Strain identifier

BacDive ID: 133298

Type strain: Yes

Species: Bordetella pseudohinzii

Strain Designation: 8-296-03

Strain history: CIP <- 2017, NCTC

NCBI tax ID(s): 1331258 (species)

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General

@ref: 42857

BacDive-ID: 133298

DSM-Number: 106160

keywords: genome sequence, Bacteria, mesophilic, Gram-negative, motile, rod-shaped

description: Bordetella pseudohinzii 8-296-03 is a mesophilic, Gram-negative, motile bacterium that was isolated from oropharyngeal swab of a C57BL/6 laboratory mouse.

NCBI tax id

  • NCBI tax id: 1331258
  • Matching level: species

strain history

@refhistory
42857<- NCTC; NCTC 13808 <- M. Bahar and K. B. Register, USDA, Beltsville <- K. Boschert; 8-296-03
121990CIP <- 2017, NCTC

doi: 10.13145/bacdive133298.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Betaproteobacteria
  • order: Burkholderiales
  • family: Alcaligenaceae
  • genus: Bordetella
  • species: Bordetella pseudohinzii
  • full scientific name: Bordetella pseudohinzii Ivanov et al. 2016

@ref: 42857

domain: Bacteria

phylum: Proteobacteria

class: Betaproteobacteria

order: Burkholderiales

family: Alcaligenaceae

genus: Bordetella

species: Bordetella pseudohinzii

full scientific name: Bordetella pseudohinzii Ivanov et al. 2016

strain designation: 8-296-03

type strain: yes

Morphology

cell morphology

@refgram staincell shapemotility
25062negativerod-shapedyes
121990negativerod-shapedyes

colony morphology

  • @ref: 25062
  • type of hemolysis: gamma
  • colony size: 1.0-1.5 mm
  • colony color: non-pigmented,translucent
  • incubation period: 2 days
  • medium used: Bordet-Gengou agar

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
25062Bordet-Gengou agaryes
42857TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535)yeshttps://mediadive.dsmz.de/medium/535Name: TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Distilled water
42857COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)yeshttps://mediadive.dsmz.de/medium/693Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base
121990CIP Medium 6yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=6

culture temp

  • @ref: 42857
  • growth: positive
  • type: growth
  • temperature: 37
  • range: mesophilic

