Strain identifier

BacDive ID: 13398

Type strain: Yes

Species: Pseudonocardia ailaonensis

Strain history: <- S Qin, Yunnan Inst Microbiol, China [6316]

NCBI tax ID(s): 367279 (species)

« back to strain detail view
For citation purpose refer to the digital object identifier (doi) of the current version.
Archive
version 8.1 (current version):
version 8:
version 7.1:
version 7:
version 6:
version 5:
version 4.1:
version 4:
version 3:
version 2.1:
version 2:
version 1:
version 8.1 (current version)

General

@ref: 12138

BacDive-ID: 13398

DSM-Number: 44979

keywords: 16S sequence, Bacteria, aerobe, spore-forming, mesophilic, Gram-positive, rod-shaped

description: Pseudonocardia ailaonensis DSM 44979 is an aerobe, spore-forming, mesophilic bacterium that builds an aerial mycelium and was isolated from forest soil.

NCBI tax id

  • NCBI tax id: 367279
  • Matching level: species

strain history

@refhistory
12138<- W.-J. Li, YIM <- Y.-Y. Su
67770DSM 44979 <-- W.-J. Li YIM 45505 <-- Y.-Y. Su.
67771<- S Qin, Yunnan Inst Microbiol, China [6316]

doi: 10.13145/bacdive13398.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Pseudonocardiales
  • family: Pseudonocardiaceae
  • genus: Pseudonocardia
  • species: Pseudonocardia ailaonensis
  • full scientific name: Pseudonocardia ailaonensis Qin et al. 2008

@ref: 12138

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Pseudonocardiaceae

genus: Pseudonocardia

species: Pseudonocardia ailaonensis

full scientific name: Pseudonocardia ailaonensis Qin et al. 2008

type strain: yes

Morphology

cell morphology

@refgram staincell shapemotility
32578positiverod-shapedno
67771positive

colony morphology

@refcolony colorincubation periodmedium used
20147Broom yellow (1032)10-14 daysISP 2
20147Beige (1001)10-14 daysISP 3
20147Beige (1001)10-14 daysISP 4
20147Beige (1001)10-14 daysISP 5
20147Beige (1001)10-14 daysISP 6
20147Broom yellow (1032)10-14 daysISP 7

multicellular morphology

@refforms multicellular complexcomplex namecomplex colormedium name
20147yesAerial MyceliumCreamISP 2
20147yesAerial MyceliumCreamISP 3
20147noISP 4
20147noISP 5
20147yesAerial MyceliumCreamISP 6
20147noISP 7

pigmentation

  • @ref: 32578
  • production: no

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
12138GPHF-MEDIUM (DSMZ Medium 553)yeshttps://mediadive.dsmz.de/medium/553Name: GPHF-MEDIUM (DSMZ Medium 553) Composition: Agar 20.0 g/l Glucose 10.0 g/l Beef extract 5.0 g/l Yeast extract 5.0 g/l Casein peptone 5.0 g/l CaCl2 x 2 H2O 0.74 g/l Distilled water
12138GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)yeshttps://mediadive.dsmz.de/medium/65Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water
20147ISP 2yesName: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
20147ISP 3yesName: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
20147ISP 4yesName: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
20147ISP 5yesName: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
20147ISP 6yesName: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes
20147ISP 7yesName: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes

culture temp

@refgrowthtypetemperaturerange
12138positivegrowth28mesophilic
20147positiveoptimum28mesophilic
32578positivegrowth15-37
32578positiveoptimum28mesophilic
67770positivegrowth28mesophilic
67771positivegrowth28mesophilic

culture pH

@refabilitytypepH
32578positivegrowth06-08
32578positiveoptimum07-08

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
32578aerobe
67771aerobe

spore formation

  • @ref: 32578
  • spore formation: yes

halophily

@refsaltgrowthtested relationconcentration
32578NaClpositivegrowth0-5 %
32578NaClpositiveoptimum0-5 %

observation

@refobservation
32578aggregates in chains
67770quinones: MK-8(H4)

