Nitrosospira multiformis (DSM 101674, ATCC 25196, C-71)

Name: Nitrosospira multiformis (DSM 101674, ATCC 25196, C-71)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 132056

Type strain

Strain Designation C-71, Nl13

Culture col. no. DSM 101674 ATCC 25196 C-71 Nl13

NCBI tax ID(s) 323848 1231

Special Collections DSMZ

History <- A. Pommerening-Röser, Universität Hamburg, Department of Microbiology and Biotechnology, Germany; Nl13 <- S. W. Watson, Woods Hole Oceanographic Institute, Massachusetts, USA; C-71

Links

Nitrosospira multiformis C-71 is a bacterium that was isolated from soil.

genome sequence 16S sequence Bacteria

Name and taxonomic classification

SI-ID 38407

DSM 101674,ATCC 25196,NCIMB 11849,KCTC 2706

@ref 20215

Last LPSN update 2026-02-09 11:31:37

Domain Bacteria

Phylum Pseudomonadota

Class Betaproteobacteria

Order Spirillales

Family Nitrosomonadaceae

Genus Nitrosospira

Species Nitrosospira multiformis

Full scientific name Nitrosospira multiformis (Watson et al. 1971) Head et al. 1995

Synonyms (1)

Nitrosolobus multiformis

Morphology

Cell morphology

@ref	Gram stain	Confidence
125438	negative	95.994
125439	negative	96.4

Culture and growth conditions

Culture medium

@ref	Name	Growth	Medium link	Composition
23847	MEDIUM FOR AMMONIA OXIDIZING BACTERIA (STRAINS FROM SOIL) (DSMZ Medium 1583)		Medium recipe at MediaDive	Name: MEDIUM FOR AMMONIA OXIDIZING BACTERIA (STRAINS FROM SOIL) (DSMZ Medium 1583) Composition: NaCl 0.584 g/l NH4Cl 0.535 g/l CaCl2 x 2 H2O 0.147 g/l KCl 0.074 g/l KH2PO4 0.054 g/l MgSO4 x 7 H2O 0.049 g/l Cresol red 0.001 g/l FeSO4 x 7 H2O 0.000973 g/l H3BO3 4.9e-05 g/l MnSO4 x 4 H2O 4.5e-05 g/l ZnSO4 x 7 H2O 4.3e-05 g/l (NH4)6Mo7O24 x 4 H2O 3.7e-05 g/l CuSO4 x 5 H2O 2.5e-05 g/l HCl Distilled water

