Strain identifier

BacDive ID: 133187

Type strain: Yes

Species: Actinoplanes subglobosus

Strain Designation: A-T 5400

Strain history: <- NBRC; NBRC 109645 <- C Suriyachadkun, BIOTEC; A-T 5400

NCBI tax ID(s): 1547892 (species)

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General

@ref: 64831

BacDive-ID: 133187

DSM-Number: 108650

keywords: 16S sequence, Bacteria, aerobe, spore-forming, mesophilic, Gram-positive

description: Actinoplanes subglobosus A-T 5400 is an aerobe, spore-forming, mesophilic bacterium that was isolated from mixed deciduous forest soil.

NCBI tax id

  • NCBI tax id: 1547892
  • Matching level: species

strain history

  • @ref: 64831
  • history: <- NBRC; NBRC 109645 <- C Suriyachadkun, BIOTEC; A-T 5400

doi: 10.13145/bacdive133187.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Micromonosporales
  • family: Micromonosporaceae
  • genus: Actinoplanes
  • species: Actinoplanes subglobosus
  • full scientific name: Actinoplanes subglobosus Ngaemthao et al. 2016

@ref: 64831

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Micromonosporaceae

genus: Actinoplanes

species: Actinoplanes subglobosus

full scientific name: Actinoplanes subglobosus Ngaemthao et al. 2016

strain designation: A-T 5400

type strain: yes

Morphology

cell morphology

  • @ref: 24978
  • gram stain: positive

colony morphology

@refcolony colormedium used
24978light orangeYeast extract-malt extract agar (ISP2)
24978light orange yellowInorganic salts-starch agar (ISP4)
24978vivid orangeGlycerol-asparagine agar (ISP5)

multimedia

  • @ref: 64831
  • multimedia content: https://www.dsmz.de/microorganisms/photos/DSM_108650.jpg
  • caption: Medium 535 28°C
  • intellectual property rights: © Leibniz-Institut DSMZ

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
24978Yeast extract-malt extract agar (ISP2)yes
24978Inorganic salts-starch agar (ISP4)yes
24978Glycerol-asparagine agar (ISP5)yes
64831GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)yeshttps://mediadive.dsmz.de/medium/65Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water
64831TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535)yeshttps://mediadive.dsmz.de/medium/535Name: TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Distilled water
64831GPHF-MEDIUM (DSMZ Medium 553)yeshttps://mediadive.dsmz.de/medium/553Name: GPHF-MEDIUM (DSMZ Medium 553) Composition: Agar 20.0 g/l Glucose 10.0 g/l Beef extract 5.0 g/l Yeast extract 5.0 g/l Casein peptone 5.0 g/l CaCl2 x 2 H2O 0.74 g/l Distilled water
64831ISP2 MEDIUM (DSMZ Medium 987)yeshttps://mediadive.dsmz.de/medium/987Name: ISP 2 MEDIUM (DSMZ Medium 987) Composition: Agar 20.0 g/l Malt extract 10.0 g/l Dextrose 4.0 g/l Yeast extract 4.0 g/l Distilled water
64831CZAPEK PEPTONE AGAR (DSMZ Medium 83)yeshttps://mediadive.dsmz.de/medium/83Name: CZAPEK PEPTONE AGAR (DSMZ Medium 83) Composition: Sucrose 30.0 g/l Agar 20.0 g/l Peptone 5.0 g/l NaNO3 3.0 g/l Yeast extract 2.0 g/l K2HPO4 1.0 g/l MgSO4 x 7 H2O 0.5 g/l KCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
24978positivegrowth37mesophilic
24978positivegrowth20.0-37.0
24978positiveoptimum25.0-30.0mesophilic
64831positivegrowth28mesophilic

culture pH

@refabilitytypepHPH range
24978positivegrowth6.0-10.0alkaliphile
24978positiveoptimum8.0-9.0

