Strain identifier

BacDive ID: 132416

Type strain: Yes

Species: Athalassotoga saccharophila

Strain Designation: NAS-01

Strain history: T. Itoh; RIKEN BRC-JCM, Japan; NAS-01.

NCBI tax ID(s): 1441386 (species)

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General

@ref: 24205

BacDive-ID: 132416

DSM-Number: 28954

keywords: genome sequence, 16S sequence, Bacteria, anaerobe, Gram-negative, rod-shaped

description: Athalassotoga saccharophila NAS-01 is an anaerobe, Gram-negative, rod-shaped bacterium that was isolated from low-salt acidic terrestrial hot spring.

NCBI tax id

  • NCBI tax id: 1441386
  • Matching level: species

strain history

@refhistory
24205<- T. Itoh, JCM, RIKEN BioResource Center; NAS-01 <- M. Onishi and T. Itoh
67770T. Itoh; RIKEN BRC-JCM, Japan; NAS-01.

doi: 10.13145/bacdive132416.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/thermotogota
  • domain: Bacteria
  • phylum: Thermotogota
  • class: Thermotogae
  • order: Mesoaciditogales
  • family: Mesoaciditogaceae
  • genus: Athalassotoga
  • species: Athalassotoga saccharophila
  • full scientific name: Athalassotoga saccharophila Itoh et al. 2016

@ref: 24205

domain: Bacteria

phylum: Thermotogae

class: Thermotogae

order: Mesoaciditogales

family: Mesoaciditogaceae

genus: Athalassotoga

species: Athalassotoga saccharophila

full scientific name: Athalassotoga saccharophila Itoh et al. 2016

strain designation: NAS-01

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell widthcell shapemotilityconfidence
44023negative0.8-2.0 µm0.7-0.8 µmrod-shapedno
69480negative99.886

Culture and growth conditions

culture medium

  • @ref: 24205
  • name: ATHALASSOTOGA MEDIUM (DSMZ Medium 1604)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/1604
  • composition: Name: ATHALASSOTOGA MEDIUM (DSMZ Medium 1604) Composition: KH2PO4 2.96736 g/l Fe(III) citrate 2.37389 g/l MgSO4 x 7 H2O 0.49456 g/l Yeast extract 0.49456 g/l FeCl2 x 4 H2O 0.257171 g/l CaCl2 x 2 H2O 0.24728 g/l (NH4)2SO4 0.197824 g/l L-Cysteine HCl x H2O 0.0395648 g/l Na2B4O7 x 10 H2O 0.00445104 g/l MnCl2 x 4 H2O 0.00178042 g/l ZnSO4 x 7 H2O 0.000217606 g/l Pyridoxine hydrochloride 9.8912e-05 g/l (DL)-alpha-Lipoic acid 4.9456e-05 g/l p-Aminobenzoic acid 4.9456e-05 g/l Nicotinic acid 4.9456e-05 g/l Riboflavin 4.9456e-05 g/l Thiamine HCl 4.9456e-05 g/l Calcium D-(+)-pantothenate 4.9456e-05 g/l CuCl2 x 2 H2O 4.9456e-05 g/l VOSO4 x 2 H2O 2.96736e-05 g/l Na2MoO4 x 2 H2O 2.96736e-05 g/l Folic acid 1.97824e-05 g/l Biotin 1.97824e-05 g/l CoSO4 x 7 H2O 9.8912e-06 g/l Vitamin B12 9.8912e-07 g/l Distilled water

culture temp

@refgrowthtypetemperaturerangeconfidence
44023positivegrowth30-60
44023positiveoptimum55thermophilic
24205positivegrowth55thermophilic
67770positivegrowth55thermophilic
69480thermophilic99.746

culture pH

@refabilitytypepH
44023positivegrowth4.5-7.5
44023positiveoptimum5.5-6.0

Physiology and metabolism

oxygen tolerance

@refoxygen toleranceconfidence
44023anaerobe
69480anaerobe100

spore formation

@refspore formationconfidence
44023no
69480no99.986

halophily

@refsaltgrowthtested relationconcentrationhalophily level
44023NaClpositivegrowth0-1 %
44023NaClpositiveoptimum0 %
44023non-halophilic

