Name: Mycobacterium vaccae Schering 260
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 132766

Type strain:

Species: Mycobacterium vaccae

Strain Designation: Schering 260, 14 120 0006

Culture col. no.: DSM 43292, ATCC 15483, NCIB 9937, SN 920, JCM 6389, ATCC 23004, BCRC 17229, CCUG 21003, CECT 3019, CIP 105934, HAMBI 2276, IFO 14118, KCTC 19087, NBRC 14118, NCIMB 9937, NCTC 10916, TMC 1526, SN 290

Strain history: CIP <- 1999, V. Vincent, Inst. Pasteur, Paris, France: strain 14 120 0006 <- ATCC <- R. Bönicke: strain SN 290

NCBI tax ID(s): 1223499 (strain), 1810 (species)

SI-ID 36388

ATCC 15483, ATCC 23004, CCUG 21003, NCTC 10916, CCUG 32166, CIP 105934, DSM 43292, NCIB 9937, JCM 6389, NCIMB 9937, IFO 14118, CECT 3019, NBRC 14118, HAMBI 2276, BCRC 17229, CCRC 17229, KCTC 19087, CNCTC 7302

Other strains from Mycobacterium vaccae

M. vaccae Gerda 21, DSM 43229, SN 903
M. vaccae Angelika 59a, DSM 43514, IMET 10670, ...
M. vaccae Angelika 67, DSM 43515, IMET 10671, ...
M. vaccae Lore 90, DSM 43516, SN 918
M. vaccae STI07433(IMET), 4465, 80004, GdfM293, ...
M. vaccae STI07434(IMET), 7972, 80008, GdfM294, ...
M. vaccae 141200001, CIP 105386
M. vaccae CIP 105695

Section

Mycobacterium vaccae Schering 260 is an obligate aerobe, mesophilic, Gram-positive human pathogen that was isolated from cow's milk.

Gram-positive
human pathogen
mesophilic
obligate aerobe
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Actinomycetota*
	Class	*Actinomycetes*
	Order	*Mycobacteriales*
	Family	*Mycobacteriaceae*
	Genus	*Mycobacterium*
	Species	**Mycobacterium vaccae**
	Full Scientific Name (LPSN)	Mycobacterium vaccae Bönicke and Juhasz 1964 (Approved Lists 1980)

Synonym

Mycolicibacterium vaccae

Information on morphological and physiological properties Morphology

[Ref.: #69480]	Gram stain	positive Confidence in %100

[Ref.: #123767]	Cell shape	rod-shaped

[Ref.: #123767]	Motility	no

Multimedia content

[Ref.: #10934]

Caption:

Medium 645 37°C

[Ref.: #10934]

Intellectual property rights:

[Ref.: #10934]

Intellectual property rights:

Information on culture and growth conditions Culture and growth conditions

[Ref.: #10934]

Culture medium

MIDDLEBROOK MEDIUM (DSMZ Medium 645)

[Ref.: #10934]

Culture medium growth

[Ref.: #10934]

Culture medium link

Medium recipe at MediaDive

[Ref.: #10934]

Culture medium composition

expand / minimize

[Ref.: #36254]

Culture medium

MEDIUM 55 - for Mycobacterium

[Ref.: #36254]

Culture medium growth

[Ref.: #10934]

Culture medium

TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)

[Ref.: #10934]

Culture medium growth

[Ref.: #10934]

Culture medium link

Medium recipe at MediaDive

[Ref.: #10934]

Culture medium composition

expand / minimize

[Ref.: #123767]

Culture medium

CIP Medium 55

[Ref.: #123767]

Culture medium growth

[Ref.: #123767]

Culture medium link

Medium recipe provided by DSMZ

	Kind of temperature		Temperature
[Ref.: #10934]		growth	37 °C
[Ref.: #36254]		growth	37 °C
[Ref.: #67770]		growth	37 °C
[Ref.: #123767]		growth	22-37 °C
[Ref.: #123767]	no	growth	10 °C
[Ref.: #123767]	no	growth	45 °C