Physiology and metabolism

halophily

  • @ref: 25062
  • salt: NaCl
  • growth: positive
  • tested relation: growth
  • concentration: 4 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
2506217521(-)-quinic acid-assimilation
25062169971,2-propandiol-assimilation
25062286442-oxopentanoate-assimilation
25062620642,3-butanediol-assimilation
25062739183-O-methyl-D-glucose-assimilation
25062182404-hydroxy-L-proline-assimilation
25062178794-hydroxybenzoate-assimilation
25062167244-hydroxybutyrate-assimilation
25062158875-aminovaleric acid-assimilation
2506227856acetamide-assimilation
2506215688acetoin-assimilation
2506215963ribitol-assimilation
2506237690allose-assimilation
2506227613amygdalin-assimilation
2506222605arabinitol-assimilation
2506218305arbutin-assimilation
2506228860beta-D-glucuronic acid-assimilation
2506273706bromosuccinate-assimilation
2506217126carnitine-assimilation
2506217057cellobiose-assimilation
2506237397chondroitin sulfate-assimilation
2506230719citraconate-assimilation
2506213997citramalate-assimilation
2506223456cyclodextrin-assimilation
2506278697D-fructose 6-phosphate-assimilation
2506215895D-galactonic acid lactone-assimilation
2506218024D-galacturonic acid-assimilation
250628391D-gluconate-assimilation
2506217784D-glucosaminic acid-assimilation
2506217634D-glucose-assimilation
2506214314D-glucose 6-phosphate-assimilation
2506215748D-glucuronate-assimilation
2506216523D-serine-assimilation
2506216398D-threonine-assimilation
2506227689decanoate-assimilation
2506223652dextrin-assimilation
2506217113erythritol-assimilation
2506216000ethanolamine-assimilation
2506228757fructose-assimilation
2506233984fucose-assimilation
2506216537galactarate-assimilation
2506216813galactitol-assimilation
2506228260galactose-assimilation
2506216865gamma-aminobutyric acid-assimilation
250625291gelatin-assimilation
2506228066gentiobiose-assimilation
2506217234glucose-assimilation
2506229042glucose 1-phosphate-assimilation
2506232323glucuronamide-assimilation
2506217754glycerol-assimilation
2506215428glycine-assimilation
2506229805glycolate-assimilation
2506236655glyoxylate-assimilation
2506217120hexanoate-assimilation
2506217268myo-inositol-assimilation
2506215443inulin-assimilation
2506216467L-arginine-assimilation
2506217464L-galactonic acid gamma-lactone-assimilation
2506215971L-histidine-assimilation
2506215699L-homoserine-assimilation
2506217191L-isoleucine-assimilation
2506218019L-lysine-assimilation
2506216643L-methionine-assimilation
2506216414L-valine-assimilation
2506275323lactitol-assimilation
2506217716lactose-assimilation
250626359lactulose-assimilation
250626364laminarin-assimilation
2506215792malonate-assimilation
2506268428maltitol-assimilation
2506217306maltose-assimilation
2506261993maltotriose-assimilation
2506228808mannan-assimilation
2506229864mannitol-assimilation
2506237684mannose-assimilation
250626731melezitose-assimilation
2506275299melibionic acid-assimilation
2506228053melibiose-assimilation
2506274611methyl (R)-lactate-assimilation
2506255507methyl alpha-D-galactoside-assimilation
2506243943methyl alpha-D-mannoside-assimilation
2506217540methyl beta-D-galactoside-assimilation
2506274863methyl beta-D-xylopyranoside-assimilation
2506237657methyl D-glucoside-assimilation
2506263154N-acetyl-beta-D-mannosamine-assimilation
2506228037N-acetylgalactosamine-assimilation
25062506227N-acetylglucosamine-assimilation
2506235418n-acetylneuraminate-assimilation
2506217632nitrate-reduction
2506216301nitrite-reduction
2506217134octopamine-assimilation
2506230623oxalate-assimilation
2506218394palatinose-assimilation
2506217309pectin-assimilation
2506250048phenylethylamine-assimilation
2506217272propionate-assimilation
2506233951psicose-assimilation
2506217148putrescine-assimilation
2506216634raffinose-assimilation
2506226546rhamnose-assimilation
2506217814salicin-assimilation
2506230762salicylate-assimilation
2506241865sebacic acid-assimilation
2506274526sec-butylamine-assimilation
2506232488sedoheptulosan-assimilation
2506230911sorbitol-assimilation
2506227922sorbose-assimilation
2506217164stachyose-assimilation
2506217992sucrose-assimilation
2506233954tagatose-assimilation
2506227082trehalose-assimilation
2506232528turanose-assimilation
2506215760tyramine-assimilation
2506217151xylitol-assimilation
2506218222xylose-assimilation
25062309162-oxoglutarate+assimilation
2506230089acetate+assimilation
2506217968butyrate+assimilation
2506216947citrate+assimilation
2506215570D-alanine+assimilation
2506217108D-arabinose+assimilation
2506229990D-aspartate+assimilation
2506230612D-glucarate+assimilation
2506217315D-glucosamine+assimilation
2506274168D-ribono-1,4-lactone+assimilation
2506216988D-ribose+assimilation
2506265327D-xylose+assimilation
2506229806fumarate+assimilation
2506215978glycerol 3-phosphate+assimilation
2506270744glycine-proline+assimilation
2506273804glycyl L-aspartic acid+assimilation
2506221217L-alaninamide+assimilation
2506216977L-alanine+assimilation
2506273786L-alanylglycine+assimilation
2506230849L-arabinose+assimilation
2506217196L-asparagine+assimilation
2506229985L-glutamate+assimilation
2506218050L-glutamine+assimilation
2506215603L-leucine+assimilation
2506262320L-lyxose+assimilation
2506215729L-ornithine+assimilation
2506217295L-phenylalanine+assimilation
2506217203L-proline+assimilation
2506217115L-serine+assimilation
2506216857L-threonine+assimilation
2506225115malate+assimilation
2506275146monomethyl succinate+assimilation
2506273784glycyl-l-glutamate+assimilation
2506216452oxaloacetate+assimilation
2506215361pyruvate+assimilation
2506230031succinate+assimilation
25062132950tartrate+carbon source
6836925115malate+assimilation
6836917128adipate+assimilation
6836927689decanoate+assimilation
6836924265gluconate-assimilation
6836917306maltose-assimilation
6836959640N-acetylglucosamine-assimilation
6836916899D-mannitol-assimilation
6836916024D-mannose-assimilation
6836930849L-arabinose-assimilation
6836917634D-glucose-assimilation
683695291gelatin-hydrolysis
683694853esculin-hydrolysis
6836916199urea-hydrolysis
6836929016arginine-hydrolysis
6836917634D-glucose-fermentation
6836927897tryptophan-energy source
6836917632nitrate-reduction

