Name: Luteolibacter flavescens GKX
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 133373

Type strain:

Species: Luteolibacter flavescens

Strain Designation: GKX, A1600

Culture col. no.: MCCC 1K03193, KCTC 52361

Strain history: <- Chongya Zhang, Zhejiang Univ.

NCBI tax ID(s): 1859460 (species)

Section

Luteolibacter flavescens GKX is an aerobe, Gram-negative, rod-shaped bacterium that forms circular colonies and was isolated from deep seawater .

colony-forming
Gram-negative
rod-shaped
aerobe
16S sequence
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Verrucomicrobiota*
	Class	*Verrucomicrobiia*
	Order	*Verrucomicrobiales*
	Family	*Verrucomicrobiaceae*
	Genus	*Luteolibacter*
	Species	**Luteolibacter flavescens**
	Full Scientific Name (LPSN)	Luteolibacter flavescens Zhang et al. 2017

Information on morphological and physiological properties Morphology

[Ref.: #25225]	Gram stain	negative
[Ref.: #67771]	Gram stain	negative

[Ref.: #25225]	Cell length	0.9-1.5 µm

[Ref.: #25225]	Cell width	0.5-0.7 µm

[Ref.: #25225]	Cell shape	rod-shaped
[Ref.: #67771]	Cell shape	rod-shaped

[Ref.: #25225]	Motility	no

[Ref.: #25225]	Colony size	2 mm
[Ref.: #25225]	Colony color	yellow
[Ref.: #25225]	Colony shape	circular
[Ref.: #25225]	Incubation period	3 days
[Ref.: #25225]	Cultivation medium used	modified Gause synthetic agar plate

[Ref.: #25225]	Pigment production	yes
[Ref.: #25225]	Pigment name	Carotenoid

Information on culture and growth conditions Culture and growth conditions

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Culture medium

modified Gause synthetic agar plate

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Culture medium growth

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Culture medium composition

expand / minimize

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Culture medium

Reasoner's 2A agar (R2A)

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Culture medium growth

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Culture medium

0.3 x R2A agar

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Culture medium growth

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Culture medium

nutrient agar (NA)

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Culture medium growth

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Culture medium

0.1 x TSB agar

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Culture medium growth

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Culture medium

TSB agar

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Culture medium growth

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Culture medium

marine broth 2216 agar

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Culture medium growth

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Culture medium

0.1 x marine broth 2216 agar

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Culture medium growth

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Culture medium

potato dextrose agar

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Culture medium growth

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Culture medium

MacConkey agar

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Culture medium growth

	Kind of temperature		Temperature
[Ref.: #25225]		growth	20.0-35.0 °C
[Ref.: #25225]		optimum	25 °C
[Ref.: #25225]	no	growth	37 °C
[Ref.: #25225]	no	growth	4 °C
[Ref.: #67771]		growth	28 °C

[Ref.: #25225]	Temperature range	mesophilic
[Ref.: #25225]	Temperature range	psychrophilic
[Ref.: #67771]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #25225]			optimum	7.5
[Ref.: #25225]			growth	5.5-9.5

Information on physiology and metabolism Physiology and metabolism

[Ref.: #25225]	Oxygen tolerance	aerobe
[Ref.: #67771]	Oxygen tolerance	aerobe

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #25225]		NaCl		optimum	1	%
[Ref.: #25225]		NaCl		growth	0.0-4.0	%(w/v)

[Ref.: #25225]	Murein short key	A31
[Ref.: #25225]	Murein types	A1gamma m-Dpm-direct

[Ref.: #67771]