[Ref.: #10934]	Temperature range	mesophilic
[Ref.: #36254]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic
[Ref.: #123767]	Temperature range	psychrophilic
[Ref.: #123767]	Temperature range	thermophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #123767]

Oxygen tolerance

obligate aerobe

[Ref.: #69480]

Ability of spore formation

no Confidence in %99.955

[Ref.: #67770]

Observation

quinones: MK-9(H₂)

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #123767]	hippurate ChEBI	-	hydrolysis
[Ref.: #123767]	nitrate ChEBI	+	reduction
[Ref.: #123767]	nitrate ChEBI	-	respiration
[Ref.: #123767]	nitrite ChEBI	-	reduction

	Metabolite production	Metabolite	Production
[Ref.: #123767]		indole ChEBI	no

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #68382]	alkaline phosphatase	-	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	-	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #123767]	amylase	-
[Ref.: #123767]	beta-galactosidase	-	3.2.1.23
[Ref.: #68382]	beta-galactosidase	-	3.2.1.23	* from API zym
[Ref.: #68382]	beta-glucosidase	-	3.2.1.21	* from API zym
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #123767]	caseinase	-	3.4.21.50
[Ref.: #123767]	catalase	+	1.11.1.6
[Ref.: #68382]	cystine arylamidase	+	3.4.11.3	* from API zym
[Ref.: #123767]	DNase	-
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	+		* from API zym
[Ref.: #123767]	gamma-glutamyltransferase	-	2.3.2.2
[Ref.: #123767]	gelatinase	-
[Ref.: #123767]	lecithinase	-
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #123767]	lipase	-
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #123767]	oxidase	-
[Ref.: #123767]	protease	-
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #123767]	tween esterase	+
[Ref.: #123767]	urease	+	3.5.1.5
[Ref.: #68382]	valine arylamidase	-		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® Biotype100:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	ControlQ	D-glucose as carbon sourceGLU	D-fructose as carbon sourceFRU	D-galactose as carbon sourceGAL	D-trehalose as carbon sourceTRE	D-mannose as carbon sourceMNE	L-sorbose as carbon sourceSBE	D-melibiose as carbon sourceMEL	sucrose as carbon sourceSAC	D-raffinose as carbon sourceRAF	maltotriose as carbon sourceMTE	maltose as carbon sourceMAL	lactose as carbon sourceLAC	lactulose as carbon sourceLTE	1-O-methyl-beta-galactopyranoside as carbon sourceMbGa	1-O-methyl-alpha-galactopyranoside as carbon sourceMaGa	D-cellobiose as carbon sourceCEL	gentiobiose as carbon sourceGEN	1-O-methyl-beta-D-glucopyranoside as carbon sourceMbGu	esculin as carbon sourceESC	D-ribose as carbon sourceRIB	L-arabinose as carbon sourceARA	D-xylose as carbon sourceXYL	palatinose as carbon sourcePLE	L-rhamnose