Strain identifier

BacDive ID: 132766

Type strain: Yes

Species: Mycobacterium vaccae

Strain Designation: Schering 260, 14 120 0006

Strain history: CIP <- 1999, V. Vincent, Inst. Pasteur, Paris, France: strain 14 120 0006 <- ATCC <- R. Bönicke: strain SN 290

NCBI tax ID(s): 1223499 (strain), 1810 (species)

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General

@ref: 10934

BacDive-ID: 132766

DSM-Number: 43292

keywords: genome sequence, 16S sequence, Bacteria, obligate aerobe, mesophilic, Gram-positive, human pathogen

description: Mycobacterium vaccae Schering 260 is an obligate aerobe, mesophilic, Gram-positive human pathogen that was isolated from cow's milk.

NCBI tax id

NCBI tax idMatching level
1810species
1223499strain

strain history

@refhistory
10934<- K. Kieslich, Schering; Schering 260 <- ATCC; ATCC 15483 <- R. Bönicke; SN 920
67770M. Tsukamura 21007 <-- ATCC 15483 <-- R. Bonicke SN 920.
123767CIP <- 1999, V. Vincent, Inst. Pasteur, Paris, France: strain 14 120 0006 <- ATCC <- R. Bönicke: strain SN 290

doi: 10.13145/bacdive132766.20250331.9.3

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Mycobacteriales
  • family: Mycobacteriaceae
  • genus: Mycobacterium
  • species: Mycobacterium vaccae
  • full scientific name: Mycobacterium vaccae Bönicke and Juhasz 1964 (Approved Lists 1980)

  • synonyms

    • @ref: 20215
    • synonym: Mycolicibacterium vaccae

@ref: 10934

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Mycobacteriaceae

genus: Mycobacterium

species: Mycobacterium vaccae

full scientific name: Mycobacterium vaccae Bönicke and Juhasz 1964 emend. Nouioui et al. 2018

strain designation: Schering 260, 14 120 0006

type strain: yes

Morphology

cell morphology

@refgram stainconfidencecell shapemotility
125438positive90.243
125439positive96
123767rod-shapedno

multimedia

@refmultimedia contentcaptionintellectual property rights
10934https://www.dsmz.de/microorganisms/photos/DSM_43292.jpgMedium 645 37°C© Leibniz-Institut DSMZ
10934https://www.dsmz.de/microorganisms/photos/DSM_43292-1.jpg© Leibniz-Institut DSMZ

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
10934MIDDLEBROOK MEDIUM (DSMZ Medium 645)yeshttps://mediadive.dsmz.de/medium/645Name: MIDDLEBROOK MEDIUM (DSMZ Medium 645) Composition: Bacto Middlebrook 7H10 agar 20.9945 g/l Glycerol Distilled water
36254MEDIUM 55 - for Mycobacteriumyes
10934TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)yeshttps://mediadive.dsmz.de/medium/92Name: TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Yeast extract 3.0 g/l Distilled water
123767CIP Medium 55yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=55

culture temp

@refgrowthtypetemperature
10934positivegrowth37
36254positivegrowth37
67770positivegrowth37
123767positivegrowth22-37
123767nogrowth10
123767nogrowth45

Physiology and metabolism

oxygen tolerance

@refoxygen toleranceconfidence
123767obligate aerobe
125439obligate aerobe99.1

observation

  • @ref: 67770
  • observation: quinones: MK-9(H2)

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
123767606565hippurate-hydrolysis
12376717632nitrate+reduction
12376716301nitrite-reduction
12376717632nitrate-respiration

metabolite production

  • @ref: 123767
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-glucosidase-3.2.1.20
68382beta-glucosidase-3.2.1.21
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382cystine arylamidase+3.4.11.3
68382trypsin-3.4.21.4
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)+
68382alkaline phosphatase-3.1.3.1
123767oxidase-
123767beta-galactosidase-3.2.1.23
123767gelatinase-
123767amylase-
123767DNase-
123767caseinase-3.4.21.50
123767catalase+1.11.1.6
123767tween esterase+
123767gamma-glutamyltransferase-2.3.2.2
123767lecithinase-
123767lipase-
123767protease-
123767urease+3.5.1.5
68382beta-glucuronidase-3.2.1.31
68382naphthol-AS-BI-phosphohydrolase+
68382valine arylamidase-
68382esterase (C 4)+

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
123767--++-+-+--++--------

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
123767++-++--+------------++++---++--+-+---+--+-------++----------+-+-+--+-------+---++-------+------++--

Isolation, sampling and environmental information

isolation

@refsample typeisolation date
10934cow's milk
67770Cow milk
123767Food, Cow, milk1964

isolation source categories

Cat1Cat2Cat3
#Host#Mammals#Bovinae (Cow, Cattle)
#Host Body Product#Fluids#Milk

Safety information

risk assessment

@refpathogenicity humanpathogenicity animalbiosafety levelbiosafety level comment
10934yesyes2Risk group (German classification)
1237671Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
67770Mycobacterium vaccae 16S ribosomal RNA gene, partial sequenceAF4805911439nuccore1810
67770M.vaccae 16S ribosomal RNA, partX556011447nuccore1810
124043Mycolicibacterium vaccae strain CECT 3019 16S ribosomal RNA gene, partial sequence.OK538993823nuccore1810

