Strain identifier

BacDive ID: 133323

Type strain: Yes

Species: Achromobacter aloeverae

Strain Designation: AVA-1

NCBI tax ID(s): 1750518 (species)

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General

@ref: 25141

BacDive-ID: 133323

keywords: genome sequence, 16S sequence, Bacteria, aerobe, Gram-negative, motile, rod-shaped, colony-forming

description: Achromobacter aloeverae AVA-1 is an aerobe, Gram-negative, motile bacterium that forms circular colonies and was isolated from root of Aloe vera Burm.f..

NCBI tax id

  • NCBI tax id: 1750518
  • Matching level: species

doi: 10.13145/bacdive133323.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Betaproteobacteria
  • order: Burkholderiales
  • family: Alcaligenaceae
  • genus: Achromobacter
  • species: Achromobacter aloeverae
  • full scientific name: Achromobacter aloeverae Kuncharoen et al. 2017

@ref: 25141

domain: Bacteria

phylum: Proteobacteria

class: Betaproteobacteria

order: Burkholderiales

family: Alcaligenaceae

genus: Achromobacter

species: Achromobacter aloeverae

strain designation: AVA-1

type strain: yes

Morphology

cell morphology

  • @ref: 25141
  • gram stain: negative
  • cell length: 2.5-3.0 µm
  • cell width: 0.8-1.2 µm
  • cell shape: rod-shaped
  • motility: yes
  • flagellum arrangement: peritrichous

colony morphology

  • @ref: 25141
  • colony size: 1.0-2.0 mm
  • colony color: cream
  • colony shape: circular
  • incubation period: 2 days
  • medium used: tryptic soy agar (TSA)

Culture and growth conditions

culture medium

  • @ref: 25141
  • name: tryptic soy agar (TSA)
  • growth: yes

culture temp

@refgrowthtypetemperaturerange
25141positivegrowth20.0-42.0
25141positiveoptimum30.0-37.0mesophilic
25141nogrowth45thermophilic
25141positivegrowth40thermophilic
25141positivegrowth42thermophilic

culture pH

@refabilitytypepHPH range
25141positivegrowth6.0-9.0alkaliphile
25141positiveoptimum7

Physiology and metabolism

oxygen tolerance

  • @ref: 25141
  • oxygen tolerance: aerobe

spore formation

  • @ref: 25141
  • spore formation: no

halophily

@refsaltgrowthtested relationconcentration
25141NaClpositivegrowth4 %(w/v)
25141NaClnogrowth5 %(w/v)

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
2514117128adipate-assimilation
2514129016arginine-hydrolysis
2514117634D-glucose-assimilation
2514117306maltose-assimilation
2514116899D-mannitol-assimilation
2514116024D-mannose-assimilation
2514127689decanoate-assimilation
251414853esculin-hydrolysis
251415291gelatin-hydrolysis
2514117234glucose-fermentation
2514130849L-arabinose-assimilation
2514115589L-malate-assimilation
25141506227N-acetylglucosamine-assimilation
2514117632nitrate-reduction
2514118401phenylacetate-assimilation
2514132032potassium gluconate-assimilation
2514116947citrate+assimilation
2514127897tryptophan+energy source
2514153424tween 20+hydrolysis
2514153426tween 80+hydrolysis