Observation

quinones: MK-9

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #25225]	adipate ChEBI	-	assimilation
[Ref.: #25225]	alpha-D-glucose ChEBI	+	carbon source
[Ref.: #25225]	arabinose ChEBI	+	assimilation
[Ref.: #25225]	butyrate ChEBI	-	assimilation
[Ref.: #25225]	carboxymethylcellulose ChEBI	-	hydrolysis
[Ref.: #25225]	casein	-	hydrolysis
[Ref.: #25225]	cellobiose ChEBI	+	carbon source
[Ref.: #25225]	cellulose ChEBI	-	hydrolysis
[Ref.: #25225]	citrate ChEBI	-	assimilation
[Ref.: #25225]	D-fructose ChEBI	+	carbon source
[Ref.: #25225]	D-galactose ChEBI	-	carbon source
[Ref.: #25225]	D-galacturonic acid ChEBI	+	carbon source
[Ref.: #25225]	D-glucuronate ChEBI	+	carbon source
[Ref.: #25225]	D-mannose ChEBI	+	carbon source
[Ref.: #25225]	D-sorbitol ChEBI	+	carbon source
[Ref.: #25225]	dextrin ChEBI	+	carbon source
[Ref.: #25225]	esculin ChEBI	+	hydrolysis
[Ref.: #25225]	gelatin ChEBI	-	hydrolysis
[Ref.: #25225]	gentiobiose ChEBI	+	carbon source
[Ref.: #25225]	glucose ChEBI	-	fermentation
[Ref.: #25225]	glucose ChEBI	+	assimilation
[Ref.: #25225]	glucuronamide ChEBI	+	carbon source
[Ref.: #25225]	hypoxanthine ChEBI	-	hydrolysis
[Ref.: #25225]	L-arabinose ChEBI	+	carbon source
[Ref.: #25225]	L-fucose ChEBI	-	carbon source
[Ref.: #25225]	L-rhamnose ChEBI	-	carbon source
[Ref.: #25225]	L-tyrosine ChEBI	-	hydrolysis
[Ref.: #25225]	lactose ChEBI	+	carbon source
[Ref.: #25225]	lactulose ChEBI	+	carbon source
[Ref.: #25225]	malate ChEBI	-	assimilation
[Ref.: #25225]	maltose ChEBI	+	assimilation
[Ref.: #25225]	maltose ChEBI	+	carbon source
[Ref.: #25225]	mannitol ChEBI	+	assimilation
[Ref.: #25225]	mannose ChEBI	+	assimilation
[Ref.: #25225]	melibiose ChEBI	+	carbon source
[Ref.: #25225]	methyl D-glucoside ChEBI	+	carbon source
[Ref.: #25225]	methyl pyruvate ChEBI	+	carbon source
[Ref.: #25225]	N-acetylglucosamine ChEBI	+	assimilation
[Ref.: #25225]	nitrate ChEBI	+	reduction
[Ref.: #25225]	nitrite ChEBI	-	reduction
[Ref.: #25225]	phenylacetate ChEBI	-	assimilation
[Ref.: #25225]	potassium gluconate ChEBI	-	assimilation
[Ref.: #25225]	raffinose ChEBI	+	carbon source
[Ref.: #25225]	skimmed milk	+	hydrolysis
[Ref.: #25225]	starch ChEBI	+	hydrolysis
[Ref.: #25225]	sucrose ChEBI	+	carbon source
[Ref.: #25225]	trehalose ChEBI	+	carbon source
[Ref.: #25225]	tryptophan ChEBI	-	energy source
[Ref.: #25225]	turanose ChEBI	+	carbon source
[Ref.: #25225]	tween 20 ChEBI	-	hydrolysis
[Ref.: #25225]	tween 40 ChEBI	+	hydrolysis
[Ref.: #25225]	tween 40 ChEBI	+	carbon source
[Ref.: #25225]	tween 60 ChEBI	+	hydrolysis
[Ref.: #25225]	tween 80 ChEBI	+	hydrolysis
[Ref.: #25225]	tween 80 ChEBI	+	carbon source
[Ref.: #25225]	urea ChEBI	+	hydrolysis
[Ref.: #25225]	xanthine ChEBI	-	hydrolysis
	Only first 10 entries are displayed. Click here to see all.

	Metabolite	Production
[Ref.: #25225]	acetoin ChEBI	no
[Ref.: #25225]	hydrogen sulfide ChEBI	yes
[Ref.: #25225]	indole ChEBI	no

	Metabolite	Voges-Proskauer-test	Methylred-test	Indole test
[Ref.: #25225]	acetoin ChEBI	-
[Ref.: #25225]	glucose ChEBI		-
[Ref.: #25225]	indole ChEBI			-

