Strain identifier

BacDive ID: 132476

Type strain: Yes

Species: Streptomyces humi

Strain history: <- L.-H. Lee, Monash University Malaya, Malaysia; MUSC 119

NCBI tax ID(s): 1428620 (species)

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General

@ref: 24265

BacDive-ID: 132476

DSM-Number: 42174

keywords: 16S sequence, Bacteria, mesophilic

description: Streptomyces humi DSM 42174 is a mesophilic bacterium that builds an aerial mycelium and was isolated from mangrove sediment.

NCBI tax id

  • NCBI tax id: 1428620
  • Matching level: species

strain history

  • @ref: 24265
  • history: <- L.-H. Lee, Monash University Malaya, Malaysia; MUSC 119

doi: 10.13145/bacdive132476.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Kitasatosporales
  • family: Streptomycetaceae
  • genus: Streptomyces
  • species: Streptomyces humi
  • full scientific name: Streptomyces humi Zainal et al. 2016

@ref: 24265

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Streptomycetaceae

genus: Streptomyces

species: Streptomyces humi

full scientific name: Streptomyces humi Zainal et al. 2016

type strain: yes

Morphology

colony morphology

@refcolony colormedium used
69354Grey beige (1019)ISP 6
69354Grey beige (1019)suter without tyrosine
69354Clay brown (8003)ISP 2
69354Brown beige (1011)ISP 3
69354Brown beige (1011)ISP 4
69354Nut brown (8011)ISP 5
69354Sepia brown (8014)ISP 7
69354Umbra grey (7022)suter with tyrosine

multicellular morphology

@refforms multicellular complexcomplex namecomplex colormedium name
69354yesAerial myceliumStone grey (7030)ISP 2
69354yesAerial myceliumStone grey (7030)ISP 3
69354yesAerial myceliumLight ivory (1015)ISP 4
69354yesAerial myceliumStone grey (7030)ISP 5
69354noAerial myceliumISP 6
69354yesAerial myceliumGrey beige (1019)ISP 7
69354noAerial myceliumsuter with tyrosine
69354noAerial myceliumsuter without tyrosine

pigmentation

@refproductionnamecolor
69354yesMelanin
69354yessoluble pigmentOchre brown (8001), copper brown (8004), brown beige (1011), clay brown (8003), olive brown (8008), fawn brown (8007), jet black (9005)

multimedia

@refmultimedia contentcaptionintellectual property rights
24265https://www.dsmz.de/microorganisms/photos/DSM_42174.jpgMedium 65 28°C© Leibniz-Institut DSMZ
69354DSM_42174_image3.jpegPlates (65, ISP2, ISP3, ISP4, ISP5, ISP7)Helmholtz-Zentrum für Infektionsforschung GmbH
69354DSM_42174_image4.jpegPlates (65, ISP2, ISP3, ISP4, ISP5, ISP7)Helmholtz-Zentrum für Infektionsforschung GmbH

Culture and growth conditions

culture medium

  • @ref: 24265
  • name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/65
  • composition: Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water

culture temp

  • @ref: 24265
  • growth: positive
  • type: growth
  • temperature: 28
  • range: mesophilic

Physiology and metabolism

halophily

  • @ref: 69354
  • salt: NaCl
  • growth: positive
  • tested relation: growth
  • concentration: 0-5 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6935422599arabinose+growth
6935462968cellulose-growth
6935428757fructose+growth
6935417234glucose+growth
6935417268inositol+growth
6935437684mannose+growth
6935416634raffinose+growth
6935426546rhamnose+growth
6935417992sucrose+growth
6935418222xylose+growth
6837917632nitrate-reduction
683794853esculin+hydrolysis
6837916199urea-hydrolysis
683795291gelatin+hydrolysis
6837917634D-glucose-fermentation
6837916988D-ribose-fermentation
6837965327D-xylose-fermentation
6837916899D-mannitol-fermentation
6837917306maltose-fermentation
6837917716lactose-fermentation
6837917992sucrose-fermentation
6837928087glycogen-fermentation

enzymes

@refvalueactivityec
68379gelatinase+
68379urease-3.5.1.5
68379beta-glucosidase+3.2.1.21
68379N-acetyl-beta-glucosaminidase-3.2.1.52
68379alpha-glucosidase-3.2.1.20
68379beta-galactosidase+3.2.1.23
68379beta-glucuronidase-3.2.1.31
68379alkaline phosphatase+3.1.3.1
68379pyrrolidonyl arylamidase-3.4.19.3
68379pyrazinamidase-3.5.1.B15
68382alkaline phosphatase+3.1.3.1
68382esterase lipase (C 8)+
68382leucine arylamidase+3.4.11.1
68382valine arylamidase+
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382beta-galactosidase+3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382beta-glucosidase+3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase+3.2.1.24

API coryne

@refNITPYZPYRAPALbeta GURbeta GALalpha GLUbeta NAGESCUREGELGLURIBXYLMANMALLACSACGLYG
69354---+-+--+-+--------

API zym

@refAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
69354++/-++/-+++/---+++/-+-+/-+-++/-

Isolation, sampling and environmental information

isolation

  • @ref: 24265
  • sample type: mangrove sediment
  • geographic location: Tanjung Lumpur in the State of Pahang (3° 48' 3.2'' N, 103° 20' 11.0''E)
  • country: Malaysia
  • origin.country: MYS
  • continent: Asia
  • latitude: 3.8009
  • longitude: 103.336

isolation source categories

Cat1Cat2Cat3
#Environmental#Aquatic#Mangrove
#Environmental#Aquatic#Sediment
#Host#Plants#Tree

Sequence information

16S sequences

  • @ref: 24265
  • description: Streptomyces humi strain MUSC 119 16S ribosomal RNA gene, partial sequence
  • accession: KJ632661
  • length: 1488
  • database: ena
  • NCBI tax ID: 1428620

GC content

  • @ref: 24265
  • GC-content: 72.6
  • method: high performance liquid chromatography (HPLC)

External links

@ref: 24265

culture collection no.: DSM 42174, MCCC 1K00505, MUSC 119

literature

  • topic: Phylogeny
  • Pubmed-ID: 26786500
  • title: Streptomyces humi sp. nov., an actinobacterium isolated from soil of a mangrove forest.
  • authors: Zainal N, Ser HL, Yin WF, Tee KK, Lee LH, Chan KG
  • journal: Antonie Van Leeuwenhoek
  • DOI: 10.1007/s10482-016-0653-1
  • year: 2016
  • mesh: Bacterial Typing Techniques, Base Composition, *Forests, Genome, Bacterial, Genomics, Phenotype, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Soil Microbiology, Streptomyces/*classification/isolation & purification
  • topic2: Genetics

Reference

@idauthorstitledoi/urlcatalogue
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
24265Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-42174Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 42174)
68379Automatically annotated from API Coryne
68382Automatically annotated from API zym
69354Wink, J.Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweighttps://cdn.dsmz.de/wink/DSM%2042174.pdf