Strain identifier

BacDive ID: 136346

Type strain: No

Species: Lysinibacillus sphaericus

Strain Designation: 25

Strain history: CIP <- 1996, ATCC, Bacillus sphaericus <- W.W. Ford: strain 25

NCBI tax ID(s): 1421 (species)

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General

@ref: 35731

BacDive-ID: 136346

keywords: Bacteria, obligate aerobe, spore-forming, mesophilic, Gram-positive, motile, rod-shaped

description: Lysinibacillus sphaericus 25 is an obligate aerobe, spore-forming, mesophilic bacterium of the family Caryophanaceae.

NCBI tax id

  • NCBI tax id: 1421
  • Matching level: species

strain history

  • @ref: 35731
  • history: CIP <- 1996, ATCC, Bacillus sphaericus <- W.W. Ford: strain 25

doi: 10.13145/bacdive136346.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Caryophanales
  • family: Caryophanaceae
  • genus: Lysinibacillus
  • species: Lysinibacillus sphaericus
  • full scientific name: Lysinibacillus sphaericus (Meyer and Neide 1904) Ahmed et al. 2007

  • synonyms

    @refsynonym
    20215Lysinibacillus varians
    20215Bacillus sphaericus
    20215Lysinibacillus mangiferahumi
    20215Lysinibacillus mangiferihumi
    20215Lysinibacillus tabacifolii

@ref: 35731

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Caryophanales

family: Bacillaceae

genus: Lysinibacillus

species: Lysinibacillus sphaericus

strain designation: 25

type strain: no

Morphology

cell morphology

  • @ref: 35731
  • gram stain: positive
  • cell shape: rod-shaped
  • motility: yes

colony morphology

  • @ref: 35731
  • hemolysis ability: 1

Culture and growth conditions

culture medium

@refnamegrowthcompositionlink
35731MEDIUM 3 - Columbia agaryesColumbia agar (39.000 g);distilled water (1000.000 ml)
35731CIP Medium 3yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=3

culture temp

@refgrowthtypetemperaturerange
35731positivegrowth30mesophilic
35731positivegrowth22-45
35731nogrowth10psychrophilic
35731nogrowth55thermophilic

culture pH

  • @ref: 35731
  • ability: positive
  • type: growth
  • pH: 6

Physiology and metabolism

oxygen tolerance

  • @ref: 35731
  • oxygen tolerance: obligate aerobe

spore formation

  • @ref: 35731
  • spore formation: yes

halophily

@refsaltgrowthtested relationconcentration
35731NaClpositivegrowth0-6 %
35731NaClnogrowth8 %
35731NaClnogrowth10 %

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
68371Potassium 5-ketogluconate-builds acid from
68371Potassium 2-ketogluconate-builds acid from
68371gluconate-builds acid from24265
68371L-arabitol-builds acid from18403
68371D-arabitol-builds acid from18333
68371L-fucose-builds acid from18287
68371D-fucose-builds acid from28847
68371D-tagatose-builds acid from16443
68371D-lyxose-builds acid from62318
68371turanose-builds acid from32528
68371gentiobiose-builds acid from28066
68371xylitol-builds acid from17151
68371glycogen-builds acid from28087
68371starch-builds acid from28017
68371raffinose-builds acid from16634
68371melezitose-builds acid from6731
68371inulin-builds acid from15443
68371trehalose-builds acid from27082
68371sucrose-builds acid from17992
68371melibiose-builds acid from28053
68371lactose-builds acid from17716
68371maltose-builds acid from17306
68371cellobiose-builds acid from17057
68371salicin-builds acid from17814
68371esculin-builds acid from4853
68371arbutin-builds acid from18305
68371amygdalin-builds acid from27613
68371methyl alpha-D-glucopyranoside-builds acid from320061
68371methyl alpha-D-mannoside-builds acid from43943
68371D-sorbitol-builds acid from17924
68371D-mannitol-builds acid from16899
68371myo-inositol-builds acid from17268
68371galactitol-builds acid from16813
68371L-rhamnose-builds acid from62345
68371L-sorbose-builds acid from17266
68371D-mannose-builds acid from16024
68371D-fructose-builds acid from15824
68371D-glucose-builds acid from17634
68371D-galactose-builds acid from12936
68371methyl beta-D-xylopyranoside-builds acid from74863
68371ribitol-builds acid from15963
68371L-xylose-builds acid from65328
68371D-xylose-builds acid from65327
68371D-ribose-builds acid from16988
68371L-arabinose-builds acid from30849
68371D-arabinose-builds acid from17108
68371erythritol-builds acid from17113
68371glycerol-builds acid from17754
35731citrate-carbon source16947
35731esculin-hydrolysis4853
35731hippurate-hydrolysis606565
35731nitrate-reduction17632
35731nitrite-reduction16301
35731nitrate-respiration17632

