Strain identifier
BacDive ID: 132766
Type strain:
Species: Mycobacterium vaccae
Strain Designation: Schering 260, 14 120 0006
Strain history: CIP <- 1999, V. Vincent, Inst. Pasteur, Paris, France: strain 14 120 0006 <- ATCC <- R. Bönicke: strain SN 290
NCBI tax ID(s): 1223499 (strain), 1810 (species)
General
@ref: 10934
BacDive-ID: 132766
DSM-Number: 43292
keywords: genome sequence, 16S sequence, Bacteria, obligate aerobe, mesophilic, Gram-positive, human pathogen
description: Mycobacterium vaccae Schering 260 is an obligate aerobe, mesophilic, Gram-positive human pathogen that was isolated from cow's milk.
NCBI tax id
NCBI tax id | Matching level |
---|---|
1223499 | strain |
1810 | species |
strain history
@ref | history |
---|---|
10934 | <- K. Kieslich, Schering; Schering 260 <- ATCC; ATCC 15483 <- R. Bönicke; SN 920 |
67770 | M. Tsukamura 21007 <-- ATCC 15483 <-- R. Bonicke SN 920. |
123767 | CIP <- 1999, V. Vincent, Inst. Pasteur, Paris, France: strain 14 120 0006 <- ATCC <- R. Bönicke: strain SN 290 |
doi: 10.13145/bacdive132766.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/actinomycetota
- domain: Bacteria
- phylum: Actinomycetota
- class: Actinomycetes
- order: Mycobacteriales
- family: Mycobacteriaceae
- genus: Mycobacterium
- species: Mycobacterium vaccae
- full scientific name: Mycobacterium vaccae Bönicke and Juhasz 1964 (Approved Lists 1980)
synonyms
- @ref: 20215
- synonym: Mycolicibacterium vaccae
@ref: 10934
domain: Bacteria
phylum: Actinobacteria
class: Actinobacteria
order: Actinomycetales
family: Mycobacteriaceae
genus: Mycobacterium
species: Mycobacterium vaccae
full scientific name: Mycobacterium vaccae Bönicke and Juhasz 1964 emend. Nouioui et al. 2018
strain designation: Schering 260, 14 120 0006
type strain: yes
Morphology
cell morphology
@ref | gram stain | confidence | cell shape | motility |
---|---|---|---|---|
69480 | positive | 100 | ||
123767 | rod-shaped | no |
multimedia
@ref | multimedia content | caption | intellectual property rights |
---|---|---|---|
10934 | https://www.dsmz.de/microorganisms/photos/DSM_43292.jpg | Medium 645 37°C | © Leibniz-Institut DSMZ |
10934 | https://www.dsmz.de/microorganisms/photos/DSM_43292-1.jpg | © Leibniz-Institut DSMZ |
Culture and growth conditions
culture medium
@ref | name | growth | link | composition |
---|---|---|---|---|
10934 | MIDDLEBROOK MEDIUM (DSMZ Medium 645) | yes | https://mediadive.dsmz.de/medium/645 | Name: MIDDLEBROOK MEDIUM (DSMZ Medium 645) Composition: Bacto Middlebrook 7H10 agar 20.9945 g/l Glycerol Distilled water |
36254 | MEDIUM 55 - for Mycobacterium | yes | ||
10934 | TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) | yes | https://mediadive.dsmz.de/medium/92 | Name: TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Yeast extract 3.0 g/l Distilled water |
123767 | CIP Medium 55 | yes | https://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=55 |
culture temp
@ref | growth | type | temperature | range |
---|---|---|---|---|
10934 | positive | growth | 37 | mesophilic |
36254 | positive | growth | 37 | mesophilic |
67770 | positive | growth | 37 | mesophilic |
123767 | positive | growth | 22-37 | |
123767 | no | growth | 10 | psychrophilic |
123767 | no | growth | 45 | thermophilic |
Physiology and metabolism
oxygen tolerance
- @ref: 123767
- oxygen tolerance: obligate aerobe
spore formation
- @ref: 69480
- spore formation: no
- confidence: 99.955
observation
- @ref: 67770
- observation: quinones: MK-9(H2)
metabolite utilization
@ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
---|---|---|---|---|
123767 | 606565 | hippurate | - | hydrolysis |
123767 | 17632 | nitrate | + | reduction |
123767 | 16301 | nitrite | - | reduction |
123767 | 17632 | nitrate | - | respiration |
metabolite production
- @ref: 123767
- Chebi-ID: 35581
- metabolite: indole
- production: no
enzymes
@ref | value | activity | ec |
---|---|---|---|
123767 | oxidase | - | |
123767 | beta-galactosidase | - | 3.2.1.23 |
123767 | gelatinase | - | |
123767 | amylase | - | |
123767 | DNase | - | |
