Name: Chania multitudinisentens RB-25
Creator: BacDive
License: https://creativecommons.org/licenses/by/4.0/

Strain identifier

BacDive ID: 132550

Type strain:

Species: Chania multitudinisentens

Strain Designation: RB-25

Culture col. no.: DSM 28811, LMG 28304

Strain history: <- R. Ee, Univ. of Malaya, Microbiome Lab., Kuala Lumpur, Malaysia; RB-25 <- R. Ee and Lim Yan Lue

NCBI tax ID(s): 1639108 (species)

SI-ID 397973

DSM 28811

Special Collections

Section

When using BacDive for research please cite our paper

Chania multitudinisentens RB-25 is an aerobe, mesophilic, Gram-negative bacterium that was isolated from soil from an ex-landfill site.

Gram-negative
motile
rod-shaped
aerobe
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	09-12-2024 (DD-MM-YYYY)
	Domain	*Bacteria*
	Phylum	*Pseudomonadota*
	Class	*Gammaproteobacteria*
	Order	*Enterobacterales*
	Family	*Yersiniaceae*
	Genus	*Chania*
	Species	**Chania multitudinisentens**
	Full Scientific Name (LPSN)	Chania multitudinisentens Ee et al. 2016

Synonym

"Serratia multitudinisentens"

Information on morphological and physiological properties Morphology

[Ref.: #43634]	Gram stain	negative
[Ref.: #125438]	Gram stain	negative Confidence in %99.25

[Ref.: #43634]	Cell length	7.5-9 µm

[Ref.: #43634]	Cell width	3-4 µm

[Ref.: #43634]	Cell shape	rod-shaped

[Ref.: #43634]	Motility	yes

[Ref.: #43634]	Colony color	Pale white
[Ref.: #43634]	Incubation period	1 day
[Ref.: #43634]	Cultivation medium used	Luria Bertani agar

Multimedia content

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Intellectual property rights:

Information on culture and growth conditions Culture and growth conditions

[Ref.: #43634]

Culture medium

LB (Luria-Bertani) MEDIUM

[Ref.: #43634]

Culture medium growth

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Culture medium

NUTRIENT AGAR (DSMZ Medium 1)

[Ref.: #24339]

Culture medium growth

[Ref.: #24339]

Culture medium link

Medium recipe at MediaDive

[Ref.: #24339]

Culture medium composition

expand / minimize

	Temperatures	Kind of temperature		Temperature
[Ref.: #24339]			growth	28 °C
[Ref.: #43634]			optimum	28 °C

	pH	Kind of pH		pH
[Ref.: #43634]			optimum	5-8

Information on physiology and metabolism Physiology and metabolism

[Ref.: #43634]

Oxygen tolerance

aerobe

[Ref.: #24339]

Name of produced compound

N acyl homoserine lactones

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #43634]		NaCl		optimum	1-8	%(w/v)

