Strain identifier
BacDive ID: 132056
Type strain: 
Species: Nitrosospira multiformis
Strain Designation: C-71, Nl13
Strain history: <- A. Pommerening-Röser, Universität Hamburg, Department of Microbiology and Biotechnology, Germany; Nl13 <- S. W. Watson, Woods Hole Oceanographic Institute, Massachusetts, USA; C-71
NCBI tax ID(s): 323848 (strain), 1231 (species)
version 8.1 (current version)
General
@ref: 23847
BacDive-ID: 132056
DSM-Number: 101674
keywords: genome sequence, 16S sequence, Bacteria, mesophilic, Gram-negative
description: Nitrosospira multiformis C-71 is a mesophilic, Gram-negative bacterium that was isolated from soil.
NCBI tax id
| NCBI tax id | Matching level |
|---|---|
| 1231 | species |
| 323848 | strain |
strain history
- @ref: 23847
- history: <- A. Pommerening-Röser, Universität Hamburg, Department of Microbiology and Biotechnology, Germany; Nl13 <- S. W. Watson, Woods Hole Oceanographic Institute, Massachusetts, USA; C-71
doi: 10.13145/bacdive132056.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/pseudomonadota
- domain: Bacteria
- phylum: Pseudomonadota
- class: Betaproteobacteria
- order: Nitrosomonadales
- family: Nitrosomonadaceae
- genus: Nitrosospira
- species: Nitrosospira multiformis
- full scientific name: Nitrosospira multiformis (Watson et al. 1971) Head et al. 1995
synonyms
- @ref: 20215
- synonym: Nitrosolobus multiformis
@ref: 23847
domain: Bacteria
phylum: Proteobacteria
class: Betaproteobacteria
order: Nitrosomonadales
family: Nitrosomonadaceae
genus: Nitrosospira
species: Nitrosospira multiformis
full scientific name: Nitrosospira multiformis (Watson et al. 1971) Head et al. 1995
strain designation: C-71, Nl13
type strain: yes
Morphology
cell morphology
| @ref | gram stain | confidence | motility |
|---|---|---|---|
| 69480 | negative | 99.999 | |
| 69480 | 93.995 | yes |
Culture and growth conditions
culture medium
- @ref: 23847
- name: MEDIUM FOR AMMONIA OXIDIZING BACTERIA (STRAINS FROM SOIL) (DSMZ Medium 1583)
- growth: yes
- link: https://mediadive.dsmz.de/medium/1583
- composition: Name: MEDIUM FOR AMMONIA OXIDIZING BACTERIA (STRAINS FROM SOIL) (DSMZ Medium 1583) Composition: NaCl 0.584 g/l NH4Cl 0.535 g/l CaCl2 x 2 H2O 0.147 g/l KCl 0.074 g/l KH2PO4 0.054 g/l MgSO4 x 7 H2O 0.049 g/l Cresol red 0.001 g/l FeSO4 x 7 H2O 0.000973 g/l H3BO3 4.9e-05 g/l MnSO4 x 4 H2O 4.5e-05 g/l ZnSO4 x 7 H2O 4.3e-05 g/l (NH4)6Mo7O24 x 4 H2O 3.7e-05 g/l CuSO4 x 5 H2O 2.5e-05 g/l HCl Distilled water
culture temp
- @ref: 23847
- growth: positive
- type: growth
- temperature: 28
- range: mesophilic
Physiology and metabolism
spore formation
- @ref: 69480
- spore formation: no
- confidence: 99.998
Isolation, sampling and environmental information
isolation
- @ref: 23847
- sample type: soil
- geographic location: Paramaribo
- country: Suriname
- origin.country: SUR
- continent: Middle and South America
isolation source categories
- Cat1: #Environmental
- Cat2: #Terrestrial
- Cat3: #Soil
taxonmaps
- @ref: 69479
- File name: preview.99_2755.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_316;96_1467;97_1749;98_2138;99_2755&stattab=map
- Last taxonomy: Nitrosospira multiformis
- 16S sequence: AY123807
- Sequence Identity:
- Total samples: 2084
- soil counts: 1557
- aquatic counts: 320
- animal counts: 88
- plant counts: 119
Safety information
risk assessment
- @ref: 23847
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 23847
- description: Nitrosospira multiformis 16S ribosomal RNA gene, partial sequence
- accession: AY123807
- length: 1498
- database: ena
- NCBI tax ID: 323848
Genome sequences
| @ref | description | accession | assembly level | database | NCBI tax ID |
|---|---|---|---|---|---|
| 66792 | Nitrosospira multiformis ATCC 25196 | GCA_000196355 | complete | ncbi | 323848 |
| 66792 | Nitrosospira multiformis ATCC 25196 Nl13 | GCA_900108135 | contig | ncbi | 323848 |
| 66792 | Nitrosospira multiformis ATCC 25196 | 323848.14 | plasmid | patric | 323848 |
| 66792 | Nitrosospira multiformis ATCC 25196 | 323848.15 | plasmid | patric | 323848 |
| 66792 | Nitrosospira multiformis ATCC 25196 | 323848.4 | complete | patric | 323848 |
