Strain identifier
BacDive ID: 132208
Type strain: 
Species: Anaerosolibacter carboniphilus
Strain Designation: IRF19
Strain history: <- Sung-Keun Rhee, Chungbuk National Univ.
NCBI tax ID(s): 1417629 (species)
version 8.1 (current version)
General
@ref: 23997
BacDive-ID: 132208
DSM-Number: 103526
keywords: genome sequence, 16S sequence, Bacteria, anaerobe, spore-forming, thermophilic, motile, rod-shaped
description: Anaerosolibacter carboniphilus IRF19 is an anaerobe, spore-forming, thermophilic bacterium that was isolated from contaminated soil from a coal mine tail.
NCBI tax id
- NCBI tax id: 1417629
- Matching level: species
strain history
| @ref | history |
|---|---|
| 23997 | <- KCTC <- S.-K. Rhee, Chungbuk Natl. Univ., Korea |
| 67770 | S.-K. Rhee; Chungbuk Natl. Univ., South Korea; IRF19. |
| 67771 | <- Sung-Keun Rhee, Chungbuk National Univ. |
doi: 10.13145/bacdive132208.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/bacillota
- domain: Bacteria
- phylum: Bacillota
- class: Clostridia
- order: Eubacteriales
- family: Clostridiaceae
- genus: Anaerosolibacter
- species: Anaerosolibacter carboniphilus
- full scientific name: Anaerosolibacter carboniphilus Hong et al. 2015
@ref: 23997
domain: Bacteria
phylum: Firmicutes
class: Clostridia
order: Clostridiales
family: Clostridiaceae
genus: Anaerosolibacter
species: Anaerosolibacter carboniphilus
full scientific name: Anaerosolibacter carboniphilus Hong et al. 2015
strain designation: IRF19
type strain: yes
Morphology
cell morphology
| @ref | cell shape | motility | gram stain | confidence |
|---|---|---|---|---|
| 67771 | rod-shaped | yes | ||
| 67771 | negative | |||
| 69480 | yes | 95.306 | ||
| 69480 | positive | 100 |
Culture and growth conditions
culture medium
| @ref | name | growth | link | composition |
|---|---|---|---|---|
| 23997 | ANAEROBIC FRESHWATER (FWM) MEDIUM (DSMZ Medium 503) | yes | https://mediadive.dsmz.de/medium/503 | Name: ANAEROBIC FRESHWATER (FWM) MEDIUM (DSMZ Medium 503) Composition: D-Glucose 4.98504 g/l Na2CO3 1.49551 g/l NaCl 0.997008 g/l KCl 0.498505 g/l MgCl2 x 6 H2O 0.398803 g/l Na2S x 9 H2O 0.299103 g/l NH4Cl 0.249252 g/l KH2PO4 0.199402 g/l CaCl2 x 2 H2O 0.149552 g/l HCl 0.00249252 g/l FeCl2 x 4 H2O 0.00149551 g/l Sodium resazurin 0.000498505 g/l NaOH 0.000498504 g/l Pyridoxine hydrochloride 0.000299103 g/l Nicotinic acid 0.000199402 g/l Thiamine-HCl x 2 H2O 0.000199402 g/l CoCl2 x 6 H2O 0.000189432 g/l Calcium pantothenate 9.97009e-05 g/l MnCl2 x 4 H2O 9.97009e-05 g/l Vitamin B12 9.97009e-05 g/l p-Aminobenzoic acid 7.97607e-05 g/l ZnCl2 6.97906e-05 g/l Na2MoO4 x 2 H2O 3.58923e-05 g/l NiCl2 x 6 H2O 2.39282e-05 g/l D-(+)-biotin 1.99402e-05 g/l H3BO3 5.98205e-06 g/l Na2WO4 x 2 H2O 3.98804e-06 g/l Na2SeO3 x 5 H2O 2.99103e-06 g/l CuCl2 x 2 H2O 1.99402e-06 g/l Distilled water |
| 23997 | PY + X MEDIUM (DSMZ Medium 104b) | yes | https://mediadive.dsmz.de/medium/104b | Name: PY + X MEDIUM (DSMZ Medium 104b) Composition: Yeast extract 10.0 g/l D-Glucose 5.0 g/l Trypticase peptone 5.0 g/l Meat peptone 5.0 g/l L-Cysteine HCl x H2O 0.5 g/l NaHCO3 0.4 g/l NaCl 0.08 g/l KH2PO4 0.04 g/l K2HPO4 0.04 g/l MgSO4 x 7 H2O 0.02 g/l CaCl2 x 2 H2O 0.01 g/l Sodium resazurin 0.0005 g/l Distilled water |
culture temp
| @ref | growth | type | temperature | range |
|---|---|---|---|---|
| 23997 | positive | growth | 40 | thermophilic |
| 67770 | positive | growth | 40 | thermophilic |
| 67771 | positive | growth | 40 | thermophilic |
Physiology and metabolism
oxygen tolerance
| @ref | oxygen tolerance | confidence |
|---|---|---|
| 67771 | anaerobe | |
| 69480 | anaerobe | 98.649 |
spore formation
| @ref | spore formation | confidence |
|---|---|---|
| 69481 | yes | 93 |
| 69480 | yes | 100 |
observation
| @ref | observation |
|---|---|
| 67770 | quinones: MK-5(V-H2) |
| 67771 | quinones: MK-5 (V-H2) |
Isolation, sampling and environmental information
isolation
| @ref | sample type | country | origin.country | continent | latitude | longitude |
|---|---|---|---|---|---|---|
| 23997 | contaminated soil from a coal mine tail | Republic of Korea | KOR | Asia | ||
