Strain identifier

BacDive ID: 132682

Type strain: Yes

Species: Allofrancisella inopinata

Strain history: <- W.-J. Li, Sun Yat-Sen University, Guangzhou, China <- P.-H. Qu, Guangdong Provincial Hospital; SYSU YG23

NCBI tax ID(s): 1085647 (species)

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General

@ref: 24471

BacDive-ID: 132682

DSM-Number: 101834

keywords: genome sequence, 16S sequence, Bacteria, aerobe, Gram-negative, coccus-shaped

description: Allofrancisella inopinata DSM 101834 is an aerobe, Gram-negative, coccus-shaped bacterium that was isolated from water of a cooling tower.

NCBI tax id

  • NCBI tax id: 1085647
  • Matching level: species

strain history

  • @ref: 24471
  • history: <- W.-J. Li, Sun Yat-Sen University, Guangzhou, China <- P.-H. Qu, Guangdong Provincial Hospital; SYSU YG23

doi: 10.13145/bacdive132682.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Beggiatoales
  • family: Francisellaceae
  • genus: Allofrancisella
  • species: Allofrancisella inopinata
  • full scientific name: Allofrancisella inopinata Qu et al. 2016

@ref: 24471

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Thiotrichales

family: Francisellaceae

genus: Allofrancisella

species: Allofrancisella inopinata

full scientific name: Allofrancisella inopinata Qu et al. 2016

type strain: yes

Morphology

cell morphology

  • @ref: 25033
  • gram stain: negative
  • cell shape: coccus-shaped
  • motility: no

Culture and growth conditions

culture medium

  • @ref: 24471
  • name: COLUMBIA-GLUCOSE-CYSTEINE-AGAR (DSMZ Medium 429c)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/429c
  • composition: Name: COLUMBIA-GLUCOSE-CYSTEINE-AGAR (DSMZ Medium 429c) Composition: Horse blood 40.0 g/l Sheep blood 40.0 g/l Glucose 10.0 g/l Cysteine 1.0 g/l Columbia agar base

culture temp

@refgrowthtypetemperaturerange
25033positivegrowth18-37
25033positiveoptimum32mesophilic
24471positivegrowth30mesophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 25033
  • oxygen tolerance: aerobe

spore formation

  • @ref: 25033
  • spore formation: no

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
250334853esculin-hydrolysis
250335291gelatin-hydrolysis
2503317306maltose-fermentation
2503317632nitrate-reduction
2503316634raffinose-fermentation
2503317992sucrose-fermentation
2503327897tryptophan-energy source
2503315824D-fructose+fermentation
2503317634D-glucose+fermentation
2503316024D-mannose+fermentation
2503317561L-cysteine+growth
6837717634D-glucose+builds acid from
6837715824D-fructose+builds acid from
6837718257ornithine-degradation
6837716199urea-hydrolysis
6837727897tryptophan-energy source

antibiotic resistance

@refChEBImetaboliteis antibioticis sensitiveis resistant
2503317698chloramphenicolyesyes
2503323066cephalosporinyesyes
25033100241ciprofloxacinyesyes
2503350845doxycyclineyesyes
2503317833gentamicinyesyes
2503363598levofloxacinyesyes
2503327902tetracyclineyesyes

metabolite production

@refChebi-IDmetaboliteproduction
2503315688acetoinno
2503335581indoleno
6837735581indoleno

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testindole test
2503315688acetoin-
2503335581indole-
6837735581indole-

enzymes

@refvalueactivityec
250336-phospho-beta-galactosidase-3.2.1.85
25033acid phosphatase+3.1.3.2
25033alanine arylamidase-3.4.11.2
25033alkaline phosphatase+3.1.3.1
25033alpha-arabinosidase-3.2.1.55
25033alpha-chymotrypsin-3.4.21.1
25033alpha-fucosidase-3.2.1.51
25033alpha-galactosidase-3.2.1.22
25033alpha-glucosidase-3.2.1.20
25033alpha-mannosidase-3.2.1.24
25033arginine arylamidase+
25033arginine dihydrolase-3.5.3.6
25033beta-galactosidase-3.2.1.23
25033beta-glucosidase-3.2.1.21
25033beta-glucuronidase-3.2.1.31
25033catalase+1.11.1.6
25033cystine arylamidase-3.4.11.3
25033cytochrome oxidase-1.9.3.1
25033esterase (C 4)+
25033esterase lipase (C 8)+
25033glutamyl-glutamate arylamidase-
25033glycin arylamidase-
25033histidine arylamidase-
25033leucine arylamidase+3.4.11.1
25033leucyl glycin arylamidase-3.4.11.1
25033lipase (C 14)-
25033N-acetyl-beta-glucosaminidase-3.2.1.52
25033naphthol-AS-BI-phosphohydrolase+
25033ornithine decarboxylase+4.1.1.17
25033phenylalanine arylamidase+
25033phenylalanine arylamidase-
25033proline-arylamidase-3.4.11.5
25033pyroglutamic acid arylamidase-
25033serine arylamidase-
25033trypsin-3.4.21.4
25033tryptophan deaminase-4.1.99.1
25033tyrosine arylamidase-
25033urease-3.5.1.5
25033valine arylamidase-
68377tryptophan deaminase-4.1.99.1
68377gamma-glutamyltransferase-2.3.2.2
68377proline-arylamidase-3.4.11.5
68377beta-galactosidase-3.2.1.23
68377alkaline phosphatase-3.1.3.1
68377lipase+
68377urease-3.5.1.5
68377ornithine decarboxylase-4.1.1.17
68377beta-lactamase-3.5.2.6