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Mycobacterium vaccae 950511354275.3completepatric1354275
66792Mycobacterium vaccae NBRC 14118 = CIP 1059341223499.3wgspatric1223499
66792Mycolicibacterium vaccae NBRC 141182731957598draftimg1223499
67770Mycolicibacterium vaccae NBRC 14118 = CIP 105934GCA_001552715contigncbi1223499
67770Mycolicibacterium vaccae 95051GCA_001655245completencbi1354275
66792Mycolicibacterium vaccae DSM 43292GCA_025821205contigncbi1810

Genome-based predictions

predictions

@refmodeltraitdescriptionpredictionconfidencetraining_data
125439BacteriaNetoxygen_toleranceOxygenic conditions needed for growthobligate aerobe99.1
125439BacteriaNetgram_stainReaction to gram-stainingpositive96
125439BacteriaNetmotilityAbility to perform movementno71.5
125439BacteriaNetspore_formationAbility to form endo- or exosporesno61.7
125438gram-positivegram-positivePositive reaction to Gram-stainingyes90.243no
125438anaerobicanaerobicAbility to grow under anoxygenic conditions (including facultative anaerobes)no97.815yes
125438spore-formingspore-formingAbility to form endo- or exosporesno60.971no
125438aerobicaerobicAbility to grow under oxygenic conditions (including facultative aerobes)yes87.595yes
125438thermophilethermophilicAbility to grow at temperatures above or equal to 45°Cno96yes
125438motile2+flagellatedAbility to perform flagellated movementno88no

External links

@ref: 10934

culture collection no.: DSM 43292, ATCC 15483, NCIB 9937, SN 920, JCM 6389, ATCC 23004, BCRC 17229, CCUG 21003, CECT 3019, CIP 105934, HAMBI 2276, IFO 14118, KCTC 19087, NBRC 14118, NCIMB 9937, NCTC 10916, TMC 1526, SN 290

straininfo link

  • @ref: 91067
  • straininfo: 36388

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Genetics12101288Comparative evaluation of Mycobacterium vaccae as a surrogate cloning host for use in the study of mycobacterial genetics.Medeiros MA, Dellagostin OA, Armoa GRG, Degrave WM, de Mendonca-Lima L, Lopes MQ, Costa JF, Mcfadden J, McIntosh DMicrobiology (Reading)10.1099/00221287-148-7-19992002Base Sequence, Cloning, Molecular/methods, Electroporation, *Genetic Vectors, Molecular Sequence Data, Mycobacterium/classification/*genetics, Pertussis Toxin, Plasmids, Promoter Regions, Genetic, *Transformation, Bacterial, Virulence Factors, Bordetella/genetics/metabolismEnzymology
Pathogenicity15696610Modulation of monocyte-tumour cell interactions by Mycobacterium vaccae.Baran J, Baj-Krzyworzeka M, Weglarczyk K, Ruggiero I, Zembala MCancer Immunol Immunother10.1007/s00262-004-0552-62004*Cell Communication, Cell Line, Tumor, Humans, Immunotherapy, Interleukin-10/biosynthesis, Interleukin-12/biosynthesis, Monocytes/*physiology, Mycobacterium/*physiology, Mycobacterium bovis/physiology, Neoplasms/immunology/pathology/*therapy, Tumor Necrosis Factor-alpha/biosynthesis
Phylogeny30300123Description of a novel species of fast growing mycobacterium: Mycobacterium kyogaense sp. nov., a scotochromogenic strain received as Mycobacterium vaccae.Nouioui I, Brunet LR, Simpson D, Klenk HP, Goodfellow MInt J Syst Evol Microbiol10.1099/ijsem.0.0030392018Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Genes, Bacterial, Mycobacterium/*classification/genetics/isolation & purification, Nucleic Acid Hybridization, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNATranscriptome
Stress33011306Comparing the effects of two different strains of mycobacteria, Mycobacterium vaccae NCTC 11659 and M. vaccae ATCC 15483, on stress-resilient behaviors and lipid-immune signaling in rats.Loupy KM, Cler KE, Marquart BM, Yifru TW, D'Angelo HM, Arnold MR, Elsayed AI, Gebert MJ, Fierer N, Fonken LK, Frank MG, Zambrano CA, Maier SF, Lowry CABrain Behav Immun10.1016/j.bbi.2020.09.0302020Animals, Anxiety, Lipids, Male, *Mycobacteriaceae, *Mycobacterium, Rats

Reference

@idauthorscataloguedoi/urltitle
10934Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 43292)https://www.dsmz.de/collection/catalogue/details/culture/DSM-43292
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
36254Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/18008
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68382Automatically annotated from API zym
91067Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID36388.1StrainInfo: A central database for resolving microbial strain identifiers
123767Curators of the CIPCollection of Institut Pasteur (CIP 105934)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20105934
124043Isabel Schober, Julia KoblitzData extracted from sequence databases, automatically matched based on designation and taxonomy
125438Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann10.1101/2024.08.12.607695Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets
125439Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardyhttps://github.com/GenomeNet/deepGdeepG: Deep Learning for Genome Sequence Data. R package version 0.3.1