metabolite production

  • @ref: 35731
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testmethylred-test
3573115688acetoin-
3573117234glucose-

enzymes

@refvalueactivityec
35731oxidase+
35731beta-galactosidase-3.2.1.23
35731alcohol dehydrogenase-1.1.1.1
35731gelatinase-
35731DNase+
35731caseinase-3.4.21.50
35731catalase+1.11.1.6
35731tween esterase-
35731gamma-glutamyltransferase-2.3.2.2
35731lecithinase-
35731lipase-
35731lysine decarboxylase-4.1.1.18
35731ornithine decarboxylase-4.1.1.17
35731protease-
35731urease-3.5.1.5
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase-3.4.11.1
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase-3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
35731-+++------++--------

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
35731---------------------+/----------------------------

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
35731-----------------------------------------------------------+---------------+--+-----+---+-+--------

Safety information

risk assessment

  • @ref: 35731
  • biosafety level: 1
  • biosafety level comment: Risk group (French classification)

External links

@ref: 35731

culture collection no.: CIP 104788, ATCC 4525, CCM 2177

straininfo link

  • @ref: 93517
  • straininfo: 46447

literature

Pubmed-IDtitleauthorsjournalDOIyearmeshtopictopic2
1413261Scanning force microscopy studies of the S-layers from Bacillus coagulans E38-66, Bacillus sphaericus CCM2177 and of an antibody binding process.Ohnesorge F, Heckl WM, Haberle W, Pum D, Sara M, Schindler H, Schilcher K, Kiener A, Smith DP, Sleytr UB, et al.Ultramicroscopy10.1016/0304-3991(92)90429-n1992Antibodies, Bacterial/*ultrastructure, Antigen-Antibody Reactions, Bacillus/immunology/*ultrastructure, Bacterial Outer Membrane Proteins/immunology/*ultrastructure, *Bacterial Proteins, *Membrane Glycoproteins, Microscopy/methods, Microscopy, Electron
9245752S-layer stabilized solid support lipid bilayers.Wetzer B, Pum D, Sleytr UBJ Struct Biol10.1006/jsbi.1997.38671997Bacillus, Bacterial Proteins/*chemistry, Crystallization, *Diglycerides/chemistry, Image Processing, Computer-Assisted, *Lipid Bilayers, Membrane Glycoproteins/*chemistry, Microscopy, Atomic Force
9373319Immunoreactivity of allergen (Bet v 1) conjugated to crystalline bacterial cell surface layers (S-layers).Jahn-Schmid B, Graninger M, Glozik M, Kupcu S, Ebner C, Unger FM, Sleytr UB, Messner PImmunotechnology10.1016/1380-2933(96)00041-31996Adjuvants, Immunologic, Allergens/chemistry/*immunology, Animals, Antigens, Plant, Bacillus/chemistry/*immunology, Bacterial Proteins/*immunology, Cell Wall/immunology, Crystallography, Cytokines/biosynthesis, Epitopes, Female, Gram-Positive Bacteria/chemistry/immunology, Humans, Hypersensitivity/therapy, Immunoglobulin E/immunology, Immunotherapy, Mice, Mice, Inbred BALB C, Microscopy, Electron, Plant Proteins/*immunology, Pollen/*immunology, T-Lymphocytes/immunology, Th2 Cells/immunology, Trees/immunology, Vaccines, Synthetic/chemistryPathogenicity
