Strain identifier

BacDive ID: 13192

Type strain: Yes

Species: Actinoalloteichus caeruleus

Strain Designation: Y388=As 4.1159, Y-388

Strain history: CIP <- 2000, IFO <- T. Tamura: strain AS 4.1159 <- Z.H. Liu, IMAS: strain Y-388

NCBI tax ID(s): 2893586 (species)

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General

@ref: 11368

BacDive-ID: 13192

DSM-Number: 43889

keywords: genome sequence, 16S sequence, Bacteria, obligate aerobe, spore-forming, Gram-positive

description: Actinoalloteichus caeruleus Y388=As 4.1159 is an obligate aerobe, spore-forming, Gram-positive bacterium that was isolated from soil.

NCBI tax id

  • NCBI tax id: 2893586
  • Matching level: species

strain history

@refhistory
11368<- JCM <- Z. Liu, Y388=As 4.1159
67770Z. Liu Y388.
118827CIP <- 2000, IFO <- T. Tamura: strain AS 4.1159 <- Z.H. Liu, IMAS: strain Y-388

doi: 10.13145/bacdive13192.20240916.9.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Pseudonocardiales
  • family: Pseudonocardiaceae
  • genus: Actinoalloteichus
  • species: Actinoalloteichus caeruleus
  • full scientific name: Actinoalloteichus caeruleus (Baldacci 1944) Teo et al. 2021

  • synonyms

    @refsynonym
    20215Actinomyces caeruleus
    20215Actinoalloteichus cyanogriseus
    20215Streptomyces caeruleus

@ref: 11368

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Pseudonocardiaceae

genus: Actinoalloteichus

species: Actinoalloteichus cyanogriseus

full scientific name: Actinoalloteichus cyanogriseus Tamura et al. 2000

strain designation: Y388=As 4.1159, Y-388

type strain: yes

Morphology

cell morphology

@refgram stainmotilityconfidence
118827positiveno
69480positive91.608
69480no91.082

colony morphology

  • @ref: 118827

multimedia

@refmultimedia contentcaptionintellectual property rights
11368https://www.dsmz.de/microorganisms/photos/DSM_43889.jpgMedium 535 28°C© Leibniz-Institut DSMZ
66793EM_DSM_43889_1.jpgelectron microscopic image© HZI/Manfred Rohde
66793EM_DSM_43889_2.jpgelectron microscopic image© HZI/Manfred Rohde
66793EM_DSM_43889_3.jpgelectron microscopic image© HZI/Manfred Rohde
66793EM_DSM_43889_4.jpgelectron microscopic image© HZI/Manfred Rohde
66793EM_DSM_43889_5.jpgelectron microscopic image© HZI/Manfred Rohde

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
11368GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)yeshttps://mediadive.dsmz.de/medium/65Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water
11368TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535)yeshttps://mediadive.dsmz.de/medium/535Name: TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Distilled water
39572MEDIUM 366 - for Actinoalloteichus cyanogriseusyesDistilled water make up to (1000.000 ml);Agar (20.000 g);Glucose (4.000g);Yeast extract (4.000 g);Malt extract (10.000 g)
118827CIP Medium 366yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=366

culture temp

@refgrowthtypetemperature
11368positivegrowth28
39572positivegrowth30
67770positivegrowth28
118827positivegrowth25-41
118827nogrowth10
118827nogrowth45

Physiology and metabolism

oxygen tolerance

@refoxygen toleranceconfidence
118827obligate aerobe
69480aerobe90.39

spore formation

  • @ref: 69481
  • spore formation: yes
  • confidence: 99

observation

  • @ref: 67770
  • observation: quinones: MK-9(H4)

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6836925115malate+assimilation
6836927689decanoate-assimilation
6836924265gluconate+assimilation
6836917306maltose+assimilation
6836916899D-mannitol+assimilation
683695291gelatin+hydrolysis
683694853esculin+hydrolysis
6836916199urea-hydrolysis
6836929016arginine-hydrolysis
6836917634D-glucose-fermentation
6836927897tryptophan-energy source
6836917632nitrate-reduction
1188274853esculin-hydrolysis
118827606565hippurate+hydrolysis
11882717632nitrate-reduction
11882716301nitrite-reduction

antibiotic resistance

  • @ref: 118827
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: yes
  • is resistant: no

