Strain identifier

BacDive ID: 132590

Type strain: Yes

Species: Tepidibacillus infernus

Strain Designation: MBL-TLP

Strain history: <- O. A. Podosokorskaya, Winogradsky Inst. Microbiol., RAS, Moscow, Russia; MBI-TLP <- O. A. Podosokorskaya {2013}

NCBI tax ID(s): 1806172 (species)

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General

@ref: 24379

BacDive-ID: 132590

DSM-Number: 28123

keywords: 16S sequence, Bacteria, aerotolerant, organotroph, spore-forming, thermophilic, Gram-positive, motile, rod-shaped

description: Tepidibacillus infernus MBL-TLP is an aerotolerant, organotroph, spore-forming bacterium that was isolated from microbial mat under the flow of subsurface water in a gold mine.

NCBI tax id

  • NCBI tax id: 1806172
  • Matching level: species

strain history

  • @ref: 24379
  • history: <- O. A. Podosokorskaya, Winogradsky Inst. Microbiol., RAS, Moscow, Russia; MBI-TLP <- O. A. Podosokorskaya {2013}

doi: 10.13145/bacdive132590.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Caryophanales
  • family: Bacillaceae
  • genus: Tepidibacillus
  • species: Tepidibacillus infernus
  • full scientific name: Tepidibacillus infernus Podosokorskaya et al. 2016

@ref: 24379

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Bacillales

family: Bacillaceae

genus: Tepidibacillus

species: Tepidibacillus infernus

full scientific name: Tepidibacillus infernus Podosokorskaya et al. 2016

strain designation: MBL-TLP

type strain: yes

Morphology

cell morphology

  • @ref: 43090
  • gram stain: positive
  • cell length: 3-15 µm
  • cell width: 0.25-0.5 µm
  • cell shape: rod-shaped
  • motility: yes
  • flagellum arrangement: polar

Culture and growth conditions

culture medium

  • @ref: 24379
  • name: TEPIDIBACILLUS MEDIUM (DSMZ Medium 1636)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/1636
  • composition: Name: TEPIDIBACILLUS MEDIUM (DSMZ Medium 1636) Composition: D-Glucose 1.99601 g/l Na2CO3 1.49701 g/l CaCl2 x 2 H2O 0.329341 g/l KH2PO4 0.329341 g/l MgCl2 x 6 H2O 0.329341 g/l KCl 0.329341 g/l NH4Cl 0.329341 g/l Na2S x 9 H2O 0.299401 g/l Yeast extract 0.0998004 g/l MgSO4 x 7 H2O 0.00299401 g/l Nitrilotriacetic acid 0.00149701 g/l NaCl 0.000998004 g/l MnSO4 x H2O 0.000499002 g/l CoSO4 x 7 H2O 0.000179641 g/l ZnSO4 x 7 H2O 0.000179641 g/l Pyridoxine hydrochloride 9.98004e-05 g/l FeSO4 x 7 H2O 9.98004e-05 g/l (DL)-alpha-Lipoic acid 4.99002e-05 g/l Calcium D-(+)-pantothenate 4.99002e-05 g/l p-Aminobenzoic acid 4.99002e-05 g/l Nicotinic acid 4.99002e-05 g/l Riboflavin 4.99002e-05 g/l Thiamine HCl 4.99002e-05 g/l NiCl2 x 6 H2O 2.99401e-05 g/l AlK(SO4)2 x 12 H2O 1.99601e-05 g/l Folic acid 1.99601e-05 g/l Biotin 1.99601e-05 g/l Na2MoO4 x 2 H2O 9.98004e-06 g/l CuSO4 x 5 H2O 9.98004e-06 g/l H3BO3 9.98004e-06 g/l Vitamin B12 9.98004e-07 g/l Na2WO4 x 2 H2O 3.99202e-07 g/l Na2SeO3 x 5 H2O 2.99401e-07 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
43090positiveoptimum50thermophilic
43090positivegrowth25-58
24379positivegrowth50thermophilic

culture pH

@refabilitytypepHPH range
43090positivegrowth5.6-8.8alkaliphile
43090positiveoptimum7.4

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
43090aerotolerant
43090anaerobe

nutrition type

  • @ref: 43090
  • type: organotroph

spore formation

  • @ref: 43090
  • type of spore: spore
  • spore formation: yes

halophily

@refsaltgrowthtested relationconcentration
43090NaClpositivegrowth0-8.5 %(w/v)
43090NaClpositiveoptimum1.5 %(w/v)