Physiology and metabolism

oxygen tolerance

  • @ref: 24978
  • oxygen tolerance: aerobe

spore formation

  • @ref: 24978
  • spore description: motile
  • type of spore: spore
  • spore formation: yes

halophily

  • @ref: 24978
  • salt: NaCl
  • tested relation: maximum
  • concentration: 1 %(w/v)

murein

  • @ref: 24978
  • murein short key: A31
  • type: A1gamma m-Dpm-direct

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
2497818333D-arabitol-assimilation
2497816988D-ribose-assimilation
2497817924D-sorbitol-assimilation
2497817151xylitol-assimilation
2497828053melibiose-assimilation
2497827922sorbose-assimilation
2497817057cellobiose+assimilation
2497815824D-fructose+assimilation
2497812936D-galactose+assimilation
2497817634D-glucose+assimilation
2497816899D-mannitol+assimilation
2497816024D-mannose+assimilation
2497865327D-xylose+assimilation
2497817754glycerol+assimilation
2497817268myo-inositol+assimilation
2497830849L-arabinose+assimilation
2497862345L-rhamnose+assimilation
2497817716lactose+assimilation
2497816634raffinose+assimilation
2497817814salicin+assimilation
2497817992sucrose+assimilation
2497827082trehalose+assimilation

enzymes

@refvalueactivityec
24978acid phosphatase+3.1.3.2
24978alkaline phosphatase+3.1.3.1
24978alpha-chymotrypsin+3.4.21.1
24978alpha-glucosidase+3.2.1.20
24978beta-galactosidase-3.2.1.23
24978beta-glucosidase+3.2.1.21
24978catalase+1.11.1.6
24978cystine arylamidase+3.4.11.3
24978esterase (C 4)+
24978esterase Lipase (C 8)-
24978leucine arylamidase+3.4.11.1
24978N-acetyl-beta-glucosaminidase-3.2.1.52
24978naphthol-AS-BI-phosphohydrolase+
24978trypsin+3.4.21.4
24978valine arylamidase+

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentenrichment cultureenrichment culture compositionenrichment culture durationenrichment culture temperature
24978mixed deciduous forest soilLamphun ProvinceThailandTHAAsiasoil extract agarsupplemented with 25 mg l-1 nalidixic acid, 50 mg l-1 cycloheximide and 1 mg l-1 terbinafine21 days28
64831mixed deciduous forest soilLamphun ProvinceThailandTHAAsia

isolation source categories

Cat1Cat2Cat3
#Environmental#Terrestrial#Forest
#Environmental#Terrestrial#Soil

Safety information

risk assessment

  • @ref: 64831
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 64831
  • description: Actinoplanes subglobosus strain A-T 5400 16S ribosomal RNA gene, partial sequence
  • accession: KM396265
  • length: 1443
  • database: ena
  • NCBI tax ID: 1547892

GC content

  • @ref: 24978
  • GC-content: 71
  • method: high performance liquid chromatography (HPLC)

External links

@ref: 64831

culture collection no.: BCC 42734, TBRC 5832, NBRC 109645, DSM 108650

straininfo link

  • @ref: 91389
  • straininfo: 397577

literature

  • topic: Phylogeny
  • Pubmed-ID: 27550419
  • title: Actinoplanes subglobosus sp. nov., isolated from mixed deciduous forest soil.
  • authors: Ngaemthao W, Chunhametha S, Suriyachadkun C
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijsem.0.001440
  • year: 2016
  • mesh: Bacterial Typing Techniques, Base Composition, Cell Wall/chemistry, DNA, Bacterial/genetics, Diaminopimelic Acid/chemistry, Fatty Acids/chemistry, *Forests, Micromonosporaceae/*classification/genetics/isolation & purification, Nucleic Acid Hybridization, Peptidoglycan/chemistry, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Soil Microbiology, Thailand, Vitamin K 2/analogs & derivatives/chemistry
  • topic2: Transcriptome

Reference

@idauthorstitledoi/urljournalpubmedcatalogue
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
24978W. C. Ngaemthao, Suwanee,Suriyachadkun, ChanwitActinoplanes subglobosus sp. nov., isolated from mixed deciduous forest soil10.1099/ijsem.0.001440IJSEM 66: 4850-4855 201627550419
64831Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-108650Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 108650)
91389Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID397577.1