Tepidibacillus infernus (DSM 28123, VKM B-2949, MBL-TLP)

Name: Tepidibacillus infernus (DSM 28123, VKM B-2949, MBL-TLP)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 132590

Type strain

Strain Designation MBL-TLP

Culture col. no. DSM 28123 VKM B-2949 MBL-TLP

NCBI tax ID(s) 1806172

Special Collections DSMZ Literature strains

History <- O. A. Podosokorskaya, Winogradsky Inst. Microbiol., RAS, Moscow, Russia; MBI-TLP <- O. A. Podosokorskaya {2013}

Links

Tepidibacillus infernus MBL-TLP is an aerotolerant, organotroph, spore-forming bacterium that was isolated from microbial mat under the flow of subsurface water in a gold mine.

spore-forming Gram-positive motile rod-shaped aerotolerant organotroph 16S sequence Bacteria

Name and taxonomic classification

SI-ID 397813

VKM B-2949,DSM 28123

@ref 20215

Last LPSN update 2026-02-09 11:31:45

Domain Bacteria

Phylum Bacillota

Class Bacilli

Order Caryophanales

Family Bacillaceae

Genus Tepidibacillus

Species Tepidibacillus infernus

Full scientific name Tepidibacillus infernus Podosokorskaya et al. 2016

Morphology

Cell morphology

@ref	Gram stain	Cell length	Cell width	Cell shape	Motility	Flagellum arrangement
43090	positive	3-15 µm	0.25-0.5 µm	rod-shaped		polar

Culture and growth conditions

Culture medium

@ref	Name	Growth	Medium link	Composition
24379	TEPIDIBACILLUS MEDIUM (DSMZ Medium 1636)		Medium recipe at MediaDive	Name: TEPIDIBACILLUS MEDIUM (DSMZ Medium 1636) Composition: D-Glucose 1.99601 g/l Na2CO3 1.49701 g/l CaCl2 x 2 H2O 0.329341 g/l KH2PO4 0.329341 g/l MgCl2 x 6 H2O 0.329341 g/l KCl 0.329341 g/l NH4Cl 0.329341 g/l Na2S x 9 H2O 0.299401 g/l Yeast extract 0.0998004 g/l MgSO4 x 7 H2O 0.00299401 g/l Nitrilotriacetic acid 0.00149701 g/l NaCl 0.000998004 g/l MnSO4 x H2O 0.000499002 g/l CoSO4 x 7 H2O 0.000179641 g/l ZnSO4 x 7 H2O 0.000179641 g/l Pyridoxine hydrochloride 9.98004e-05 g/l FeSO4 x 7 H2O 9.98004e-05 g/l (DL)-alpha-Lipoic acid 4.99002e-05 g/l Calcium D-(+)-pantothenate 4.99002e-05 g/l p-Aminobenzoic acid 4.99002e-05 g/l Nicotinic acid 4.99002e-05 g/l Riboflavin 4.99002e-05 g/l Thiamine HCl 4.99002e-05 g/l NiCl2 x 6 H2O 2.99401e-05 g/l AlK(SO4)2 x 12 H2O 1.99601e-05 g/l Folic acid 1.99601e-05 g/l Biotin 1.99601e-05 g/l Na2MoO4 x 2 H2O 9.98004e-06 g/l CuSO4 x 5 H2O 9.98004e-06 g/l H3BO3 9.98004e-06 g/l Vitamin B12 9.98004e-07 g/l Na2WO4 x 2 H2O 3.99202e-07 g/l Na2SeO3 x 5 H2O 2.99401e-07 g/l Distilled water

Culture temp

@ref	Growth	Type	Temperature (°C)
24379	positive	growth	50
43090	positive	optimum	50
43090	positive	growth	25-58

Culture pH

@ref	Ability	Type	PH	PH range
43090	positive	optimum	7.4
43090	positive	growth	5.6-8.8	alkaliphile

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance
43090	aerotolerant
43090	anaerobe

Nutrition type

43090

Typeorganotroph

Spore formation

@ref	Type of spore	Spore formation
43090	spore

Halophily

@ref	Salt	Growth	Tested relation	Concentration
43090	NaCl	positive	growth	0-8.5 %(w/v)
43090	NaCl	positive	optimum	1.5 %(w/v)