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
3257822599arabinose+carbon source
3257828260galactose+carbon source
3257817234glucose+carbon source
3257833942ribose+carbon source
3257817632nitrate+reduction

enzymes

@refvalueactivityec
32578catalase+1.11.1.6
32578urease+3.5.1.5
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)-
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase+
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase-
68382alpha-galactosidase+3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase+3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
20147++--++---+-+--+----

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
12138forest soilYunnan ProvinceChinaCHNAsia
67770SoilYunnan ProvinceChinaCHNAsia
67771From soilChinaCHNAsia

isolation source categories

Cat1Cat2Cat3
#Environmental#Terrestrial#Forest
#Environmental#Terrestrial#Soil

taxonmaps

  • @ref: 69479
  • File name: preview.99_178435.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_10;96_2672;97_3280;98_12070;99_178435&stattab=map
  • Last taxonomy: Pseudonocardia ailaonensis
  • 16S sequence: DQ344632
  • Sequence Identity:
  • Total samples: 72
  • soil counts: 39
  • aquatic counts: 13
  • animal counts: 8
  • plant counts: 12

Safety information

risk assessment

  • @ref: 20147
  • biosafety level: 1
  • biosafety level comment: German classification

Sequence information

16S sequences

  • @ref: 12138
  • description: Pseudonocardia ailaonensis strain YIM 45505 16S ribosomal RNA gene, partial sequence
  • accession: DQ344632
  • length: 1467
  • database: ena
  • NCBI tax ID: 367279

GC content

@refGC-contentmethod
1213874.1high performance liquid chromatography (HPLC)
3257874.1

External links

@ref: 12138

culture collection no.: DSM 44979, JCM 16009, KCTC 19315, YIM 45505

straininfo link

  • @ref: 82595
  • straininfo: 294136

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny18768609Pseudonocardia ailaonensis sp. nov., isolated from soil in China.Qin S, Su YY, Zhang YQ, Wang HB, Jiang CL, Xu LH, Li WJInt J Syst Evol Microbiol10.1099/ijs.0.65721-02008Actinomycetales/*classification/genetics/*isolation & purification/physiology, Aerobiosis, Bacterial Typing Techniques, Base Composition, China, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Genes, rRNA, Molecular Sequence Data, Nucleic Acid Hybridization, Phylogeny, Pigments, Biological/biosynthesis, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Sequence Homology, Nucleic Acid, *Soil MicrobiologyGenetics
Phylogeny22140165Pseudonocardia xishanensis sp. nov., an endophytic actinomycete isolated from the roots of Artemisia annua L.Zhao GZ, Li J, Zhu WY, Wei DQ, Zhang JL, Xu LH, Li WJInt J Syst Evol Microbiol10.1099/ijs.0.037028-02011Actinomycetales/*classification/genetics/isolation & purification, Artemisia annua/*microbiology, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Endophytes/classification/genetics/isolation & purification, Fatty Acids/analysis, Molecular Sequence Data, *Phylogeny, Plant Roots/*microbiology, Quinones/analysis, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNAEnzymology
Phylogeny33890128Pseudonocardia pini sp. nov., an endophytic actinobacterium isolated from roots of the pine tree Callitris preissii.Kaewkla O, Franco CMMArch Microbiol10.1007/s00203-021-02309-32021*Cupressaceae/microbiology, Fatty Acids/analysis, Nucleic Acid Hybridization, Phylogeny, *Plant Roots/microbiology, *Pseudonocardia/classification/genetics, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Species SpecificityTranscriptome

Reference

@idauthorscataloguedoi/urltitleID_cross_referencepubmed
12138Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 44979)https://www.dsmz.de/collection/catalogue/details/culture/DSM-44979
20147Wink, J.http://www.dsmz.de/microorganisms/wink_pdf/DSM44979.pdfCompendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
32578Barberan A, Caceres Velazquez H, Jones S, Fierer N.10.1128/mSphere.00237-17Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information2879328776041
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
67771Curators of the KCTChttps://kctc.kribb.re.kr/En/Kctc
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
82595Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID294136.1StrainInfo: A central database for resolving microbial strain identifiers