Name: Lactobacillus acidophilus 4B
Creator: BacDive
License: https://creativecommons.org/licenses/by/4.0/

Strain identifier

BacDive ID: 135306

Type strain:

Species: Lactobacillus acidophilus

Strain Designation: 4B

Culture col. no.: CIP 103597, ATCC 832, NCDO 1, NCTC 1723, NCIMB 1723, JCM 1023, KCTC 3142, LMG 11428, VPI 11760-B

Strain history: CIP <- 1992, F. Gasser, Inst. Pasteur, Paris, France: strain VPI 0326, Lactobacillus acidophilus, group A1 <- ATCC <- 1923, L.F. Rettger, Yale: strain 4B <- W.L. Kulp

NCBI tax ID(s): 1579 (species)

SI-ID 13694

LMG 11428, ATCC 832, JCM 1023, NCFB 1, NCIMB 1723, NCTC 1723, NCDO 1, NCIB 1723, CIP 103597, KCTC 3142, CECT 4179

Special Collections

Other strains from Lactobacillus acidophilus

Section

Lactobacillus acidophilus 4B is a facultative anaerobe, Gram-positive, rod-shaped bacterium that was isolated from Rat feces.

Gram-positive
rod-shaped
facultative anaerobe
16S sequence
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Bacilli*
	Order	*Lactobacillales*
	Family	*Lactobacillaceae*
	Genus	*Lactobacillus*
	Species	**Lactobacillus acidophilus**
	Full Scientific Name (LPSN)	Lactobacillus acidophilus (Moro 1900) Hansen and Mocquot 1970 (Approved Lists 1980)

Synonym

"Bacillus acidophilus"

Information on morphological and physiological properties Morphology

[Ref.: #34390]	Gram stain	positive

[Ref.: #34390]	Cell shape	rod-shaped

[Ref.: #34390]	Motility	no

Information on culture and growth conditions Culture and growth conditions

[Ref.: #34390]

Culture medium

MEDIUM 40- for Lactobacillus and Leuconostoc

[Ref.: #34390]

Culture medium growth

[Ref.: #34390]

Culture medium composition

expand / minimize

[Ref.: #34390]

Culture medium

CIP Medium 40

[Ref.: #34390]

Culture medium growth

[Ref.: #34390]

Culture medium link

Medium recipe provided by DSMZ

	Kind of temperature		Temperature
[Ref.: #34390]		growth	37 °C
[Ref.: #34390]		growth	30-45 °C
[Ref.: #34390]	no	growth	5 °C
[Ref.: #34390]	no	growth	10 °C
[Ref.: #67770]		growth	37 °C

Information on physiology and metabolism Physiology and metabolism

[Ref.: #34390]

Oxygen tolerance

facultative anaerobe

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68371]	amygdalin ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	arbutin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	cellobiose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-arabinose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-arabitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-fructose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-fucose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-glucose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-lyxose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-mannitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-mannose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-ribose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-sorbitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-tagatose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-xylose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	erythritol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	esculin ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	galactitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	gentiobiose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	gluconate ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	glycerol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	glycogen ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	inulin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-arabinose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-arabitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-fucose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-rhamnose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-sorbose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-xylose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	lactose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	maltose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	melezitose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	methyl alpha-D-glucopyranoside ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	methyl alpha-D-mannoside ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	methyl beta-D-xylopyranoside ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	myo-inositol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	N-acetylglucosamine ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #34390]	nitrate ChEBI	+	respiration
[Ref.: #68371]	Potassium 2-ketogluconate ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	Potassium 5-ketogluconate ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	raffinose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	ribitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	salicin ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	starch ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	sucrose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	trehalose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	xylitol ChEBI	-	builds acid from	* from API 50CH acid
	Only first 10 entries are displayed. Click here to see all.