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
43090	30089 ChEBI	acetate	+	oxidation
43090	2511 ChEBI	agarose	-	growth
43090	58187 ChEBI	alginate	-	growth
43090	22599 ChEBI	arabinose	-	growth
43090	29125 ChEBI	arsenate	+	reduction
43090		casein	-	growth
43090	17057 ChEBI	cellobiose	+	fermentation
43090	62968 ChEBI	cellulose	-	growth
43090	17029 ChEBI	chitin	-	growth
43090	52071 ChEBI	dextran	-	growth
43090	23652 ChEBI	dextrin	+	fermentation
43090	33403 ChEBI	elemental sulfur	+	reduction
43090	16236 ChEBI	ethanol	-	growth
43090		ferrihydrite	-	reduction
43090	15740 ChEBI	formate	-	growth
43090	28757 ChEBI	fructose	+	fermentation
43090	29806 ChEBI	fumarate	-	growth
43090	28260 ChEBI	galactose	-	growth
43090	5291 ChEBI	gelatin	-	growth
43090	17234 ChEBI	glucose	+	fermentation
43090	17754 ChEBI	glycerol	-	growth
43090	24996 ChEBI	lactate	-	growth
43090	17716 ChEBI	lactose	-	growth
43090	6452 ChEBI	lichenin	-	growth
43090	17306 ChEBI	maltose	+	fermentation
43090	17790 ChEBI	methanol	-	growth
43090	17632 ChEBI	nitrate	+	reduction
43090	16301 ChEBI	nitrite	-	reduction
43090	17309 ChEBI	pectin	-	growth
43090		peptone	-	growth
43090	17272 ChEBI	propionate	-	growth
43090	15361 ChEBI	pyruvate	-	growth
43090	15075 ChEBI	selenate	+	reduction
43090	18212 ChEBI	selenite	+	reduction
43090	28017 ChEBI	starch	+	fermentation
43090	28017 ChEBI	starch	+	oxidation
43090	17992 ChEBI	sucrose	+	fermentation
43090	16189 ChEBI	sulfate	-	reduction
43090	17359 ChEBI	sulfite	-	reduction
43090	16094 ChEBI	thiosulfate	+	reduction
43090	27082 ChEBI	trehalose	+	fermentation
43090		tryptone	-	growth
43090	10057 ChEBI	xanthan	+	fermentation
43090	37166 ChEBI	xylan	-	growth
43090	18222 ChEBI	xylose	+	fermentation
43090		yeast extract	+	fermentation
43090		yeast extract	+	oxidation

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Engineered	#Other	#Mine
#Environmental	#Aquatic	-
#Environmental	#Microbial community	#Microbial mat

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent	Latitude	Longitude	Sampling date
24379	microbial mat under the flow of subsurface water in a gold mine	TauTona gold mine	South Africa	ZAF	Africa	-26.4161	27.4275 -26.4161/27.4275
43090	microbial mat of TauTona gold mine	Witwatersrand Basin, near Carletonville, South Africa				-26	27 -26/27	2013-04-17

Taxonmaps

69479

Global distribution of 16S sequence KU726543 (>99% sequence identity) for Tepidibacillus from Microbeatlas

Aquatic Samples	136
Soil Samples	28
Animal Samples	6
Plant Samples
Total Samples	170

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
24379	1	Risk group (German classification)

Sequence information

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
24379	Tepidibacillus infernus strain MBL-TLP 16S ribosomal RNA gene, partial sequence	KU726543	1412		1806172

GC content

@ref	GC-content (mol%)	Method
43090	35.4	genome sequence analysis
24379	35.4	sequence analysis

Genome-based predictions

Literature

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Tepidibacillus infernus sp. nov., a moderately thermophilic, selenate- and arsenate-respiring hydrolytic bacterium isolated from a gold mine, and emended description of the genus Tepidibacillus.	Podosokorskaya OA, Merkel AY, Gavrilov SN, Fedoseev I, Heerden EV, Cason ED, Novikov AA, Kolganova TV, Korzhenkov AA, Bonch-Osmolovskaya EA, Kublanov IV	Int J Syst Evol Microbiol	10.1099/ijsem.0.001166	2016

References

#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#24379	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 28123
#43090	Olga A. Podosokorskaya, Alexander Y. Merkel, Sergey N. Gavrilov, Igor Fedoseev, Esta van Heerden, Errol D. Cason, Andrey A. Novikov, Tatyana V. Kolganova, Aleksei A. Korzhenkov, Elizaveta A. Bonch-Osmolovskaya and Ilya V. Kublanov: Tepidibacillus infernus sp. nov., a moderately thermophilic, selenate- and arsenate-respiring hydrolytic bacterium isolated from a gold mine, and emended description of the genus Tepidibacillus. IJSEM 66: 3189 - 3194 2016 ( DOI 10.1099/ijsem.0.001166 , PubMed 27216447 )
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Tepidibacillus infernus (DSM 28123, VKM B-2949, MBL-TLP)

Name and taxonomic classification

Morphology

Cell morphology

Culture and growth conditions

Culture medium

Culture temp

Culture pH

Physiology and metabolism

Oxygen tolerance

Nutrition type

Spore formation

Halophily

Metabolite utilization

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

16S sequences

GC content

Genome-based predictions

Literature

Literature

References

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