Strain identifier

BacDive ID: 133373

Type strain: Yes

Species: Luteolibacter flavescens

Strain Designation: GKX, A1600

Strain history: <- Chongya Zhang, Zhejiang Univ.

NCBI tax ID(s): 1859460 (species)

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General

@ref: 25225

BacDive-ID: 133373

keywords: 16S sequence, Bacteria, aerobe, Gram-negative, rod-shaped, colony-forming

description: Luteolibacter flavescens GKX is an aerobe, Gram-negative, rod-shaped bacterium that forms circular colonies and was isolated from deep seawater .

NCBI tax id

  • NCBI tax id: 1859460
  • Matching level: species

strain history

  • @ref: 67771
  • history: <- Chongya Zhang, Zhejiang Univ.

doi: 10.13145/bacdive133373.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/verrucomicrobiota
  • domain: Bacteria
  • phylum: Verrucomicrobiota
  • class: Verrucomicrobiia
  • order: Verrucomicrobiales
  • family: Verrucomicrobiaceae
  • genus: Luteolibacter
  • species: Luteolibacter flavescens
  • full scientific name: Luteolibacter flavescens Zhang et al. 2017

@ref: 25225

domain: Bacteria

phylum: Verrucomicrobia

class: Verrucomicrobiae

order: Verrucomicrobiales

family: Verrucomicrobiaceae

genus: Luteolibacter

species: Luteolibacter flavescens

strain designation: GKX, A1600

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell widthcell shapemotility
25225negative0.9-1.5 µm0.5-0.7 µmrod-shapedno
67771rod-shaped
67771negative

colony morphology

  • @ref: 25225
  • colony size: 2 mm
  • colony color: yellow
  • colony shape: circular
  • incubation period: 3 days
  • medium used: modified Gause synthetic agar plate

pigmentation

  • @ref: 25225
  • production: yes
  • name: Carotenoid

Culture and growth conditions

culture medium

@refnamegrowthcomposition
25225modified Gause synthetic agar plateyessoluble starch 10 g, KNO3 1 g, K2HPO4 0.5 g, MgSO4.7H2O 0.5 g, FeSO4.7H2O 0.01 g, agar 15 g, deionized water 1000 ml, pH 7.4
25225Reasoner's 2A agar (R2A)yes
252250.3 x R2A agaryes
25225nutrient agar (NA)yes
252250.1 x TSB agaryes
25225TSB agarno
25225marine broth 2216 agarno
252250.1 x marine broth 2216 agarno
25225potato dextrose agarno
25225MacConkey agarno

culture temp

@refgrowthtypetemperaturerange
25225positivegrowth20.0-35.0
25225positiveoptimum25mesophilic
25225nogrowth37mesophilic
25225nogrowth4psychrophilic
67771positivegrowth28mesophilic

culture pH

@refabilitytypepHPH range
25225positivegrowth5.5-9.5alkaliphile
25225positiveoptimum7.5

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
25225aerobe
67771aerobe

halophily

@refsalttested relationconcentrationgrowth
25225NaCloptimum1 %
25225NaClgrowth0.0-4.0 %(w/v)positive

murein

  • @ref: 25225
  • murein short key: A31
  • type: A1gamma m-Dpm-direct

observation

  • @ref: 67771
  • observation: quinones: MK-9

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
2522517128adipate-assimilation
2522517968butyrate-assimilation
2522585146carboxymethylcellulose-hydrolysis
25225casein-hydrolysis
2522562968cellulose-hydrolysis
2522516947citrate-assimilation
2522512936D-galactose-carbon source
252255291gelatin-hydrolysis
2522517234glucose-fermentation
2522517368hypoxanthine-hydrolysis
2522518287L-fucose-carbon source
2522562345L-rhamnose-carbon source
2522517895L-tyrosine-hydrolysis
2522525115malate-assimilation
2522516301nitrite-reduction
2522518401phenylacetate-assimilation
2522532032potassium gluconate-assimilation
2522527897tryptophan-energy source
2522553424tween 20-hydrolysis
2522515318xanthine-hydrolysis
2522517925alpha-D-glucose+carbon source
2522522599arabinose+assimilation
2522517057cellobiose+carbon source
2522515824D-fructose+carbon source
2522518024D-galacturonic acid+carbon source
2522515748D-glucuronate+carbon source
2522516024D-mannose+carbon source
2522517924D-sorbitol+carbon source
2522523652dextrin+carbon source
252254853esculin+hydrolysis
2522528066gentiobiose+carbon source
2522517234glucose+assimilation
2522532323glucuronamide+carbon source
2522530849L-arabinose+carbon source
2522517716lactose+carbon source
252256359lactulose+carbon source
2522517306maltose+assimilation
2522517306maltose+carbon source
2522529864mannitol+assimilation
2522537684mannose+assimilation
2522528053melibiose+carbon source
2522537657methyl D-glucoside+carbon source
2522551850methyl pyruvate+carbon source
25225506227N-acetylglucosamine+assimilation
2522517632nitrate+reduction
2522516634raffinose+carbon source
25225skimmed milk+hydrolysis
2522528017starch+hydrolysis
2522517992sucrose+carbon source
2522527082trehalose+carbon source
2522532528turanose+carbon source
2522553423tween 40+hydrolysis
2522553423tween 40+carbon source
2522553425tween 60+hydrolysis
2522553426tween 80+hydrolysis
2522553426tween 80+carbon source
2522516199urea+hydrolysis