as carbon sourceRHA	L-fucose as carbon sourceFUC	D-melezitose as carbon sourceMLZ	D-arabitol as carbon sourceDARL	L-arabitol as carbon sourceLARL	xylitol as carbon sourceXLT	dulcitol as carbon sourceDUL	D-tagatose as carbon sourceTAG	glycerol as carbon sourceGLY	myo-inositol as carbon sourceINO	D-mannitol as carbon sourceMAN	maltitol as carbon sourceMTL	D-turanose as carbon sourceTUR	D-sorbitol as carbon sourceSOR	adonitol as carbon sourceADO	hydroxyquinoline-beta-glucuronide as carbon sourceHBG	D-lyxose as carbon sourceLYX	i-erythritol as carbon sourceERY	1-O-methyl-alpha-D-glucoside as carbon sourceMDG	3-O-methyl-D-glucose as carbon source3MDG	D-saccharate as carbon sourceSAT	mucate as carbon sourceMUC	L-tartrate as carbon sourceLTAT	D-tartrate as carbon sourceDTAT	meso-tartrate as carbon sourceMTAT	D-malate as carbon sourceDMLT	L-malate as carbon sourceLMLT	cis-aconitate as carbon sourceCATE	trans-aconitate as carbon sourceTATE	tricarballylate as carbon sourceTTE	citrate as carbon sourceCIT	D-glucuronate as carbon sourceGRT	D-galacturonate as carbon sourceGAT	2-ketogluconate as carbon source2KG	5-ketogluconate as carbon source5KG	tryptophan as carbon sourceTRY	N-acetyl-D-glucosamine as carbon sourceNAG	D-gluconate as carbon sourceGNT	phenylacetate as carbon sourcePAC	protocatechuate as carbon sourcePAT	4-hydroxybenzoate as carbon sourcepOBE	quinate as carbon sourceQAT	gentisate as carbon sourceGTE	3-hydroxybenzoate as carbon sourcemOBE	benzoate as carbon sourceBAT	3-phenylpropionate as carbon sourcePPAT	m-coumarate as carbon sourceCMT	trigonelline as carbon sourceTGE	betaine as carbon sourceBET	putrescine as carbon sourcePCE	4-aminobutyrate as carbon sourceABT	histamine as carbon sourceHIN	DL-lactate as carbon sourceLAT	caprate as carbon sourceCAP	caprylate as carbon sourceCYT	L-histidine as carbon sourceHIS	succinate as carbon sourceSUC	fumarate as carbon sourceFUM	glutarate as carbon sourceGRE	DL-glycerate as carbon sourceGYT	5-aminovalerate as carbon sourceAVT	ethanolamine as carbon sourceETN	tryptamine as carbon sourceTTN	D-glucosamine as carbon sourceGLN	itaconate as carbon sourceITA	3-hydroxybutyrate as carbon source3OBU	L-aspartate as carbon sourceAPT	L-glutamate as carbon sourceGTT	L-proline as carbon sourcePRO	D-alanine as carbon sourceDALA	L-alanine as carbon sourceLALA	L-serine as carbon sourceSER	malonate as carbon sourceMNT	propionate as carbon sourcePROP	L-tyrosine as carbon sourceTYR	2-ketoglutarate as carbon source2KT
[Ref.: #123767]	1432	not determinedn.d.	+	+	-	+	+	-	-	+	-	-	-	-	-	-	-	-	-	-	-	-	+	+	+	+	-	-	-	+	+	-	-	+	-	+	-	-	-	+	-	-	+	-	-	-	-	-	-	-	+	+	-	-	-	-	-	-	-	-	-	-	+	-	+	-	+	-	-	+	-	-	-	-	-	-	-	+	-	-	-	+	+	-	-	-	-	-	-	-	+	-	-	-	-	-	-	+	+	-	-