9876158Bacterial S-layer protein coupling to lipids: x-ray reflectivity and grazing incidence diffraction studies.Weygand M, Wetzer B, Pum D, Sleytr UB, Cuvillier N, Kjaer K, Howes PB, Losche MBiophys J10.1016/S0006-3495(99)77213-91999Amino Acids/analysis, Bacillus/chemistry, Bacterial Outer Membrane Proteins/*chemistry, Biophysical Phenomena, Biophysics, Crystallography, X-Ray, Membrane Lipids/*chemistry, Phosphatidylethanolamines/chemistry, Spectrum Analysis, Static Electricity, X-RaysLipids
10601228Structural and functional analyses of the secondary cell wall polymer of Bacillus sphaericus CCM 2177 that serves as an S-layer-specific anchor.Ilk N, Kosma P, Puchberger M, Egelseer EM, Mayer HF, Sleytr UB, Sara MJ Bacteriol10.1128/JB.181.24.7643-7646.19991999Bacillus/*chemistry/ultrastructure, Bacterial Proteins/*metabolism, Carbohydrate Conformation, Cell Wall/*chemistry/ultrastructure, Crystallization, Magnetic Resonance Spectroscopy, Membrane Glycoproteins/*metabolism, Microscopy, Electron, Peptidoglycan/chemistry, Polymers/chemistry, Polysaccharides, Bacterial/*chemistry/metabolism, Structure-Activity RelationshipMetabolism
12089001Molecular characterization of the S-layer gene, sbpA, of Bacillus sphaericus CCM 2177 and production of a functional S-layer fusion protein with the ability to recrystallize in a defined orientation while presenting the fused allergen.Ilk N, Vollenkle C, Egelseer EM, Breitwieser A, Sleytr UB, Sara MAppl Environ Microbiol10.1128/AEM.68.7.3251-3260.20022002Bacillus/*genetics, Bacterial Proteins/chemistry/*genetics, Cloning, Molecular, Copper/chemistry, Crystallization, Escherichia coli, Gene Expression, Immunohistochemistry, Membrane Glycoproteins/chemistry/*genetics, Monosaccharide Transport Proteins/*genetics/isolation & purification, Peptide Fragments/genetics, Polylysine/chemistry, Recombinant Fusion Proteins/chemistryEnzymologyBiotechnology
12643755Generation of a functional monomolecular protein lattice consisting of an s-layer fusion protein comprising the variable domain of a camel heavy chain antibody.Pleschberger M, Neubauer A, Egelseer EM, Weigert S, Lindner B, Sleytr UB, Muyldermans S, Sara MBioconjug Chem10.1021/bc025603+2003Animals, Antibodies/*chemistry/genetics, Bacillus/metabolism, Bacteria/chemistry, Bacterial Proteins/chemistry, Camelus/*immunology, Cell Wall/metabolism, Cloning, Molecular, Crystallization, Electrophoresis, Polyacrylamide Gel, Enzyme-Linked Immunosorbent Assay, Escherichia coli/metabolism, Immunoblotting, Immunohistochemistry, Microcomputers, Microscopy, Atomic Force, Muramidase/chemistry, Nanotechnology, Peptidoglycan/chemistry, Polystyrenes/chemistry, Recombinant Fusion Proteins/*chemistry/genetics, Surface Plasmon ResonanceEnzymologyMetabolism
14629556Self-assembly and recrystallization of bacterial S-layer proteins at silicon supports imaged in real time by atomic force microscopy.Gyorvary ES, Stein O, Pum D, Sleytr UBJ Microsc10.1111/j.1365-2818.2003.01270.x2003Bacillus/growth & development/*metabolism, *Bacterial Proteins, Crystallization, Microscopy, Atomic Force/*methods, Monosaccharide Transport Proteins/*chemistry/*metabolism, SiliconMetabolism