metabolite production

  • @ref: 25141
  • Chebi-ID: 35581
  • metabolite: indole
  • production: yes

metabolite tests

  • @ref: 25141
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: +

enzymes

@refvalueactivityec
25141acid phosphatase+3.1.3.2
25141alkaline phosphatase+3.1.3.1
25141alpha-chymotrypsin-3.4.21.1
25141alpha-fucosidase-3.2.1.51
25141alpha-galactosidase-3.2.1.22
25141alpha-glucosidase-3.2.1.20
25141alpha-mannosidase-3.2.1.24
25141beta-galactosidase-3.2.1.23
25141beta-glucosidase-3.2.1.21
25141beta-glucuronidase-3.2.1.31
25141catalase+1.11.1.6
25141cystine arylamidase-3.4.11.3
25141cytochrome oxidase+1.9.3.1
25141esterase (C 4)+
25141esterase Lipase (C 8)+
25141leucine arylamidase+3.4.11.1
25141lipase (C 14)+
25141N-acetyl-beta-glucosaminidase-3.2.1.52
25141naphthol-AS-BI-phosphohydrolase+
25141trypsin+3.4.21.4
25141tryptophan deaminase+4.1.99.1
25141urease+3.5.1.5
25141valine arylamidase+

fatty acid profile

  • fatty acids

    @reffatty acidpercentageECL
    25141C12:00.21
    25141C12:0 2OH2.54
    25141C14:00.52
    25141C14:0 3OH / C16:1 iso I7.2811
    25141C16:033.73
    25141C16:0 2OH3.53
    25141C16:0 3OH0.23
    25141C16:1ω7c / C16:1ω6c27.28
    25141C17:00.38
    25141C18:04.48
    25141C18:1ω7c / C18:1ω6c8.11
    25141cyclo C17:010.52
  • type of FA analysis: whole cell analysis
  • incubation medium: NBRC medium no. 802 (Wako Pure Chemical Industries)
  • agar/liquid: agar
  • incubation temperature: 25
  • incubation time: 1
  • software version: Sherlock 6.0
  • system: MIS MIDI

Isolation, sampling and environmental information

isolation

  • @ref: 25141
  • sample type: root of Aloe vera Burm.f.
  • geographic location: Chachoengsao Province
  • country: Thailand
  • origin.country: THA
  • continent: Asia
  • enrichment culture: Starch Casein Agar medium
  • enrichment culture composition: supplemented with 50 mg l-1 of cycloheximide and 25 mg l-1 of penicillin
  • enrichment culture duration: 7 days
  • enrichment culture temperature: 30
  • isolation procedure: surface sterilization method modified of Hallmann et al.

isolation source categories

Cat1Cat2Cat3
#Host#Plants#Herbaceous plants (Grass,Crops)
#Host Body-Site#Plant#Root (Rhizome)

Sequence information

16S sequences

  • @ref: 25141
  • description: Achromobacter aloeverae gene for 16S ribosomal RNA, partial sequence, strain: AVA-1
  • accession: LC094463
  • length: 1419
  • database: nuccore
  • NCBI tax ID: 1750518

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Achromobacter aloeverae AVA-1GCA_004121055contigncbi1750518
66792Achromobacter aloeverae AVA-12881412998draftimg1750518

GC content

  • @ref: 25141
  • GC-content: 66.5
  • method: high performance liquid chromatography (HPLC)

External links

@ref: 25141

culture collection no.: LMG 29108, NBRC 111463, PCU 352, TISTR 2383

literature

  • topic: Phylogeny
  • Pubmed-ID: 27902206
  • title: Achromobacter aloeverae sp. nov., isolated from the root of Aloe vera (L.) Burm.f.
  • authors: Kuncharoen N, Muramatsu Y, Shibata C, Kamakura Y, Nakagawa Y, Tanasupawat S
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijsem.0.001566
  • year: 2017
  • mesh: Achromobacter/*classification/genetics/isolation & purification, Aloe/*microbiology, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, Nucleic Acid Hybridization, *Phylogeny, Plant Roots/*microbiology, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Thailand, Ubiquinone/chemistry
  • topic2: Transcriptome

Reference

@idauthorstitledoi/urljournalpubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
25141N. M. Kuncharoen, Y.,Shibata, C.,Kamakura, Y.,Nakagawa, Y.,Tanasupawat, S.Achromobacter aloeverae sp. nov., isolated from the root of Aloe vera (L.) Burm.f10.1099/ijsem.0.001566IJSEM 67: 37-41 201727902206
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes