Strain identifier
BacDive ID: 133667
Type strain:
Species: Salinisphaera orenii
Strain Designation: MK-B5
Strain history: S.-J. Park MK-B5.
NCBI tax ID(s): 856731 (species)
version 9.1 (current version)
General
@ref: 30036
BacDive-ID: 133667
keywords: genome sequence, 16S sequence, Bacteria, aerobe, Gram-negative, motile, rod-shaped
description: Salinisphaera orenii MK-B5 is an aerobe, Gram-negative, motile bacterium that was isolated from Solar saltern.
NCBI tax id
- NCBI tax id: 856731
- Matching level: species
strain history
- @ref: 67770
- history: S.-J. Park MK-B5.
doi: 10.13145/bacdive133667.20240916.9.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/pseudomonadota
- domain: Bacteria
- phylum: Pseudomonadota
- class: Gammaproteobacteria
- order: Nevskiales
- family: Salinisphaeraceae
- genus: Salinisphaera
- species: Salinisphaera orenii
- full scientific name: Salinisphaera orenii Park et al. 2012
@ref: 30036
domain: Bacteria
phylum: Proteobacteria
class: Gammaproteobacteria
order: Nevskiales
family: Salinisphaeraceae
genus: Salinisphaera
species: Salinisphaera orenii
strain designation: MK-B5
type strain: yes
Morphology
cell morphology
@ref | gram stain | cell length | cell width | cell shape | motility | confidence |
---|---|---|---|---|---|---|
30036 | negative | 1.75 µm | 0.6 µm | rod-shaped | yes | |
69480 | negative | 100 |
pigmentation
- @ref: 30036
- production: yes
Culture and growth conditions
culture temp
@ref | growth | type | temperature |
---|---|---|---|
30036 | positive | growth | 10-40 |
30036 | positive | optimum | 36 |
67770 | positive | growth | 37 |
culture pH
@ref | ability | type | pH | PH range |
---|---|---|---|---|
30036 | positive | growth | 5-8.5 | alkaliphile |
30036 | positive | optimum | 7.25 |
Physiology and metabolism
oxygen tolerance
- @ref: 30036
- oxygen tolerance: aerobe
spore formation
- @ref: 30036
- spore formation: no
halophily
@ref | salt | growth | tested relation | concentration |
---|---|---|---|---|
30036 | NaCl | positive | growth | 5-25 % |
30036 | NaCl | positive | optimum | 15 % |
observation
- @ref: 67770
- observation: quinones: Q-8
metabolite utilization
@ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
---|---|---|---|---|
30036 | 58143 | 5-dehydro-D-gluconate | + | carbon source |
30036 | 22599 | arabinose | + | carbon source |
30036 | 17754 | glycerol | + | carbon source |
30036 | 506227 | N-acetylglucosamine | + | carbon source |
30036 | 18401 | phenylacetate | + | carbon source |
30036 | 33942 | ribose | + | carbon source |
enzymes
@ref | value | activity | ec |
---|---|---|---|
30036 | alkaline phosphatase | + | 3.1.3.1 |
30036 | catalase | + | 1.11.1.6 |
30036 | cytochrome oxidase | + | 1.9.3.1 |
Isolation, sampling and environmental information
isolation
@ref | sample type | country | origin.country | continent |
---|---|---|---|---|
30036 | Solar saltern | |||
67770 | Sediment of a solar saltern pond in Gomso Bay | Republic of Korea | KOR | Asia |
isolation source categories
- Cat1: #Condition
- Cat2: #Saline
taxonmaps
- @ref: 69479
- File name: preview.99_12341.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_357;96_1636;97_1958;98_9297;99_12341&stattab=map
- Last taxonomy: Salinisphaera
- 16S sequence: HM137558
- Sequence Identity:
- Total samples: 1862
- soil counts: 14
- aquatic counts: 1677
- animal counts: 163
- plant counts: 8
Sequence information
16S sequences
- @ref: 30036
- description: Salinisphaera orenii MK-B5 16S ribosomal RNA gene, partial sequence
