/v)positive
23002NaClgrowth>25.0 %(w/v)no
23002moderately halophilic

observation

  • @ref: 67771
  • observation: quinones: MK-7

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
2300217057cellobiose-builds acid from
2300215824D-fructose-builds acid from
2300216899D-mannitol-builds acid from
2300216024D-mannose-builds acid from
2300265327D-xylose-builds acid from
2300230849L-arabinose-builds acid from
2300217716lactose-builds acid from
2300217306maltose-builds acid from
2300217814salicin-builds acid from
2300230911sorbitol-builds acid from
2300217992sucrose-builds acid from
2300227082trehalose-builds acid from
23002casein-hydrolysis
2300217368hypoxanthine-hydrolysis
2300253426tween 80-hydrolysis
2300218186tyrosine-hydrolysis
2300215318xanthine-hydrolysis
2300212936D-galactose+builds acid from
2300217634D-glucose+builds acid from
2300216988D-ribose+builds acid from
230024853esculin+hydrolysis
2300217632nitrate+reduction

enzymes

@refvalueactivityec
23002catalase+1.11.1.6
23002cytochrome oxidase+1.9.3.1
23002urease-3.5.1.5

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentenrichment cultureenrichment culture compositionenrichment culture durationenrichment culture temperaturesampling date
23002Sediment samples from marine solar salternYellow Sea, ByunsanRepublic of KoreaKORAsiamodified S-G (MSG) mediumper litre of distilled water: 100 g NaCl, 20 g MgSO4.7H2O, 2 g KCl, 3 g trisodium citrate, 10 g yeast extract, 7.5 g Casamino acids (pH 7.2)30 days30.0
60342Soil sediment,marine solar salternByunsan,Yellow SeaRepublic of KoreaKORAsia2006-01-01
15637soil sediment, marine solar salternDaejeonRepublic of KoreaKORAsia
67771From marine solar saltern of the Yellow SeaByunsanRepublic of KoreaKORAsia

isolation source categories

Cat1Cat2Cat3
#Environmental#Aquatic#Marine
#Environmental#Aquatic#Sediment
#Condition#Saline

taxonmaps

  • @ref: 69479
  • File name: preview.99_63917.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_3;96_3088;97_11300;98_45217;99_63917&stattab=map
  • Last taxonomy: Lentibacillus salinarum subclade
  • 16S sequence: EF601571
  • Sequence Identity:
  • Total samples: 26
  • soil counts: 5
  • aquatic counts: 5
  • animal counts: 10
  • plant counts: 6

Safety information

risk assessment

  • @ref: 15637
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 15637
  • description: Lentibacillus salinarum strain AHS-1 16S ribosomal RNA gene, partial sequence
  • accession: EF601571
  • length: 1521
  • database: nuccore
  • NCBI tax ID: 446820

GC content

@refGC-contentmethod
1563749
2300249.0high performance liquid chromatography (HPLC)
6777149.0

External links

@ref: 15637

culture collection no.: DSM 21356, CCUG 54822, KCTC 13162

straininfo link

  • @ref: 71016
  • straininfo: 405432

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny18175680Lentibacillus salinarum sp. nov., isolated from a marine solar saltern in Korea.Lee SY, Choi WY, Oh TK, Yoon JHInt J Syst Evol Microbiol10.1099/ijs.0.65351-02008Bacillaceae/*classification/genetics/isolation & purification/physiology, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/analysis, Genes, rRNA, Geologic Sediments/*microbiology, Korea, Molecular Sequence Data, Nucleic Acid Hybridization, Phenotype, Phylogeny, RNA, Ribosomal, 16S/genetics, Seawater/*microbiology, Sequence Analysis, DNA, *Sodium Chloride, Species SpecificityGenetics
Phylogeny19671718Lentibacillus persicus sp. nov., a moderately halophilic species isolated from a saline lake.Sanchez-Porro C, Amoozegar MA, Fernandez AB, Babavalian Fard H, Ramezani M, Ventosa AInt J Syst Evol Microbiol10.1099/ijs.0.015396-02009Bacillaceae/classification/cytology/*genetics/isolation & purification, DNA Primers, DNA, Bacterial/chemistry/genetics, Molecular Sequence Data, Phylogeny, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Sodium Chloride, Water MicrobiologyGenetics
Phylogeny27002961Lentibacillus kimchii sp. nov., an extremely halophilic bacterium isolated from kimchi, a Korean fermented vegetable.Oh YJ, Lee HW, Lim SK, Kwon MS, Lee J, Jang JY, Lee JH, Park HW, Nam YD, Seo MJ, Roh SW, Choi HJAntonie Van Leeuwenhoek10.1007/s10482-016-0686-52016Bacillaceae/*classification/genetics/*isolation & purification/metabolism, Bacterial Typing Techniques, Cell Wall/metabolism, DNA, Bacterial/genetics, DNA, Ribosomal/genetics, Diaminopimelic Acid/metabolism, Fatty Acids/metabolism, Fermentation, Food Microbiology, Halobacteriales/classification/genetics/isolation & purification/metabolism, Peptidoglycan/chemistry, Phenotype, Phospholipids/metabolism, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Sodium Chloride/metabolism, Vegetables/*microbiology, Vitamin K 2/analogs & derivatives/metabolismMetabolism

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
15637Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 21356)https://www.dsmz.de/collection/catalogue/details/culture/DSM-21356
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
23002Soo-Young Lee,Woo-Young Choi,Tae-Kwang Oh,Jung-Hoon Yoon10.1099/ijs.0.65351-0Lentibacillus salinarum sp. nov., isolated from a marine solar saltern in KoreaIJSEM 58: 45-49 200818175680
60342Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 54822)https://www.ccug.se/strain?id=54822
67771Curators of the KCTChttps://kctc.kribb.re.kr/En/Kctc
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
71016Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID405432.1StrainInfo: A central database for resolving microbial strain identifiers