Strain identifier
BacDive ID: 132541
Type strain: 
Species: Bacillus mesophilus
Strain Designation: SA4
Strain history: <- G. Liu, Fujian Academy of Agricultural Sciences, Fuzhou, China; SA4
NCBI tax ID(s): 1808955 (species)
version 9.1 (current version)
General
@ref: 24330
BacDive-ID: 132541
DSM-Number: 101000
keywords: genome sequence, 16S sequence, Bacteria, spore-forming, motile
description: Bacillus mesophilus SA4 is a spore-forming, motile bacterium that was isolated from soil from an abandoned marine solar saltern.
NCBI tax id
- NCBI tax id: 1808955
- Matching level: species
strain history
- @ref: 24330
- history: <- G. Liu, Fujian Academy of Agricultural Sciences, Fuzhou, China; SA4
doi: 10.13145/bacdive132541.20240916.9.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/bacillota
- domain: Bacteria
- phylum: Bacillota
- class: Bacilli
- order: Caryophanales
- family: Bacillaceae
- genus: Bacillus
- species: Bacillus mesophilus
- full scientific name: Bacillus mesophilus Zhou et al. 2017
@ref: 24330
domain: Bacteria
phylum: Firmicutes
class: Bacilli
order: Bacillales
family: Bacillaceae
genus: Bacillus
species: Bacillus mesophilus
full scientific name: Bacillus mesophilus Zhou et al. 2017
strain designation: SA4
type strain: yes
Culture and growth conditions
culture medium
- @ref: 24330
- name: BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514)
- growth: yes
- link: https://mediadive.dsmz.de/medium/514
- composition: Name: BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514) Composition: NaCl 19.45 g/l MgCl2 5.9 g/l Bacto peptone 5.0 g/l Na2SO4 3.24 g/l CaCl2 1.8 g/l Yeast extract 1.0 g/l KCl 0.55 g/l NaHCO3 0.16 g/l Fe(III) citrate 0.1 g/l KBr 0.08 g/l SrCl2 0.034 g/l H3BO3 0.022 g/l Na2HPO4 0.008 g/l Na-silicate 0.004 g/l NaF 0.0024 g/l (NH4)NO3 0.0016 g/l Distilled water
culture temp
- @ref: 24330
- growth: positive
- type: growth
- temperature: 30
Physiology and metabolism
spore formation
| @ref | spore formation | confidence |
|---|---|---|
| 69480 | yes | 95.029 |
| 69481 | yes | 100 |
Isolation, sampling and environmental information
isolation
- @ref: 24330
- sample type: soil from an abandoned marine solar saltern
- geographic location: Shandong Province, Wendeng (122° 0' 21.59'' E, 36° 69' 52.82'' N)
- country: China
- origin.country: CHN
- continent: Asia
- latitude: 38.6172
- longitude: 122.6
isolation source categories
| Cat1 | Cat2 | Cat3 |
|---|---|---|
| #Environmental | #Aquatic | #Marine |
| #Environmental | #Terrestrial | #Soil |
| #Condition | #Saline |
Safety information
risk assessment
- @ref: 24330
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 24330
- description: Bacillus mesophilus strain SA4 16S ribosomal RNA gene, partial sequence
- accession: KU324461
- length: 1501
- database: nuccore
- NCBI tax ID: 1808955
Genome sequences
| @ref | description | accession | assembly level | database | NCBI tax ID |
|---|---|---|---|---|---|
| 66792 | Bacillus mesophilus DSM 101000 | GCA_016908955 | contig | ncbi | 1808955 |
| 66792 | Bacillus mesophilus SA4 | GCA_011008845 | contig | ncbi | 1808955 |
| 66792 | Bacillus mesophilus strain DSM 101000 | 1808955.6 | wgs | patric | 1808955 |
| 66792 | Bacillus mesophilus DSM 101000 | 2901236122 | draft | img | 1808955 |
GC content
- @ref: 24330
- GC-content: 42.4
- method: high performance liquid chromatography (HPLC)
Genome-based predictions
predictions
| @ref | trait | model | description | prediction | confidence | training_data |
|---|---|---|---|---|---|---|
| 69481 | spore-forming | spore-forming | Ability to form endo- or exospores | yes | 100 | no |
| 69480 | gram-positive | gram-positive | Positive reaction to Gram-staining | yes | 72.227 | no |
| 69480 | anaerobic | anaerobic | Ability to grow under anoxygenic conditions (including facultative anaerobes) | no | 90.363 | no |
| 69480 | spore-forming | spore-forming | Ability to form endo- or exospores | yes | 95.029 | no |
| 69480 | aerobic | aerobic | Ability to grow under oxygenic conditions (including facultative aerobes) | yes | 66.18 | no |
| 69480 | thermophilic | thermophile | Ability to grow at temperatures above or equal to 45°C | no | 89.268 | no |
| 69480 | flagellated | motile2+ | Ability to perform flagellated movement | yes | 88.117 | no |
External links
@ref: 24330
culture collection no.: DSM 101000, CCTCC AB 2015209
straininfo link
- @ref: 90866
- straininfo: 402642
literature
- topic: Metabolism
- Pubmed-ID: 27084709
- title: Bacillus mesophilus sp. nov., an alginate-degrading bacterium isolated from a soil sample collected from an abandoned marine solar saltern.
- authors: Zhou YX, Liu GH, Liu B, Chen GJ, Du ZJ
- journal: Antonie Van Leeuwenhoek
- DOI: 10.1007/s10482-016-0692-7
- year: 2016
- mesh: Alginates/*metabolism, Bacillus/genetics/*isolation & purification/*metabolism, Cell Wall/metabolism, China, Fatty Acids/metabolism, Genotype, Glucuronic Acid/metabolism, Hexuronic Acids/metabolism, Nucleic Acid Hybridization, Peptidoglycan/metabolism, Phenotype, Phosphatidylethanolamines/metabolism, Phylogeny, Sequence Analysis, DNA, Sodium Chloride, Soil/chemistry, *Soil Microbiology, Vitamin K 2/metabolism
- topic2: Phylogeny
Reference
| @id | authors | title | doi/url | catalogue |
|---|---|---|---|---|
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | 10.1099/ijsem.0.004332 | |
| 24330 | Curators of the DSMZ | https://www.dsmz.de/collection/catalogue/details/culture/DSM-101000 | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 101000) | |
| 66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | https://diaspora-project.de/progress.html#genomes | |
| 69480 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | https://diaspora-project.de/progress.html#genomes | |
| 69481 | Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch | Predictions based on the model GenomeNet Sporulation v. 1 | 10.21203/rs.3.rs-2527258/v1 | |
| 90866 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | StrainInfo: A central database for resolving microbial strain identifiers | 10.60712/SI-ID402642.1 |