Culture temp

@ref	Growth	Type	Temperature (°C)
23847	positive	growth	28

Physiology and metabolism

Pathway annotation

Computationally determined by

@ref	pathway	enzyme coverage	annotated reactions
66794	methylglyoxal degradation	100	5 of 5
66794	formaldehyde oxidation	100	3 of 3
66794	cis-vaccenate biosynthesis	100	2 of 2
66794	cardiolipin biosynthesis	100	7 of 7
66794	biotin biosynthesis	100	4 of 4
66794	glycogen metabolism	100	5 of 5
66794	acetate fermentation	100	4 of 4
66794	photosynthesis	100	14 of 14
66794	palmitate biosynthesis	100	22 of 22
66794	ppGpp biosynthesis	100	4 of 4
66794	coenzyme A metabolism	100	4 of 4
66794	denitrification	100	2 of 2
66794	adipate degradation	100	2 of 2
66794	ubiquinone biosynthesis	100	7 of 7
66794	CDP-diacylglycerol biosynthesis	100	2 of 2
66794	UDP-GlcNAc biosynthesis	100	3 of 3
66794	aerobactin biosynthesis	100	1 of 1
66794	folate polyglutamylation	100	1 of 1
66794	anapleurotic synthesis of oxalacetate	100	1 of 1
66794	suberin monomers biosynthesis	100	2 of 2
66794	aspartate and asparagine metabolism	88.89	8 of 9
66794	valine metabolism	88.89	8 of 9
66794	chorismate metabolism	88.89	8 of 9
66794	phenylalanine metabolism	84.62	11 of 13
66794	NAD metabolism	83.33	15 of 18
66794	pentose phosphate pathway	81.82	9 of 11
66794	peptidoglycan biosynthesis	80	12 of 15
66794	starch degradation	80	8 of 10
66794	Entner Doudoroff pathway	80	8 of 10
66794	threonine metabolism	80	8 of 10
66794	tetrahydrofolate metabolism	78.57	11 of 14
66794	heme metabolism	78.57	11 of 14
66794	serine metabolism	77.78	7 of 9
66794	vitamin B1 metabolism	76.92	10 of 13
66794	glycogen biosynthesis	75	3 of 4
66794	sulfopterin metabolism	75	3 of 4
66794	6-hydroxymethyl-dihydropterin diphosphate biosynthesis	75	6 of 8
66794	dTDPLrhamnose biosynthesis	75	6 of 8
66794	CMP-KDO biosynthesis	75	3 of 4
66794	cyclohexanol degradation	75	3 of 4
66794	C4 and CAM-carbon fixation	75	6 of 8
66794	isoleucine metabolism	75	6 of 8
66794	purine metabolism	72.34	68 of 94
66794	glycolysis	70.59	12 of 17
66794	urea cycle	69.23	9 of 13
66794	methionine metabolism	69.23	18 of 26
66794	leucine metabolism	69.23	9 of 13
66794	CO2 fixation in Crenarchaeota	66.67	6 of 9
66794	L-lactaldehyde degradation	66.67	2 of 3
66794	cyanate degradation	66.67	2 of 3
66794	d-mannose degradation	66.67	6 of 9
66794	octane oxidation	66.67	2 of 3
66794	acetoin degradation	66.67	2 of 3
66794	citric acid cycle	64.29	9 of 14
66794	glutamate and glutamine metabolism	64.29	18 of 28
66794	proline metabolism	63.64	7 of 11
66794	vitamin B6 metabolism	63.64	7 of 11
66794	ketogluconate metabolism	62.5	5 of 8
66794	gluconeogenesis	62.5	5 of 8
66794	pyrimidine metabolism	62.22	28 of 45
66794	sulfate reduction	61.54	8 of 13
66794	cellulose degradation	60	3 of 5
66794	propionate fermentation	60	6 of 10
66794	hydrogen production	60	3 of 5
66794	lipoate biosynthesis	60	3 of 5
66794	flavin biosynthesis	60	9 of 15
66794	lipid metabolism	58.06	18 of 31
66794	non-pathway related	57.89	22 of 38
66794	isoprenoid biosynthesis	57.69	15 of 26
66794	propanol degradation	57.14	4 of 7
66794	nitrate assimilation	55.56	5 of 9
66794	cysteine metabolism	55.56	10 of 18
66794	alanine metabolism	55.17	16 of 29
66794	histidine metabolism	55.17	16 of 29
66794	phosphatidylethanolamine bioynthesis	53.85	7 of 13
66794	grixazone biosynthesis	50	1 of 2
66794	butanoate fermentation	50	2 of 4
66794	glycine metabolism	50	5 of 10
66794	glutathione metabolism	50	7 of 14
66794	ethanol fermentation	50	1 of 2
66794	arginine metabolism	50	12 of 24
66794	tyrosine metabolism	50	7 of 14
66794	glycolate and glyoxylate degradation	50	3 of 6
66794	lysine metabolism	50	21 of 42
66794	tryptophan metabolism	47.37	18 of 38
66794	cholesterol biosynthesis	45.45	5 of 11
66794	lipid A biosynthesis	44.44	4 of 9
66794	reductive acetyl coenzyme A pathway	42.86	3 of 7
66794	oxidative phosphorylation	41.76	38 of 91
66794	degradation of aromatic, nitrogen containing compounds	41.67	5 of 12
66794	glycine betaine biosynthesis	40	2 of 5
66794	ethylmalonyl-CoA pathway	40	2 of 5
66794	myo-inositol biosynthesis	40	4 of 10
66794	phenol degradation	40	8 of 20
66794	3-chlorocatechol degradation	40	2 of 5
66794	3-phenylpropionate degradation	40	6 of 15
66794	metabolism of amino sugars and derivatives	40	2 of 5
66794	arachidonate biosynthesis	40	2 of 5
66794	polyamine pathway	39.13	9 of 23
66794	phenylpropanoid biosynthesis	38.46	5 of 13
66794	metabolism of disaccharids	36.36	4 of 11
66794	molybdenum cofactor biosynthesis	33.33	3 of 9
66794	IAA biosynthesis	33.33	1 of 3
66794	acetyl CoA biosynthesis	33.33	1 of 3
66794	selenocysteine biosynthesis	33.33	2 of 6
66794	methane metabolism	33.33	1 of 3
66794	arachidonic acid metabolism	33.33	6 of 18
66794	pantothenate biosynthesis	33.33	2 of 6
66794	sphingosine metabolism	33.33	2 of 6
66794	degradation of hexoses	33.33	6 of 18
66794	enterobactin biosynthesis	33.33	1 of 3
66794	(5R)-carbapenem carboxylate biosynthesis	33.33	1 of 3
66794	ascorbate metabolism	31.82	7 of 22
66794	carotenoid biosynthesis	31.82	7 of 22
66794	degradation of sugar alcohols	31.25	5 of 16
66794	coenzyme M biosynthesis	30	3 of 10
66794	degradation of pentoses	28.57	8 of 28
66794	degradation of sugar acids	28	7 of 25
66794	chlorophyll metabolism	27.78	5 of 18
66794	vitamin E metabolism	25	1 of 4
66794	toluene degradation	25	1 of 4
66794	lactate fermentation	25	1 of 4
66794	carnitine metabolism	25	2 of 8
66794	androgen and estrogen metabolism	25	4 of 16
66794	4-hydroxymandelate degradation	22.22	2 of 9
66794	allantoin degradation	22.22	2 of 9