metabolite production

@refChebi-IDmetaboliteproduction
2522515688acetoinno
2522535581indoleno
2522516136hydrogen sulfideyes

metabolite tests

@refChebi-IDmetabolitemethylred-testvoges-proskauer-testindole test
2522517234glucose-
2522515688acetoin-
2522535581indole-

enzymes

@refvalueactivityec
25225acid phosphatase+3.1.3.2
25225alkaline phosphatase+3.1.3.1
25225alpha-chymotrypsin-3.4.21.1
25225alpha-fucosidase-3.2.1.51
25225alpha-galactosidase-3.2.1.22
25225alpha-glucosidase+3.2.1.20
25225alpha-mannosidase-3.2.1.24
25225arginine dihydrolase-3.5.3.6
25225beta-galactosidase+3.2.1.23
25225beta-glucosidase-3.2.1.21
25225beta-glucuronidase-3.2.1.31
25225catalase+1.11.1.6
25225cystine arylamidase-3.4.11.3
25225cytochrome oxidase+1.9.3.1
25225esterase (C 4)+
25225esterase Lipase (C 8)+
25225leucine arylamidase+3.4.11.1
25225lipase (C 14)-
25225N-acetyl-beta-glucosaminidase-3.2.1.52
25225naphthol-AS-BI-phosphohydrolase+
25225trypsin-3.4.21.4
25225tryptophan deaminase-4.1.99.1
25225valine arylamidase+

fatty acid profile

  • fatty acids

    @reffatty acidpercentage
    25225C15:0 anteiso5.7
    25225C14:012
    25225C14:0 3OH / C16:1 iso2
    25225C15:02.1
    25225C16:018.4
    25225C16:0 3OH3
    25225C16:1ω9c15.4
    25225C18:1ω9c1.4
    25225C18:2ω6,7c / anteiso-C18:04.3
    25225C14:0 iso30.1
    25225C14:0 iso 3OH1.5
  • type of FA analysis: whole cell analysis
  • incubation medium: R2A
  • agar/liquid: agar
  • incubation temperature: 28
  • software version: Sherlock 4.5
  • system: MIS MIDI
  • cutoff value:

Isolation, sampling and environmental information

isolation

@refsample typesampling dategeographic locationcountryorigin.countrycontinentlatitudelongitudeenrichment cultureenrichment culture compositionenrichment culture durationenrichment culture temperatureisolation procedure
25225deep seawater (depth of 2.5 km)October 2011South China SeaChinaCHNAsia19115Gause synthetic agar platesoluble starch 10 g, KNO3 1 g, K2HPO4 0.5 g, MgSO4.7H2O 0.5 g, FeSO4.7H2O 0.01 g, agar 15 g, deionized water 1000 ml, pH 7.43 days28standard dilution plating method
67771From deep sea water at the depth of 2.5kmthe South China SeaChinaCHNAsia

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Aquatic
  • Cat3: #Marine

taxonmaps

  • @ref: 69479
  • File name: preview.99_24119.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_545;96_2822;97_9951;98_18156;99_24119&stattab=map
  • Last taxonomy: Luteolibacter flavescens subclade
  • 16S sequence: KX185934
  • Sequence Identity:
  • Total samples: 425
  • soil counts: 265
  • aquatic counts: 48
  • animal counts: 57
  • plant counts: 55

Sequence information

16S sequences

  • @ref: 25225
  • description: Luteolibacter sp. GKX 16S ribosomal RNA gene, partial sequence
  • accession: KX185934
  • length: 1482
  • database: nuccore
  • NCBI tax ID: 1859460

GC content

@refGC-contentmethod
2522559.5high performance liquid chromatography (HPLC)
6777159.5

External links

@ref: 25225

culture collection no.: MCCC 1K03193, KCTC 52361

literature

  • topic: Phylogeny
  • Pubmed-ID: 27911253
  • title: Luteolibacter flavescens sp. nov., isolated from deep seawater.
  • authors: Zhang C, Dong B, Wang R, Su Y, Han S, Yu X, Zhao Z, Fu G, Sun C, Wu M
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijsem.0.001713
  • year: 2017
  • mesh: Bacterial Typing Techniques, Base Composition, China, DNA, Bacterial/genetics, Diaminopimelic Acid/chemistry, Fatty Acids/chemistry, Nucleic Acid Hybridization, Peptidoglycan/chemistry, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Seawater/*microbiology, Sequence Analysis, DNA, Verrucomicrobia/*classification/growth & development/isolation & purification, Vitamin K 2/analogs & derivatives/chemistry
  • topic2: Transcriptome

Reference

@idauthorstitledoi/urljournalpubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
25225C. D. Zhang, B.,Wang, R.,Su, Y.,Han, S.,Yu, X.,Zhao, Z.,Fu, G.,Sun, C.,Wu, M.Luteolibacter flavescens sp. nov., isolated from deep seawater10.1099/ijsem.0.001713IJSEM 67: 729-735 201727911253
67771Curators of the KCTChttps://kctc.kribb.re.kr/En/Kctc
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.MicrobeAtlas 1.0 betahttps://microbeatlas.org/