Strain identifier

BacDive ID: 132621

Type strain: Yes

Species: Brevitalea deliciosa

Strain Designation: Ac_16_C4

Strain history: <- A. Geppert, Leibniz-Institut DSMZ GmbH, Braunschweig, Germany; Ac_16_C4 <- A. Geppert and P. Kaul

NCBI tax ID(s): 1603870 (species)

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General

@ref: 24410

BacDive-ID: 132621

DSM-Number: 29892

keywords: 16S sequence, Bacteria, obligate aerobe, chemoorganoheterotroph, Gram-negative, rod-shaped, colony-forming

description: Brevitalea deliciosa Ac_16_C4 is an obligate aerobe, chemoorganoheterotroph, Gram-negative bacterium that forms circular colonies and was isolated from non-irrigated semi-arid agricultural sandy soil.

NCBI tax id

  • NCBI tax id: 1603870
  • Matching level: species

strain history

  • @ref: 24410
  • history: <- A. Geppert, Leibniz-Institut DSMZ GmbH, Braunschweig, Germany; Ac_16_C4 <- A. Geppert and P. Kaul

doi: 10.13145/bacdive132621.20240510.9

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/acidobacteriota
  • domain: Bacteria
  • phylum: Acidobacteriota
  • class: Blastocatellia
  • order: Blastocatellales
  • family: Arenimicrobiaceae
  • genus: Brevitalea
  • species: Brevitalea deliciosa
  • full scientific name: Brevitalea deliciosa Wüst et al. 2016

@ref: 24410

domain: Bacteria

phylum: Acidobacteria

class: Blastocatellia

order: Blastocatellales

family: Arenimicrobiaceae

genus: Brevitalea

species: Brevitalea deliciosa

full scientific name: Brevitalea deliciosa Wüst et al. 2016

strain designation: Ac_16_C4

type strain: yes

Morphology

cell morphology

  • @ref: 43144
  • gram stain: negative
  • cell length: 0.9-1.8 µm
  • cell width: 0.6-0.8 µm
  • cell shape: rod-shaped
  • motility: no

colony morphology

  • @ref: 43144
  • colony size: 1 mm
  • colony color: white
  • colony shape: circular
  • medium used: Solid media

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
43144Solid mediayes
24410SSE/HD1:10 (DSMZ Medium 1426)yeshttps://mediadive.dsmz.de/medium/1426Name: SSE/HD 1:10 MEDIUM (DSMZ Medium 1426) Composition: MES 1.95 g/l Peptone 0.5 g/l CaSO4 x 2 H2O 0.4303 g/l MgSO4 x 7 H2O 0.3695 g/l Yeast extract 0.25 g/l Na NO3 0.212 g/l CaCl2 x 2 H2O 0.1469 g/l Ca(NO3)2 x 4 H2O 0.118 g/l MgCl2 x 6 H2O 0.1018 g/l Glucose 0.1 g/l (NH4)2SO4 0.09915 g/l NH4Cl 0.05345 g/l K2SO4 0.0435 g/l FeSO4 x 7 H2O 0.00555 g/l KH2PO4 0.00340213 g/l HCl 0.0025 g/l FeCl2 x 4 H2O 0.0015 g/l CoCl2 x 6 H2O 0.00019 g/l MnCl2 x 4 H2O 0.0001 g/l ZnCl2 7e-05 g/l Na2MoO4 x 2 H2O 3.6e-05 g/l NiCl2 x 6 H2O 2.4e-05 g/l Pyridoxine hydrochloride 1e-05 g/l H3BO3 6e-06 g/l Lipoic acid 5e-06 g/l D-Calcium pantothenate 5e-06 g/l Nicotinic acid 5e-06 g/l Riboflavin 5e-06 g/l Thiamine-HCl x 2 H2O 5e-06 g/l p-Aminobenzoic acid 5e-06 g/l Folic acid 2e-06 g/l Biotin 2e-06 g/l CuCl2 x 2 H2O 2e-06 g/l Vitamin B12 1e-07 g/l Distilled water

culture temp

@refgrowthtypetemperature
43144positiveoptimum32.9-37.2
43144positivegrowth24.6-34.2
24410positivegrowth28

culture pH

@refabilitytypepHPH range
43144positivegrowth4.7-9.0alkaliphile
43144positiveoptimum5.4-6.5

Physiology and metabolism

oxygen tolerance

  • @ref: 43144
  • oxygen tolerance: obligate aerobe

nutrition type

  • @ref: 43144
  • type: chemoorganoheterotroph

spore formation

  • @ref: 43144
  • type of spore: spore
  • spore formation: no

halophily

@refsaltgrowthtested relationconcentration
43144NaClpositivemaximum0.5 %(w/v)
43144NaClpositiveoptimum0.25 %

