Strain identifier

BacDive ID: 135600

Type strain: No

Species: Cronobacter sakazakii

Strain Designation: F37/50

Strain history: CIP <- 1957, NCTC: strain F37/50 <- 1950, B.C. Hobbs, PHLS, UK, Bacterium cloacae

NCBI tax ID(s): 1367848 (strain), 28141 (species)

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General

@ref: 34774

BacDive-ID: 135600

keywords: genome sequence, Bacteria, aerobe, mesophilic, motile

description: Cronobacter sakazakii F37/50 is an aerobe, mesophilic, motile bacterium that was isolated from Tin of Milk.

NCBI tax id

NCBI tax idMatching level
1367848strain
28141species

strain history

  • @ref: 34774
  • history: CIP <- 1957, NCTC: strain F37/50 <- 1950, B.C. Hobbs, PHLS, UK, Bacterium cloacae

doi: 10.13145/bacdive135600.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Enterobacterales
  • family: Enterobacteriaceae
  • genus: Cronobacter
  • species: Cronobacter sakazakii
  • full scientific name: Cronobacter sakazakii (Farmer et al. 1980) Iversen et al. 2008

  • synonyms

    @refsynonym
    20215Enterobacter sakazakii
    20215Cronobacter sakazakii

@ref: 34774

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Enterobacterales

family: Enterobacteriaceae

genus: Cronobacter

species: Cronobacter sakazakii

strain designation: F37/50

type strain: no

Morphology

cell morphology

@refmotilityconfidencegram staincell shape
69480yes95.281
6948099.917negative
34774yesnegativerod-shaped

Culture and growth conditions

culture medium

@refnamegrowthcompositionlink
34774MEDIUM 72- for trypto casein soja agaryesDistilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g)
34774CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72

culture temp

@refgrowthtypetemperaturerange
34774positivegrowth30mesophilic
45340positivegrowth37mesophilic
34774positivegrowth30-41
34774nogrowth5psychrophilic
34774nogrowth10psychrophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
45340aerobe
34774facultative anaerobe

spore formation

@refspore formationconfidence
69481no100
69480no99.346
34774no

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6836830849L-arabinose+fermentation
6836827613amygdalin+fermentation
6836828053melibiose+fermentation
6836817992sucrose+fermentation
6836862345L-rhamnose+fermentation
6836830911sorbitol-fermentation
6836817268myo-inositol+fermentation
6836816899D-mannitol+fermentation
6836817634D-glucose+fermentation
683685291gelatin-hydrolysis
6836827897tryptophan-energy source
6836816199urea-hydrolysis
6836816947citrate+assimilation
6836818257ornithine+degradation
6836825094lysine-degradation
6836829016arginine+hydrolysis
3477429864mannitol+fermentation
3477416947citrate+carbon source
3477417234glucose+fermentation
3477417716lactose+fermentation
3477417632nitrate+reduction
3477416301nitrite-reduction
3477415792malonate-assimilation
34774132112sodium thiosulfate-builds gas from
6837418257ornithine+degradation
6837429016arginine+hydrolysis
6837425094lysine-degradation
6837416199urea-hydrolysis
6837418403L-arabitol-builds acid from
6837418024D-galacturonic acid+builds acid from
68374Potassium 5-ketogluconate-builds acid from
6837416899D-mannitol+builds acid from
6837417306maltose+builds acid from
6837415963ribitol-builds acid from
6837418394palatinose-builds acid from
6837415792malonate-assimilation
6837427897tryptophan-energy source
6837417634D-glucose+builds acid from
6837417992sucrose+builds acid from
6837430849L-arabinose+builds acid from
6837418333D-arabitol-builds acid from
6837427082trehalose+builds acid from
6837462345L-rhamnose+builds acid from
6837417268myo-inositol+builds acid from
6837417057cellobiose+builds acid from
6837430911sorbitol-builds acid from

antibiotic resistance

  • @ref: 34774
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: yes
  • is resistant: no

metabolite production

@refChebi-IDmetaboliteproduction
6837435581indoleno
6836815688acetoinyes
6836835581indoleno
6836816136hydrogen sulfideno
3477435581indoleno

metabolite tests

@refChebi-IDmetaboliteindole testvoges-proskauer-testmethylred-test
6837435581indole-
6836815688acetoin+
6836835581indole-
3477415688acetoin+
3477417234glucose-

enzymes

@refvalueactivityec
68374L-aspartate arylamidase-3.4.11.21
68374alpha-maltosidase+
68374alpha-galactosidase+3.2.1.22
68374alpha-glucosidase+3.2.1.20
68374beta-galactosidase+3.2.1.23
68374N-acetyl-beta-glucosaminidase+3.2.1.52
68374beta-glucuronidase-3.2.1.31
68374beta-glucosidase+3.2.1.21
68374lipase+
68374urease-3.5.1.5
68374lysine decarboxylase-4.1.1.18
68374arginine dihydrolase+3.5.3.6
68374ornithine decarboxylase+4.1.1.17
68368cytochrome oxidase-1.9.3.1
68368gelatinase-
68368tryptophan deaminase-4.1.99.1
68368urease-3.5.1.5
68368ornithine decarboxylase+4.1.1.17
68368lysine decarboxylase-4.1.1.18
68368arginine dihydrolase+3.5.3.6
68368beta-galactosidase+3.2.1.23
34774oxidase-
34774beta-galactosidase+3.2.1.23
34774alcohol dehydrogenase+1.1.1.1
34774gelatinase-
34774catalase+1.11.1.6
34774lysine decarboxylase-4.1.1.18
34774ornithine decarboxylase+4.1.1.17
34774phenylalanine ammonia-lyase-4.3.1.24
34774tryptophan deaminase-
34774urease-3.5.1.5
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase+
68382cystine arylamidase-3.4.11.3
68382trypsin+3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase-
68382alpha-galactosidase+3.2.1.22
68382beta-galactosidase+3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase+3.2.1.20
68382beta-glucosidase+3.2.1.21
68382N-acetyl-beta-glucosaminidase+3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
34774-+++-++-+-+-++-+++--

