Strain identifier

BacDive ID: 13465

Type strain: Yes

Species: Saccharopolyspora cebuensis

Strain history: CIP <- 2006, S.M. Pimentel-Elardo, Würzburg Univ., Würzburg, Germany: strain SPE 10-1

NCBI tax ID(s): 418759 (species)

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General

@ref: 12249

BacDive-ID: 13465

DSM-Number: 45019

keywords: 16S sequence, Bacteria, aerobe, spore-forming, mesophilic, Gram-positive

description: Saccharopolyspora cebuensis DSM 45019 is an aerobe, spore-forming, mesophilic bacterium that was isolated from marine sponge.

NCBI tax id

  • NCBI tax id: 418759
  • Matching level: species

strain history

@refhistory
12249<- S. Pimentel-Elardo; SPE 10-1
67770CIP 109355 <-- S. M. Pimentel-Elardo SPE 10-1.
117226CIP <- 2006, S.M. Pimentel-Elardo, Würzburg Univ., Würzburg, Germany: strain SPE 10-1

doi: 10.13145/bacdive13465.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Pseudonocardiales
  • family: Pseudonocardiaceae
  • genus: Saccharopolyspora
  • species: Saccharopolyspora cebuensis
  • full scientific name: Saccharopolyspora cebuensis Pimentel-Elardo et al. 2008

@ref: 12249

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Pseudonocardiaceae

genus: Saccharopolyspora

species: Saccharopolyspora cebuensis

full scientific name: Saccharopolyspora cebuensis Pimentel-Elardo et al. 2008

type strain: yes

Morphology

cell morphology

@refgram staincell shapemotility
32105positive
117226positiverod-shapedno

pigmentation

  • @ref: 32105
  • production: yes

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
12249BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514)yeshttps://mediadive.dsmz.de/medium/514Name: BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514) Composition: NaCl 19.45 g/l MgCl2 5.9 g/l Bacto peptone 5.0 g/l Na2SO4 3.24 g/l CaCl2 1.8 g/l Yeast extract 1.0 g/l KCl 0.55 g/l NaHCO3 0.16 g/l Fe(III) citrate 0.1 g/l KBr 0.08 g/l SrCl2 0.034 g/l H3BO3 0.022 g/l Na2HPO4 0.008 g/l Na-silicate 0.004 g/l NaF 0.0024 g/l (NH4)NO3 0.0016 g/l Distilled water
33294MEDIUM 683 - for Saccharopolyspora cebuensisyesAgar (18.000 g);Yeast extract (4.000 g);Peptone (2.000 g);Starch maize (10.000 g);Synthetic sea solution - M00738 (1000.000 ml)
117226CIP Medium 683yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=683

culture temp

@refgrowthtypetemperaturerange
12249positivegrowth28mesophilic
32105positivegrowth15-37
33294positivegrowth30mesophilic
67770positivegrowth28mesophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
32105aerobe
117226obligate aerobe

spore formation

  • @ref: 32105
  • spore formation: yes

halophily

  • @ref: 32105
  • salt: NaCl
  • growth: positive
  • tested relation: growth
  • concentration: 2.5-12.5 %

observation

  • @ref: 67770
  • observation: quinones: MK-9(H4), MK-8(H4), MK-10(H4)

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
3210515963ribitol+carbon source
3210522599arabinose+carbon source
3210518403L-arabitol+carbon source
3210517057cellobiose+carbon source
3210528757fructose+carbon source
3210533984fucose+carbon source
3210528260galactose+carbon source
3210517234glucose+carbon source
3210517754glycerol+carbon source
3210528087glycogen+carbon source
3210517716lactose+carbon source
3210517306maltose+carbon source
3210537684mannose+carbon source
32105506227N-acetylglucosamine+carbon source
3210516634raffinose+carbon source
3210526546rhamnose+carbon source
3210533942ribose+carbon source
3210517992sucrose+carbon source
3210527082trehalose+carbon source
3210518222xylose+carbon source
11722617632nitrate-reduction
11722616301nitrite-reduction

metabolite production

  • @ref: 117226
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

enzymes

@refvalueactivityec
32105acid phosphatase+3.1.3.2
32105alkaline phosphatase+3.1.3.1
32105catalase+1.11.1.6
117226oxidase-
117226catalase+1.11.1.6
117226urease+3.5.1.5
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)+
68382leucine arylamidase+3.4.11.1
68382valine arylamidase+
68382cystine arylamidase-3.4.11.3
68382trypsin+3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase+3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase+3.2.1.20
68382beta-glucosidase+3.2.1.21
68382N-acetyl-beta-glucosaminidase+3.2.1.52
68382alpha-mannosidase+3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
117226-++++++-+-++-+-++++-