metabolite production

  • @ref: 68369
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

  • @ref: 68369
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: -

enzymes

@refvalueactivityec
25062catalase+1.11.1.6
25062cytochrome oxidase+1.9.3.1
25062urease-3.5.1.5
68369cytochrome oxidase+1.9.3.1
68369gelatinase-
68369beta-glucosidase-3.2.1.21
68369urease-3.5.1.5
68369arginine dihydrolase-3.5.3.6

API 20NE

@refNO3TRPGLU_ FermADH ArgUREESCGELPNPGGLU_ AssimARAMNEMANNAGMALGNTCAPADIMLTCITPACOX
42857---------------++++++

Isolation, sampling and environmental information

isolation

@refsample typecountryorigin.countrycontinentisolation date
25062oropharyngeal swab of a C57BL/6 laboratory mouseUSAUSANorth America
42857oropharyngeal swab of C57BL/6 laboratory mouseUSAUSANorth America
121990Human, Oropharyngeal swabUnited States of AmericaUSANorth America2008-01-02

isolation source categories

Cat1Cat2Cat3
#Engineered#Laboratory
#Infection#Patient#Swab
#Host#Mammals#Muridae (Mouse/Rat)
#Host Body-Site#Oral cavity and airways

Safety information

risk assessment

  • @ref: 121990
  • biosafety level: 2
  • biosafety level comment: Risk group (French classification)

Sequence information

Genome sequences

  • @ref: 66792
  • description: Bordetella pseudohinzii 8-296-03
  • accession: GCA_000657795
  • assembly level: contig
  • database: ncbi
  • NCBI tax ID: 1331258

GC content

@refGC-contentmethod
2506266.6
4285766.6sequence analysis

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
motileyes86.624yes
motileyes86.624yes
flagellatedno52.048no
flagellatedno52.048no
gram-positiveno98.546no
gram-positiveno98.546no
anaerobicno99.102no
anaerobicno99.102no
halophileno70.257no
halophileno70.257no
spore-formingno95.382no
spore-formingno95.382no
glucose-utilno79.476yes
glucose-utilno79.476yes
aerobicyes88.742no
aerobicyes88.742no
thermophileno99.103yes
thermophileno99.103yes
glucose-fermentno89.986no
glucose-fermentno89.986no

External links

@ref: 42857

culture collection no.: DSM 106160, NCTC 13808, NRRL B-59942, CIP 111487

straininfo link

  • @ref: 91407
  • straininfo: 398862

literature

  • topic: Phylogeny
  • Pubmed-ID: 27707434
  • title: Identification and taxonomic characterization of Bordetella pseudohinzii sp. nov. isolated from laboratory-raised mice.
  • authors: Ivanov YV, Linz B, Register KB, Newman JD, Taylor DL, Boschert KR, Le Guyon S, Wilson EF, Brinkac LM, Sanka R, Greco SC, Klender PM, Losada L, Harvill ET
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijsem.0.001540
  • year: 2016
  • mesh: Animals, Bacterial Typing Techniques, Base Composition, Bordetella/*classification/genetics/isolation & purification, DNA, Bacterial/genetics, Fatty Acids/analysis, Humans, Mice, Mice, Inbred C57BL/*microbiology, Nucleic Acid Hybridization, *Phylogeny, Poultry, RNA, Ribosomal, 16S/genetics, Rabbits, Sequence Analysis, DNA
  • topic2: Pathogenicity

Reference

@idauthorstitledoi/urljournalpubmedcatalogue
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
25062Y. V. L. Ivanov, B.,Register, K. B.,Newman, J. D.,Taylor, D. L.,Boschert, K. R.,Le Guyon, S.,Wilson, E. F.,Brinkac, L. M.,Sanka, R.,Greco, S. C.,Klender, P. M.,Losada, L.,Harvill, E. T.Identification and taxonomic characterization of Bordetella pseudohinzii sp. nov. isolated from laboratory-raised mice10.1099/ijsem.0.001540IJSEM 66: 5452-5459 201627707434
42857Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-106160Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 106160)
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
68369Automatically annotated from API 20NE
91407Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID398862.1
121990Curators of the CIPhttps://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20111487Collection of Institut Pasteur (CIP 111487)