14725506A functional chimaeric S-layer-enhanced green fluorescent protein to follow the uptake of S-layer-coated liposomes into eukaryotic cells.Ilk N, Kupcu S, Moncayo G, Klimt S, Ecker RC, Hofer-Warbinek R, Egelseer EM, Sleytr UB, Sara MBiochem J10.1042/BJ200319002004Bacterial Proteins/*genetics/metabolism, Cloning, Molecular, Crystallization, Endocytosis, Green Fluorescent Proteins, HeLa Cells, Humans, Immunoblotting, Liposomes/chemistry/*metabolism, Luminescent Proteins/*analysis/chemistry/genetics, Membrane Glycoproteins/*genetics/metabolism, Microscopy, Confocal, Microscopy, Fluorescence, Molecular Sequence Data, Peptidoglycan/chemistry, Recombinant Fusion Proteins/analysis/chemistry/metabolism, Spectrometry, FluorescenceMetabolismEnzymology
15003878Highly robust lipid membranes on crystalline S-layer supports investigated by electrochemical impedance spectroscopy.Gufler PC, Pum D, Sleytr UB, Schuster BBiochim Biophys Acta10.1016/j.bbamem.2003.12.0092004Alamethicin/chemistry, Crystallization, Electrochemistry, *Lipid Bilayers, Spectrum Analysis/*methods
15006773Construction of a functional S-layer fusion protein comprising an immunoglobulin G-binding domain for development of specific adsorbents for extracorporeal blood purification.Vollenkle C, Weigert S, Ilk N, Egelseer E, Weber V, Loth F, Falkenhagen D, Sleytr UB, Sara MAppl Environ Microbiol10.1128/AEM.70.3.1514-1521.20042004Autoimmune Diseases/immunology/therapy, Bacterial Proteins/*biosynthesis/chemistry/*genetics, Base Sequence, Binding Sites, Cloning, Molecular, Crystallization, DNA, Recombinant/genetics, Humans, Immunoglobulin G/*isolation & purification/*metabolism, Immunosorbents, In Vitro Techniques, Membrane Glycoproteins/*biosynthesis/chemistry/*genetics, Microscopy, Atomic Force, Microscopy, Electron, Microspheres, Protein Structure, Tertiary, Recombinant Fusion Proteins/biosynthesis/chemistry/geneticsEnzymologyPathogenicity
15149195An S-layer heavy chain camel antibody fusion protein for generation of a nanopatterned sensing layer to detect the prostate-specific antigen by surface plasmon resonance technology.Pleschberger M, Saerens D, Weigert S, Sleytr UB, Muyldermans S, Sara M, Egelseer EMBioconjug Chem10.1021/bc049964w2004Animals, Antibodies/*chemistry, Biosensing Techniques/*methods, Camelus, Cloning, Molecular, Enzyme-Linked Immunosorbent Assay, Epitope Mapping, Gold/chemistry, Immunization, Male, Nanotechnology, Prostate-Specific Antigen/analysis/*blood, Recombinant Fusion Proteins/chemistry, Surface Plasmon Resonance/*methodsEnzymology
15612928The three S-layer-like homology motifs of the S-layer protein SbpA of Bacillus sphaericus CCM 2177 are not sufficient for binding to the pyruvylated secondary cell wall polymer.Huber C, Ilk N, Runzler D, Egelseer EM, Weigert S, Sleytr UB, Sara MMol Microbiol10.1111/j.1365-2958.2004.04351.x2005Amino Acid Sequence, Bacillus/*metabolism, Bacterial Proteins/genetics/*metabolism, Binding Sites, Biopolymers/metabolism, Cell Wall/chemistry/metabolism, Cloning, Molecular, Membrane Glycoproteins/genetics/*metabolism, Membrane Proteins/genetics/metabolism, Molecular Sequence Data, Monosaccharide Transport Proteins/genetics/*metabolism, Protein Binding, Protein Structure, Tertiary, Recombinant Fusion Proteins/genetics/metabolism, Sequence Deletion, Surface Plasmon ResonanceMetabolismEnzymology