| 67770 | Contaminated soil from a coal mine tail | Republic of Korea | KOR | Asia | ||
| 67771 | From petroleum contaminated soil, coal mine tail | Republic of Korea | KOR | Asia | 37.1667 | 128.967 |
isolation source categories
| Cat1 | Cat2 | Cat3 |
|---|---|---|
| #Engineered | #Contamination | |
| #Engineered | #Other | #Mine |
| #Environmental | #Terrestrial | #Soil |
taxonmaps
- @ref: 69479
- File name: preview.99_63913.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_15791;96_1129;97_35026;98_45215;99_63913&stattab=map
- Last taxonomy: Anaerosolibacter carboniphilus subclade
- 16S sequence: KF601947
- Sequence Identity:
- Total samples: 3352
- soil counts: 1524
- aquatic counts: 746
- animal counts: 828
- plant counts: 254
Safety information
risk assessment
- @ref: 23997
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 23997
- description: Anaerosolibacter carboniphilus strain IRF19 16S ribosomal RNA gene, partial sequence
- accession: KF601947
- length: 1412
- database: ena
- NCBI tax ID: 1417629
Genome sequences
| @ref | description | accession | assembly level | database | NCBI tax ID |
|---|---|---|---|---|---|
| 66792 | Anaerosolibacter carboniphilus DSM 103526 | GCA_014205875 | scaffold | ncbi | 1417629 |
| 66792 | Anaerosolibacter carboniphilus strain DSM 103526 | 1417629.3 | wgs | patric | 1417629 |
| 66792 | Anaerosolibacter carboniphilus DSM 103526 | 2861187593 | draft | img | 1417629 |
GC content
| @ref | GC-content | method |
|---|---|---|
| 23997 | 37.4 | |
| 67770 | 37.4 | thermal denaturation, midpoint method (Tm) |
Genome-based predictions
predictions
| trait | prediction | confidence | training_data |
|---|---|---|---|
| spore-forming | yes | 93 | no |
| flagellated | yes | 63.983 | no |
| gram-positive | no | 74.137 | yes |
| anaerobic | yes | 96.934 | yes |
| aerobic | no | 97.178 | yes |
| halophile | no | 91.181 | no |
| spore-forming | yes | 93.416 | no |
| glucose-util | yes | 91.096 | no |
| thermophile | no | 88.72 | no |
| motile | yes | 82.549 | no |
| glucose-ferment | no | 62.711 | no |
External links
@ref: 23997
culture collection no.: DSM 103526, JCM 19988, KCTC 15396
straininfo link
- @ref: 90561
- straininfo: 403109
literature
- topic: Phylogeny
- Pubmed-ID: 25701849
- title: Anaerosolibacter carboniphilus gen. nov., sp. nov., a strictly anaerobic iron-reducing bacterium isolated from coal-contaminated soil.
- authors: Hong H, Kim SJ, Min UG, Lee YJ, Kim SG, Roh SW, Kim JG, Na JG, Rhee SK
- journal: Int J Syst Evol Microbiol
- DOI: 10.1099/ijs.0.000124
- year: 2015
- mesh: Bacterial Typing Techniques, Base Composition, Coal Mining, DNA, Bacterial/genetics, Environmental Pollution, Fatty Acids/chemistry, Gram-Negative Anaerobic Straight, Curved, and Helical Rods/*classification/genetics/isolation & purification, Iron/metabolism, Molecular Sequence Data, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Republic of Korea, Sequence Analysis, DNA, *Soil Microbiology, Vitamin K 2/chemistry
- topic2: Genetics
Reference
| @id | authors | title | doi/url | catalogue |
|---|---|---|---|---|
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | 10.1099/ijsem.0.004332 | |
| 23997 | Curators of the DSMZ | https://www.dsmz.de/collection/catalogue/details/culture/DSM-103526 | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 103526) | |
| 66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | https://diaspora-project.de/progress.html#genomes | |
| 67770 | Curators of the JCM | https://jcm.brc.riken.jp/en/ | ||
| 67771 | Curators of the KCTC | https://kctc.kribb.re.kr/En/Kctc | ||
| 69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | MicrobeAtlas 1.0 beta | https://microbeatlas.org/ | |
| 69480 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | https://diaspora-project.de/progress.html#genomes | |
| 69481 | Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch | Predictions based on the model GenomeNet Sporulation v. 1 | 10.21203/rs.3.rs-2527258/v1 | |
| 90561 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | StrainInfo: A central database for resolving microbial strain identifiers | 10.60712/SI-ID403109.1 |