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinenthost speciesisolation date
12249marine spongeCebu, Mactan Is.PhilippinesPHLAsia
67770Philippine sponge Haliclona sp. from CebuPhilippinesPHLAsiaHaliclona sp.
117226Animal, Marine sponge, Haliclona sp.Mactan IslandPhilippinesPHLAsia2003

isolation source categories

Cat1Cat2Cat3
#Environmental#Aquatic#Marine
#Host#Invertebrates (Other)#Porifera (Sponges)

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
122491Risk group (German classification)
1172261Risk group (French classification)

Sequence information

16S sequences

  • @ref: 12249
  • description: Saccharopolyspora cebuensis strain SPE 10-1 16S ribosomal RNA gene, partial sequence
  • accession: EF030715
  • length: 1483
  • database: ena
  • NCBI tax ID: 418759

GC content

@refGC-contentmethod
1224972.6
6777072.6high performance liquid chromatography (HPLC)

External links

@ref: 12249

culture collection no.: DSM 45019, CIP 109355, SPE 10-1, JCM 18116

straininfo link

  • @ref: 82657
  • straininfo: 297490

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny18319468Saccharopolyspora cebuensis sp. nov., a novel actinomycete isolated from a Philippine sponge (Porifera).Pimentel-Elardo SM, Tiro LP, Grozdanov L, Hentschel UInt J Syst Evol Microbiol10.1099/ijs.0.64971-02008Actinomycetales/*classification/genetics/*isolation & purification/physiology, Animals, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/analysis, DNA, Ribosomal/analysis, Fatty Acids/analysis, Genes, rRNA, Genotype, Haliclona/*microbiology, Molecular Sequence Data, Phenotype, Philippines, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Species SpecificityGenetics
Phylogeny19666795Saccharopolyspora gloriosae sp. nov., an endophytic actinomycete isolated from the stem of Gloriosa superba L.Qin S, Chen HH, Klenk HP, Kim CJ, Xu LH, Li WJInt J Syst Evol Microbiol10.1099/ijs.0.015792-02009Bacterial Typing Techniques, Base Composition, China, DNA, Bacterial/analysis, DNA, Ribosomal/analysis, Fatty Acids/analysis, Genes, rRNA, Genotype, Liliaceae/*microbiology, Molecular Sequence Data, Nucleic Acid Hybridization, Phenotype, Phylogeny, Plant Stems/*microbiology, RNA, Ribosomal, 16S/genetics, Saccharopolyspora/*classification/genetics/*isolation & purification/physiology, Sequence Analysis, DNA, Species Specificity, *Trees, *Tropical ClimateGenetics
Phylogeny27604103Saccharopolyspora griseoalba sp. nov., a novel actinomycete isolated from the Dead Sea.Jiang Y, Wei X, Chen X, Jiang Y, Xue Q, Lai H, Jiang CAntonie Van Leeuwenhoek10.1007/s10482-016-0763-92016Geologic Sediments/microbiology, Molecular Typing, Oceans and Seas, Phylogeny, RNA, Bacterial, RNA, Ribosomal, 16S, Saccharopolyspora/classification/genetics/*isolation & purification/ultrastructure, Soil Microbiology, *Water MicrobiologyEnzymology

Reference

@idauthorscataloguedoi/urltitleID_cross_referencepubmed
12249Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 45019)https://www.dsmz.de/collection/catalogue/details/culture/DSM-45019
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
32105Barberan A, Caceres Velazquez H, Jones S, Fierer N.10.1128/mSphere.00237-17Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information2835028776041
33294Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/7087
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68382Automatically annotated from API zym
82657Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID297490.1StrainInfo: A central database for resolving microbial strain identifiers
117226Curators of the CIPCollection of Institut Pasteur (CIP 109355)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20109355