	Enzyme	Enzyme activity	EC number
[Ref.: #34390]	alcohol dehydrogenase	-	1.1.1.1
[Ref.: #34390]	catalase	-	1.11.1.6
[Ref.: #34390]	lysine decarboxylase	-	4.1.1.18
[Ref.: #34390]	ornithine decarboxylase	-	4.1.1.17
[Ref.: #34390]	oxidase	-

API® 50CH acid:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	ControlQ	Acid from glycerolGLY	Acid from erythritolERY	Acid from D-arabinoseDARA	Acid from L-arabinoseLARA	Acid from D-riboseRIB	Acid from D-xyloseDXYL	Acid from L-xyloseLXYL	Acid from D-adonitolADO	Acid from methyl beta-D-xylopyranosideMDX	Acid from D-galactoseGAL	Acid from D-glucoseGLU	Acid from D-fructoseFRU	Acid from D-mannoseMNE	Acid from L-sorboseSBE	Acid from L-rhamnoseRHA	Acid from dulcitolDUL	Acid from inositolINO	Acid from D-mannitolMAN	Acid from D-sorbitolSOR	Acid from methyl alpha-D-mannopyranosideMDM	Acid from methyl alpha-D-glucopyranosideMDG	Acid from N-acetyl-glucosamineNAG	Acid from amygdalinAMY	Acid from arbutinARB	Acid from esculinESC	Acid from salicinSAL	Acid from cellobioseCEL	Acid from maltoseMAL	Acid from lactoseLAC	Acid from melibioseMEL	Acid from sucroseSAC	Acid from trehaloseTRE	Acid from inulinINU	Acid from melezitoseMLZ	Acid from raffinoseRAF	Acid from starchAMD	Acid from glycogenGLYG	Acid from xylitolXLT	Acid from gentiobioseGEN	Acid from turanoseTUR	Acid from D-lyxoseLYX	Acid from D-tagatoseTAG	Acid from D-fucoseDFUC	Acid from L-fucoseLFUC	Acid from D-arabitolDARL	Acid from L-arabitolLARL	Acid from gluconateGNT	Acid from 2-Ketogluconate2KG	Acid from 5-Ketogluconate5KG
[Ref.: #34390]	9319	not determinedn.d.	-	-	-	-	-	-	-	-	-	+/-	+	+	-	-	-	-	-	-	-	-	-	+	+	-	+	+	+	+	+	+/-	+	-	-	-	+	-	-	-	+	+/-	-	-	-	-	-	-	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #67770]	Sample type/isolated from	Rat feces

[Ref.: #34390]	Sample type/isolated from	Animal, Feces, white rat
[Ref.: #34390]	Isolation date	1922
* marker position based on {}

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #34390]

Biosafety level

Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #124043]	Lactobacillus acidophilus gene for 16S rRNA, partial sequence, strain: JCM 1023.	AB289012	675	GenBank ENA	1579 tax ID	*

[Ref.: #67770]

GC-content

34 mol%

thermal denaturation, midpoint method (Tm)

Availability in culture collections External links

[Ref.: #34390]

Culture collection no.

CIP 103597, ATCC 832, NCDO 1, NCTC 1723, NCIMB 1723, JCM 1023, KCTC 3142, LMG 11428, VPI 11760-B

[Ref.: #92680]

SI-ID 13694

Literature:

Topic	Title	Authors	Journal	DOI	Year
Metabolism	Genetic and functional aspects of linoleate isomerase in Lactobacillus acidophilus.	Macouzet M, Robert N, Lee BH	Appl Microbiol Biotechnol	10.1007/s00253-010-2634-z	2010	*
Enzymology	Properties of alpha-glucosidase from Lactobacillus acidophilus NCTC 1723.	Chan K, Li KB	Microbios		1981	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#34390 Collection of Institut Pasteur ; Curators of the CIP; CIP 103597
#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68371

Automatically annotated from API 50CH acid .

#92680

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID13694.1 )

#124043

Dr. Isabel Schober, Dr. Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy .

* These data were automatically processed and therefore are not curated

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