16151207Novel surface layer protein genes in Bacillus sphaericus associated with unusual insertion elements.Pollmann K, Raff J, Schnorpfeil M, Radeva G, Selenska-Pobell SMicrobiology (Reading)10.1099/mic.0.28201-02005Bacillus/*genetics/growth & development/*metabolism, Bacterial Proteins/chemistry/genetics/*metabolism, Binding Sites, DNA Transposable Elements/*physiology, Gene Transfer, Horizontal, Genes, Bacterial, Membrane Glycoproteins/chemistry/genetics/*metabolism, Molecular Sequence Data, Transposases/geneticsMetabolismGenetics
17193454Recrystallization of bacterial S-layers on flat polyelectrolyte surfaces and hollow polyelectrolyte capsules.Toca-Herrera JL, Krastev R, Bosio V, Kupcu S, Pum D, Fery A, Sara M, Sleytr UBSmall10.1002/smll.2004000352005Bacillus/metabolism, Buffers, Capsules/chemistry, Crystallization, Electrochemistry/methods, Electrolytes/*chemistry, Microscopy, Atomic Force, Microscopy, Confocal, Models, Chemical, Nanocapsules/*chemistry, Nanotechnology/instrumentation/*methods, Proteins/*chemistry, Silicon/chemistry, Surface PropertiesMetabolism
17193570Heterotetramers formed by an S-layer-streptavidin fusion protein and core-streptavidin as a nanoarrayed template for biochip development.Huber C, Liu J, Egelseer EM, Moll D, Knoll W, Sleytr UB, Sara MSmall10.1002/smll.2005001472006Biotinylation, Crystallization/*methods, Dimerization, In Situ Hybridization, Fluorescence/*methods, Materials Testing, Molecular Conformation, Multiprotein Complexes/chemistry, Nanostructures/*chemistry/ultrastructure, Nanotechnology/instrumentation/*methods, Oligonucleotide Array Sequence Analysis/instrumentation/*methods, Particle Size, Recombinant Fusion Proteins/chemistry, Streptavidin/*chemistry, Surface Plasmon Resonance/*methods, Surface PropertiesTranscriptome
18001070High-affinity tags fused to s-layer proteins probed by atomic force microscopy.Tang J, Ebner A, Ilk N, Lichtblau H, Huber C, Zhu R, Pum D, Leitner M, Pastushenko V, Gruber HJ, Sleytr UB, Hinterdorfer PLangmuir10.1021/la702276k2007Crystallization, Membrane Glycoproteins/*chemistry, Microscopy, Atomic Force/*methods, Polyethylene Glycols/chemistry, Silicon/chemistry, Streptavidin/chemistry, Surface Properties
18035441Exploitation of the S-layer self-assembly system for site directed immobilization of enzymes demonstrated for an extremophilic laminarinase from Pyrococcus furiosus.Tschiggerl H, Breitwieser A, de Roo G, Verwoerd T, Schaffer C, Sleytr UBJ Biotechnol10.1016/j.jbiotec.2007.09.0182007Archaeal Proteins/isolation & purification/*metabolism/ultrastructure, Biotechnology/*methods, Catalysis/drug effects, Cellulase/isolation & purification/*metabolism/ultrastructure, Cross-Linking Reagents/pharmacology, Crystallization, Enzyme Stability/drug effects, Enzymes, Immobilized/*metabolism, Membrane Glycoproteins/*metabolism, Membranes, Artificial, Pyrococcus furiosus/drug effects/*enzymology, Recombinant Fusion Proteins/ultrastructure, TemperatureEnzymologyMetabolism
18283801Mapping bacterial surface layers affinity to polyelectrolytes through the building of hybrid macromolecular structures.Delcea M, Krastev R, Gutlebert T, Pum D, Sleytr UB, Toca-Herrera JLJ Nanosci Nanotechnol10.1166/jnn.2007.9122007Bacteria/*chemistry, Electrolytes/*chemistry, Macromolecular Substances/*chemistry, Microscopy, Atomic Force