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Environmental	#Terrestrial	#Soil

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent
23847	soil	Paramaribo	Suriname	SUR	Middle and South America

Taxonmaps

69479

Global distribution of 16S sequence AY123807 (>99% sequence identity) for Nitrosospira multiformis from Microbeatlas

Aquatic Samples	320
Soil Samples	1557
Animal Samples	88
Plant Samples	119
Total Samples	2084

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
23847	1	Risk group (German classification)

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
66792	ASM19635v1 assembly for Nitrosospira multiformis ATCC 25196	complete	GCA_000196355	323848.4	637000197	323848	97.42
66792	IMG-taxon 2671180127 annotated assembly for Nitrosospira multiformis ATCC 25196 Nl13	contig	GCA_900108135	323848.18	2671180127	323848	63.52

16S sequences

@ref	Description	Accession	Length	NCBI tax ID
23847	Nitrosospira multiformis 16S ribosomal RNA gene, partial sequence	AY123807	1498	323848
124043	Nitrosospira multiformis gene for 16S rRNA, partial sequence.	AB070984	1371	323848
124043	Nitrosospira multiformis ATCC 25196 16S-23S ribosomal RNA intergenic spacer, partial sequence; and tRNA-Ile and tRNA-Ala genes, complete sequence.	DQ228470	654	1231
124043	Nitrosolobus multiformis (ATCC 25196) 16S ribosomal RNA (16S rRNA) gene.	L35509	1478	1231

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Nitrosospira multiformis ATCC 25196
PATRIC: 323848.4

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	spore_formation	BacteriaNetⓘ	no	88.50	no
125439	motility	BacteriaNetⓘ	yes	50.60	no
125439	gram_stain	BacteriaNetⓘ	negative	96.40	no
125439	oxygen_tolerance	BacteriaNetⓘ	obligate aerobe	88.50	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Nitrosospira multiformis ATCC 25196 ATCC 25196
NCBI: GCA_000196355.1

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	no	95.99	no
125438	anaerobic	anaerobicⓘ	no	87.69	no
125438	aerobic	aerobicⓘ	yes	61.77	no
125438	spore-forming	spore-formingⓘ	no	85.39	no
125438	thermophilic	thermophileⓘ	no	87.71	no
125438	flagellated	motile2+ⓘ	yes	62.04	no

