Strain identifier
BacDive ID: 1361
Type strain: 
Species: Lentibacillus lacisalsi
Strain Designation: BH260
Strain history: <- C.-J. Kim <- J.-M. Lim <- D.-J. Park; BH260
NCBI tax ID(s): 284575 (species)
version 9 (current version)
General
@ref: 6453
BacDive-ID: 1361
DSM-Number: 16462
keywords: 16S sequence, Bacteria, obligate aerobe, moderately halophilic, spore-forming, Gram-positive, motile, rod-shaped, colony-forming
description: Lentibacillus lacisalsi BH260 is an obligate aerobe, moderately halophilic, spore-forming bacterium that forms circular colonies and was isolated from soil sediment of a salt lake.
NCBI tax id
- NCBI tax id: 284575
- Matching level: species
strain history
- @ref: 6453
- history: <- C.-J. Kim <- J.-M. Lim <- D.-J. Park; BH260
doi: 10.13145/bacdive1361.20240510.9
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/bacillota
- domain: Bacteria
- phylum: Bacillota
- class: Bacilli
- order: Caryophanales
- family: Bacillaceae
- genus: Lentibacillus
- species: Lentibacillus lacisalsi
- full scientific name: Lentibacillus lacisalsi Lim et al. 2005
@ref: 6453
domain: Bacteria
phylum: Firmicutes
class: Bacilli
order: Bacillales
family: Bacillaceae
genus: Lentibacillus
species: Lentibacillus lacisalsi
full scientific name: Lentibacillus lacisalsi Lim et al. 2005
strain designation: BH260
type strain: yes
Morphology
cell morphology
- @ref: 22998
- gram stain: positive
- cell length: 1.2-3.0 µm
- cell width: 0.4-0.6 µm
- cell shape: rod-shaped
- motility: yes
- flagellum arrangement: peritrichous
colony morphology
- @ref: 22998
- colony color: cream
- colony shape: circular
- medium used: MA supplemented with 10 % (w/v) NaCl
Culture and growth conditions
culture medium
| @ref | name | growth | link | composition |
|---|---|---|---|---|
| 6453 | BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514) | yes | https://mediadive.dsmz.de/medium/514 | Name: BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514; with strain-specific modifications) Composition: NaCl 80.0 g/l MgCl2 5.9 g/l Bacto peptone 5.0 g/l Na2SO4 3.24 g/l CaCl2 1.8 g/l Yeast extract 1.0 g/l KCl 0.55 g/l NaHCO3 0.16 g/l Fe(III) citrate 0.1 g/l KBr 0.08 g/l SrCl2 0.034 g/l H3BO3 0.022 g/l Na2HPO4 0.008 g/l Na-silicate 0.004 g/l NaF 0.0024 g/l (NH4)NO3 0.0016 g/l Distilled water |
| 22998 | MA supplemented with 10 % (w/v) NaCl | yes |
culture temp
| @ref | growth | type | temperature |
|---|---|---|---|
| 6453 | positive | growth | 30 |
| 22998 | positive | growth | 15.0-40.0 |
| 22998 | positive | optimum | 30.0-32.0 |
culture pH
| @ref | ability | type | pH | PH range |
|---|---|---|---|---|
| 22998 | positive | growth | 7.0-9.5 | alkaliphile |
| 22998 | positive | optimum | 8.0 |
Physiology and metabolism
oxygen tolerance
- @ref: 22998
- oxygen tolerance: obligate aerobe
spore formation
- @ref: 22998
- spore description: spherical,terminal,in swollen sporangia
- type of spore: endospore
- spore formation: yes
halophily
| @ref | halophily level | salt | growth | tested relation | concentration |
|---|---|---|---|---|---|
| 22998 | moderately halophilic | NaCl | positive | growth | 5.0-25.0 %(w/v) |
| 22998 | NaCl | optimum | 12.0-15.0 % |
metabolite utilization
| @ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
|---|---|---|---|---|
| 22998 | 15963 | ribitol | - | builds acid from |
| 22998 | 36219 | alpha-lactose | - | builds acid from |
| 22998 | 18305 | arbutin | - | builds acid from |
| 22998 | 17634 | D-glucose | - | builds acid from |
| 22998 | 16899 | D-mannitol | - | builds acid from |
| 22998 | 16024 | D-mannose | - | builds acid from |
| 22998 | 28053 | melibiose | - | builds acid from |
| 22998 | 16551 | D-trehalose | - | builds acid from |
| 22998 | 17754 | glycerol | - | builds acid from |
| 22998 | 17306 | maltose | - | builds acid from |
| 22998 | 16634 | raffinose | - | builds acid from |
| 22998 | 26546 | rhamnose | - | builds acid from |
| 22998 | 17814 | salicin | - | builds acid from |
| 22998 | casein | - | hydrolysis | |
| 22998 | 4853 | esculin | - | hydrolysis |
| 22998 | 17368 | hypoxanthine | - | hydrolysis |
| 22998 | 17895 | L-tyrosine | - | hydrolysis |
| 22998 | 28017 | starch | - | hydrolysis |
| 22998 | 53426 | tween 80 | - | hydrolysis |
