Strain identifier

BacDive ID: 132550

Type strain: Yes

Species: Chania multitudinisentens

Strain Designation: RB-25

Strain history: <- R. Ee, Univ. of Malaya, Microbiome Lab., Kuala Lumpur, Malaysia; RB-25 <- R. Ee and Lim Yan Lue

NCBI tax ID(s): 1639108 (species)

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General

@ref: 24339

BacDive-ID: 132550

DSM-Number: 28811

keywords: genome sequence, 16S sequence, Bacteria, aerobe, mesophilic, Gram-negative, motile, rod-shaped

description: Chania multitudinisentens RB-25 is an aerobe, mesophilic, Gram-negative bacterium that was isolated from soil from an ex-landfill site.

NCBI tax id

  • NCBI tax id: 1639108
  • Matching level: species

strain history

  • @ref: 24339
  • history: <- R. Ee, Univ. of Malaya, Microbiome Lab., Kuala Lumpur, Malaysia; RB-25 <- R. Ee and Lim Yan Lue

doi: 10.13145/bacdive132550.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Enterobacterales
  • family: Yersiniaceae
  • genus: Chania
  • species: Chania multitudinisentens
  • full scientific name: Chania multitudinisentens Ee et al. 2016

  • synonyms

    • @ref: 20215
    • synonym: Serratia multitudinisentens

@ref: 24339

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Enterobacterales

family: Yersiniaceae

genus: Chania

species: Chania multitudinisentens

full scientific name: Chania multitudinisentens Ee et al. 2016

strain designation: RB-25

type strain: yes

Morphology

cell morphology

  • @ref: 43634
  • gram stain: negative
  • cell length: 7.5-9 µm
  • cell width: 3-4 µm
  • cell shape: rod-shaped
  • motility: yes

colony morphology

  • @ref: 43634
  • colony color: Pale white
  • incubation period: 1 day
  • medium used: Luria Bertani agar

multimedia

  • @ref: 24339
  • multimedia content: https://www.dsmz.de/microorganisms/photos/DSM_28811.jpg
  • intellectual property rights: © Leibniz-Institut DSMZ

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
43634LB (Luria-Bertani) MEDIUMyes
24339NUTRIENT AGAR (DSMZ Medium 1)yeshttps://mediadive.dsmz.de/medium/1Name: NUTRIENT AGAR (DSMZ Medium 1) Composition: Agar 15.0 g/l Peptone 5.0 g/l Meat extract 3.0 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
43634positiveoptimum28mesophilic
24339positivegrowth28mesophilic

culture pH

  • @ref: 43634
  • ability: positive
  • type: optimum
  • pH: 5-8

Physiology and metabolism

oxygen tolerance

  • @ref: 43634
  • oxygen tolerance: aerobe

compound production

  • @ref: 24339
  • compound: N acyl homoserine lactones

halophily

  • @ref: 43634
  • salt: NaCl
  • growth: positive
  • tested relation: optimum
  • concentration: 1-8 %(w/v)