API 20E

@refONPGADH ArgLDC LysODCCITH2SURETDA TrpINDVPGELGLUMANINOSorRHASACMELAMYARAOX
45340++-++----+-+++-+++++-

API ID32E

@refODCADH ArgLDC LysURELARLGAT5KGLIPRPbeta GLUMANMALADOPLEbeta GURMNTINDbeta NAGbeta GALGLUSACLARADARLalpha GLUalpha GALTRERHAINOCELSORalphaMALAspA
45340++---+-+++++-----+++++-++++++-+-

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
34774+++++-++++++-+++++++++++-------+++++-----+-------+++-++++--++-----------++-+---++-----+--++++++----

Isolation, sampling and environmental information

isolation

  • @ref: 45340
  • sample type: Tin of Milk

isolation source categories

Cat1Cat2Cat3
#Engineered#Food production#Bottled, canned, packed food
#Engineered#Food production#Dairy product
#Host Body Product#Fluids#Milk

Safety information

risk assessment

  • @ref: 34774
  • biosafety level: 2
  • biosafety level comment: Risk group (French classification)

Sequence information

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Cronobacter sakazakii NCTC 8155GCA_001277275completencbi28141
66792Cronobacter sakazakii NCIMB 8272GCA_000463095scaffoldncbi1367848
66792Cronobacter sakazakii NCIMB 82721367848.3wgspatric1367848
66792Cronobacter sakazakii strain NCTC 815528141.372plasmidpatric28141
66792Cronobacter sakazakii strain NCTC 815528141.371plasmidpatric28141
66792Cronobacter sakazakii strain NCTC 815528141.62completepatric28141
66792Cronobacter sakazakii strain NCTC 815528141.370plasmidpatric28141
66792Cronobacter sakazakii NCIMB 82722602041574draftimg1367848
66792Cronobacter sakazakii NCTC 81552841645194completeimg28141

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno100no
motileyes91.73no
flagellatedno75.134no
gram-positiveno97.961no
anaerobicno97.093yes
halophileno79.017no
spore-formingno92.986no
thermophileno99.16no
glucose-utilyes95.693no
aerobicyes81.413yes
glucose-fermentyes91.799yes

External links

@ref: 34774

culture collection no.: CIP 57.33, NCTC 8155, NCIMB 8272, CCUG 10788

straininfo link

  • @ref: 92925
  • straininfo: 53506

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Metabolism19350978Evaluation of an antimicrobial ingredient prepared from a Lactobacillus acidophilus casein fermentate against Enterobacter sakazakii.Hayes M, Barrett E, Ross RP, Fitzgerald GF, Hill C, Stanton CJ Food Prot10.4315/0362-028x-72.2.3402009Caseins/metabolism/*pharmacology, Colony Count, Microbial, Cronobacter sakazakii/*drug effects/growth & development, Dose-Response Relationship, Drug, Fermentation, Food Contamination/*analysis, Humans, Infant, Infant Food/*microbiology, Infant Formula, Infant, Newborn, Lactobacillus acidophilus/metabolism/*physiology, Microbial Sensitivity Tests, Peptide Fragments/metabolism/pharmacologyPathogenicity
Biotechnology21111503Inactivation of Cronobacter sakazakii by ultrasonic waves under pressure in buffer and foods.Arroyo C, Cebrian G, Pagan R, Condon SInt J Food Microbiol10.1016/j.ijfoodmicro.2010.10.0332010Buffers, Cronobacter sakazakii/growth & development/*physiology, *Food Microbiology/methods, Food Technology/*methods, Hydrogen-Ion Concentration, *Microbial Viability, *Pressure, Temperature, Time Factors, *Ultrasonics, Water/chemistry
Genetics24072871Draft Genome Sequence of the Earliest Cronobacter sakazakii Sequence Type 4 Strain, NCIMB 8272.Masood N, Moore K, Farbos A, Hariri S, Paszkiewicz K, Dickins B, McNally A, Forsythe SGenome Announc10.1128/genomeA.00782-132013

Reference

@idauthorstitledoi/urlcatalogue
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
34774Curators of the CIPhttps://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%2057.33Collection of Institut Pasteur (CIP 57.33)
45340Curators of the CCUGhttps://www.ccug.se/strain?id=10788Culture Collection University of Gothenburg (CCUG) (CCUG 10788)
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
68368Automatically annotated from API 20E
68374Automatically annotated from API ID32E
68382Automatically annotated from API zym
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. MünchPredictions based on the model GenomeNet Sporulation v. 110.21203/rs.3.rs-2527258/v1
92925Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID53506.1