| 22998 | 16199 | urea | - | hydrolysis |
| 22998 | 15318 | xanthine | - | hydrolysis |
| 22998 | 17108 | D-arabinose | + | builds acid from |
| 22998 | 15824 | D-fructose | + | builds acid from |
| 22998 | 16988 | D-ribose | + | builds acid from |
| 22998 | 65327 | D-xylose | + | builds acid from |
| 22998 | 30849 | L-arabinose | + | builds acid from |
| 22998 | 17632 | nitrate | + | reduction |
enzymes
- @ref: 22998
- value: cytochrome oxidase
- activity: +
- ec: 1.9.3.1
Isolation, sampling and environmental information
isolation
| @ref | sample type | geographic location | country | origin.country | continent | enrichment culture | enrichment culture duration | enrichment culture temperature |
|---|---|---|---|---|---|---|---|---|
| 22998 | soil sediment of a salt lake | Xin-Jiang Province | China | CHN | Asia | marine agar 2216 (MA;Difco) with the addition of 20 % (w/v) NaCl [final concentration, 21.94 % (w/v) NaCl] | 3 days | 28.0 |
| 6453 | salt lake soil | China | CHN | Asia |
isolation source categories
| Cat1 | Cat2 | Cat3 |
|---|---|---|
| #Environmental | #Aquatic | #Lake (large) |
| #Environmental | #Terrestrial | #Sediment |
| #Environmental | #Terrestrial | #Soil |
| #Condition | #Saline |
taxonmaps
- @ref: 69479
- File name: preview.99_48902.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_3;96_22114;97_27617;98_35192;99_48902&stattab=map
- Last taxonomy: Lentibacillus lacisalsi subclade
- 16S sequence: AY667497
- Sequence Identity:
- Total samples: 26
- soil counts: 11
- aquatic counts: 2
- animal counts: 12
- plant counts: 1
Safety information
risk assessment
- @ref: 6453
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 22998
- description: Lentibacillus lacisalsi strain BH260 16S ribosomal RNA gene, partial sequence
- accession: AY667497
- length: 1365
- database: nuccore
- NCBI tax ID: 284575
GC content
| @ref | GC-content | method |
|---|---|---|
| 6453 | 44 | |
| 22998 | 44 | high performance liquid chromatography (HPLC) |
External links
@ref: 6453
culture collection no.: DSM 16462, KCTC 3915
straininfo link
- @ref: 71012
- straininfo: 232135
literature
| topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
|---|---|---|---|---|---|---|---|---|
| Phylogeny | 16166670 | Lentibacillus lacisalsi sp. nov., a moderately halophilic bacterium isolated from a saline lake in China. | Lim JM, Jeon CO, Song SM, Lee JC, Ju YJ, Xu LH, Jiang CL, Kim CJ | Int J Syst Evol Microbiol | 10.1099/ijs.0.63721-0 | 2005 | Bacillaceae/*classification/genetics/isolation & purification/physiology, Bacterial Typing Techniques, China, DNA, Bacterial/analysis, Fatty Acids/analysis, Fresh Water/*microbiology, Genes, rRNA, Molecular Sequence Data, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Sodium Chloride | Genetics |
| Phylogeny | 18676465 | Lentibacillus salis sp. nov., a moderately halophilic bacterium isolated from a salt lake. | Lee JC, Li WJ, Xu LH, Jiang CL, Kim CJ | Int J Syst Evol Microbiol | 10.1099/ijs.0.65545-0 | 2008 | Bacillaceae/*classification/genetics/isolation & purification/physiology, Bacterial Typing Techniques, Base Composition, China, DNA, Bacterial/analysis, DNA, Ribosomal/analysis, Fatty Acids/analysis, Fresh Water/*microbiology, Genes, rRNA, Molecular Sequence Data, Phenotype, Phylogeny, RNA, Ribosomal, 16S/genetics, *Sodium Chloride, Species Specificity | Genetics |
Reference
| @id | authors | catalogue | doi/url | title | journal | pubmed |
|---|---|---|---|---|---|---|
| 6453 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 16462) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-16462 | |||
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
| 22998 | Jee-Min Lim,Che Ok Jeon,Sung-Min Song,Jae-Chan Lee,Yoon Jung Ju,Li-Hua Xu,Cheng-Lin Jiang,Chang-Jin Kim | 10.1099/ijs.0.63721-0 | Lentibacillus lacisalsi sp. nov., a moderately halophilic bacterium isolated from a saline lake in China | IJSEM 55: 1805-1809 2005 | 16166670 | |
| 69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | https://microbeatlas.org/ | MicrobeAtlas 1.0 beta | |||
| 71012 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID232135.1 | StrainInfo: A central database for resolving microbial strain identifiers |