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #43634]	(S)-lactate ChEBI	+	assimilation
[Ref.: #43634]	2-deoxyadenosine ChEBI	+	assimilation
[Ref.: #43634]	2-oxoglutarate ChEBI	+	assimilation
[Ref.: #43634]	3-hydroxybutyrate ChEBI	+	assimilation
[Ref.: #43634]	3-O-methyl-D-glucose ChEBI	+	assimilation
[Ref.: #43634]	4-hydroxy-L-proline ChEBI	+	assimilation
[Ref.: #43634]	4-hydroxyphenylacetic acid ChEBI	+	assimilation
[Ref.: #43634]	acetate ChEBI	+	assimilation
[Ref.: #43634]	acetoacetate ChEBI	+	assimilation
[Ref.: #43634]	adenosine ChEBI	+	assimilation
[Ref.: #43634]	alpha-D-glucose ChEBI	+	assimilation
[Ref.: #43634]	amygdalin ChEBI	+	builds acid from
[Ref.: #68368]	amygdalin ChEBI	+	fermentation	* from API 20E
[Ref.: #43634]	arabinose ChEBI	+	assimilation
[Ref.: #43634]	arabinose ChEBI	+	builds acid from
[Ref.: #43634]	arbutin ChEBI	+	assimilation
[Ref.: #68368]	arginine ChEBI	-	hydrolysis	* from API 20E
[Ref.: #43634]	beta-D-allose ChEBI	+	assimilation
[Ref.: #43634]	bromosuccinate ChEBI	+	assimilation
[Ref.: #43634]	cellobiose ChEBI	-	builds acid from
[Ref.: #68368]	citrate ChEBI	-	assimilation	* from API 20E
[Ref.: #43634]	D-arabinose ChEBI	+	assimilation
[Ref.: #43634]	D-arabitol ChEBI	+	assimilation
[Ref.: #43634]	D-fructose ChEBI	+	assimilation
[Ref.: #43634]	D-fructose 6-phosphate ChEBI	+	assimilation
[Ref.: #43634]	D-galactonic acid lactone ChEBI	+	assimilation
[Ref.: #43634]	D-galactose ChEBI	+	assimilation
[Ref.: #43634]	D-galacturonic acid ChEBI	+	assimilation
[Ref.: #43634]	D-gluconate ChEBI	+	assimilation
[Ref.: #43634]	D-glucosamine ChEBI	+	assimilation
[Ref.: #68368]	D-glucose ChEBI	+	fermentation	* from API 20E
[Ref.: #43634]	D-glucose 6-phosphate ChEBI	+	assimilation
[Ref.: #43634]	D-glucuronate ChEBI	+	assimilation
[Ref.: #43634]	D-mannitol ChEBI	+	assimilation
[Ref.: #68368]	D-mannitol ChEBI	+	fermentation	* from API 20E
[Ref.: #43634]	D-mannose ChEBI	+	assimilation
[Ref.: #43634]	D-psicose ChEBI	+	assimilation
[Ref.: #43634]	D-ribose ChEBI	+	assimilation
[Ref.: #43634]	D-saccharate ChEBI	+	assimilation
[Ref.: #43634]	D-sorbitol ChEBI	+	assimilation
[Ref.: #43634]	D-tagatose ChEBI	+	assimilation
[Ref.: #43634]	D-xylose ChEBI	+	assimilation
[Ref.: #43634]	dextrin ChEBI	+	assimilation
[Ref.: #43634]	erythritol ChEBI	+	assimilation
[Ref.: #43634]	esculin ChEBI	+	hydrolysis
[Ref.: #43634]	galactarate ChEBI	+	assimilation
[Ref.: #68368]	gelatin ChEBI	-	hydrolysis	* from API 20E
[Ref.: #43634]	gluconate ChEBI	+	assimilation
[Ref.: #43634]	glucose ChEBI	+	assimilation
[Ref.: #43634]	glucose ChEBI	+	builds acid from
[Ref.: #43634]	glucose ChEBI	+	fermentation
[Ref.: #43634]	glucuronamide ChEBI	+	assimilation
[Ref.: #43634]	glycerol ChEBI	+	assimilation
[Ref.: #43634]	glycerol 1-phosphate ChEBI	+	assimilation
[Ref.: #43634]	glycine-proline ChEBI	+	assimilation