	Enzyme	Enzyme activity	EC number
[Ref.: #25225]	acid phosphatase	+	3.1.3.2
[Ref.: #25225]	alkaline phosphatase	+	3.1.3.1
[Ref.: #25225]	alpha-chymotrypsin	-	3.4.21.1
[Ref.: #25225]	alpha-fucosidase	-	3.2.1.51
[Ref.: #25225]	alpha-galactosidase	-	3.2.1.22
[Ref.: #25225]	alpha-glucosidase	+	3.2.1.20
[Ref.: #25225]	alpha-mannosidase	-	3.2.1.24
[Ref.: #25225]	arginine dihydrolase	-	3.5.3.6
[Ref.: #25225]	beta-galactosidase	+	3.2.1.23
[Ref.: #25225]	beta-glucosidase	-	3.2.1.21
[Ref.: #25225]	beta-glucuronidase	-	3.2.1.31
[Ref.: #25225]	catalase	+	1.11.1.6
[Ref.: #25225]	cystine arylamidase	-	3.4.11.3
[Ref.: #25225]	cytochrome oxidase	+	1.9.3.1
[Ref.: #25225]	esterase (C 4)	+
[Ref.: #25225]	esterase Lipase (C 8)	+
[Ref.: #25225]	leucine arylamidase	+	3.4.11.1
[Ref.: #25225]	lipase (C 14)	-
[Ref.: #25225]	N-acetyl-beta-glucosaminidase	-	3.2.1.52
[Ref.: #25225]	naphthol-AS-BI-phosphohydrolase	+
[Ref.: #25225]	trypsin	-	3.4.21.4
[Ref.: #25225]	tryptophan deaminase	-	4.1.99.1
[Ref.: #25225]	valine arylamidase	+
	Only first 10 entries are displayed. Click here to see all.

Fatty acid profile

Metadata FA analysis

Reference

[Ref.: #25225]

Fatty acid	Percentage
C14:0 iso	30.1
C16:0	18.4
C16:1ω9c	15.4
C14:0	12
C15:0 anteiso	5.7
C18:2ω6,7c / anteiso-C18:0	4.3
C16:0 3OH	3
C15:0	2.1
C14:0 3OH / C16:1 iso	2
C14:0 iso 3OH	1.5
C18:1ω9c	1.4

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #25225]	Sample type/isolated from	deep seawater (depth of 2.5 km)
[Ref.: #25225]	Sampling date	October 2011
[Ref.: #25225]	Geographic location (country and/or sea, region)	South China Sea
[Ref.: #25225]	Country	China
[Ref.: #25225]	Country ISO 3 Code	CHN
[Ref.: #25225]	Continent	Asia
[Ref.: #25225]	Geographic location	19.0000°/115.0000°
[Ref.: #25225]	Enrichment culture	Gause synthetic agar plate
[Ref.: #25225]	Enrichment culture composition	soluble starch 10 g, KNO3 1 g, K2HPO4 0.5 g, MgSO4.7H2O 0.5 g, FeSO4.7H2O 0.01 g, agar 15 g, deionized water 1000 ml, pH 7.4
[Ref.: #25225]	Enrichment culture duration	3 days
[Ref.: #25225]	Enrichment culture temperature	28 ̊C
[Ref.: #25225]	Isolation/enrichment procedure	standard dilution plating method

[Ref.: #67771]	Sample type/isolated from	From deep sea water at the depth of 2.5km
[Ref.: #67771]	Geographic location (country and/or sea, region)	the South China Sea
[Ref.: #67771]	Country	China
[Ref.: #67771]	Country ISO 3 Code	CHN
[Ref.: #67771]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Environmental

#Aquatic

#Marine

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence KX185934 (>99% sequence identity) for Luteolibacter flavescens subclade from Microbeatlas

Aquatic Samples	48
Soil Samples	265
Animal Samples	57
Plant Samples	55
Total Samples	425

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #25225]	Luteolibacter sp. GKX 16S ribosomal RNA gene, partial sequence	KX185934	1482	GenBank	1859460 tax ID

[Ref.: #25225]	GC-content	59.5 mol%	high performance liquid chromatography (HPLC)
[Ref.: #67771]	GC-content	59.5 mol%

Availability in culture collections External links

[Ref.: #25225]

Culture collection no.

MCCC 1K03193, KCTC 52361

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Luteolibacter flavescens sp. nov., isolated from deep seawater.	Zhang C, Dong B, Wang R, Su Y, Han S, Yu X, Zhao Z, Fu G, Sun C, Wu M	Int J Syst Evol Microbiol	10.1099/ijsem.0.001713	2017	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#25225

C. D. Zhang, B.,Wang, R.,Su, Y.,Han, S.,Yu, X.,Zhao, Z.,Fu, G.,Sun, C.,Wu, M.: Luteolibacter flavescens sp. nov., isolated from deep seawater. IJSEM 67: 729 - 735 2017 ( DOI 10.1099/ijsem.0.001713 , PubMed 27911253 )

#67771 Korean Collection for Type Cultures (KCTC) ; Curators of the KCTC;

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

* These data were automatically processed and therefore are not curated

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