Strain identifier

BacDive ID: 13278

Type strain: Yes

Species: Amycolatopsis viridis

Strain Designation: GY115

Variant: Isotype of BacDive ID 13277

Strain history: <- J. Swezey, NRRL; NRRL B-24837 <- A. Tan

NCBI tax ID(s): 185678 (species)

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General

@ref: 18262

BacDive-ID: 13278

DSM-Number: 45806

keywords: Bacteria, mesophilic

description: Amycolatopsis viridis GY115 is a mesophilic bacterium that builds an aerial mycelium and was isolated from arid composite Australian soil.

NCBI tax id

  • NCBI tax id: 185678
  • Matching level: species

strain history

  • @ref: 18262
  • history: <- J. Swezey, NRRL; NRRL B-24837 <- A. Tan

doi: 10.13145/bacdive13278.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Pseudonocardiales
  • family: Pseudonocardiaceae
  • genus: Amycolatopsis
  • species: Amycolatopsis viridis
  • full scientific name: Amycolatopsis viridis Zucchi et al. 2012

@ref: 18262

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Pseudonocardiaceae

genus: Amycolatopsis

species: Amycolatopsis viridis

full scientific name: Amycolatopsis viridis Zucchi et al. 2012

strain designation: GY115

variant: Isotype of BacDive ID 13277

type strain: yes

Morphology

colony morphology

@refcolony colormedium used
69441Brown grey (7013), Yellow olive (6014)suter with tyrosine
69441Beige (1001), Yellow olive (6014), Black olive (6015)ISP 5
69441Beige (1001)ISP 6
69441Ivory (1014)ISP 3
69441Ivory (1014), Yellow olive (6014)suter without tyrosine
69441Ivory (1014), Yellow olive (6014), Black olive (6015)ISP 7
69441Light ivory (1015), Reed green (6013)ISP 4
69441Sand yellow (1002)ISP 2

multicellular morphology

@refforms multicellular complexcomplex namecomplex colormedium name
69441yesAerial myceliumCream (9001)ISP 2
69441yesAerial myceliumSignal white (9003), traffic grey a (7042)ISP 3
69441noAerial myceliumISP 4
69441yesAerial myceliumLight grey (7035), stone grey (7030)ISP 5
69441yesAerial myceliumGrey white (9002)ISP 6
69441yesAerial myceliumAgate grey (7038), grey white (9002)ISP 7
69441yesAerial myceliumAgate grey (7038)suter with tyrosine
69441yesAerial myceliumSilk grey (7044)suter without tyrosine

pigmentation

@refproductionname
69441noMelanin
69441nosoluble pigment

multimedia

@refmultimedia contentcaptionintellectual property rights
69441DSM_45806_image3.jpegPlates (548, ISP2, ISP3, ISP4, ISP5, ISP7)Helmholtz-Zentrum für Infektionsforschung GmbH
69441DSM_45806_image4.jpegPlates (548, ISP2, ISP3, ISP4, ISP5, ISP7)Helmholtz-Zentrum für Infektionsforschung GmbH

Culture and growth conditions

culture medium

  • @ref: 18262
  • name: BENNETT'S AGAR (DSMZ Medium 548)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/548
  • composition: Name: BENNETT'S AGAR (DSMZ Medium 548) Composition: Agar 15.0 g/l Glucose 10.0 g/l N-Z amine 2.0 g/l Yeast extract 1.0 g/l Beef extract 1.0 g/l Distilled water

culture temp

  • @ref: 18262
  • growth: positive
  • type: growth
  • temperature: 28
  • range: mesophilic

Physiology and metabolism

halophily

  • @ref: 69441
  • salt: NaCl
  • growth: positive
  • tested relation: growth
  • concentration: 0 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6944122599arabinose-growth
6944162968cellulose-growth
6944128757fructose+growth
6944117234glucose+growth
6944117268inositol-growth
6944137684mannose-growth
6944116634raffinose-growth
6944126546rhamnose+/-growth
6944117992sucrose-growth
6944118222xylose+growth
6837917632nitrate-reduction
683794853esculin-hydrolysis
6837916199urea-hydrolysis
683795291gelatin+hydrolysis
6837917634D-glucose-fermentation
6837916988D-ribose-fermentation
6837965327D-xylose-fermentation
6837916899D-mannitol-fermentation
6837917306maltose-fermentation
6837917716lactose-fermentation
6837917992sucrose-fermentation
6837928087glycogen-fermentation

enzymes

@refvalueactivityec
68379gelatinase+
68379urease-3.5.1.5
68379beta-glucosidase-3.2.1.21
68379N-acetyl-beta-glucosaminidase-3.2.1.52
68379alpha-glucosidase-3.2.1.20
68379beta-galactosidase-3.2.1.23
68379beta-glucuronidase+3.2.1.31
68379alkaline phosphatase+3.1.3.1
68379pyrrolidonyl arylamidase-3.4.19.3
68379pyrazinamidase-3.5.1.B15
68382esterase lipase (C 8)+
68382leucine arylamidase+3.4.11.1
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382alpha-galactosidase-3.2.1.22
68382alpha-glucosidase-3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase+3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API coryne

@refNITPYZPYRAPALbeta GURbeta GALalpha GLUbeta NAGESCUREGELGLURIBXYLMANMALLACSACGLYG
69441---++-----+--------

API zym

@refAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
69441+/-+/-++/-++/-+/---++/--+/-+/---+--

Isolation, sampling and environmental information

isolation

  • @ref: 18262
  • sample type: arid composite Australian soil
  • country: Australia
  • origin.country: AUS
  • continent: Australia and Oceania

isolation source categories

Cat1Cat2Cat3
#Environmental#Terrestrial#Soil
#Climate#Hot#Arid

Safety information

risk assessment

  • @ref: 18262
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

External links

@ref: 18262

culture collection no.: DSM 45806, NCIMB 14700, NRRL B-24837

straininfo link

  • @ref: 82481
  • straininfo: 398520

literature

  • topic: Phylogeny
  • Pubmed-ID: 21378137
  • title: Amycolatopsis thermophila sp. nov. and Amycolatopsis viridis sp. nov., thermophilic actinomycetes isolated from arid soil.
  • authors: Zucchi TD, Tan GYA, Goodfellow M
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijs.0.029256-0
  • year: 2011
  • mesh: Actinomycetales/*classification/genetics/*isolation & purification/physiology, Australia, Bacterial Typing Techniques, Cluster Analysis, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Molecular Sequence Data, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Soil Microbiology
  • topic2: Genetics

Reference

@idauthorscataloguedoi/urltitle
18262Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 45806)https://www.dsmz.de/collection/catalogue/details/culture/DSM-45806
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
68379Automatically annotated from API Coryne
68382Automatically annotated from API zym
69441Wink, J.https://cdn.dsmz.de/wink/DSM%2045806.pdfCompendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
82481Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID398520.1StrainInfo: A central database for resolving microbial strain identifiers