123767 | caseinase | - | 3.4.21.50 |
123767 | catalase | + | 1.11.1.6 |
123767 | tween esterase | + | |
123767 | gamma-glutamyltransferase | - | 2.3.2.2 |
123767 | lecithinase | - | |
123767 | lipase | - | |
123767 | protease | - | |
123767 | urease | + | 3.5.1.5 |
68382 | alkaline phosphatase | - | 3.1.3.1 |
68382 | esterase (C 4) | + | |
68382 | esterase lipase (C 8) | + | |
68382 | lipase (C 14) | - | |
68382 | leucine arylamidase | + | 3.4.11.1 |
68382 | valine arylamidase | - | |
68382 | cystine arylamidase | + | 3.4.11.3 |
68382 | trypsin | - | 3.4.21.4 |
68382 | alpha-chymotrypsin | - | 3.4.21.1 |
68382 | acid phosphatase | + | 3.1.3.2 |
68382 | naphthol-AS-BI-phosphohydrolase | + | |
68382 | alpha-galactosidase | - | 3.2.1.22 |
68382 | beta-galactosidase | - | 3.2.1.23 |
68382 | beta-glucuronidase | - | 3.2.1.31 |
68382 | alpha-glucosidase | - | 3.2.1.20 |
68382 | beta-glucosidase | - | 3.2.1.21 |
68382 | N-acetyl-beta-glucosaminidase | - | 3.2.1.52 |
68382 | alpha-mannosidase | - | 3.2.1.24 |
68382 | alpha-fucosidase | - | 3.2.1.51 |
API zym
@ref | Control | Alkaline phosphatase | Esterase | Esterase Lipase | Lipase | Leucine arylamidase | Valine arylamidase | Cystine arylamidase | Trypsin | alpha- Chymotrypsin | Acid phosphatase | Naphthol-AS-BI-phosphohydrolase | alpha- Galactosidase | beta- Galactosidase | beta- Glucuronidase | alpha- Glucosidase | beta- Glucosidase | N-acetyl-beta- glucosaminidase | alpha- Mannosidase | alpha- Fucosidase |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
123767 | - | - | + | + | - | + | - | + | - | - | + | + | - | - | - | - | - | - | - | - |
API biotype100
@ref | GLU | FRU | GAL | TRE | MNE | SBE | MEL | SAC | RAF | MTE | MAL | LAC | LTE | MbGa | MaGa | CEL | GEN | MbGu | ESC | RIB | ARA | XYL | PLE | RHA | FUC | MLZ | DARL | LARL | XLT | DUL | TAG | GLY | INO | MAN | MTL | TUR | SOR | ADO | HBG | LYX | ERY | MDG | 3MDG | SAT | MUC | LTAT | DTAT | MTAT | DMLT | LMLT | CATE | TATE | TTE | CIT | GRT | GAT | 2KG | 5KG | TRY | NAG | GNT | PAC | PAT | pOBE | QAT | GTE | mOBE | BAT | PPAT | CMT | TGE | BET | PCE | ABT | HIN | LAT | CAP | CYT | HIS | SUC | FUM | GRE | GYT | AVT | ETN | TTN | GLN | ITA | 3OBU | APT | GTT | PRO | DALA | LALA | SER | MNT | PROP | TYR | 2KT |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
123767 | + | + | - | + | + | - | - | + | - | - | - | - | - | - | - | - | - | - | - | - | + | + | + | + | - | - | - | + | + | - | - | + | - | + | - | - | - | + | - | - | + | - | - | - | - | - | - | - | + | + | - | - | - | - | - | - | - | - | - | - | + | - | + | - | + | - | - | + | - | - | - | - | - | - | - | + | - | - | - | + | + | - | - | - | - | - | - | - | + | - | - | - | - | - | - | + | + | - | - |
Isolation, sampling and environmental information
isolation
@ref | sample type | isolation date |
---|---|---|
10934 | cow's milk | |
67770 | Cow milk | |
123767 | Food, Cow, milk | 1964 |
isolation source categories
Cat1 | Cat2 | Cat3 |
---|---|---|
#Host | #Mammals | #Bovinae (Cow, Cattle) |
#Host Body Product | #Fluids | #Milk |
Safety information
risk assessment
@ref | pathogenicity human | pathogenicity animal | biosafety level | biosafety level comment |
---|---|---|---|---|
10934 | yes | yes | 2 | Risk group (German classification) |
123767 | 1 | Risk group (French classification) |
Sequence information
16S sequences
@ref | description | accession | length | database | NCBI tax ID |
---|---|---|---|---|---|
67770 | Mycobacterium vaccae 16S ribosomal RNA gene, partial sequence | AF480591 | 1439 | ena | 1810 |
67770 | M.vaccae 16S ribosomal RNA, part | X55601 | 1447 | ena | 1810 |
Genome sequences
@ref | description | accession | assembly level | database | NCBI tax ID |
---|---|---|---|---|---|
66792 | Mycobacterium vaccae 95051 | 1354275.3 | complete | patric | 1354275 |
66792 | Mycobacterium vaccae NBRC 14118 = CIP 105934 | 1223499.3 | wgs | patric | 1223499 |
66792 | Mycolicibacterium vaccae NBRC 14118 | 2731957598 | draft | img | 1223499 |
67770 | Mycolicibacterium vaccae NBRC 14118 = CIP 105934 | GCA_001552715 | contig | ncbi | 1223499 |