18376854Display of a peptide mimotope on a crystalline bacterial cell surface layer (S-layer) lattice for diagnosis of Epstein-Barr virus infection.Tschiggerl H, Casey JL, Parisi K, Foley M, Sleytr UBBioconjug Chem10.1021/bc70035232008Amino Acid Sequence, Bacillus/genetics/*metabolism, *Biomimetic Materials/chemistry/metabolism, Cross Reactions, Enzyme-Linked Immunosorbent Assay/*methods, Epstein-Barr Virus Infections/*diagnosis/immunology, Gene Expression, *Immunodominant Epitopes/chemistry/immunology/metabolism, Membrane Glycoproteins/genetics/*metabolism, Molecular Sequence Data, *Peptides/chemistry/immunology/metabolism, Recombinant Fusion Proteins/chemistry/immunology/metabolism, Sensitivity and SpecificityMetabolismGenetics
18626578Cloning, protein sequence clarification, and substrate specificity of a leucine dehydrogenase from Bacillus sphaericus ATCC4525.Li H, Zhu D, Hyatt BA, Malik FM, Biehl ER, Hua LAppl Biochem Biotechnol10.1007/s12010-008-8304-22008Amino Acid Sequence, Amino Acids/metabolism, Bacillus/*enzymology/genetics, Catalysis, Cloning, Molecular, Electrophoresis, Polyacrylamide Gel, Kinetics, Leucine Dehydrogenase/*chemistry/genetics/*metabolism, Molecular Sequence Data, Recombinant Proteins/chemistry/genetics/metabolism, Sequence Analysis, DNA, Sequence Homology, Amino Acid, Substrate SpecificityEnzymologyMetabolism
18642859Self-assembly of S-layer-enveloped cytochrome c polyelectrolyte multilayers.Dronov R, Kurth DG, Mohwald H, Scheller FW, Friedmann J, Pum D, Sleytr UB, Lisdat FLangmuir10.1021/la80069582008Animals, Crystallization, Cytochromes c/*chemistry/metabolism/ultrastructure, Electrolytes/*chemistry, Horses, Microscopy, Atomic ForceMetabolism
19232541Detection of metal binding sites on functional S-layer nanoarrays using single molecule force spectroscopy.Tang J, Ebner A, Kraxberger B, Leitner M, Hykollari A, Kepplinger C, Grunwald C, Gruber HJ, Tampe R, Sleytr UB, Ilk N, Hinterdorfer PJ Struct Biol10.1016/j.jsb.2009.02.0032009Bacterial Proteins/chemistry, Binding Sites, Membrane Glycoproteins/*chemistry, Microscopy, Atomic Force, Nanotechnology/*methods
19402706Genetic engineering of the S-layer protein SbpA of Lysinibacillus sphaericus CCM 2177 for the generation of functionalized nanoarrays.Badelt-Lichtblau H, Kainz B, Vollenkle C, Egelseer EM, Sleytr UB, Pum D, Ilk NBioconjug Chem10.1021/bc800445r2009Animals, Bacillaceae/*genetics, Bacterial Proteins/chemistry/*genetics/isolation & purification/metabolism, Carbonic Anhydrases/metabolism, Cloning, Molecular, Crystallization, Cysteine/metabolism, Fluorescent Dyes/metabolism, Genetic Engineering/*methods, Gold/chemistry/metabolism, Image Processing, Computer-Assisted, Metal Nanoparticles/chemistry, Microscopy, Electron, Transmission, Monosaccharide Transport Proteins/chemistry/*genetics/isolation & purification/metabolism, Nanoparticles/*chemistry, Rabbits, Recombinant Fusion Proteins/chemistry/genetics/isolation & purification/metabolism, Solubility, Surface Properties, Water/chemistryMetabolismEnzymology