| 66792 | Nitrosospira multiformis ATCC 25196 | 323848.16 | plasmid | patric | 323848 |
| 66792 | Nitrosospira multiformis ATCC 25196 strain Nl13 | 323848.18 | wgs | patric | 323848 |
| 66792 | Nitrosospira multiformis ATCC 25196 | 637000197 | complete | img | 323848 |
| 66792 | Nitrosospira multiformis Nl13 | 2671180127 | draft | img | 1231 |
Genome-based predictions
predictions
| trait | prediction | confidence | training_data |
|---|---|---|---|
| spore-forming | yes | 50 | no |
| motile | yes | 84.959 | no |
| flagellated | no | 62.01 | no |
| gram-positive | no | 98.418 | no |
| anaerobic | no | 97.368 | no |
| aerobic | no | 61.589 | no |
| halophile | no | 91.773 | no |
| spore-forming | no | 91.201 | no |
| thermophile | no | 95.578 | no |
| glucose-util | no | 61.64 | no |
| glucose-ferment | no | 88.712 | no |
External links
@ref: 23847
culture collection no.: DSM 101674, ATCC 25196
straininfo link
- @ref: 90414
- straininfo: 38407
literature
| topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
|---|---|---|---|---|---|---|---|---|
| Phylogeny | 7961414 | Evolutionary relationships among ammonia- and nitrite-oxidizing bacteria. | Teske A, Alm E, Regan JM, Toze S, Rittmann BE, Stahl DA | J Bacteriol | 10.1128/jb.176.21.6623-6630.1994 | 1994 | Ammonia/metabolism, Base Sequence, Biological Evolution, Bradyrhizobiaceae/*classification/*genetics, Molecular Sequence Data, Nitrites/metabolism, Nitrobacter/classification/genetics, Nitrosomonas/classification/genetics, Oxidation-Reduction, RNA, Ribosomal, 16S/*genetics, Sequence Analysis, RNA, Sequence Homology, Nucleic Acid | Metabolism |
| Phylogeny | 8566698 | Comparative analysis of gene sequences encoding ammonia monooxygenase of Nitrosospira sp. AHB1 and Nitrosolobus multiformis C-71. | Rotthauwe JH, de Boer W, Liesack W | FEMS Microbiol Lett | 10.1111/j.1574-6968.1995.tb07873.x | 1995 | Amino Acid Sequence, Base Sequence, Bradyrhizobiaceae/enzymology/*genetics, DNA, Bacterial/analysis, DNA, Ribosomal/analysis, Molecular Sequence Data, Oxidoreductases/*genetics, Phylogeny, *Soil Microbiology | Enzymology |
| Metabolism | 12710742 | Susceptibility of ammonia-oxidizing bacteria to nitrification inhibitors. | Matsuba D, Takazaki H, Sato Y, Takahashi R, Tokuyama T, Wakabayashi K | Z Naturforsch C J Biosci | 10.1515/znc-2003-3-424 | 2003 | Ammonia/*pharmacokinetics, Bradyrhizobiaceae/classification/*drug effects/*metabolism, DNA, Ribosomal/genetics, Environment, Nitric Oxide/*metabolism, Nitrites/*metabolism, Nitrobacter/drug effects/metabolism, Nitrosomonas/drug effects/metabolism, Oxidation-Reduction, Phylogeny, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Triazines/*pharmacology | Phylogeny |
| Phylogeny | 16233089 | Characteristics of an ammonia-oxidizing bacterium with a plasmid isolated from alkaline soils and its phylogenetic relationship. | Takahashi R, Ohishi M, Ohshima M, Saitoh M, Omata K, Tokuyama T | J Biosci Bioeng | 10.1263/jbb.92.232 | 2001 | ||
| Genetics | 18390676 | Complete genome sequence of Nitrosospira multiformis, an ammonia-oxidizing bacterium from the soil environment. | Norton JM, Klotz MG, Stein LY, Arp DJ, Bottomley PJ, Chain PS, Hauser LJ, Land ML, Larimer FW, Shin MW, Starkenburg SR | Appl Environ Microbiol | 10.1128/AEM.02722-07 | 2008 | Ammonia/*metabolism, Bacterial Proteins/genetics, Chromosomes, Bacterial, DNA, Bacterial/*chemistry/genetics, Energy Metabolism/genetics, Gene Dosage, *Genome, Bacterial, Metabolism/genetics, Molecular Sequence Data, Multigene Family, Nitrosomonadaceae/*genetics/isolation & purification, Open Reading Frames, Plasmids, Repetitive Sequences, Nucleic Acid, Sequence Analysis, DNA, Sequence Homology, Amino Acid, *Soil Microbiology | Metabolism |
