Strain identifier

BacDive ID: 132918

Type strain: Yes

Species: Acinetobacter puyangensis

Strain Designation: BQ4-1

Strain history: CIP <- 2013, JCM <- 2011, Y. Li, Beijing Univ., Beijing, China: strain BQ4-1

NCBI tax ID(s): 1096779 (species)

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General

@ref: 24706

BacDive-ID: 132918

DSM-Number: 105039

keywords: 16S sequence, Bacteria, aerobe, mesophilic, Gram-negative, rod-shaped, plant pathogen

description: Acinetobacter puyangensis BQ4-1 is an aerobe, mesophilic, Gram-negative plant pathogen that was isolated from canker of Populus x euramericana; healthy part of bark .

NCBI tax id

  • NCBI tax id: 1096779
  • Matching level: species

strain history

@refhistory
24706<- JCM; JCM 18011 <- Y. Li, Chinese Academy of Forestry, Beijing, China; BQ4-1
67770Y. Li BQ4-1.
122079CIP <- 2013, JCM <- 2011, Y. Li, Beijing Univ., Beijing, China: strain BQ4-1

doi: 10.13145/bacdive132918.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Pseudomonadales
  • family: Moraxellaceae
  • genus: Acinetobacter
  • species: Acinetobacter puyangensis
  • full scientific name: Acinetobacter puyangensis Li et al. 2013

@ref: 24706

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Pseudomonadales

family: Moraxellaceae

genus: Acinetobacter

species: Acinetobacter puyangensis

full scientific name: Acinetobacter puyangensis Li et al. 2013

strain designation: BQ4-1

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell widthcell shapemotility
30910negative0.9 µm0.6 µmrod-shapedno
122079negativerod-shapedno

pigmentation

  • @ref: 30910
  • production: yes

Culture and growth conditions

culture medium

@refnamegrowthcompositionlink
36627MEDIUM 566- Reasoner's 2A agar for Flavobacterium micromatiyesDistilled water make up to (1000.000 ml);R2A agar (18.200 g)
24706CASO AGAR (MERCK 105458) (DSMZ Medium 220)yesName: CASO AGAR (Merck 105458) (DSMZ Medium 220) Composition: Agar 15.0 g/l Casein peptone 15.0 g/l NaCl 5.0 g/l Soy peptone 5.0 g/l Distilled waterhttps://mediadive.dsmz.de/medium/220
122079CIP Medium 566yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=566

culture temp

@refgrowthtypetemperaturerange
24706positivegrowth30mesophilic
30910positivegrowth10-41
30910positiveoptimum30
36627positivegrowth30
63659positivegrowth30
67770positivegrowth30mesophilic

culture pH

@refabilitytypepHPH range
30910positivegrowth05-11alkaliphile
30910positiveoptimum7

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
30910aerobe
63659aerobe

halophily

  • @ref: 30910
  • salt: NaCl
  • growth: positive
  • tested relation: growth
  • concentration: 0-3 %

observation

  • @ref: 30910
  • observation: aggregates in clumps

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
3091030089acetate+carbon source
3091016947citrate+carbon source
3091016236ethanol+carbon source
3091029987glutamate+carbon source
3091024996lactate+carbon source
3091018401phenylacetate+carbon source
3091028044phenylalanine+carbon source
6836925115malate+assimilation
6836917128adipate-assimilation
6836927689decanoate+assimilation
6836924265gluconate-assimilation
6836917306maltose-assimilation
6836959640N-acetylglucosamine-assimilation
6836916899D-mannitol-assimilation
6836916024D-mannose-assimilation
6836930849L-arabinose-assimilation
6836917634D-glucose-assimilation
683695291gelatin-hydrolysis
683694853esculin-hydrolysis
6836916199urea-hydrolysis
6836929016arginine-hydrolysis
6836917634D-glucose-fermentation
6836927897tryptophan-energy source
6836917632nitrate+reduction

metabolite production

  • @ref: 68369
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

  • @ref: 68369
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: -

enzymes

@refvalueactivityec
30910catalase+1.11.1.6
68369cytochrome oxidase-1.9.3.1
68369gelatinase-
68369beta-glucosidase-3.2.1.21
68369urease-3.5.1.5
68369arginine dihydrolase-3.5.3.6

API 20NE

@refNO3TRPGLU_ FermADH ArgUREESCGELPNPGGLU_ AssimARAMNEMANNAGMALGNTCAPADIMLTCITPACOX
24706+--------------+-+---

Isolation, sampling and environmental information

isolation

@refsample typehost speciesgeographic locationcountryorigin.countrycontinentlatitudelongitudesampling dateisolation date
24706canker of Populus x euramericana; healthy part of bark (1 cm from a lesion margin)Populus x euramericanaPuyang City, Henan ProvinceChinaCHNAsia35.8833115.083
63659Populus barkPuyangChinaCHNAsia2010
67770Healthy part of symptomatic bark of Populus x euramericana canker (1 cm from a lesion margin) collected from Puyang City, during July 2010Populus x euramericanaHenan ProvinceChinaCHNAsia
122079Healthy part of Populus x euramericana canker bark, symptomatic bark, 1 cm from margin of lesionPuyang City, Henan ProvinceChinaCHNAsia2010-07-01

isolation source categories

Cat1Cat2Cat3
#Host#Plants#Tree
#Host Body-Site#Plant#Bark

Safety information

risk assessment

@refpathogenicity plantbiosafety levelbiosafety level comment
24706yes, in single cases1Risk group (German classification)
1220791Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
30910Acinetobacter puyangensis strain BQ4-1 16S ribosomal RNA gene, partial sequenceJN6642551460nuccore1096779
24706Acinetobacter puyangensis strain BQ4-1 16S ribosomal RNA, partial sequenceNR_1095071460nuccore1096779

GC content

  • @ref: 24706
  • GC-content: 42.5-43
  • method: thermal denaturation, midpoint method (Tm)

External links

@ref: 24706

culture collection no.: DSM 105039, CFCC 10780, JCM 18011, CCUG 69709, CIP 110585

straininfo link

@refstraininfo
91195406334
91196408638

literature

  • topic: Phylogeny
  • Pubmed-ID: 23396716
  • title: Acinetobacter puyangensis sp. nov., isolated from the healthy and diseased part of Populus xeuramericana canker bark.
  • authors: Li Y, Piao CG, Ma YC, He W, Wang HM, Chang JP, Guo LM, Wang XZ, Xie SJ, Guo MW
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijs.0.047274-0
  • year: 2013
  • mesh: Acinetobacter/*classification/genetics/isolation & purification, Bacterial Typing Techniques, Base Composition, China, DNA, Bacterial/genetics, Fatty Acids/analysis, Genes, Bacterial, Molecular Sequence Data, *Phylogeny, Plant Bark/*microbiology, Populus/*microbiology, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA
  • topic2: Genetics

Reference

@idauthorstitledoi/urlcatalogueID_cross_referencepubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
24706Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-105039Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 105039)
30910Barberan A, Caceres Velazquez H, Jones S, Fierer N.Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information10.1128/mSphere.00237-172724028776041
36627Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/30382
63659Curators of the CCUGhttps://www.ccug.se/strain?id=69709Culture Collection University of Gothenburg (CCUG) (CCUG 69709)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68369Automatically annotated from API 20NE
91195Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID406334.1
91196Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID408638.1
122079Curators of the CIPhttps://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20110585Collection of Institut Pasteur (CIP 110585)