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
43144casamino acids+growth
43144casein hydrolysate+growth
43144peptone+growth
43144yeast extract+growth
43144cellulose-growth62968
43144xylan-growth37166
43144lyxose-growth25097
43144maltose-growth17306
43144sucrose-growth17992
43144mannitol-growth29864
43144succinamate-growth143136
43144arabinose-growth22599
43144cellobiose-growth17057
43144erythritol-growth17113
43144erythrulose-growth23958
43144fructose-growth28757
43144fucose-growth33984
43144galactose-growth28260
43144glucose-growth17234
43144lactose-growth17716
43144mannose-growth37684
43144melezitose-growth6731
43144raffinose-growth16634
43144rhamnose-growth26546
43144sorbose-growth27922
43144trehalose-growth27082
43144xylose-growth18222
43144glucosamine-growth5417
43144N-acetylglucosamine-growth506227
43144N-acetylgalactosamine-growth28037
43144acetoin-growth15688
43144galactitol-growth16813
43144arabinitol-growth22605
43144myo-inositol-growth17268
43144ribitol-growth15963
43144sorbitol-growth30911
43144xylitol-growth17151
43144alanine-growth16449
43144arginine-growth29016
43144asparagine-growth22653
43144cysteine-growth15356
43144glutamine-growth28300
43144glycine-growth15428
43144histidine-growth27570
43144isoleucine-growth24898
43144leucine-growth25017
43144lysine-growth25094
43144methionine-growth16811
43144ornithine-growth18257
43144phenylalanine-growth28044
43144proline-growth26271
43144serine-growth17822
43144threonine-growth26986
43144tryptophan-growth27897
43144tyrosine+/-growth18186
43144valine-growth27266
43144adipate-growth17128
43144acetate-growth30089
43144ascorbate-growth22651
43144aspartate-growth35391
43144benzoate-growth16150
43144trimethoxybenzoate-growth
43144butyrate-growth17968
431442-hydroxybutyrate-growth64552
43144beta-hydroxybutyrate-growth8295
431444-hydroxybutyrate-growth16724
43144isobutyrate-growth48944
43144decanoate-growth27689
43144octanoate-growth25646
43144citrate-growth16947
43144isocitrate-growth16087
43144crotonate-growth35899
43144formate-growth15740
43144fumarate-growth29806
43144gluconate-growth24265
431442-oxogluconate-growth
43144glucuronate-growth24297
431442-oxoglutarate-growth30916
43144glutamate-growth29987
43144glycolate-growth29805
43144glyoxylate-growth36655
43144heptanoate-growth32362
43144isovalerate-growth48942
431444-oxopentanoate-growth39150
43144lactate-growth24996
43144malate-growth25115
43144maleic acid-growth18300
43144malonate-growth15792
43144nicotinate-growth32544
43144oxaloacetate-growth16452
43144propionate-growth17272
43144protocatechuate+/-growth36241
43144pyruvate-growth15361
43144shikimate-growth36208
43144tartrate-growth30929
431442-oxopentanoate-growth28644
43144butanol-growth28885
431441,2-butandiol-growth52682
431442,3-butanediol-growth62064
43144ethanol-growth16236
43144ethylene-growth29362
43144glycol-growth13643
43144glycerol-growth17754
43144methanol-growth17790
43144propanol-growth28831
431441,2-propandiol-growth16997
43144carboxymethylcellulose-growth85146
43144chitin-growth17029
43144fermented rumen extract-growth
43144laminarin-growth6364
43144lignin-growth6457
43144pectin-growth17309
43144tween 80-growth53426
43144starch+growth28017
43144esculin+hydrolysis4853
43144nitrate-reduction17632
43144glucose-fermentation17234
431444-nitrophenyl beta-D-galactopyranoside hydrolysate-hydrolysis
43144urea-hydrolysis16199
43144gelatin-hydrolysis5291
43144cellulose-hydrolysis62968
43144starch+hydrolysis28017
43144xylan-hydrolysis37166
68369malate-assimilation25115
68369adipate-assimilation17128
68369decanoate-assimilation27689
68369gluconate-assimilation24265
68369maltose-assimilation17306
68369N-acetylglucosamine-assimilation59640
68369D-mannitol-assimilation16899
68369D-mannose-assimilation16024
68369L-arabinose-assimilation30849
68369D-glucose-assimilation17634
68369gelatin-hydrolysis5291
68369esculin+hydrolysis4853
68369urea-hydrolysis16199
68369arginine-hydrolysis29016
68369D-glucose-fermentation17634
68369tryptophan-energy source27897
68369nitrate-reduction17632