Literature

Topic	Title	Authors	Journal	DOI	Year
	Biological Nitrification Inhibitors with Antagonistic and Synergistic Effects on Growth of Ammonia Oxidisers and Soil Nitrification.	Issifu S, Acharya P, Kaur-Bhambra J, Gubry-Rangin C, Rasche F.	Microb Ecol	10.1007/s00248-024-02456-2	2024
	Wastewater treatment from a science faculty during the COVID-19 pandemic by using ammonium-oxidising and heterotrophic bacteria.	Pedroza-Camacho LD, Ospina-Sanchez PA, Romero-Perdomo FA, Infante-Gonzalez NG, Paredes-Cespedes DM, Quevedo-Hidalgo B, Gutierrez-Romero V, Rivera-Hoyos CM, Pedroza-Rodriguez AM.	3 Biotech	10.1007/s13205-024-03961-4	2024
Genetics	High diversity of nitrifying bacteria and archaea in biofilms from a subsea tunnel.	Kop LFM, Koch H, Dalcin Martins P, Suarez C, Karacic S, Persson F, Wilen BM, Hagelia P, Jetten MSM, Lucker S.	FEMS Microbiol Ecol	10.1093/femsec/fiaf032	2025
Phylogeny	Custom-made medium approach for effective enrichment and isolation of chemolithotrophic iron-oxidizing bacteria.	Uchijima T, Kato S, Tanimoto K, Shiraishi F, Hamamura N, Tokunaga K, Makita H, Kondo M, Ohkuma M, Mitsunobu S.	FEMS Microbiol Ecol	10.1093/femsec/fiaf051	2025
	Assessing the activity of different plant-derived molecules and potential biological nitrification inhibitors on a range of soil ammonia- and nitrite-oxidizing strains.	Kolovou M, Panagiotou D, Susse L, Loiseleur O, Williams S, Karpouzas DG, Papadopoulou ES.	Appl Environ Microbiol	10.1128/aem.01380-23	2023
Phylogeny	Ferriphaselus amnicola strain GF-20, a new iron- and thiosulfate-oxidizing bacterium isolated from a hard rock aquifer.	Garry M, Farasin J, Drevillon L, Quaiser A, Bouchez C, Le Borgne T, Coffinet S, Dufresne A.	FEMS Microbiol Ecol	10.1093/femsec/fiae047	2024
	Dissecting the HGT network of carbon metabolic genes in soil-borne microbiota.	Li L, Liu Y, Xiao Q, Xiao Z, Meng D, Yang Z, Deng W, Yin H, Liu Z.	Front Microbiol	10.3389/fmicb.2023.1173748	2023
Phylogeny	The application of high-throughput sequencing technology to analysis of amoA phylogeny and environmental niche specialisation of terrestrial bacterial ammonia-oxidisers.	Aigle A, Prosser JI, Gubry-Rangin C.	Environ Microbiome	10.1186/s40793-019-0342-6	2019
	Nitrite Oxidizer Activity and Community Are More Responsive Than Their Abundance to Ammonium-Based Fertilizer in an Agricultural Soil.	Ouyang Y, Norton JM.	Front Microbiol	10.3389/fmicb.2020.01736	2020
	A quantitative sequencing method using synthetic internal standards including functional and phylogenetic marker genes.	Koike K, Honda R, Aoki M, Yamamoto-Ikemoto R, Syutsubo K, Matsuura N.	Environ Microbiol Rep	10.1111/1758-2229.13189	2023
Genetics	Genome-centered metagenomics illuminates adaptations of core members to a partial Nitritation-Anammox bioreactor under periodic microaeration.	Shao YH, Wu YW, Naufal M, Wu JH.	Front Microbiol	10.3389/fmicb.2023.1046769	2023
	Important Structural Features of Thiolate-Rich Four-Helix Bundles for Cu(I) Uptake and Removal.	Lee J, Dalton RA, Basle A, Vita N, Dennison C.	Inorg Chem	10.1021/acs.inorgchem.2c04490	2023
	Metabolome fingerprinting reveals the presence of multiple nitrification inhibitors in biomass and root exudates of Thinopyrum intermedium.	Issifu S, Acharya P, Schone J, Kaur-Bhambra J, Gubry-Rangin C, Rasche F.	Plant Environ Interact	10.1002/pei3.70012	2024
Metabolism	Metabolic potential of the imperfect denitrifier Candidatus Desulfobacillus denitrificans in an anammox bioreactor.	Okubo T, Takami H.	Microbiologyopen	10.1002/mbo3.1227	2021
Genetics	The physiological potential of anammox bacteria as revealed by their core genome structure.	Okubo T, Toyoda A, Fukuhara K, Uchiyama I, Harigaya Y, Kuroiwa M, Suzuki T, Murakami Y, Suwa Y, Takami H.	DNA Res	10.1093/dnares/dsaa028	2021
	High Synteny and Sequence Identity between Genomes of Nitrosococcus oceani Strains Isolated from Different Oceanic Gyres Reveals Genome Economization and Autochthonous Clonal Evolution.	Wang L, Lim CK, Klotz MG.	Microorganisms	10.3390/microorganisms8050693	2020