[Ref.: #43634]	glycyl-l-glutamate ChEBI	+	assimilation
[Ref.: #43634]	L-arabinose ChEBI	+	assimilation
[Ref.: #68368]	L-arabinose ChEBI	+	fermentation	* from API 20E
[Ref.: #43634]	L-arabitol ChEBI	+	assimilation
[Ref.: #43634]	L-asparagine ChEBI	+	assimilation
[Ref.: #43634]	L-aspartate ChEBI	+	assimilation
[Ref.: #43634]	L-fucose ChEBI	+	assimilation
[Ref.: #43634]	L-glutamate ChEBI	+	assimilation
[Ref.: #43634]	L-histidine ChEBI	+	assimilation
[Ref.: #43634]	L-malate ChEBI	+	assimilation
[Ref.: #43634]	L-rhamnose ChEBI	+	assimilation
[Ref.: #68368]	L-rhamnose ChEBI	+	fermentation	* from API 20E
[Ref.: #43634]	L-serine ChEBI	+	assimilation
[Ref.: #43634]	L-xylose ChEBI	+	assimilation
[Ref.: #68368]	lysine ChEBI	-	degradation	* from API 20E
[Ref.: #43634]	maltose ChEBI	+	assimilation
[Ref.: #43634]	maltotriose ChEBI	+	assimilation
[Ref.: #43634]	mannitol ChEBI	+	assimilation
[Ref.: #43634]	mannitol ChEBI	+	builds acid from
[Ref.: #43634]	mannose ChEBI	+	assimilation
[Ref.: #43634]	melibiose ChEBI	-	builds acid from
[Ref.: #68368]	melibiose ChEBI	-	fermentation	* from API 20E
[Ref.: #43634]	methyl (R)-lactate ChEBI	+	assimilation
[Ref.: #43634]	methyl D-glucoside ChEBI	+	assimilation
[Ref.: #43634]	methyl pyruvate ChEBI	+	assimilation
[Ref.: #43634]	myo-inositol ChEBI	+	assimilation
[Ref.: #43634]	myo-inositol ChEBI	+	builds acid from
[Ref.: #68368]	myo-inositol ChEBI	+	fermentation	* from API 20E
[Ref.: #43634]	N-acetylgalactosamine ChEBI	+	assimilation
[Ref.: #43634]	N-acetylglucosamine ChEBI	+	assimilation
[Ref.: #43634]	nitrate ChEBI	+	reduction
[Ref.: #68368]	ornithine ChEBI	-	degradation	* from API 20E
[Ref.: #43634]	palatinose ChEBI	+	assimilation
[Ref.: #43634]	pectin ChEBI	+	assimilation
[Ref.: #43634]	putrescine ChEBI	+	assimilation
[Ref.: #43634]	pyruvate ChEBI	+	assimilation
[Ref.: #43634]	quinate ChEBI	+	assimilation
[Ref.: #43634]	rhamnose ChEBI	+	builds acid from
[Ref.: #43634]	ribitol ChEBI	+	assimilation
[Ref.: #43634]	salicin ChEBI	+	assimilation
[Ref.: #43634]	sebacic acid ChEBI	+	assimilation
[Ref.: #43634]	sorbitol ChEBI	+	builds acid from
[Ref.: #68368]	sorbitol ChEBI	+	fermentation	* from API 20E
[Ref.: #43634]	succinate ChEBI	+	assimilation
[Ref.: #43634]	sucrose ChEBI	+	assimilation
[Ref.: #43634]	sucrose ChEBI	+	builds acid from
[Ref.: #68368]	sucrose ChEBI	+	fermentation	* from API 20E
[Ref.: #43634]	trehalose ChEBI	+	assimilation
[Ref.: #68368]	tryptophan ChEBI	-	energy source	* from API 20E
[Ref.: #43634]	tween 20 ChEBI	+	assimilation
[Ref.: #43634]	tween 40 ChEBI	+	assimilation
[Ref.: #43634]	tween 80 ChEBI	+	assimilation
[Ref.: #68368]	urea ChEBI	-	hydrolysis	* from API 20E
[Ref.: #43634]	uridine ChEBI	+	assimilation
[Ref.: #43634]	xylitol ChEBI	+	assimilation
	Only first 10 entries are displayed. Click here to see all.

