Name: Athalassotoga saccharophila NAS-01
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 132416

Type strain:

Species: Athalassotoga saccharophila

Strain Designation: NAS-01

Culture col. no.: DSM 28954, JCM 19762

Strain history: T. Itoh; RIKEN BRC-JCM, Japan; NAS-01.

NCBI tax ID(s): 1441386 (species)

Section

Athalassotoga saccharophila NAS-01 is an anaerobe, Gram-negative, rod-shaped bacterium that was isolated from low-salt acidic terrestrial hot spring.

Gram-negative
rod-shaped
anaerobe
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	07-12-2022 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Thermotogota*
	Class	*Thermotogae*
	Order	*Mesoaciditogales*
	Family	*Mesoaciditogaceae*
	Genus	*Athalassotoga*
	Species	**Athalassotoga saccharophila**
	Full Scientific Name (PNU)	Athalassotoga saccharophila Itoh et al. 2016

Information on morphological and physiological properties Morphology

[Ref.: #44023]	Gram stain	negative
[Ref.: #44023]	Cell length	0.8-2.0 µm
[Ref.: #44023]	Cell width	0.7-0.8 µm
[Ref.: #44023]	Cell shape	rod-shaped
[Ref.: #44023]	Motility	no

[Ref.: #69480]	Gram stain	negative Confidence in %99.886

Information on culture and growth conditions Culture and growth conditions

[Ref.: #24205]

Culture medium

ATHALASSOTOGA MEDIUM (DSMZ Medium 1604)

[Ref.: #24205]

Culture medium growth

[Ref.: #24205]

Culture medium link

Medium recipe provided by DSMZ

[Ref.: #24205]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #24205]	growth	55 °C
[Ref.: #44023]	growth	30-60 °C
[Ref.: #44023]	optimum	55 °C
[Ref.: #67770]	growth	55 °C

[Ref.: #24205]	Temperature range	thermophilic
[Ref.: #44023]	Temperature range	thermophilic
[Ref.: #67770]	Temperature range	thermophilic
[Ref.: #69480]	Temperature range	thermophilic Confidence in %99.746

	pH	Kind of pH		pH
[Ref.: #44023]			optimum	5.5-6.0
[Ref.: #44023]			growth	4.5-7.5

Information on physiology and metabolism Physiology and metabolism

[Ref.: #44023]	Oxygen tolerance	anaerobe
[Ref.: #69480]	Oxygen tolerance	anaerobe Confidence in %100

[Ref.: #44023]	Ability of spore formation	no
[Ref.: #69480]	Ability of spore formation	no Confidence in %99.986

[Ref.: #44023]

Halophily / tolerance level

non-halophilic

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #44023]		NaCl		growth	0-1	%
[Ref.: #44023]		NaCl		optimum	0	%