Phylogeny	Quantification and Phylogenetic Analysis of Ammonia Oxidizers on Biofilm Carriers in a Full-Scale Wastewater Treatment Plant.	Tsuchiya Y, Nakagawa T, Takahashi R.	Microbes Environ	10.1264/jsme2.me19140	2020
	Comparative Proteomics of Three Species of Ammonia-Oxidizing Bacteria.	Zorz JK, Kozlowski JA, Stein LY, Strous M, Kleiner M.	Front Microbiol	10.3389/fmicb.2018.00938	2018
Enzymology	An N-acyl homoserine lactone synthase in the ammonia-oxidizing bacterium Nitrosospira multiformis.	Gao J, Ma A, Zhuang X, Zhuang G.	Appl Environ Microbiol	10.1128/aem.03361-13	2014
	Characterisation of bacteria representing a novel Nitrosomonas clade: Physiology, genomics and distribution of missing ammonia oxidizer.	Kikuchi S, Fujitani H, Ishii K, Isshiki R, Sekiguchi Y, Tsuneda S.	Environ Microbiol Rep	10.1111/1758-2229.13158	2023
	Ammonia-oxidizer communities in an agricultural soil treated with contrasting nitrogen sources.	Habteselassie MY, Xu L, Norton JM.	Front Microbiol	10.3389/fmicb.2013.00326	2013
Enzymology	Differential Responses of the Catalytic Efficiency of Ammonia and Nitrite Oxidation to Changes in Temperature.	Taylor AE, Mellbye BL.	Front Microbiol	10.3389/fmicb.2022.817986	2022
Phylogeny	Automatic identification of optimal marker genes for phenotypic and taxonomic groups of microorganisms.	Segev E, Pasternak Z, Ben Sasson T, Jurkevitch E, Gonen M.	PLoS One	10.1371/journal.pone.0195537	2018
	Impairment of a cyanobacterial glycosyltransferase that modifies a pilin results in biofilm development.	Suban S, Sendersky E, Golden SS, Schwarz R.	Environ Microbiol Rep	10.1111/1758-2229.13050	2022
	HgtSIM: a simulator for horizontal gene transfer (HGT) in microbial communities.	Song W, Steensen K, Thomas T.	PeerJ	10.7717/peerj.4015	2017
Enzymology	The [FeFe] hydrogenase of Nyctotherus ovalis has a chimeric origin.	Boxma B, Ricard G, van Hoek AH, Severing E, Moon-van der Staay SY, van der Staay GW, van Alen TA, de Graaf RM, Cremers G, Kwantes M, McEwan NR, Newbold CJ, Jouany JP, Michalowski T, Pristas P, Huynen MA, Hackstein JH.	BMC Evol Biol	10.1186/1471-2148-7-230	2007
	Diverse electron sources support denitrification under hypoxia in the obligate methanotroph Methylomicrobium album strain BG8.	Kits KD, Campbell DJ, Rosana AR, Stein LY.	Front Microbiol	10.3389/fmicb.2015.01072	2015
Phylogeny	Community analysis of betaproteobacterial ammonia-oxidizing bacteria using the amoCAB operon.	Junier P, Kim OS, Junier T, Ahn TS, Imhoff JF, Witzel KP.	Appl Microbiol Biotechnol	10.1007/s00253-009-1923-x	2009
Phylogeny	Highly diverse nirK genes comprise two major clades that harbour ammonium-producing denitrifiers.	Helen D, Kim H, Tytgat B, Anne W.	BMC Genomics	10.1186/s12864-016-2465-0	2016
Metabolism	Comparison among amoA primers suited for quantification and diversity analyses of ammonia-oxidizing bacteria in soil.	Shimomura Y, Morimoto S, Takada Hoshino Y, Uchida Y, Akiyama H, Hayatsu M.	Microbes Environ	10.1264/jsme2.me11230	2012
Metabolism	Distinguishing nitrous oxide production from nitrification and denitrification on the basis of isotopomer abundances.	Sutka RL, Ostrom NE, Ostrom PH, Breznak JA, Gandhi H, Pitt AJ, Li F.	Appl Environ Microbiol	10.1128/aem.72.1.638-644.2006	2006
Metabolism	Aerobic nitrous oxide production through N-nitrosating hybrid formation in ammonia-oxidizing archaea.	Stieglmeier M, Mooshammer M, Kitzler B, Wanek W, Zechmeister-Boltenstern S, Richter A, Schleper C.	ISME J	10.1038/ismej.2013.220	2014
	P2CS: a two-component system resource for prokaryotic signal transduction research.	Barakat M, Ortet P, Jourlin-Castelli C, Ansaldi M, Mejean V, Whitworth DE.	BMC Genomics	10.1186/1471-2164-10-315	2009
Metabolism	Bacterial RuBisCO is required for efficient Bradyrhizobium/Aeschynomene symbiosis.	Gourion B, Delmotte N, Bonaldi K, Nouwen N, Vorholt JA, Giraud E.	PLoS One	10.1371/journal.pone.0021900	2011
Metabolism	Nitrous oxide production and methane oxidation by different ammonia-oxidizing bacteria.	Jiang QQ, Bakken LR.	Appl Environ Microbiol	10.1128/aem.65.6.2679-2684.1999	1999
