Strain identifier

BacDive ID: 13460

Type strain: Yes

Species: Saccharopolyspora halophila

Strain history: <- S-K Tang, Yunnan Inst. Microbiol, China

NCBI tax ID(s): 405551 (species)

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General

@ref: 12238

BacDive-ID: 13460

DSM-Number: 45007

keywords: 16S sequence, Bacteria, aerobe, spore-forming, mesophilic, Gram-positive

description: Saccharopolyspora halophila DSM 45007 is an aerobe, spore-forming, mesophilic bacterium that was isolated from salt lake.

NCBI tax id

  • NCBI tax id: 405551
  • Matching level: species

strain history

@refhistory
12238<- S.-K. Tang; YIM 90500
67770KCTC 19162 <-- S.-K. Tang YIM 90500.
67771<- S-K Tang, Yunnan Inst. Microbiol, China

doi: 10.13145/bacdive13460.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Pseudonocardiales
  • family: Pseudonocardiaceae
  • genus: Saccharopolyspora
  • species: Saccharopolyspora halophila
  • full scientific name: Saccharopolyspora halophila Tang et al. 2009

@ref: 12238

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Pseudonocardiaceae

genus: Saccharopolyspora

species: Saccharopolyspora halophila

full scientific name: Saccharopolyspora halophila Tang et al. 2009

type strain: yes

Morphology

cell morphology

@refgram stainmotility
32568positiveno
67771positive

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
12238STARCH - MINERAL SALT - AGAR (STMS) (DSMZ Medium 252)yeshttps://mediadive.dsmz.de/medium/252Name: STARCH - MINERAL SALT - AGAR (STMS) (DSMZ Medium 252; with strain-specific modifications) Composition: NaCl 150.0 g/l Agar 14.985 g/l Starch 9.99001 g/l (NH4)2SO4 1.998 g/l CaCO3 1.998 g/l K2HPO4 0.999001 g/l MgSO4 x 7 H2O 0.999001 g/l FeSO4 x 7 H2O 0.000999001 g/l MnCl2 x 4 H2O 0.000999001 g/l ZnSO4 x 7 H2O 0.000999001 g/l Distilled water
12238BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514)yeshttps://mediadive.dsmz.de/medium/514Name: BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514) Composition: NaCl 19.45 g/l MgCl2 5.9 g/l Bacto peptone 5.0 g/l Na2SO4 3.24 g/l CaCl2 1.8 g/l Yeast extract 1.0 g/l KCl 0.55 g/l NaHCO3 0.16 g/l Fe(III) citrate 0.1 g/l KBr 0.08 g/l SrCl2 0.034 g/l H3BO3 0.022 g/l Na2HPO4 0.008 g/l Na-silicate 0.004 g/l NaF 0.0024 g/l (NH4)NO3 0.0016 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
12238positivegrowth37mesophilic
20156positiveoptimum28mesophilic
32568positivegrowth28-37mesophilic
32568positiveoptimum32.5mesophilic
67770positivegrowth37mesophilic
67771positivegrowth37mesophilic

culture pH

@refabilitytypepH
32568positivegrowth07-08
32568positiveoptimum7.5

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
32568aerobe
67771aerobe

spore formation

  • @ref: 32568
  • spore formation: yes

halophily

@refsaltgrowthtested relationconcentration
32568NaClpositivegrowth10-15 %
32568NaClpositiveoptimum12.5 %

observation

  • @ref: 67770
  • observation: quinones: MK-9(H2), MK-9(H4)

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
3256816449alanine+carbon source
3256822599arabinose+carbon source
3256822653asparagine+carbon source
3256817057cellobiose+carbon source
3256828757fructose+carbon source
3256828260galactose+carbon source
3256817234glucose+carbon source
3256815428glycine+carbon source
3256827570histidine+carbon source
3256817716lactose+carbon source
3256817306maltose+carbon source
3256829864mannitol+carbon source
3256837684mannose+carbon source
3256817268myo-inositol+carbon source
3256816634raffinose+carbon source
3256826546rhamnose+carbon source
3256817992sucrose+carbon source
3256826986threonine+carbon source
3256816199urea+carbon source
3256817151xylitol+carbon source
3256818222xylose+carbon source
3256817632nitrate+reduction

enzymes

@refvalueactivityec
32568gelatinase+
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)-
68382esterase lipase (C 8)-
68382lipase (C 14)-
68382leucine arylamidase-3.4.11.1
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase-
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase-3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
20156+--------+---------