19853422S-layer templated bioinspired synthesis of silica.Gobel C, Schuster B, Baurecht D, Sleytr UB, Pum DColloids Surf B Biointerfaces10.1016/j.colsurfb.2009.09.0372009Adsorption, Bacterial Proteins/*metabolism, Crystallization, Ethyldimethylaminopropyl Carbodiimide/chemistry, Microscopy, Electron, Transmission, Models, Chemical, Monosaccharide Transport Proteins/*metabolism, Protein Stability, Quartz/chemistry, Silicon Dioxide/*chemical synthesis/chemistry, Spectroscopy, Fourier Transform Infrared, Surface Properties, ViscosityMetabolismStress
21096866Self-assembly and recrystallization of bacterial S-layer proteins of Bacillus sphaericus and Bacillus thuringiensis on silicone, mica and quartz crystal supports.Habibi N, Caneva Soumetz F, Giulianelli M, Pastorino L, Herrera O, Sbrana F, Raiteri R, Ruggiero CAnnu Int Conf IEEE Eng Med Biol Soc10.1109/IEMBS.2010.56274992010Adsorption, Aluminum Silicates/*chemistry, Bacillus/*metabolism, Binding Sites, Crystallization/*methods, Materials Testing, Membrane Glycoproteins/*chemistry, Protein Binding, Quartz/*chemistry, Silicon/*chemistryMetabolism
21180722Electrochemical control of adsorption dynamics of surface layer proteins on gold.Zafiu C, Trettenhahn G, Pum D, Sleytr UB, Kautek WPhys Chem Chem Phys10.1039/c0cp01099e2010Adsorption, Bacillaceae/metabolism, Bacterial Proteins/*chemistry, Calcium/chemistry, Electrochemical Techniques, Gold/*chemistry, Surface PropertiesMetabolism
21310062Expression of an endotoxin-free S-layer/allergen fusion protein in gram-positive Bacillus subtilis 1012 for the potential application as vaccines for immunotherapy of atopic allergy.Ilk N, Schumi CT, Bohle B, Egelseer EM, Sleytr UBMicrob Cell Fact10.1186/1475-2859-10-62011Allergens/*genetics/immunology/metabolism, Antigens, Plant/biosynthesis/*genetics/immunology, Bacillus subtilis/*genetics/growth & development/metabolism, Betula/*immunology, Humans, Hypersensitivity/drug therapy/prevention & control, Immunoglobulin E/immunology, Immunotherapy, Membrane Glycoproteins/genetics/metabolism, Recombinant Fusion Proteins/*biosynthesis/genetics/immunology, Vaccines, Synthetic/biosynthesis/immunology/therapeutic useMetabolismPathogenicity
21698313Structural control of surface layer proteins at electrified interfaces investigated by in situ Fourier transform infrared spectroscopy.Zafiu C, Trettenhahn G, Pum D, Sleytr UB, Kautek WPhys Chem Chem Phys10.1039/c0cp02127j2011Adsorption, Calcium/chemistry, Membrane Glycoproteins/chemistry, Proteins/*chemistry, Spectroscopy, Fourier Transform Infrared, Surface Properties
21721841Multitechnique study on a recombinantly produced Bacillus halodurans laccase and an S-layer/laccase fusion protein.Ferner-Ortner-Bleckmann J, Schrems A, Ilk N, Egelseer EM, Sleytr UB, Schuster BBiointerphases10.1116/1.35892842011Bacillus/*enzymology/genetics, Cloning, Molecular, Enzymes, Immobilized/genetics/metabolism, Escherichia coli/genetics/metabolism, Gene Expression, Laccase/*genetics/*metabolism, Membrane Glycoproteins/*genetics/*metabolism, Recombinant Fusion Proteins/genetics/metabolismEnzymologyMetabolism