Genetics	Biotechnological applications of functional metagenomics in the food and pharmaceutical industries.	Coughlan LM, Cotter PD, Hill C, Alvarez-Ordonez A.	Front Microbiol	10.3389/fmicb.2015.00672	2015
	Horizontal gene transfer drives the evolution of Rh50 permeases in prokaryotes.	Matassi G.	BMC Evol Biol	10.1186/s12862-016-0850-6	2017
Enzymology	Phylogenomic analysis and predicted physiological role of the proton-translocating NADH:quinone oxidoreductase (complex I) across bacteria.	Spero MA, Aylward FO, Currie CR, Donohue TJ.	mBio	10.1128/mbio.00389-15	2015
Genetics	Fast and Simple Analysis of MiSeq Amplicon Sequencing Data with MetaAmp.	Dong X, Kleiner M, Sharp CE, Thorson E, Li C, Liu D, Strous M.	Front Microbiol	10.3389/fmicb.2017.01461	2017
Metabolism	Cyanate as an energy source for nitrifiers.	Palatinszky M, Herbold C, Jehmlich N, Pogoda M, Han P, von Bergen M, Lagkouvardos I, Karst SM, Galushko A, Koch H, Berry D, Daims H, Wagner M.	Nature	10.1038/nature14856	2015
	Group II intron-like reverse transcriptases function in double-strand break repair.	Park SK, Mohr G, Yao J, Russell R, Lambowitz AM.	Cell	10.1016/j.cell.2022.08.014	2022
Metabolism	Effects of aeration cycles on nitrifying bacterial populations and nitrogen removal in intermittently aerated reactors.	Mota C, Head MA, Ridenoure JA, Cheng JJ, de Los Reyes FL.	Appl Environ Microbiol	10.1128/aem.71.12.8565-8572.2005	2005
	Comprehensive prediction of chromosome dimer resolution sites in bacterial genomes.	Kono N, Arakawa K, Tomita M.	BMC Genomics	10.1186/1471-2164-12-19	2011
Genetics	Identification and characterization of new LuxR/LuxI-type quorum sensing systems from metagenomic libraries.	Hao Y, Winans SC, Glick BR, Charles TC.	Environ Microbiol	10.1111/j.1462-2920.2009.02049.x	2010
	Nitrosospira sp. Govern Nitrous Oxide Emissions in a Tropical Soil Amended With Residues of Bioenergy Crop.	Lourenco KS, Cassman NA, Pijl AS, van Veen JA, Cantarella H, Kuramae EE.	Front Microbiol	10.3389/fmicb.2018.00674	2018
Enzymology	Urease-encoding genes in ammonia-oxidizing bacteria.	Koper TE, El-Sheikh AF, Norton JM, Klotz MG.	Appl Environ Microbiol	10.1128/aem.70.4.2342-2348.2004	2004
Metabolism	Evolution and functional characterization of the RH50 gene from the ammonia-oxidizing bacterium Nitrosomonas europaea.	Cherif-Zahar B, Durand A, Schmidt I, Hamdaoui N, Matic I, Merrick M, Matassi G.	J Bacteriol	10.1128/jb.01089-07	2007
	Organic Matter Regulates Ammonia-Oxidizing Bacterial and Archaeal Communities in the Surface Sediments of Ctenopharyngodon idellus Aquaculture Ponds.	Dai L, Liu C, Yu L, Song C, Peng L, Li X, Tao L, Li G.	Front Microbiol	10.3389/fmicb.2018.02290	2018
Metabolism	Genes and pathways for CO2 fixation in the obligate, chemolithoautotrophic acidophile, Acidithiobacillus ferrooxidans, carbon fixation in A. ferrooxidans.	Esparza M, Cardenas JP, Bowien B, Jedlicki E, Holmes DS.	BMC Microbiol	10.1186/1471-2180-10-229	2010
Metabolism	Genome Analysis of the Biotechnologically Relevant Acidophilic Iron Oxidising Strain JA12 Indicates Phylogenetic and Metabolic Diversity within the Novel Genus "Ferrovum".	Ullrich SR, Poehlein A, Tischler JS, Gonzalez C, Ossandon FJ, Daniel R, Holmes DS, Schlomann M, Muhling M.	PLoS One	10.1371/journal.pone.0146832	2016
Genetics	Proteomics and comparative genomics of Nitrososphaera viennensis reveal the core genome and adaptations of archaeal ammonia oxidizers.	Kerou M, Offre P, Valledor L, Abby SS, Melcher M, Nagler M, Weckwerth W, Schleper C.	Proc Natl Acad Sci U S A	10.1073/pnas.1601212113	2016
	The role of horizontal transfer in the evolution of a highly variable lipopolysaccharide biosynthesis locus in xanthomonads that infect rice, citrus and crucifers.	Patil PB, Bogdanove AJ, Sonti RV.	BMC Evol Biol	10.1186/1471-2148-7-243	2007
Enzymology	Chemolithotrophic primary production in a subglacial ecosystem.	Boyd ES, Hamilton TL, Havig JR, Skidmore ML, Shock EL.	Appl Environ Microbiol	10.1128/aem.01956-14	2014