metabolite production

@refChebi-IDmetaboliteproduction
4314435581indoleno
6836935581indoleno

metabolite tests

  • @ref: 68369
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: -

enzymes

@refvalueactivityec
43144catalase-1.11.1.6
43144cytochrome oxidase-1.9.3.1
43144acid phosphatase+3.1.3.2
43144alkaline phosphatase+3.1.3.1
43144naphthol-AS-BI-phosphohydrolase+
43144esterase Lipase (C 8)+
43144leucine arylamidase+3.4.11.1
43144valine arylamidase+
43144cystine arylamidase+/-3.4.11.3
43144trypsin+3.4.21.4
43144alpha-glucosidase+3.2.1.20
43144beta-glucosidase+3.2.1.21
43144N-acetyl-beta-glucosaminidase+3.2.1.52
43144esterase (C 4)+
43144alpha-galactosidase+/-3.2.1.22
68369cytochrome oxidase+1.9.3.1
68369gelatinase-
68369beta-glucosidase+3.2.1.21
68369urease-3.5.1.5
68369arginine dihydrolase-3.5.3.6

fatty acid profile

  • fatty acids

    @reffatty acidpercentage
    43144C15:0 iso49
    43144C17:0 iso4.6
    43144C17:0 anteiso8.5
    43144C15:1 iso h / C13:0 3OH16.7
  • type of FA analysis: whole cell analysis
  • incubation medium: SSE/HD 1:10
  • agar/liquid: liquid
  • incubation temperature: 28
  • incubation time: 7
  • incubation pH: 5.00
  • software version: Sherlock 6.1
  • system: MIS MIDI

API 20NE

@refNO3TRPGLU_ FermADH ArgUREESCGELPNPGGLU_ AssimARAMNEMANNAGMALGNTCAPADIMLTCITPACOX
24410-----+-+------------+

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentlatitudelongitude
24410non-irrigated semi-arid agricultural sandy soilKavango region, Mashare (17° 54' 9.2'' S, 20° 14' 4.5'' E)NamibiaNAMAfrica-17.902620.2346
43144sandy agriculturally used Kalahari soilMashare, NamibiaNamibiaNAMAfrica-1720

isolation source categories

Cat1Cat2Cat3
#Engineered#Agriculture
#Environmental#Terrestrial#Sandy
#Environmental#Terrestrial#Soil
#Climate#Hot#Semiarid

taxonmaps

  • @ref: 69479
  • File name: preview.99_86082.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_772;96_35838;97_45523;98_59586;99_86082&stattab=map
  • Last taxonomy: Brevitalea deliciosa
  • 16S sequence: KP638490
  • Sequence Identity:
  • Total samples: 395
  • soil counts: 348
  • aquatic counts: 5
  • animal counts: 9
  • plant counts: 33

Safety information

risk assessment

  • @ref: 24410
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 24410
  • description: Brevitalea deliciosa strain Ac_16_C4 16S ribosomal RNA gene, partial sequence
  • accession: KP638490
  • length: 1528
  • database: nuccore
  • NCBI tax ID: 1603870

GC content

  • @ref: 24410
  • GC-content: 54.7
  • method: high performance liquid chromatography (HPLC)

External links

@ref: 24410

culture collection no.: DSM 29892, LMG 28995, LMG 29713

straininfo link

  • @ref: 90935
  • straininfo: 396681

literature

  • topic: Phylogeny
  • Pubmed-ID: 27255677
  • title: Brevitalea aridisoli, B. deliciosa and Arenimicrobium luteum, three novel species of Acidobacteria subdivision 4 (class Blastocatellia) isolated from savanna soil and description of the novel family Pyrinomonadaceae.
  • authors: Wust PK, Foesel BU, Geppert A, Huber KJ, Luckner M, Wanner G, Overmann J
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijsem.0.001199
  • year: 2016
  • mesh: Acidobacteria/*classification/genetics/isolation & purification, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, Grassland, Namibia, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Soil Microbiology, Vitamin K 2/analogs & derivatives/chemistry
  • topic2: Transcriptome

Reference

@idauthorstitledoi/urlcataloguejournalpubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
24410Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-29892Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 29892)
43144Pia K. Wüst, Bärbel U. Foesel, Alicia Geppert, Katharina J. Huber, Manja Luckner, Gerhard Wanner and Jörg OvermannBrevitalea aridisoli, B. deliciosa and Arenimicrobium luteum, three novel species of Acidobacteria subdivision 4 (class Blastocatellia) isolated from savanna soil and description of the novel family Pyrinomonadaceae10.1099/ijsem.0.001199IJSEM 66: 3355-3366 201627255677
68369Automatically annotated from API 20NE
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.MicrobeAtlas 1.0 betahttps://microbeatlas.org/
90935Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID396681.1