	Metabolite	Production
[Ref.: #43634]	acetoin ChEBI	no
[Ref.: #68368]	acetoin ChEBI	no	* from API 20E
[Ref.: #68368]	hydrogen sulfide ChEBI	no	* from API 20E
[Ref.: #68368]	indole ChEBI	no	* from API 20E

	Metabolite	Voges-Proskauer-test	Indole test
[Ref.: #43634]	acetoin ChEBI	-
[Ref.: #68368]	acetoin ChEBI	-		* from API 20E
[Ref.: #68368]	indole ChEBI		-	* from API 20E

	Enzyme	Enzyme activity	EC number
[Ref.: #43634]	acid phosphatase	+	3.1.3.2
[Ref.: #43634]	alkaline phosphatase	+	3.1.3.1
[Ref.: #68368]	arginine dihydrolase	-	3.5.3.6	* from API 20E
[Ref.: #43634]	beta-galactosidase	+	3.2.1.23
[Ref.: #68368]	beta-galactosidase	+	3.2.1.23	* from API 20E
[Ref.: #43634]	beta-glucosidase	+	3.2.1.21
[Ref.: #43634]	beta-glucuronidase	+	3.2.1.31
[Ref.: #43634]	catalase	-	1.11.1.6
[Ref.: #68368]	cytochrome oxidase	-	1.9.3.1	* from API 20E
[Ref.: #43634]	esterase (C 4)	+
[Ref.: #43634]	esterase Lipase (C 8)	+
[Ref.: #68368]	gelatinase	-		* from API 20E
[Ref.: #43634]	leucine arylamidase	+	3.4.11.1
[Ref.: #43634]	lipase (C 14)	+
[Ref.: #68368]	lysine decarboxylase	-	4.1.1.18	* from API 20E
[Ref.: #43634]	N-acetyl-beta-glucosaminidase	+	3.2.1.52
[Ref.: #43634]	naphthol-AS-BI-phosphohydrolase	+
[Ref.: #68368]	ornithine decarboxylase	-	4.1.1.17	* from API 20E
[Ref.: #43634]	trypsin	+	3.4.21.4
[Ref.: #68368]	tryptophan deaminase	-	4.1.99.1	* from API 20E
[Ref.: #43634]	urease	-	3.5.1.5
[Ref.: #68368]	urease	-	3.5.1.5	* from API 20E
	Only first 10 entries are displayed. Click here to see all.

API® 20E:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	beta-GalactosidaseONPG	Arginine dihydrolaseADH (Arg)	Lysine decarboxylaseLDC (Lys)	Ornithine decarboxylaseODC	Citrate utilizationCIT	H2S productionH2S	Urease/urea hydrolysisURE	Tryptophan deaminaseTDA (Trp)	Indole productionIND	Acetoin production (Voges Proskauer test)VP	Gelatinase/gelatin hydrolysisGEL	Fermentation/oxidation of D-glucoseGLU	Fermentation/oxidation of D-mannitolMAN	Fermentation/oxidation of inositolINO	Fermentation/oxidation of sorbitolSor	Fermentation/oxidation of L-rhamnoseRHA	Fermentation/oxidation of sucroseSAC	Fermentation/oxidation of melibioseMEL	Fermentation/oxidation of amygdalinAMY	Fermentation/oxidation of L-arabinoseARA	Cytochrome oxidaseOX	Nitrite productionNO2	Reduction to N2N2	MotilityMOB	Growth on MacConkey mediumMAC	Oxidative catabolisation of D-glucoseOF-O	Fermentation of D-glucoseOF-F
[Ref.: #24339]	18370	+	-	-	-	-	-	-	-	-	-	-	+	+	+	+	+	+	-	+	+	-	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #24339]	Sample type/isolated from	soil from an ex-landfill site
[Ref.: #24339]	Geographic location (country and/or sea, region)	Puchong, Ayer Hitam
[Ref.: #24339]	Country	Malaysia
[Ref.: #24339]	Country ISO 3 Code	MYS
[Ref.: #24339]	Continent	Asia
[Ref.: #24339]	Geographic location	3.0034°/101.6590°

[Ref.: #43634]	Sample type/isolated from	an ex-landfill site located in Puchong
[Ref.: #43634]	Geographic location (country and/or sea, region)	Ayer Hatam, Puchong
[Ref.: #43634]	Country	Malaysia
[Ref.: #43634]	Country ISO 3 Code	MYS
[Ref.: #43634]	Continent	Asia
[Ref.: #43634]	Geographic location	3.0030°/101.6590°
* marker position based on {}

Isolation sources categories

#Environmental	#Terrestrial	#Soil
#Engineered	#Waste	#Landfill

What are isolation sources categories?