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
4363417057cellobiose-builds acid from
4363428053melibiose-builds acid from
4363416651(S)-lactate+assimilation
4363415589L-malate+assimilation
43634172562-deoxyadenosine+assimilation
43634309162-oxoglutarate+assimilation
43634370543-hydroxybutyrate+assimilation
43634739183-O-methyl-D-glucose+assimilation
43634182404-hydroxy-L-proline+assimilation
43634181014-hydroxyphenylacetic acid+assimilation
4363430089acetate+assimilation
4363413705acetoacetate+assimilation
4363416335adenosine+assimilation
4363415963ribitol+assimilation
4363417925alpha-D-glucose+assimilation
4363422599arabinose+assimilation
4363418305arbutin+assimilation
4363440656beta-D-allose+assimilation
4363473706bromosuccinate+assimilation
4363417108D-arabinose+assimilation
4363418333D-arabitol+assimilation
4363415824D-fructose+assimilation
4363478697D-fructose 6-phosphate+assimilation
4363415895D-galactonic acid lactone+assimilation
4363412936D-galactose+assimilation
4363418024D-galacturonic acid+assimilation
436348391D-gluconate+assimilation
4363417315D-glucosamine+assimilation
4363414314D-glucose 6-phosphate+assimilation
4363415748D-glucuronate+assimilation
4363416899D-mannitol+assimilation
4363416024D-mannose+assimilation
4363427605D-psicose+assimilation
4363416988D-ribose+assimilation
4363433801D-saccharate+assimilation
4363417924D-sorbitol+assimilation
4363416443D-tagatose+assimilation
4363465327D-xylose+assimilation
4363423652dextrin+assimilation
4363417113erythritol+assimilation
4363416537galactarate+assimilation
4363424265gluconate+assimilation
4363417234glucose+assimilation
4363432323glucuronamide+assimilation
4363417754glycerol+assimilation
4363414336glycerol 1-phosphate+assimilation
4363470744glycine-proline+assimilation
4363430849L-arabinose+assimilation
4363418403L-arabitol+assimilation
4363417196L-asparagine+assimilation
4363429991L-aspartate+assimilation
4363418287L-fucose+assimilation
4363429985L-glutamate+assimilation
4363415971L-histidine+assimilation
4363462345L-rhamnose+assimilation
4363417115L-serine+assimilation
4363465328L-xylose+assimilation
4363417306maltose+assimilation
4363461993maltotriose+assimilation
4363429864mannitol+assimilation
4363437684mannose+assimilation
4363474611methyl (R)-lactate+assimilation
4363437657methyl D-glucoside+assimilation
4363451850methyl pyruvate+assimilation
4363417268myo-inositol+assimilation
4363428037N-acetylgalactosamine+assimilation
43634506227N-acetylglucosamine+assimilation
4363473784glycyl-l-glutamate+assimilation
4363418394palatinose+assimilation
4363417309pectin+assimilation
4363417148putrescine+assimilation
4363415361pyruvate+assimilation
4363426490quinate+assimilation
4363417814salicin+assimilation
4363441865sebacic acid+assimilation
4363430031succinate+assimilation
4363417992sucrose+assimilation
4363427082trehalose+assimilation
4363453424tween 20+assimilation
4363453423tween 40+assimilation
4363453426tween 80+assimilation
4363416704uridine+assimilation
4363417151xylitol+assimilation
4363427613amygdalin+builds acid from
4363422599arabinose+builds acid from
4363417234glucose+builds acid from
4363417268myo-inositol+builds acid from
4363429864mannitol+builds acid from
4363426546rhamnose+builds acid from
4363417992sucrose+builds acid from
4363430911sorbitol+builds acid from
4363417234glucose+fermentation
436344853esculin+hydrolysis
4363417632nitrate+reduction
6836830849L-arabinose+fermentation
6836827613amygdalin+fermentation
6836828053melibiose-fermentation
6836817992sucrose+fermentation
6836862345L-rhamnose+fermentation
6836830911sorbitol+fermentation
6836817268myo-inositol+fermentation
6836816899D-mannitol+fermentation
6836817634D-glucose+fermentation
683685291gelatin-hydrolysis
6836827897tryptophan-energy source
6836816199urea-hydrolysis
6836816947citrate-assimilation
6836818257ornithine-degradation
6836825094lysine-degradation
6836829016arginine-hydrolysis

metabolite production

@refChebi-IDmetaboliteproduction
4363415688acetoinno
6836815688acetoinno
6836835581indoleno
6836816136hydrogen sulfideno

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testindole test
4363415688acetoin-
6836815688acetoin-
6836835581indole-

enzymes

@refvalueactivityec
43634alkaline phosphatase+3.1.3.1
43634esterase (C 4)+
43634esterase Lipase (C 8)+
43634lipase (C 14)+
43634leucine arylamidase+3.4.11.1
43634acid phosphatase+3.1.3.2
43634trypsin+3.4.21.4
43634naphthol-AS-BI-phosphohydrolase+
43634beta-galactosidase+3.2.1.23
43634beta-glucuronidase+3.2.1.31
43634beta-glucosidase+3.2.1.21
43634N-acetyl-beta-glucosaminidase+3.2.1.52
43634urease-3.5.1.5
43634catalase-1.11.1.6
68368cytochrome oxidase-1.9.3.1
68368gelatinase-
68368tryptophan deaminase-4.1.99.1
68368urease-3.5.1.5
68368ornithine decarboxylase-4.1.1.17
68368lysine decarboxylase-4.1.1.18
68368arginine dihydrolase-3.5.3.6
68368beta-galactosidase+3.2.1.23