67770 | Mycolicibacterium vaccae 95051 | GCA_001655245 | complete | ncbi | 1354275 |
66792 | Mycolicibacterium vaccae DSM 43292 | GCA_025821205 | contig | ncbi | 1810 |
Genome-based predictions
predictions
trait | prediction | confidence | training_data |
---|---|---|---|
spore-forming | no | 77 | no |
motile | no | 91.603 | no |
gram-positive | yes | 88.378 | no |
anaerobic | no | 99.312 | no |
halophile | no | 84.757 | no |
spore-forming | no | 85.111 | no |
thermophile | no | 99.103 | yes |
glucose-util | yes | 87.774 | no |
flagellated | no | 98.524 | no |
aerobic | yes | 90.609 | no |
glucose-ferment | no | 89.22 | no |
External links
@ref: 10934
culture collection no.: DSM 43292, ATCC 15483, NCIB 9937, SN 920, JCM 6389, ATCC 23004, BCRC 17229, CCUG 21003, CECT 3019, CIP 105934, HAMBI 2276, IFO 14118, KCTC 19087, NBRC 14118, NCIMB 9937, NCTC 10916, TMC 1526, SN 290
straininfo link
- @ref: 91067
- straininfo: 36388
literature
topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
---|---|---|---|---|---|---|---|---|
Genetics | 12101288 | Comparative evaluation of Mycobacterium vaccae as a surrogate cloning host for use in the study of mycobacterial genetics. | Medeiros MA, Dellagostin OA, Armoa GRG, Degrave WM, de Mendonca-Lima L, Lopes MQ, Costa JF, Mcfadden J, McIntosh D | Microbiology (Reading) | 10.1099/00221287-148-7-1999 | 2002 | Base Sequence, Cloning, Molecular/methods, Electroporation, *Genetic Vectors, Molecular Sequence Data, Mycobacterium/classification/*genetics, Pertussis Toxin, Plasmids, Promoter Regions, Genetic, *Transformation, Bacterial, Virulence Factors, Bordetella/genetics/metabolism | Enzymology |
Pathogenicity | 15696610 | Modulation of monocyte-tumour cell interactions by Mycobacterium vaccae. | Baran J, Baj-Krzyworzeka M, Weglarczyk K, Ruggiero I, Zembala M | Cancer Immunol Immunother | 10.1007/s00262-004-0552-6 | 2004 | *Cell Communication, Cell Line, Tumor, Humans, Immunotherapy, Interleukin-10/biosynthesis, Interleukin-12/biosynthesis, Monocytes/*physiology, Mycobacterium/*physiology, Mycobacterium bovis/physiology, Neoplasms/immunology/pathology/*therapy, Tumor Necrosis Factor-alpha/biosynthesis | |
Phylogeny | 30300123 | Description of a novel species of fast growing mycobacterium: Mycobacterium kyogaense sp. nov., a scotochromogenic strain received as Mycobacterium vaccae. | Nouioui I, Brunet LR, Simpson D, Klenk HP, Goodfellow M | Int J Syst Evol Microbiol | 10.1099/ijsem.0.003039 | 2018 | Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Genes, Bacterial, Mycobacterium/*classification/genetics/isolation & purification, Nucleic Acid Hybridization, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA | Transcriptome |
Stress | 33011306 | Comparing the effects of two different strains of mycobacteria, Mycobacterium vaccae NCTC 11659 and M. vaccae ATCC 15483, on stress-resilient behaviors and lipid-immune signaling in rats. | Loupy KM, Cler KE, Marquart BM, Yifru TW, D'Angelo HM, Arnold MR, Elsayed AI, Gebert MJ, Fierer N, Fonken LK, Frank MG, Zambrano CA, Maier SF, Lowry CA | Brain Behav Immun | 10.1016/j.bbi.2020.09.030 | 2020 | Animals, Anxiety, Lipids, Male, *Mycobacteriaceae, *Mycobacterium, Rats |
Reference
@id | authors | catalogue | doi/url | title |
---|---|---|---|---|
10934 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 43292) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-43292 | |
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |
36254 | Curators of the CIP | https://brclims.pasteur.fr/brcWeb/souche/detail/1/18008 | ||
66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |
67770 | Curators of the JCM | https://jcm.brc.riken.jp/en/ | ||
68382 | Automatically annotated from API zym | |||
69480 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |
91067 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID36388.1 | StrainInfo: A central database for resolving microbial strain identifiers | |
123767 | Curators of the CIP | Collection of Institut Pasteur (CIP 105934) | https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20105934 |