Strain identifier

BacDive ID: 135306

Type strain: No

Species: Lactobacillus acidophilus

Strain Designation: 4B

Strain history: CIP <- 1992, F. Gasser, Inst. Pasteur, Paris, France: strain VPI 0326, Lactobacillus acidophilus, group A1 <- ATCC <- 1923, L.F. Rettger, Yale: strain 4B <- W.L. Kulp

NCBI tax ID(s): 1579 (species)

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General

@ref: 34390

BacDive-ID: 135306

keywords: 16S sequence, Bacteria, facultative anaerobe, mesophilic, Gram-positive, rod-shaped

description: Lactobacillus acidophilus 4B is a facultative anaerobe, mesophilic, Gram-positive bacterium that was isolated from Rat feces.

NCBI tax id

  • NCBI tax id: 1579
  • Matching level: species

strain history

@refhistory
67770T. Mitsuoka S1-68 <-- M. E. Sharpe A-1 <-- NCTC 1723 <-- L. F. Rettger 4B <-- W. L. Kulp.
34390CIP <- 1992, F. Gasser, Inst. Pasteur, Paris, France: strain VPI 0326, Lactobacillus acidophilus, group A1 <- ATCC <- 1923, L.F. Rettger, Yale: strain 4B <- W.L. Kulp

doi: 10.13145/bacdive135306.20250331.9.3

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Lactobacillales
  • family: Lactobacillaceae
  • genus: Lactobacillus
  • species: Lactobacillus acidophilus
  • full scientific name: Lactobacillus acidophilus (Moro 1900) Hansen and Mocquot 1970 (Approved Lists 1980)

  • synonyms

    • @ref: 20215
    • synonym: Bacillus acidophilus

@ref: 34390

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Lactobacillales

family: Lactobacillaceae

genus: Lactobacillus

species: Lactobacillus acidophilus

strain designation: 4B

type strain: no

Morphology

cell morphology

  • @ref: 34390
  • gram stain: positive
  • cell shape: rod-shaped
  • motility: no

Culture and growth conditions

culture medium

@refnamegrowthcompositionlink
34390MEDIUM 40- for Lactobacillus and LeuconostocyesDistilled water make up to (1000.000 ml);Man Rogosa Sharp agar (68.000 g)
34390CIP Medium 40yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=40

culture temp

@refgrowthtypetemperature
34390positivegrowth37
67770positivegrowth37
34390positivegrowth30-45
34390nogrowth5
34390nogrowth10

Physiology and metabolism

oxygen tolerance

  • @ref: 34390
  • oxygen tolerance: facultative anaerobe

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
3439017632nitrate+respiration
68371Potassium 5-ketogluconate-builds acid from
6837127613amygdalin+builds acid from
68371320061methyl alpha-D-glucopyranoside-builds acid from
6837116024D-mannose-builds acid from
6837115824D-fructose+builds acid from
6837117634D-glucose+builds acid from
68371Potassium 2-ketogluconate-builds acid from
6837124265gluconate-builds acid from
6837118403L-arabitol-builds acid from
6837118333D-arabitol-builds acid from
6837118287L-fucose-builds acid from
6837128847D-fucose-builds acid from
6837116443D-tagatose-builds acid from
6837162318D-lyxose-builds acid from
6837128066gentiobiose+builds acid from
6837117151xylitol-builds acid from
6837128087glycogen-builds acid from
6837128017starch-builds acid from
6837116634raffinose+builds acid from
683716731melezitose-builds acid from
6837115443inulin-builds acid from
6837127082trehalose-builds acid from
6837117992sucrose+builds acid from
6837117716lactose+builds acid from
6837117306maltose+builds acid from
6837117057cellobiose+builds acid from
6837117814salicin+builds acid from
683714853esculin+builds acid from
6837118305arbutin-builds acid from
6837159640N-acetylglucosamine+builds acid from
6837143943methyl alpha-D-mannoside-builds acid from
6837117924D-sorbitol-builds acid from
6837116899D-mannitol-builds acid from
6837117268myo-inositol-builds acid from
6837116813galactitol-builds acid from
6837162345L-rhamnose-builds acid from
6837117266L-sorbose-builds acid from
6837174863methyl beta-D-xylopyranoside-builds acid from
6837115963ribitol-builds acid from
6837165328L-xylose-builds acid from
6837165327D-xylose-builds acid from
6837116988D-ribose-builds acid from
6837130849L-arabinose-builds acid from
6837117108D-arabinose-builds acid from
6837117113erythritol-builds acid from
6837117754glycerol-builds acid from

enzymes

@refvalueactivityec
34390oxidase-
34390alcohol dehydrogenase-1.1.1.1
34390catalase-1.11.1.6
34390lysine decarboxylase-4.1.1.18
34390ornithine decarboxylase-4.1.1.17

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
34390---------+/-++---------++-++++++/-+---+---++/----------

Isolation, sampling and environmental information

isolation

@refsample typeisolation date
67770Rat feces
34390Animal, Feces, white rat1922

Safety information

risk assessment

  • @ref: 34390
  • biosafety level: 1
  • biosafety level comment: Risk group (French classification)

Sequence information

16S sequences

  • @ref: 124043
  • description: Lactobacillus acidophilus gene for 16S rRNA, partial sequence, strain: JCM 1023.
  • accession: AB289012
  • length: 675
  • database: nuccore
  • NCBI tax ID: 1579

GC content

  • @ref: 67770
  • GC-content: 34
  • method: thermal denaturation, midpoint method (Tm)

External links

@ref: 34390

culture collection no.: CIP 103597, ATCC 832, NCDO 1, NCTC 1723, NCIMB 1723, JCM 1023, KCTC 3142, LMG 11428, VPI 11760-B

straininfo link

  • @ref: 92680
  • straininfo: 13694

literature

topicPubmed-IDtitleauthorsjournalyearmeshtopic2DOI
Enzymology6803101Properties of alpha-glucosidase from Lactobacillus acidophilus NCTC 1723.Chan K, Li KBMicrobios1981Glucan 1,4-alpha-Glucosidase/analysis, Glucosidases/*analysis, Hydrogen-Ion Concentration, Isomaltose/analysis, Kinetics, Lactobacillus acidophilus/*enzymology, Temperature, alpha-Glucosidases/*analysis/isolation & purificationPhylogeny
Metabolism20461509Genetic and functional aspects of linoleate isomerase in Lactobacillus acidophilus.Macouzet M, Robert N, Lee BHAppl Microbiol Biotechnol2010Bacterial Proteins/*genetics/*metabolism, Chromatography, Gas, DNA, Bacterial/chemistry/genetics, Immunoblotting, Isomerases/*genetics/*metabolism, Lactobacillus acidophilus/*enzymology, Linoleic Acid/*metabolism, Molecular Sequence Data, Polymerase Chain Reaction, Sequence Analysis, DNA, Sequence HomologyGenetics10.1007/s00253-010-2634-z

Reference

@idauthorstitledoi/urlcatalogue
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
34390Curators of the CIPhttps://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20103597Collection of Institut Pasteur (CIP 103597)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68371Automatically annotated from API 50CH acid
92680Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID13694.1
124043Isabel Schober, Julia KoblitzData extracted from sequence databases, automatically matched based on designation and taxonomy