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
12238salt lakeXinjiang ProvinceChinaCHNAsia
67770Saline lakeXinjiang, north-west ChinaChinaCHNAsia
67771From saline-alkali soilXinjiang ProvinceChinaCHNAsia

isolation source categories

Cat1Cat2Cat3
#Environmental#Aquatic#Lake (large)
#Condition#Saline

Safety information

risk assessment

  • @ref: 12238
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 12238
  • description: Saccharopolyspora halophila strain YIM 90500 16S ribosomal RNA gene, partial sequence
  • accession: DQ923129
  • length: 1509
  • database: ena
  • NCBI tax ID: 405551

GC content

@refGC-contentmethod
1223866.3high performance liquid chromatography (HPLC)
3256866.3

External links

@ref: 12238

culture collection no.: DSM 45007, KCTC 19162, YIM 90500, JCM 16221

straininfo link

  • @ref: 82652
  • straininfo: 297483

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny19244440Saccharopolyspora halophila sp. nov., a novel halophilic actinomycete isolated from a saline lake in China.Tang SK, Wang Y, Cai M, Zhi XY, Lou K, Xu LH, Jiang CL, Li WJInt J Syst Evol Microbiol10.1099/ijs.0.65705-02009Actinomycetales/*classification/genetics/isolation & purification/physiology, Bacterial Typing Techniques, China, DNA, Bacterial/analysis/isolation & purification, DNA, Ribosomal/analysis, Fresh Water/chemistry/microbiology, Genes, rRNA, Molecular Sequence Data, Phenotype, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Sodium Chloride, Species SpecificityEnzymology
Phylogeny27604103Saccharopolyspora griseoalba sp. nov., a novel actinomycete isolated from the Dead Sea.Jiang Y, Wei X, Chen X, Jiang Y, Xue Q, Lai H, Jiang CAntonie Van Leeuwenhoek10.1007/s10482-016-0763-92016Geologic Sediments/microbiology, Molecular Typing, Oceans and Seas, Phylogeny, RNA, Bacterial, RNA, Ribosomal, 16S, Saccharopolyspora/classification/genetics/*isolation & purification/ultrastructure, Soil Microbiology, *Water MicrobiologyEnzymology
Phylogeny29458666Saccharopolyspora deserti sp. nov., a novel halotolerant actinobacterium isolated from a desert.Yang ZW, Salam N, Asem MD, Fang BZ, Lan L, Xiao M, Wadaan MAM, Hozzein WN, Li WJInt J Syst Evol Microbiol10.1099/ijsem.0.0025982018Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, *Desert Climate, Diaminopimelic Acid/chemistry, Fatty Acids/chemistry, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Saccharopolyspora/*classification/genetics/isolation & purification, *Salinity, Saudi Arabia, Sequence Analysis, DNA, Vitamin K 2/analogs & derivatives/chemistryTranscriptome
Phylogeny30820712Saccharopolyspora qinghaiensis sp. nov., a novel actinobacterium isolated from a salt lake.Jiang Y, Lai H, Meng C, Zheng Z, Guo Q, Xue Q, Wei XAntonie Van Leeuwenhoek10.1007/s10482-019-01237-z2019Bacterial Typing Techniques, Base Composition, China, DNA, Bacterial/genetics, Fatty Acids/chemistry/metabolism, Lakes/analysis/*microbiology, Phylogeny, RNA, Ribosomal, 16S/genetics, Saccharopolyspora/classification/genetics/*isolation & purification/metabolism, Sodium Chloride/analysis/metabolismMetabolism

Reference

@idauthorscataloguedoi/urltitleID_cross_referencepubmed
12238Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 45007)https://www.dsmz.de/collection/catalogue/details/culture/DSM-45007
20156Wink, J.http://www.dsmz.de/microorganisms/wink_pdf/DSM45007.pdfCompendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
32568Barberan A, Caceres Velazquez H, Jones S, Fierer N.10.1128/mSphere.00237-17Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information2878328776041
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
67771Curators of the KCTChttps://kctc.kribb.re.kr/En/Kctc
68382Automatically annotated from API zym
82652Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID297483.1StrainInfo: A central database for resolving microbial strain identifiers