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #24339]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #24339]	Chania multitudinisentens RB-25 16S ribosomal RNA gene, complete sequence	KJ081442	1611	GenBank ENA	1441930 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Chania multitudinisentens RB-25	GCA_000520015	complete	GenBank ENA	1441930 tax ID	*
[Ref.: #66792]	Chania multitudinisentens RB-25	GCA_000568195	contig	GenBank ENA	1441930 tax ID	*
[Ref.: #66792]	Chania multitudinisentens RB-25	2558860259	complete	JGI IMG/M	1441930 tax ID	*
[Ref.: #66792]	Chania multitudinisentens RB-25	2576861238	draft	JGI IMG/M	1441930 tax ID	*

[Ref.: #43634]	GC-content	50.9 mol%	genome sequence analysis
[Ref.: #24339]	GC-content	50.9 mol%	sequence analysis

Data predicted using genome information as a basis Genome-based predictions

	Model	Trait	Prediction	Confidence in %	In training data
	Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Chania multitudinisentens RB-25 RB-25 NCBI: GCA_000520015.2
[Ref.: #125438]	gram-positive	gram-positiveⓘ	no	99.25	yes
[Ref.: #125438]	anaerobic	anaerobicⓘ	no	95.495	yes
[Ref.: #125438]	aerobic	aerobicⓘ	no	64.923	yes
[Ref.: #125438]	spore-forming	spore-formingⓘ	no	86.061	no
[Ref.: #125438]	thermophile	thermophilicⓘ	no	98.25	yes
[Ref.: #125438]	motile2+	flagellatedⓘ	yes	78.16	no

	Model	Trait	Prediction	Confidence in %
	Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Chania multitudinisentens RB-25 IMG: 2558860259
[Ref.: #125439]	BacteriaNet	spore_formationⓘ	no	81.2
[Ref.: #125439]	BacteriaNet	motilityⓘ	yes	63
[Ref.: #125439]	BacteriaNet	gram_stainⓘ	negative	81.2
[Ref.: #125439]	BacteriaNet	oxygen_toleranceⓘ	facultative aerobe	55.8

Availability in culture collections External links

[Ref.: #24339]

Culture collection no.

DSM 28811, LMG 28304

[Ref.: #90873]

SI-ID 397973

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Chania multitudinisentens gen. nov., sp. nov., an N-acyl-homoserine-lactone-producing bacterium in the family Enterobacteriaceae isolated from landfill site soil.	Ee R, Madhaiyan M, Ji L, Lim YL, Nor NM, Tee KK, Chen JW, Yin WF	Int J Syst Evol Microbiol	10.1099/ijsem.0.001025	2016	*
Genetics	Complete genome sequence of Serratia multitudinisentens RB-25(T), a novel chitinolytic bacterium.	Lim YL, Yong D, Ee R, Tee KK, Yin WF, Chan KG	J Biotechnol	10.1016/j.jbiotec.2015.04.027	2015	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#24339

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 28811

#43634

Robson Ee, Munusamy Madhaiyan, Lianghui Ji, Yan-Lue Lim, Nuruddin Muhammad Nor, Kok-Keng Tee, Jian-Woon Chen, Wai-Fong Yin: Chania multitudinisentens gen. nov., sp. nov., an N-acyl-homoserine-lactone-producing bacterium in the family Enterobacteriaceae isolated from landfill site soil. IJSEM 66: 2297 - 2304 2016 ( DOI 10.1099/ijsem.0.001025 , PubMed 26978486 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#68368

Automatically annotated from API 20E .

#90873

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID397973.1 )

#125438

Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )

#125439

Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .

* These data were automatically processed and therefore are not curated

Change proposal

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Chania multitudinisentens RB-25 RB-25

Chania multitudinisentens RB-25

Change proposal

Change proposal