Strain identifier
BacDive ID: 132728
Type strain: 
Species: Desulfurococcus amylolyticus
Strain history: DSM 3822 <-- E. A. Bonch-Osmolovskaya Z-533.
NCBI tax ID(s): 1150674 (strain), 94694 (species)
version 8.1 (current version)
General
@ref: 24518
BacDive-ID: 132728
DSM-Number: 3822
keywords: genome sequence, 16S sequence, Archaea, hyperthermophilic
description: Desulfurococcus amylolyticus DSM 3822 is a hyperthermophilic archaeon that was isolated from hot spring.
NCBI tax id
| NCBI tax id | Matching level |
|---|---|
| 94694 | species |
| 1150674 | strain |
strain history
| @ref | history |
|---|---|
| 24518 | <- E. Bonch-Osmolovskaya, Z-533 |
| 67770 | DSM 3822 <-- E. A. Bonch-Osmolovskaya Z-533. |
doi: 10.13145/bacdive132728.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/archaea
- keyword: phylum/thermoproteota
- domain: Archaea
- phylum: Thermoproteota
- class: Thermoprotei
- order: Desulfurococcales
- family: Desulfurococcaceae
- genus: Desulfurococcus
- species: Desulfurococcus amylolyticus
- full scientific name: Desulfurococcus amylolyticus Bonch-Osmolovskaya et al. 2001
synonyms
@ref synonym 20215 Desulfurococcus fermentans 20215 Desulfurococcus kamchatkensis
@ref: 24518
domain: Archaea
phylum: Crenarchaeota
class: Thermoprotei
order: Desulfurococcales
family: Desulfurococcaceae
genus: Desulfurococcus
species: Desulfurococcus amylolyticus
full scientific name: Desulfurococcus amylolyticus Bonch-Osmolovskaya et al. 2001 emend. Perevalova et al. 2016
type strain: yes
Culture and growth conditions
culture medium
- @ref: 24518
- name: DESULFUROCOCCUS MEDIUM (DSMZ Medium 395)
- growth: yes
- link: https://mediadive.dsmz.de/medium/395
- composition: Name: DESULFUROCOCCUS MEDIUM (DSMZ Medium 395) Composition: Sulfur 9.98004 g/l D-Glucose 2.49501 g/l MgCl2 x 6 H2O 0.698603 g/l Na2S x 9 H2O 0.499002 g/l NaCl 0.499002 g/l CaCl2 x 2 H2O 0.439122 g/l KCl 0.329341 g/l KH2PO4 0.329341 g/l NH4Cl 0.329341 g/l Yeast extract 0.199601 g/l HCl 0.00249501 g/l FeCl2 x 4 H2O 0.00149701 g/l Sodium resazurin 0.000499002 g/l CoCl2 x 6 H2O 0.000189621 g/l Pyridoxine hydrochloride 9.98004e-05 g/l MnCl2 x 4 H2O 9.98004e-05 g/l ZnCl2 6.98603e-05 g/l (DL)-alpha-Lipoic acid 4.99002e-05 g/l Calcium D-(+)-pantothenate 4.99002e-05 g/l p-Aminobenzoic acid 4.99002e-05 g/l Nicotinic acid 4.99002e-05 g/l Riboflavin 4.99002e-05 g/l Thiamine HCl 4.99002e-05 g/l Na2MoO4 x 2 H2O 3.59281e-05 g/l NiCl2 x 6 H2O 2.39521e-05 g/l Folic acid 1.99601e-05 g/l Biotin 1.99601e-05 g/l H3BO3 5.98802e-06 g/l CuCl2 x 2 H2O 1.99601e-06 g/l Vitamin B12 9.98004e-07 g/l Distilled water
culture temp
| @ref | growth | type | temperature | range |
|---|---|---|---|---|
| 24518 | positive | growth | 90 | hyperthermophilic |
| 67770 | positive | growth | 90 | hyperthermophilic |
Isolation, sampling and environmental information
isolation
| @ref | sample type | geographic location | country | origin.country | continent |
|---|---|---|---|---|---|
| 24518 | hot spring | Kamchatka | USSR | RUS | Asia |
| 67770 | Thermal spring of Kamchatka and Kunashir Island |
isolation source categories
| Cat1 | Cat2 | Cat3 |
|---|---|---|
| #Environmental | #Aquatic | #Thermal spring |
| #Condition | #Thermophilic (>45°C) |
Safety information
risk assessment
- @ref: 24518
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
| @ref | description | accession | length | database | NCBI tax ID |
|---|---|---|---|---|---|
| 24518 | Desulfurococcus amylolyticus 16S ribosomal RNA gene, partial sequence | AF250331 | 1405 | ena | 1150674 |
| 67770 | Desulfurococcus amylolyticus gene for 16S rRNA, partial sequence, strain: JCM 9188 | AB661712 | 1461 | ena | 1150674 |
Genome sequences
- @ref: 66792
- description: Desulfurococcus amylolyticus DSM 3822
- accession: 2516143104
- assembly level: draft
- database: img
- NCBI tax ID: 1150674
GC content
| @ref | GC-content | method |
|---|---|---|
| 24518 | 41.2 | |
| 24518 | 45.2 | sequence analysis |
| 67770 | 41 | thermal denaturation, midpoint method (Tm) |
| 67770 | 43.2 | high performance liquid chromatography (HPLC) |
External links
@ref: 24518
culture collection no.: DSM 3822, JCM 9188
straininfo link
- @ref: 91032
- straininfo: 47764
literature
- topic: Metabolism
- Pubmed-ID: 29685348
- title: GH57 amylopullulanase from Desulfurococcus amylolyticus JCM 9188 can make highly branched cyclodextrin via its transglycosylation activity.
- authors: Park YU, Jung JH, Seo DH, Jung DH, Kim JH, Seo EJ, Baek NI, Park CS
- journal: Enzyme Microb Technol
- DOI: 10.1016/j.enzmictec.2018.03.005
- year: 2018
- mesh: Archaeal Proteins/*chemistry/genetics/metabolism, Cloning, Molecular, Cyclodextrins/chemistry/*metabolism, Desulfurococcaceae/chemistry/*enzymology/genetics, Enzyme Stability, Glucans/metabolism, Glycoside Hydrolases/*chemistry/genetics/metabolism, Glycosylation, Maltose/analogs & derivatives/chemistry/metabolism, Molecular Weight, Open Reading Frames, Substrate Specificity, Trisaccharides/metabolism
- topic2: Enzymology
Reference
| @id | authors | title | doi/url | catalogue |
|---|---|---|---|---|
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | 10.1099/ijsem.0.004332 | |
| 24518 | Curators of the DSMZ | https://www.dsmz.de/collection/catalogue/details/culture/DSM-3822 | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 3822) | |
| 66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | https://diaspora-project.de/progress.html#genomes | |
| 67770 | Curators of the JCM | https://jcm.brc.riken.jp/en/ | ||
| 91032 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | StrainInfo: A central database for resolving microbial strain identifiers | 10.60712/SI-ID47764.1 |