| Pathogenicity | 20630557 | Various physico-chemical stress factors cause prophage induction in Nitrosospira multiformis 25196--an ammonia oxidizing bacteria. | Choi J, Kotay SM, Goel R | Water Res | 10.1016/j.watres.2010.04.040 | 2010 | Ammonia/metabolism, Antibiotics, Antineoplastic/toxicity, Bacteriolysis/drug effects, Chromium/chemistry/toxicity, Hydrogen-Ion Concentration, Microscopy, Electron, Microscopy, Fluorescence, Mitomycin/toxicity, Nitrosomonadaceae/metabolism/*virology, Organic Chemicals/chemistry/toxicity, Oxidation-Reduction/drug effects, Potassium Cyanide/chemistry/toxicity, Prophages/*physiology, Temperature, Virion/*physiology/ultrastructure, Virus Activation/drug effects, Water Pollutants, Chemical/*toxicity | Metabolism |
| Metabolism | 21470297 | Effects of nitrite on ammonia-oxidizing activity and gene regulation in three ammonia-oxidizing bacteria. | Cua LS, Stein LY | FEMS Microbiol Lett | 10.1111/j.1574-6968.2011.02277.x | 2011 | Ammonia/*metabolism, Bacterial Proteins/genetics/metabolism, *Gene Expression Regulation, Bacterial, Molecular Sequence Data, Nitrites/*metabolism, Nitrosomonas/genetics/*metabolism, Oxidation-Reduction | Genetics |
| Phylogeny | 25336720 | Nitrosospira lacus sp. nov., a psychrotolerant, ammonia-oxidizing bacterium from sandy lake sediment. | Urakawa H, Garcia JC, Nielsen JL, Le VQ, Kozlowski JA, Stein LY, Lim CK, Pommerening-Roser A, Martens-Habbena W, Stahl DA, Klotz MG | Int J Syst Evol Microbiol | 10.1099/ijs.0.070789-0 | 2014 | Ammonia/*metabolism, Base Composition, DNA, Bacterial/genetics, Lakes/*microbiology, Molecular Sequence Data, Nitrosomonadaceae/*classification/genetics/isolation & purification, Nucleic Acid Hybridization, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA | Genetics |
| Metabolism | 26882267 | Pathways and key intermediates required for obligate aerobic ammonia-dependent chemolithotrophy in bacteria and Thaumarchaeota. | Kozlowski JA, Stieglmeier M, Schleper C, Klotz MG, Stein LY | ISME J | 10.1038/ismej.2016.2 | 2016 | Ammonia/*metabolism, Archaea/*metabolism, Bacteria/*metabolism, Chemoautotrophic Growth, Hydroxylamine/metabolism, Metabolic Networks and Pathways, Nitrites/metabolism, Nitrous Oxide/*metabolism, Oxidation-Reduction | |
| 27471578 | Complete genome of Nitrosospira briensis C-128, an ammonia-oxidizing bacterium from agricultural soil. | Rice MC, Norton JM, Valois F, Bollmann A, Bottomley PJ, Klotz MG, Laanbroek HJ, Suwa Y, Stein LY, Sayavedra-Soto L, Woyke T, Shapiro N, Goodwin LA, Huntemann M, Clum A, Pillay M, Kyrpides N, Varghese N, Mikhailova N, Markowitz V, Palaniappan K, Ivanova N, Stamatis D, Reddy TB, Ngan CY, Daum C | Stand Genomic Sci | 10.1186/s40793-016-0168-4 | 2016 | |||
| Phylogeny | 33433313 | Usitatibacter rugosus gen. nov., sp. nov. and Usitatibacter palustris sp. nov., novel members of Usitatibacteraceae fam. nov. within the order Nitrosomonadales isolated from soil. | Vieira S, Huber KJ, Neumann-Schaal M, Geppert A, Luckner M, Wanner G, Overmann J | Int J Syst Evol Microbiol | 10.1099/ijsem.0.004631 | 2021 | Bacterial Typing Techniques, Base Composition, Betaproteobacteria/*classification/isolation & purification, DNA, Bacterial/genetics, Fatty Acids/chemistry, Germany, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Soil Microbiology, Ubiquinone/chemistry | Transcriptome |
Reference
| @id | authors | title | doi/url | catalogue | journal | pubmed |
|---|---|---|---|---|---|---|
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | 10.1099/ijsem.0.004332 | |||
| 23847 | Curators of the DSMZ | https://www.dsmz.de/collection/catalogue/details/culture/DSM-101674 | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 101674) | |||
| 66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | https://diaspora-project.de/progress.html#genomes | |||
| 66794 | Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg | BRENDA, the ELIXIR core data resource in 2021: new developments and updates | 10.1093/nar/gkaa1025 | Nucleic Acids Res. 49: D498-D508 2020 | 33211880 | |
| 69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | MicrobeAtlas 1.0 beta | https://microbeatlas.org/ | |||
| 69480 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | https://diaspora-project.de/progress.html#genomes | |||
| 90414 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | StrainInfo: A central database for resolving microbial strain identifiers | 10.60712/SI-ID38407.1 |