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #44023]	acetate ChEBI	-	carbon source
[Ref.: #44023]	acetate ChEBI	-	energy source
[Ref.: #44023]	butyrate ChEBI	-	carbon source
[Ref.: #44023]	butyrate ChEBI	-	energy source
[Ref.: #44023]	casamino acids	-	carbon source
[Ref.: #44023]	casamino acids	-	energy source
[Ref.: #44023]	citrate ChEBI	-	carbon source
[Ref.: #44023]	citrate ChEBI	-	energy source
[Ref.: #44023]	D-fructose ChEBI	+	carbon source
[Ref.: #44023]	D-fructose ChEBI	+	energy source
[Ref.: #44023]	D-glucose ChEBI	+	carbon source
[Ref.: #44023]	D-glucose ChEBI	+	energy source
[Ref.: #44023]	D-mannose ChEBI	+	carbon source
[Ref.: #44023]	D-mannose ChEBI	+	energy source
[Ref.: #44023]	D-xylose ChEBI	+	carbon source
[Ref.: #44023]	D-xylose ChEBI	+	energy source
[Ref.: #44023]	ferric iron ChEBI	+	electron acceptor
[Ref.: #44023]	formate ChEBI	-	carbon source
[Ref.: #44023]	formate ChEBI	-	energy source
[Ref.: #44023]	fumarate ChEBI	-	carbon source
[Ref.: #44023]	fumarate ChEBI	-	energy source
[Ref.: #44023]	galactose ChEBI	-	carbon source
[Ref.: #44023]	galactose ChEBI	-	energy source
[Ref.: #44023]	glycogen ChEBI	+	carbon source
[Ref.: #44023]	glycogen ChEBI	+	energy source
[Ref.: #44023]	L-arabinose ChEBI	+	carbon source
[Ref.: #44023]	L-arabinose ChEBI	+	energy source
[Ref.: #44023]	L-cystine ChEBI	+	electron acceptor
[Ref.: #44023]	lactate ChEBI	-	carbon source
[Ref.: #44023]	lactate ChEBI	-	energy source
[Ref.: #44023]	lactose ChEBI	+	carbon source
[Ref.: #44023]	lactose ChEBI	+	energy source
[Ref.: #44023]	malate ChEBI	-	carbon source
[Ref.: #44023]	malate ChEBI	-	energy source
[Ref.: #44023]	maltose ChEBI	+	carbon source
[Ref.: #44023]	maltose ChEBI	+	energy source
[Ref.: #44023]	peptone	+	carbon source
[Ref.: #44023]	peptone	+	energy source
[Ref.: #44023]	propionate ChEBI	-	carbon source
[Ref.: #44023]	propionate ChEBI	-	energy source
[Ref.: #44023]	pyruvate ChEBI	-	carbon source
[Ref.: #44023]	pyruvate ChEBI	-	energy source
[Ref.: #44023]	ribose ChEBI	+	carbon source
[Ref.: #44023]	ribose ChEBI	+	energy source
[Ref.: #44023]	starch ChEBI	+	carbon source
[Ref.: #44023]	starch ChEBI	+	energy source
[Ref.: #44023]	succinate ChEBI	-	carbon source
[Ref.: #44023]	succinate ChEBI	-	energy source
[Ref.: #44023]	sucrose ChEBI	+	carbon source
[Ref.: #44023]	sucrose ChEBI	+	energy source
[Ref.: #44023]	thiosulfate ChEBI	+	electron acceptor
[Ref.: #44023]	yeast extract	+	carbon source
[Ref.: #44023]	yeast extract	+	energy source
	Only first 10 entries are displayed. Click here to see all.

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #24205]	Sample type/isolated from	low-salt acidic terrestrial hot spring
[Ref.: #24205]	Geographic location (country and/or sea, region)	Tochigi prefecture, Okushiobara
[Ref.: #24205]	Country	Japan
[Ref.: #24205]	Country ISO 3 Code	JPN
[Ref.: #24205]	Continent	Asia
[Ref.: #24205]	Geographic location	36.9547°/139.7800°

[Ref.: #44023]	Sample type/isolated from	acidic hot spring water
[Ref.: #44023]	Sampling date	2012-04
[Ref.: #44023]	Geographic location (country and/or sea, region)	Oku-Shiobara, Tochigi
[Ref.: #44023]	Country	Japan
[Ref.: #44023]	Country ISO 3 Code	JPN
[Ref.: #44023]	Continent	Asia
[Ref.: #44023]	Geographic location	36.9547°/139.7800°
[Ref.: #44023]	Enrichment culture composition	0.2g (NH 4)2 SO4, 3.0?g KH2PO4, 0.5?g MgSO 4?.?7H2O, 0.25?g CaCl2?.?2H2O, 1.8?mg MnCl2?.?4H2O, 4.5?mg Na2B4O7?.?10H2O, 0.22?mg ZnSO4?.7H2O, 0.05?mg CuCl2?.?2H2O, 0.03?mg Na2MoO4?.?2H2O, 0.03?mg VOSO4?.?nH2O, 0.01?mg CoSO4?.?7H2O, 0.5?g yeast extract (BD-Difco), 10?mM Fe(III)citrate and 10.0?ml vitamin mixture of Balch et al. (1979); pH adjusted to 5.0
[Ref.: #44023]	Enrichment culture duration	2 weeks
[Ref.: #44023]	Enrichment culture temperature	55 ̊C
[Ref.: #44023]	Isolation/enrichment procedure	The medium was placed in culture bottles under a gas phase of N2/CO2 (4 : 1, v/v), and reduced with 0.25 mM L-cysteine.HCl and 1.3 mM FeCl2 . 4H2O