- accession: HM137558
- length: 1421
- database: nuccore
- NCBI tax ID: 856730
Genome sequences
@ref | description | accession | assembly level | database | NCBI tax ID |
---|---|---|---|---|---|
66792 | Salinisphaera orenii MK-B5 | 856730.3 | wgs | patric | 856730 |
67770 | Salinisphaera orenii MK-B5 | GCA_003788635 | contig | ncbi | 856730 |
GC content
@ref | GC-content | method |
---|---|---|
30036 | 63.4 | |
67770 | 63.4 | thermal denaturation, midpoint method (Tm) |
Genome-based predictions
predictions
@ref | trait | model | description | prediction | confidence | training_data |
---|---|---|---|---|---|---|
69480 | gram-positive | gram-positive | Positive reaction to Gram-staining | no | 100 | yes |
69480 | anaerobic | anaerobic | Ability to grow under anoxygenic conditions (including facultative anaerobes) | no | 94.613 | no |
69480 | aerobic | aerobic | Ability to grow under oxygenic conditions (including facultative aerobes) | yes | 81.541 | no |
69480 | spore-forming | spore-forming | Ability to form endo- or exospores | no | 88.991 | yes |
69480 | thermophilic | thermophile | Ability to grow at temperatures above or equal to 45°C | no | 92.554 | no |
69480 | flagellated | motile2+ | Ability to perform flagellated movement | no | 56.828 | no |
External links
@ref: 30036
culture collection no.: KCTC 23198, JCM 17073
literature
topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
---|---|---|---|---|---|---|---|---|
Phylogeny | 21984663 | Salinisphaera orenii sp. nov., isolated from a solar saltern. | Park SJ, Cha IT, Kim SJ, Shin KS, Hong Y, Roh DH, Rhee SK | Int J Syst Evol Microbiol | 10.1099/ijs.0.028647-0 | 2011 | Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/analysis, Gammaproteobacteria/*classification/genetics/isolation & purification, Geologic Sediments/microbiology, Molecular Sequence Data, Phenotype, *Phylogeny, RNA, Ribosomal, 16S/genetics, Republic of Korea, Seawater/*microbiology, Sequence Analysis, DNA, Ubiquinone/analysis, *Water Microbiology | Genetics |
Phylogeny | 33034549 | Salifodinibacter halophilus gen. nov., sp. nov., a halophilic gammaproteobacterium in the family Salinisphaeraceae isolated from a salt mine in the Colombian Andes. | Parada-Pinilla MP, Diaz-Cardenas C, Lopez G, Diaz-Riano JI, Gonzalez LN, Restrepo S, Trujillo ME, Baena S | Int J Syst Evol Microbiol | 10.1099/ijsem.0.004490 | 2020 | Bacterial Typing Techniques, Base Composition, Colombia, DNA, Bacterial/genetics, Fatty Acids/chemistry, Gammaproteobacteria/*classification/isolation & purification, Genome Size, Geologic Sediments/microbiology, *Mining, *Phylogeny, Quinones/chemistry, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Sodium Chloride, Water Microbiology | Transcriptome |
Reference
@id | authors | title | doi/url | ID_cross_reference | pubmed |
---|---|---|---|---|---|
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | 10.1099/ijsem.0.004332 | ||
30036 | Barberan A, Caceres Velazquez H, Jones S, Fierer N. | Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information | 10.1128/mSphere.00237-17 | 26397 | 28776041 |
66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | https://diaspora-project.de/progress.html#genomes | ||
67770 | Curators of the JCM | https://jcm.brc.riken.jp/en/ | |||
69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | MicrobeAtlas 1.0 beta | https://microbeatlas.org/ | ||
69480 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | https://diaspora-project.de/progress.html#genomes |