23443476Identification of a novel gene cluster in the upstream region of the S-layer gene sbpA involved in cell wall metabolism of Lysinibacillus sphaericus CCM 2177 and characterization of the recombinantly produced autolysin and pyruvyl transferase.Pleschberger M, Hildner F, Runzler D, Gelbmann N, Mayer HF, Sleytr UB, Egelseer EMArch Microbiol10.1007/s00203-013-0876-82013Bacillus/cytology/enzymology/*genetics/metabolism, Cell Wall/chemistry/genetics/metabolism, Escherichia coli/genetics, Membrane Glycoproteins/chemistry/genetics, *Multigene Family, N-Acetylmuramoyl-L-alanine Amidase/*genetics/metabolism, *Open Reading Frames, Peptidoglycan/chemistry/genetics/metabolism, Protein Structure, Tertiary, Sequence Analysis, DNA, Transferases/*genetics/metabolismMetabolismTranscriptome
23478879Nanobiotechnology advanced antifouling surfaces for the continuous electrochemical monitoring of glucose in whole blood using a lab-on-a-chip.Picher MM, Kupcu S, Huang CJ, Dostalek J, Pum D, Sleytr UB, Ertl PLab Chip10.1039/c3lc41308j2013Bacillaceae/chemistry, Bacterial Proteins/chemistry, Blood Glucose/*analysis, Blood Glucose Self-Monitoring/instrumentation/methods, *Electrochemical Techniques/instrumentation/methods, Humans, Membrane Glycoproteins/chemistry, *Microfluidic Analytical Techniques/instrumentation/methods, *Nanotechnology/instrumentation/methodsPathogenicityBiotechnology
23757161Surface-layer lattices as patterning element for multimeric extremozymes.Ferner-Ortner-Bleckmann J, Gelbmann N, Tesarz M, Egelseer EM, Sleytr UBSmall10.1002/smll.2012010142013Aldose-Ketose Isomerases/genetics/*metabolism, Bacterial Proteins/genetics/*metabolism, Catalysis, Crystallization, Membrane Glycoproteins/genetics/*metabolism, Recombinant Fusion Proteins/genetics/*metabolismMetabolism
28216572In Vitro Characterization of the Two-Stage Non-Classical Reassembly Pathway of S-Layers.Breitwieser A, Iturri J, Toca-Herrera JL, Sleytr UB, Pum DInt J Mol Sci10.3390/ijms180204002017Adsorption, Bacterial Proteins/chemistry/metabolism, Crystallization, Hydrophobic and Hydrophilic Interactions, Membrane Glycoproteins/*chemistry/*metabolism, Microscopy, Atomic Force, Monosaccharide Transport Proteins/chemistry/metabolism, Protein Binding, Protein Multimerization, Recombinant Proteins, Surface PropertiesMetabolism
28704713Cation-chelation and pH induced controlled switching of the non-fouling properties of bacterial crystalline films.Iturri J, Moreno-Cencerrado A, Toca-Herrera JLColloids Surf B Biointerfaces10.1016/j.colsurfb.2017.07.0032017Cations/*chemistry, Edetic Acid/chemistry, Human Umbilical Vein Endothelial Cells, Humans, Hydrogen-Ion Concentration, Membrane Glycoproteins/chemistry, Microscopy, Atomic Force, Quartz Crystal Microbalance TechniquesPathogenicity
32907106Thermal stability, mechanical properties and content of bacterial protein layers recrystallized on polyelectrolyte multilayers.Delcea M, Krastev R, Gutberlet T, Pum D, Sleytr UB, Toca-Herrera JLSoft Matter10.1039/b719408k2008
34065322A New Method for Dispersing Pristine Carbon Nanotubes Using Regularly Arranged S-Layer Proteins.Breitwieser A, Sleytr UB, Pum DNanomaterials (Basel)10.3390/nano110513462021

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@idauthorstitledoi/urlcatalogue
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
35731Curators of the CIPhttps://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20104788Collection of Institut Pasteur (CIP 104788)
68371Automatically annotated from API 50CH acid
68382Automatically annotated from API zym
93517Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID46447.1