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
4402330089acetate-carbon source
4402317968butyrate-carbon source
44023casamino acids-carbon source
4402316947citrate-carbon source
4402315740formate-carbon source
4402329806fumarate-carbon source
4402328260galactose-carbon source
4402324996lactate-carbon source
4402325115malate-carbon source
4402317272propionate-carbon source
4402315361pyruvate-carbon source
4402330031succinate-carbon source
4402330089acetate-energy source
4402317968butyrate-energy source
44023casamino acids-energy source
4402316947citrate-energy source
4402315740formate-energy source
4402329806fumarate-energy source
4402328260galactose-energy source
4402324996lactate-energy source
4402325115malate-energy source
4402317272propionate-energy source
4402315361pyruvate-energy source
4402330031succinate-energy source
4402315824D-fructose+carbon source
4402317634D-glucose+carbon source
4402316024D-mannose+carbon source
4402365327D-xylose+carbon source
4402328087glycogen+carbon source
4402330849L-arabinose+carbon source
4402317716lactose+carbon source
4402317306maltose+carbon source
44023peptone+carbon source
4402333942ribose+carbon source
4402328017starch+carbon source
4402317992sucrose+carbon source
44023yeast extract+carbon source
4402329034ferric iron+electron acceptor
4402316283L-cystine+electron acceptor
4402316094thiosulfate+electron acceptor
4402315824D-fructose+energy source
4402317634D-glucose+energy source
4402316024D-mannose+energy source
4402365327D-xylose+energy source
4402328087glycogen+energy source
4402330849L-arabinose+energy source
4402317716lactose+energy source
4402317306maltose+energy source
44023peptone+energy source
4402333942ribose+energy source
4402328017starch+energy source
4402317992sucrose+energy source
44023yeast extract+energy source

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentlatitudelongitudesampling dateenrichment culture compositionenrichment culture durationenrichment culture temperatureisolation procedure
24205low-salt acidic terrestrial hot springTochigi prefecture, OkushiobaraJapanJPNAsia36.9547139.78
44023acidic hot spring waterOku-Shiobara, TochigiJapanJPNAsia36.9547139.782012-040.2g (NH 4)2 SO4, 3.0?g KH2PO4, 0.5?g MgSO 4?.?7H2O, 0.25?g CaCl2?.?2H2O, 1.8?mg MnCl2?.?4H2O, 4.5?mg Na2B4O7?.?10H2O, 0.22?mg ZnSO4?.7H2O, 0.05?mg CuCl2?.?2H2O, 0.03?mg Na2MoO4?.?2H2O, 0.03?mg VOSO4?.?nH2O, 0.01?mg CoSO4?.?7H2O, 0.5?g yeast extract (BD-Difco), 10?mM Fe(III)citrate and 10.0?ml vitamin mixture of Balch et al. (1979); pH adjusted to 5.02 weeks55The medium was placed in culture bottles under a gas phase of N2/CO2 (4 : 1, v/v), and reduced with 0.25 mM L-cysteine.HCl and 1.3 mM FeCl2 . 4H2O
67770Low-salt acidic terrestrial hot spring in Oku-ShiobaraTochigiJapanJPNAsia

isolation source categories

Cat1Cat2Cat3
#Environmental#Terrestrial
#Condition#Thermophilic (>45°C)
#Condition#Acidic
#Environmental#Aquatic#Thermal spring

Safety information

risk assessment

  • @ref: 24205
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 24205
  • description: Thermotogales bacterium NAS-01 gene for 16S ribosomal RNA, partial sequence
  • accession: AB898788
  • length: 1500
  • database: ena
  • NCBI tax ID: 1441386

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Athalassotoga saccharophila NAS-01GCA_009936215completencbi1441386
66792Athalassotoga saccharophila strain NAS-011441386.9wgspatric1441386
66792Athalassotoga saccharophila NAS-012883451585completeimg1441386

GC content

  • @ref: 24205
  • GC-content: 41.1
  • method: high performance liquid chromatography (HPLC)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno80no
motileyes72.662yes
flagellatedno94.133no
gram-positiveno94.72no
anaerobicyes99.06yes
aerobicno97.926no
halophileno89.656yes
spore-formingno90.383no
glucose-utilyes91.303yes
thermophileyes100no
glucose-fermentyes56.794no

External links

@ref: 24205

culture collection no.: DSM 28954, JCM 19762

straininfo link

  • @ref: 90756
  • straininfo: 401869

literature

topicPubmed-IDtitleauthorsjournalDOIyear
Phylogeny26651491Athalassotoga saccharophila gen. nov., sp. nov., isolated from an acidic terrestrial hot spring, and proposal of Mesoaciditogales ord. nov. and Mesoaciditogaceae fam. nov. in the phylum Thermotogae.Itoh T, Onishi M, Kato S, Iino T, Sakamoto M, Kudo T, Takashina T, Ohkuma MInt J Syst Evol Microbiol10.1099/ijsem.0.0008332015
Genetics32299888Complete Genome Sequence of Athalassotoga saccharophila Strain NAS-01, a Deep-Branching Thermophilic Lineage in the Phylum Thermotogae.Kato S, Itoh T, Ohkuma MMicrobiol Resour Announc10.1128/MRA.00322-202020

Reference

@idauthorstitledoi/urlcataloguejournalpubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
24205Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-28954Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 28954)
44023Takashi Itoh, Masafumi Onishi, Shingo Kato, Takao Iino, Mitsuo Sakamoto, Takuji Kudo, Tomonori Takashina and Moriya OhkumaAthalassotoga saccharophila gen. nov., sp. nov., isolated from an acidic terrestrial hot spring, and proposal of Mesoaciditogales ord. nov. and Mesoaciditogaceae fam. nov. in the phylum Thermotogae10.1099/ijsem.0.000833IJSEM 66: 1045-1051 201626651491
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
90756Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID401869.1