API 20E

@refONPGADH ArgLDC LysODCCITH2SURETDA TrpINDVPGELGLUMANINOSorRHASACMELAMYARAOX
24339+----------++++++-++-

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentlatitudelongitude
24339soil from an ex-landfill sitePuchong, Ayer HitamMalaysiaMYSAsia3.0034101.659
43634an ex-landfill site located in PuchongAyer Hatam, PuchongMalaysiaMYSAsia3.003101.659

isolation source categories

Cat1Cat2Cat3
#Environmental#Terrestrial#Soil
#Engineered#Waste#Landfill

Safety information

risk assessment

  • @ref: 24339
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 24339
  • description: Chania multitudinisentens RB-25 16S ribosomal RNA gene, complete sequence
  • accession: KJ081442
  • length: 1611
  • database: ena
  • NCBI tax ID: 1441930

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Chania multitudinisentens RB-25GCA_000520015completencbi1441930
66792Chania multitudinisentens RB-25GCA_000568195contigncbi1441930
66792Chania multitudinisentens RB-252558860259completeimg1441930
66792Chania multitudinisentens RB-252576861238draftimg1441930

GC content

@refGC-contentmethod
4363450.9genome sequence analysis
2433950.9sequence analysis

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
motileyes93.716no
motileyes93.716no
flagellatedyes55.582no
flagellatedyes55.582no
gram-positiveno98.315no
gram-positiveno98.315no
anaerobicno98.01yes
anaerobicno98.01yes
halophileno90.987no
halophileno90.987no
spore-formingno93.852no
spore-formingno93.852no
thermophileno98.993no
glucose-utilyes96.06yes
glucose-utilyes96.06yes
aerobicyes86.957yes
aerobicyes86.957yes
thermophileno98.993no
glucose-fermentyes91.621no
glucose-fermentyes91.621no

External links

@ref: 24339

culture collection no.: DSM 28811, LMG 28304

straininfo link

  • @ref: 90873
  • straininfo: 397973

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Genetics25975625Complete genome sequence of Serratia multitudinisentens RB-25(T), a novel chitinolytic bacterium.Lim YL, Yong D, Ee R, Tee KK, Yin WF, Chan KGJ Biotechnol10.1016/j.jbiotec.2015.04.0272015Bacterial Proteins/genetics, Chitinases/genetics, *Genome, Bacterial, Molecular Sequence Data, Operon, Sequence Analysis, DNA/*methods, Serratia/enzymology/*genetics/*isolation & purification, Waste Disposal Facilities, Water MicrobiologyTranscriptome
Phylogeny26978486Chania multitudinisentens gen. nov., sp. nov., an N-acyl-homoserine-lactone-producing bacterium in the family Enterobacteriaceae isolated from landfill site soil.Ee R, Madhaiyan M, Ji L, Lim YL, Nor NM, Tee KK, Chen JW, Yin WFInt J Syst Evol Microbiol10.1099/ijsem.0.0010252016Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Enterobacteriaceae/*classification/genetics/isolation & purification, Fatty Acids/chemistry, Genes, Bacterial, Homoserine/*biosynthesis, Lactones/*metabolism, Malaysia, Multilocus Sequence Typing, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Soil Microbiology, Ubiquinone/chemistry, Waste Disposal FacilitiesTranscriptome

Reference

@idauthorstitledoi/urlcataloguejournalpubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
24339Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-28811Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 28811)
43634Robson Ee, Munusamy Madhaiyan, Lianghui Ji, Yan-Lue Lim, Nuruddin Muhammad Nor, Kok-Keng Tee, Jian-Woon Chen, Wai-Fong YinChania multitudinisentens gen. nov., sp. nov., an N-acyl-homoserine-lactone-producing bacterium in the family Enterobacteriaceae isolated from landfill site soil10.1099/ijsem.0.001025IJSEM 66: 2297-2304 201626978486
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
68368Automatically annotated from API 20E
90873Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID397973.1