Strain identifier

BacDive ID: 132319

Type strain: No

Species: Methylibium sp.

Strain Designation: Root1272

Strain history: <- P. Schulze-Lefert, Max Planck Institute for Plant Breeding Research, Cologne, Germany; Root1272 <- Y. Bai

NCBI tax ID(s): 2067992 (species)

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General

@ref: 24108

BacDive-ID: 132319

DSM-Number: 102455

keywords: genome sequence, Bacteria, Gram-negative

description: Methylibium sp. Root1272 is a Gram-negative bacterium that was isolated from roots of Arabidopsis thaliana ecotype Shakdara .

NCBI tax id

  • NCBI tax id: 2067992
  • Matching level: species

strain history

  • @ref: 24108
  • history: <- P. Schulze-Lefert, Max Planck Institute for Plant Breeding Research, Cologne, Germany; Root1272 <- Y. Bai

doi: 10.13145/bacdive132319.20240916.9.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Betaproteobacteria
  • order: Burkholderiales
  • family: Burkholderiales, not assigned to family
  • genus: Methylibium
  • species: Methylibium sp.
  • full scientific name: Methylibium Nakatsu et al. 2006
  • synonyms

    • @ref: 20215
    • synonym: Pseudorivibacter

@ref: 24108

domain: Bacteria

phylum: Proteobacteria

class: Betaproteobacteria

order: Betaproteobacteria, not assigned to order

family: Burkholderiales, not assigned to family

genus: Methylibium

species: Methylibium sp.

full scientific name: Methylibium sp.

strain designation: Root1272

type strain: no

Morphology

cell morphology

  • @ref: 69480
  • gram stain: negative
  • confidence: 94.894

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
24108R2A MEDIUM (DSMZ Medium 830)yeshttps://mediadive.dsmz.de/medium/830Name: R2A MEDIUM (DSMZ Medium 830) Composition: Agar 15.0 g/l Casamino acids 0.5 g/l Starch 0.5 g/l Glucose 0.5 g/l Proteose peptone 0.5 g/l Yeast extract 0.5 g/l K2HPO4 0.3 g/l Na-pyruvate 0.3 g/l MgSO4 x 7 H2O 0.05 g/l Distilled water
24108TRYPTONE YEAST EXTRACT GLUCOSE MEDIUM (TYG) (DSMZ Medium 1618)yeshttps://mediadive.dsmz.de/medium/1618Name: TRYPTONE YEAST EXTRACT GLUCOSE MEDIUM (TYG) (DSMZ Medium 1618) Composition: Agar 20.0 g/l KCl 6.34 g/l Na2SO4 2.4 g/l NaCl 1.2 g/l Tryptone 1.0 g/l Yeast extract 1.0 g/l NaHCO3 0.5 g/l D-Glucose 0.5 g/l MgSO4 x 7 H2O 0.25 g/l CaCl2 x 2 H2O 0.22 g/l K2SO4 0.17 g/l K2HPO4 0.13 g/l Na2CO3 0.09 g/l Fe(III)-EDTA 0.07 g/l Distilled water

culture temp

  • @ref: 24108
  • growth: positive
  • type: growth
  • temperature: 28

Physiology and metabolism

spore formation

  • @ref: 69480
  • spore formation: no
  • confidence: 93.835

Isolation, sampling and environmental information

isolation

  • @ref: 24108
  • sample type: roots of Arabidopsis thaliana ecotype Shakdara (Sha)
  • host species: Arabidopsis thaliana
  • geographic location: Cologne (50.958N 6.856E)
  • country: Germany
  • origin.country: DEU
  • continent: Europe
  • latitude: 50.958
  • longitude: 6.856

isolation source categories

Cat1Cat2Cat3
#Host#Plants#Herbaceous plants (Grass,Crops)
#Host Body-Site#Plant#Root (Rhizome)

Sequence information

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Methylibium sp. Root1272GCA_001425905contigncbi1736441
66792Methylibium sp. Root12721736441.3wgspatric1736441
66792Methylibium sp. Root12722643221660draftimg1736441

Genome-based predictions

predictions

@reftraitmodeldescriptionpredictionconfidencetraining_data
69480gram-positivegram-positivePositive reaction to Gram-stainingno94.894no
69480anaerobicanaerobicAbility to grow under anoxygenic conditions (including facultative anaerobes)no97.887no
69480spore-formingspore-formingAbility to form endo- or exosporesno93.835no
69480aerobicaerobicAbility to grow under oxygenic conditions (including facultative aerobes)yes79.863no
69480thermophilicthermophileAbility to grow at temperatures above or equal to 45°Cno97.857yes
69480flagellatedmotile2+Ability to perform flagellated movementyes81.115no

External links

@ref: 24108

culture collection no.: DSM 102455

straininfo link

  • @ref: 90666
  • straininfo: 406674

Reference

@idauthorstitledoi/urlcatalogue
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
24108Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-102455Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 102455)
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
90666Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID406674.1