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
4309017234glucose+fermentation
4309017306maltose+fermentation
4309017057cellobiose+fermentation
4309017992sucrose+fermentation
4309027082trehalose+fermentation
4309028757fructose+fermentation
4309018222xylose+fermentation
4309023652dextrin+fermentation
4309028017starch+fermentation
4309010057xanthan+fermentation
43090yeast extract+fermentation
4309030089acetate+oxidation
4309028017starch+oxidation
43090yeast extract+oxidation
4309016094thiosulfate+reduction
4309033403elemental sulfur+reduction
4309017632nitrate+reduction
4309029125arsenate+reduction
4309015075selenate+reduction
4309018212selenite+reduction
4309016189sulfate-reduction
4309017359sulfite-reduction
4309016301nitrite-reduction
43090ferrihydrite-reduction
4309028260galactose-growth
4309022599arabinose-growth
4309017716lactose-growth
4309062968cellulose-growth
4309052071dextran-growth
4309017309pectin-growth
4309017029chitin-growth
4309037166xylan-growth
4309058187alginate-growth
430902511agarose-growth
43090peptone-growth
43090tryptone-growth
430905291gelatin-growth
43090casein-growth
4309017790methanol-growth
4309016236ethanol-growth
4309015740formate-growth
4309015361pyruvate-growth
4309029806fumarate-growth
4309024996lactate-growth
4309017754glycerol-growth
4309017272propionate-growth
430906452lichenin-growth

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentlatitudelongitudesampling date
24379microbial mat under the flow of subsurface water in a gold mineTauTona gold mineSouth AfricaZAFAfrica-26.416127.4275
43090microbial mat of TauTona gold mineWitwatersrand Basin, near Carletonville, South Africa-26272013-04-17

isolation source categories

Cat1Cat2Cat3
#Engineered#Other#Mine
#Environmental#Aquatic
#Environmental#Microbial community#Microbial mat

taxonmaps

  • @ref: 69479
  • File name: preview.99_32359.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_3297;96_15464;97_19205;98_23972;99_32359&stattab=map
  • Last taxonomy: Tepidibacillus
  • 16S sequence: KU726543
  • Sequence Identity:
  • Total samples: 170
  • soil counts: 28
  • aquatic counts: 136
  • animal counts: 6

Safety information

risk assessment

  • @ref: 24379
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 24379
  • description: Tepidibacillus infernus strain MBL-TLP 16S ribosomal RNA gene, partial sequence
  • accession: KU726543
  • length: 1412
  • database: ena
  • NCBI tax ID: 1806172

GC content

@refGC-contentmethod
4309035.4genome sequence analysis
2437935.4sequence analysis

External links

@ref: 24379

culture collection no.: DSM 28123, VKM B-2949

straininfo link

  • @ref: 90907
  • straininfo: 397813

literature

  • topic: Phylogeny
  • Pubmed-ID: 27216447
  • title: Tepidibacillus infernus sp. nov., a moderately thermophilic, selenate- and arsenate-respiring hydrolytic bacterium isolated from a gold mine, and emended description of the genus Tepidibacillus.
  • authors: Podosokorskaya OA, Merkel AY, Gavrilov SN, Fedoseev I, Heerden EV, Cason ED, Novikov AA, Kolganova TV, Korzhenkov AA, Bonch-Osmolovskaya EA, Kublanov IV
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijsem.0.001166
  • year: 2016
  • mesh: Arsenates/metabolism, Bacillaceae/*classification/genetics/isolation & purification, Bacteria, Anaerobic/classification/genetics/isolation & purification, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, Gold, *Mining, *Phylogeny, RNA, Ribosomal, 16S/genetics, Selenic Acid/metabolism, Sequence Analysis, DNA, South Africa
  • topic2: Metabolism

Reference

@idauthorstitledoi/urlcataloguejournalpubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
24379Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-28123Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 28123)
43090Olga A. Podosokorskaya, Alexander Y. Merkel, Sergey N. Gavrilov, Igor Fedoseev, Esta van Heerden, Errol D. Cason, Andrey A. Novikov, Tatyana V. Kolganova, Aleksei A. Korzhenkov, Elizaveta A. Bonch-Osmolovskaya and Ilya V. KublanovTepidibacillus infernus sp. nov., a moderately thermophilic, selenate- and arsenate-respiring hydrolytic bacterium isolated from a gold mine, and emended description of the genus Tepidibacillus10.1099/ijsem.0.001166IJSEM 66: 3189-3194 201627216447
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.MicrobeAtlas 1.0 betahttps://microbeatlas.org/
90907Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID397813.1