API NH

@refPENGLUFRUMALSACODCURELIPPALbeta GALProAGGTIND
24471-++++--+-----
24471-++-+/---+-----

Isolation, sampling and environmental information

isolation

  • @ref: 24471
  • sample type: water of a cooling tower
  • geographic location: Guangzhou city
  • country: China
  • origin.country: CHN
  • continent: Asia

isolation source categories

Cat1Cat2Cat3
#Engineered#Industrial#Cooling tower
#Environmental#Aquatic

taxonmaps

  • @ref: 69479
  • File name: preview.99_4694.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_68;96_1442;97_1720;98_2103;99_4694&stattab=map
  • Last taxonomy: Allofrancisella
  • 16S sequence: JN620412
  • Sequence Identity:
  • Total samples: 241
  • soil counts: 10
  • aquatic counts: 220
  • animal counts: 11

Safety information

risk assessment

  • @ref: 24471
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 24471
  • description: Allofrancisella inopinata strain SYSU YG23 16S ribosomal RNA gene, partial sequence
  • accession: JN620412
  • length: 1452
  • database: ena
  • NCBI tax ID: 1085647

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Allofrancisella inopinata DSM 101834GCA_004365555scaffoldncbi1085647
66792Allofrancisella inopinata strain DSM 1018341085647.3wgspatric1085647
66792Allofrancisella inopinata strain SYSU YG231085647.4completepatric1085647
66792Allofrancisella inopinata DSM 1018342784746783draftimg1085647

GC content

  • @ref: 24471
  • GC-content: 32.6
  • method: high performance liquid chromatography (HPLC)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
motileno90.446yes
flagellatedno97.543no
gram-positiveno96.433yes
anaerobicno98.003yes
aerobicno63.458yes
halophileno66.329no
spore-formingno97.39no
thermophileno97.883yes
glucose-utilyes72.622no
glucose-fermentno51.16yes

External links

@ref: 24471

culture collection no.: DSM 101834, KCTC 42968, SYSU YG23

straininfo link

  • @ref: 90990
  • straininfo: 403532

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny27543089Allofrancisella inopinata gen. nov., sp. nov. and Allofrancisella frigidaquae sp. nov., isolated from water-cooling systems, and transfer of Francisella guangzhouensis Qu et al. 2013 to the new genus as Allofrancisella guangzhouensis comb. nov.Qu PH, Li Y, Salam N, Chen SY, Liu L, Gu Q, Fang BZ, Xiao M, Li M, Chen C, Li WJInt J Syst Evol Microbiol10.1099/ijsem.0.0014372016Bacterial Typing Techniques, Base Composition, China, DNA, Bacterial/genetics, Fatty Acids/chemistry, Francisella, Gammaproteobacteria/*classification/genetics/isolation & purification, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Ubiquinone/chemistry, *Water Microbiology, *Water SupplyTranscriptome
Genetics33239460Complete Genome Sequences of Allofrancisella inopinata SYSU YG23 and Allofrancisella frigidaquae SYSU 10HL1970, Isolated from Water from Cooling Systems in China.Ohrman C, Uneklint I, Karlsson L, Svensson D, Forsman M, Sjodin AMicrobiol Resour Announc10.1128/MRA.00554-202020Phylogeny

Reference

@idauthorstitledoi/urlcataloguejournalpubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
24471Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-101834Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 101834)
25033P. H. L. Qu, Y.,Salam, N.,Chen, S. Y.,Liu, L.,Gu, Q.,Fang, B. Z.,Xiao, M.,Li, M.,Chen, C.,Li, W. J.Allofrancisella inopinata gen. nov., sp. nov. and Allofrancisella frigidaquae sp. nov., isolated from water-cooling systems, and transfer of Francisella guangzhouensis Qu et al. 2013 to the new genus as Allofrancisella guangzhouensis comb. nov10.1099/ijsem.0.001437IJSEM 66: 4832-4838 201627543089
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
68377Automatically annotated from API NH
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.MicrobeAtlas 1.0 betahttps://microbeatlas.org/
90990Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID403532.1