	Biochar decelerates soil organic nitrogen cycling but stimulates soil nitrification in a temperate arable field trial.	Prommer J, Wanek W, Hofhansl F, Trojan D, Offre P, Urich T, Schleper C, Sassmann S, Kitzler B, Soja G, Hood-Nowotny RC.	PLoS One	10.1371/journal.pone.0086388	2014
	The Pseudomonas quorum-sensing regulator RsaL belongs to the tetrahelical superclass of H-T-H proteins.	Rampioni G, Polticelli F, Bertani I, Righetti K, Venturi V, Zennaro E, Leoni L.	J Bacteriol	10.1128/jb.01552-06	2007
	A complete set of flagellar genes acquired by horizontal transfer coexists with the endogenous flagellar system in Rhodobacter sphaeroides.	Poggio S, Abreu-Goodger C, Fabela S, Osorio A, Dreyfus G, Vinuesa P, Camarena L.	J Bacteriol	10.1128/jb.01681-06	2007
Phylogeny	Across bacterial phyla, distantly-related genomes with similar genomic GC content have similar patterns of amino acid usage.	Lightfield J, Fram NR, Ely B.	PLoS One	10.1371/journal.pone.0017677	2011
Metabolism	The ammonia monooxygenase structural gene amoA as a functional marker: molecular fine-scale analysis of natural ammonia-oxidizing populations.	Rotthauwe JH, Witzel KP, Liesack W.	Appl Environ Microbiol	10.1128/aem.63.12.4704-4712.1997	1997
Metabolism	Diversity and transcription of proteases involved in the maturation of hydrogenases in Nostoc punctiforme ATCC 29133 and Nostoc sp. strain PCC 7120.	Devine E, Holmqvist M, Stensjo K, Lindblad P.	BMC Microbiol	10.1186/1471-2180-9-53	2009
Enzymology	Phylogeny and functional expression of ribulose 1,5-bisphosphate carboxylase/oxygenase from the autotrophic ammonia-oxidizing bacterium Nitrosospira sp. isolate 40KI.	Utaker JB, Andersen K, Aakra A, Moen B, Nes IF.	J Bacteriol	10.1128/jb.184.2.468-478.2002	2002
Metabolism	Nitrosopumilus maritimus genome reveals unique mechanisms for nitrification and autotrophy in globally distributed marine crenarchaea.	Walker CB, de la Torre JR, Klotz MG, Urakawa H, Pinel N, Arp DJ, Brochier-Armanet C, Chain PS, Chan PP, Gollabgir A, Hemp J, Hugler M, Karr EA, Konneke M, Shin M, Lawton TJ, Lowe T, Martens-Habbena W, Sayavedra-Soto LA, Lang D, Sievert SM, Rosenzweig AC, Manning G, Stahl DA.	Proc Natl Acad Sci U S A	10.1073/pnas.0913533107	2010
Phylogeny	Effect of lake trophic status and rooted macrophytes on community composition and abundance of ammonia-oxidizing prokaryotes in freshwater sediments.	Herrmann M, Saunders AM, Schramm A.	Appl Environ Microbiol	10.1128/aem.02806-08	2009
Metabolism	Use of molecular and isotopic techniques to monitor the response of autotrophic ammonia-oxidizing populations of the beta subdivision of the class proteobacteria in arable soils to nitrogen fertilizer.	Mendum TA, Sockett RE, Hirsch PR.	Appl Environ Microbiol	10.1128/aem.65.9.4155-4162.1999	1999
	Bioinformatic characterization of the trimeric intracellular cation-specific channel protein family.	Silverio AL, Saier MH.	J Membr Biol	10.1007/s00232-011-9364-8	2011
	Distribution of genes encoding nucleoid-associated protein homologs in plasmids.	Takeda T, Yun CS, Shintani M, Yamane H, Nojiri H.	Int J Evol Biol	10.4061/2011/685015	2011
	Annotation of Protein Domains Reveals Remarkable Conservation in the Functional Make up of Proteomes Across Superkingdoms.	Nasir A, Naeem A, Khan MJ, Nicora HD, Caetano-Anolles G.	Genes (Basel)	10.3390/genes2040869	2011
	Application of molecular biological techniques to a seasonal study of ammonia oxidation in a eutrophic freshwater lake	Hastings RC, Saunders JR, Hall GH, Pickup RW, McCarthy AJ.	Appl Environ Microbiol	10.1128/aem.64.10.3674-3682.1998	1998
Enzymology	Characterization of microbial communities and composition in constructed dairy wetland wastewater effluent.	Ibekwe AM, Grieve CM, Lyon SR.	Appl Environ Microbiol	10.1128/aem.69.9.5060-5069.2003	2003
Metabolism	Culture-independent techniques for rapid detection of bacteria associated with loss of chloramine residual in a drinking water system.	Hoefel D, Monis PT, Grooby WL, Andrews S, Saint CP.	Appl Environ Microbiol	10.1128/aem.71.11.6479-6488.2005	2005