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #123767]	9105	-	-	+	+	-	+	-	+	-	-	+	+	-	-	-	-	-	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #10934]	Sample type/isolated from	cow's milk

[Ref.: #67770]	Sample type/isolated from	Cow milk

[Ref.: #123767]	Sample type/isolated from	Food, Cow, milk
[Ref.: #123767]	Isolation date	1964
* marker position based on {}

Isolation sources categories

#Host	#Mammals	#Bovinae (Cow, Cattle)
#Host Body Product	#Fluids	#Milk

What are isolation sources categories?

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #10934]	Pathogenicity (human)	yes
[Ref.: #10934]	Pathogenicity (animal)	yes
[Ref.: #10934]	Biosafety level	2	Risk group (German classification) According to TRBA 466
[Ref.: #123767]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Mycobacterium vaccae 16S ribosomal RNA gene, partial sequence	AF480591	1439	ENA	1810 tax ID
[Ref.: #67770]	M.vaccae 16S ribosomal RNA, part	X55601	1447	ENA	1810 tax ID

Genome sequence information:

Only first 5 entries are displayed. Click here to see all.

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Mycolicibacterium vaccae NBRC 14118 = CIP 105934	GCA_001552715	contig	GenBank	1223499 tax ID
[Ref.: #67770]	Mycolicibacterium vaccae 95051	GCA_001655245	complete	GenBank	1354275 tax ID
[Ref.: #66792]	Mycobacterium vaccae 95051	1354275.3	complete	PATRIC	1354275 tax ID	*
[Ref.: #66792]	Mycobacterium vaccae NBRC 14118 = CIP 105934	1223499.3	wgs	PATRIC	1223499 tax ID	*
[Ref.: #66792]	Mycolicibacterium vaccae NBRC 14118	2731957598	draft	JGI IMG/M	1223499 tax ID	*
[Ref.: #66792]	Mycolicibacterium vaccae DSM 43292	GCA_025821205	contig	GenBank	1810 tax ID	*

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Mycobacterium vaccae 95051 PATRIC: 1354275.3
[Ref.: #69481]	spore-forming	no	77	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Mycobacterium vaccae 95051 PATRIC: 1354275.3
[Ref.: #69480]	motile	no	91.603	no
[Ref.: #69480]	gram-positive	yes	88.378	no
[Ref.: #69480]	anaerobic	no	99.312	no
[Ref.: #69480]	halophile	no	84.757	no
[Ref.: #69480]	spore-forming	no	85.111	no
[Ref.: #69480]	thermophile	no	99.103	yes
[Ref.: #69480]	glucose-util	yes	87.774	no
[Ref.: #69480]	flagellated	no	98.524	no
[Ref.: #69480]	aerobic	yes	90.609	no
[Ref.: #69480]	glucose-ferment	no	89.22	no

Availability in culture collections External links

[Ref.: #10934]

Culture collection no.

DSM 43292, ATCC 15483, NCIB 9937, SN 920, JCM 6389, ATCC 23004, BCRC 17229, CCUG 21003, CECT 3019, CIP 105934, HAMBI 2276, IFO 14118, KCTC 19087, NBRC 14118, NCIMB 9937, NCTC 10916, TMC 1526, SN 290

[Ref.: #91067]

SI-ID 36388

Literature:

Topic	Title	Authors	Journal	DOI	Year
Stress	Comparing the effects of two different strains of mycobacteria, Mycobacterium vaccae NCTC 11659 and M. vaccae ATCC 15483, on stress-resilient behaviors and lipid-immune signaling in rats.	Loupy KM, Cler KE, Marquart BM, Yifru TW, D'Angelo HM, Arnold MR, Elsayed AI, Gebert MJ, Fierer N, Fonken LK, Frank MG, Zambrano CA, Maier SF, Lowry CA	Brain Behav Immun	10.1016/j.bbi.2020.09.030	2020	*
Phylogeny	Description of a novel species of fast growing mycobacterium: Mycobacterium kyogaense sp. nov., a scotochromogenic strain received as Mycobacterium vaccae.	Nouioui I, Brunet LR, Simpson D, Klenk HP, Goodfellow M	Int J Syst Evol Microbiol	10.1099/ijsem.0.003039	2018	*
Pathogenicity	Modulation of monocyte-tumour cell interactions by Mycobacterium vaccae.	Baran J, Baj-Krzyworzeka M, Weglarczyk K, Ruggiero I, Zembala M	Cancer Immunol Immunother	10.1007/s00262-004-0552-6	2004	*
Genetics	Comparative evaluation of Mycobacterium vaccae as a surrogate cloning host for use in the study of mycobacterial genetics.	Medeiros MA, Dellagostin OA, Armoa GRG, Degrave WM, de Mendonca-Lima L, Lopes MQ, Costa JF, Mcfadden J, McIntosh D	Microbiology (Reading)	10.1099/00221287-148-7-1999	2002	*

References

#10934

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 43292

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#36254 ; Curators of the CIP;

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68382

Automatically annotated from API zym .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#91067

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID36388.1 )

#123767 Collection of Institut Pasteur ; Curators of the CIP; CIP 105934
* These data were automatically processed and therefore are not curated

Change proposal

You found an error in BacDive? Please tell us about it!

Note that changes will be reviewed and judged. If your changes are legitimate, changes will occur within the next BacDive update. Only proposed changes supported by the according reference will be reviewed. The BacDive team reserves the right to reject proposed changes.

Search for species Mycobacterium vaccae in external resources:
SILVA
BRENDA
PANGAEA
StrainInfo
GBIF

Mycobacterium vaccae 95051

Mycobacterium vaccae 95051

Change proposal

Change proposal