[Ref.: #67770]	Sample type/isolated from	Low-salt acidic terrestrial hot spring in Oku-Shiobara
[Ref.: #67770]	Geographic location (country and/or sea, region)	Tochigi
[Ref.: #67770]	Country	Japan
[Ref.: #67770]	Country ISO 3 Code	JPN
[Ref.: #67770]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Environmental	#Terrestrial	-
#Condition	#Thermophilic (>45°C)	-
#Condition	#Acidic	-
#Environmental	#Aquatic	#Thermal spring

What are isolation sources categories?

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #24205]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #24205]	Thermotogales bacterium NAS-01 gene for 16S ribosomal RNA, partial sequence	AB898788	1500	ENA	1441386 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Athalassotoga saccharophila NAS-01 DNA, complete genome	AP019551		GenBank	1441386 tax ID
[Ref.: #66792]	Athalassotoga saccharophila NAS-01	GCA_009936215	complete	GenBank	1441386 tax ID	*
[Ref.: #66792]	Athalassotoga saccharophila strain NAS-01	1441386.9	wgs	PATRIC	1441386 tax ID	*
[Ref.: #66792]	Athalassotoga saccharophila NAS-01	2883451585	complete	JGI IMG/M	1441386 tax ID	*

[Ref.: #24205]

GC-content

41.1 mol%

high performance liquid chromatography (HPLC)

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Athalassotoga saccharophila NAS-01 NCBI: GCA_009936215.1
[Ref.: #69480]	gram-positive	no	99.886	no
[Ref.: #69480]	anaerobic	yes	100	yes
[Ref.: #69480]	spore-forming	no	99.986	yes
[Ref.: #69480]	thermophile	yes	99.746	yes
[Ref.: #69480]	motile	no	73.491	yes

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Athalassotoga saccharophila NAS-01 NCBI: GCA_009936215.1
[Ref.: #69481]	spore-forming	no	80	no

Availability in culture collections External links

[Ref.: #24205]

Culture collection no.

DSM 28954, JCM 19762

Literature:

Topic	Title	Authors	Journal	DOI	Year
Genetics	Complete Genome Sequence of Athalassotoga saccharophila Strain NAS-01, a Deep-Branching Thermophilic Lineage in the Phylum Thermotogae.	Kato S, Itoh T, Ohkuma M	Microbiol Resour Announc	10.1128/MRA.00322-20	2020	*
Phylogeny	Athalassotoga saccharophila gen. nov., sp. nov., isolated from an acidic terrestrial hot spring, and proposal of Mesoaciditogales ord. nov. and Mesoaciditogaceae fam. nov. in the phylum Thermotogae.	Itoh T, Onishi M, Kato S, Iino T, Sakamoto M, Kudo T, Takashina T, Ohkuma M	Int J Syst Evol Microbiol	10.1099/ijsem.0.000833	2015	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#24205

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 28954

#44023

Takashi Itoh, Masafumi Onishi, Shingo Kato, Takao Iino, Mitsuo Sakamoto, Takuji Kudo, Tomonori Takashina and Moriya Ohkuma: Athalassotoga saccharophila gen. nov., sp. nov., isolated from an acidic terrestrial hot spring, and proposal of Mesoaciditogales ord. nov. and Mesoaciditogaceae fam. nov. in the phylum Thermotogae. IJSEM 66: 1045 - 1051 2016 ( DOI 10.1099/ijsem.0.000833 , PubMed 26651491 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

* These data were automatically processed and therefore are not curated

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