Genetics	Adaptations to submarine hydrothermal environments exemplified by the genome of Nautilia profundicola.	Campbell BJ, Smith JL, Hanson TE, Klotz MG, Stein LY, Lee CK, Wu D, Robinson JM, Khouri HM, Eisen JA, Cary SC.	PLoS Genet	10.1371/journal.pgen.1000362	2009
Metabolism	ABC transporters involved in export of cell surface glycoconjugates.	Cuthbertson L, Kos V, Whitfield C.	Microbiol Mol Biol Rev	10.1128/mmbr.00009-10	2010
	Complete genome of Nitrosospira briensis C-128, an ammonia-oxidizing bacterium from agricultural soil.	Rice MC, Norton JM, Valois F, Bollmann A, Bottomley PJ, Klotz MG, Laanbroek HJ, Suwa Y, Stein LY, Sayavedra-Soto L, Woyke T, Shapiro N, Goodwin LA, Huntemann M, Clum A, Pillay M, Kyrpides N, Varghese N, Mikhailova N, Markowitz V, Palaniappan K, Ivanova N, Stamatis D, Reddy TB, Ngan CY, Daum C	Stand Genomic Sci	10.1186/s40793-016-0168-4	2016
Metabolism	Pathways and key intermediates required for obligate aerobic ammonia-dependent chemolithotrophy in bacteria and Thaumarchaeota.	Kozlowski JA, Stieglmeier M, Schleper C, Klotz MG, Stein LY	ISME J	10.1038/ismej.2016.2	2016
Metabolism	Effects of nitrite on ammonia-oxidizing activity and gene regulation in three ammonia-oxidizing bacteria.	Cua LS, Stein LY	FEMS Microbiol Lett	10.1111/j.1574-6968.2011.02277.x	2011
Pathogenicity	Various physico-chemical stress factors cause prophage induction in Nitrosospira multiformis 25196--an ammonia oxidizing bacteria.	Choi J, Kotay SM, Goel R	Water Res	10.1016/j.watres.2010.04.040	2010
Genetics	Complete genome sequence of Nitrosospira multiformis, an ammonia-oxidizing bacterium from the soil environment.	Norton JM, Klotz MG, Stein LY, Arp DJ, Bottomley PJ, Chain PS, Hauser LJ, Land ML, Larimer FW, Shin MW, Starkenburg SR	Appl Environ Microbiol	10.1128/AEM.02722-07	2008
Phylogeny	Characteristics of an ammonia-oxidizing bacterium with a plasmid isolated from alkaline soils and its phylogenetic relationship.	Takahashi R, Ohishi M, Ohshima M, Saitoh M, Omata K, Tokuyama T	J Biosci Bioeng	10.1263/jbb.92.232	2001
Metabolism	Susceptibility of ammonia-oxidizing bacteria to nitrification inhibitors.	Matsuba D, Takazaki H, Sato Y, Takahashi R, Tokuyama T, Wakabayashi K	Z Naturforsch C J Biosci	10.1515/znc-2003-3-424	2003
Phylogeny	Comparative analysis of gene sequences encoding ammonia monooxygenase of Nitrosospira sp. AHB1 and Nitrosolobus multiformis C-71.	Rotthauwe JH, de Boer W, Liesack W	FEMS Microbiol Lett	10.1111/j.1574-6968.1995.tb07873.x	1995
Phylogeny	Evolutionary relationships among ammonia- and nitrite-oxidizing bacteria.	Teske A, Alm E, Regan JM, Toze S, Rittmann BE, Stahl DA	J Bacteriol	10.1128/jb.176.21.6623-6630.1994	1994
Phylogeny	Usitatibacter rugosus gen. nov., sp. nov. and Usitatibacter palustris sp. nov., novel members of Usitatibacteraceae fam. nov. within the order Nitrosomonadales isolated from soil.	Vieira S, Huber KJ, Neumann-Schaal M, Geppert A, Luckner M, Wanner G, Overmann J	Int J Syst Evol Microbiol	10.1099/ijsem.0.004631	2021
Phylogeny	Nitrosospira lacus sp. nov., a psychrotolerant, ammonia-oxidizing bacterium from sandy lake sediment.	Urakawa H, Garcia JC, Nielsen JL, Le VQ, Kozlowski JA, Stein LY, Lim CK, Pommerening-Roser A, Martens-Habbena W, Stahl DA, Klotz MG	Int J Syst Evol Microbiol	10.1099/ijs.0.070789-0	2014

References

#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#23847	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 101674
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#66794	Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg: BRENDA, the ELIXIR core data resource in 2021: new developments and updates. Nucleic Acids Res. 49: D498 - D508 2020 ( DOI 10.1093/nar/gkaa1025 , PubMed 33211880 )
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#124043	Isabel Schober, Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Nitrosospira multiformis (DSM 101674, ATCC 25196, C-71)

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