metabolite production

@refChebi-IDmetaboliteproduction
6836935581indoleno
11882735581indoleno

metabolite tests

@refChebi-IDmetaboliteindole testvoges-proskauer-testmethylred-test
6836935581indole-
11882715688acetoin-
11882717234glucose-

enzymes

@refvalueactivityec
68369gelatinase+
68369beta-glucosidase+3.2.1.21
68369urease-3.5.1.5
68369arginine dihydrolase-3.5.3.6
118827oxidase-
118827beta-galactosidase+3.2.1.23
118827alcohol dehydrogenase-1.1.1.1
118827gelatinase+/-
118827amylase-
118827DNase-
118827caseinase-3.4.21.50
118827catalase+1.11.1.6
118827tween esterase-
118827gamma-glutamyltransferase+2.3.2.2
118827lysine decarboxylase-4.1.1.18
118827ornithine decarboxylase-4.1.1.17
118827phenylalanine ammonia-lyase-4.3.1.24
118827tryptophan deaminase-
118827urease-3.5.1.5
68382leucine arylamidase+3.4.11.1
68382cystine arylamidase-3.4.11.3
68382acid phosphatase-3.1.3.2
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase+3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase+3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
118827-+++++---+-+++-+-+--
11368-----++-++/----+/--+-+--

API 20NE

@refNO3TRPGLU_ FermADH ArgUREESCGELPNPGGLU_ AssimARAMNEMANNAGMALGNTCAPADIMLTCITPAC
11368-----++++/-+/-+/-++/-++-+/-++-

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
118827---+-----++----+--+---+---+------+--+---------------+----+--+--------------+---++--------+++-------

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
11368soilYunnan ProvinceChinaCHNAsia
67770SoilYunnan ProvinceChinaCHNAsia
118827Environment, SoilYunnanChinaCHNAsia

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Terrestrial
  • Cat3: #Soil

taxonmaps

  • @ref: 69479
  • File name: preview.99_5097.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_10;96_1336;97_3030;98_3805;99_5097&stattab=map
  • Last taxonomy: Actinoalloteichus
  • 16S sequence: AB006178
  • Sequence Identity:
  • Total samples: 1669
  • soil counts: 1111
  • aquatic counts: 125
  • animal counts: 269
  • plant counts: 164

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
113681Risk group (German classification)
1188271Risk group (French classification)

Sequence information

16S sequences

  • @ref: 20218
  • description: Actinoalloteichus caeruleus gene for 16S ribosomal RNA, partial sequence
  • accession: AB006178
  • length: 1468
  • database: nuccore
  • NCBI tax ID: 65497

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Actinoalloteichus cyanogriseus DSM 438891120930.3wgspatric1120930
66792Actinoalloteichus cyanogriseus DSM 438892524614639draftimg1120930
66792Actinoalloteichus cyanogriseus DSM 438892585427525draftimg1120930
67770Actinoalloteichus caeruleus DSM 43889GCA_000429185contigncbi1120930

GC content

@refGC-contentmethod
6777072.5high performance liquid chromatography (HPLC)
6777069.54thermal denaturation, midpoint method (Tm)

Genome-based predictions

predictions

@reftraitmodeldescriptionpredictionconfidencetraining_data
69481spore-formingspore-formingAbility to form endo- or exosporesyes99no
69480gram-positivegram-positivePositive reaction to Gram-stainingyes91.608no
69480anaerobicanaerobicAbility to grow under anoxygenic conditions (including facultative anaerobes)no97.178yes
69480spore-formingspore-formingAbility to form endo- or exosporesyes72.875no
69480aerobicaerobicAbility to grow under oxygenic conditions (including facultative aerobes)yes90.39yes
69480thermophilicthermophileAbility to grow at temperatures above or equal to 45°Cno93.922no
69480flagellatedmotile2+Ability to perform flagellated movementno91.082no

External links

@ref: 11368

culture collection no.: DSM 43889, IFO 14455, IMET 43868, JCM 6095, NBRC 14455, NRRL B-16252, CGMCC 4.1159, CIP 106755, KCCM 12121, VKM Ac-1271, AS 4.1159

straininfo link

  • @ref: 82400
  • straininfo: 297066

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny10939647Actinoalloteichus cyanogriseus gen. nov., sp. nov.Tamura T, Zhiheng L, Yamei Z, Hatano KInt J Syst Evol Microbiol10.1099/00207713-50-4-14352000Alanine/analysis, Cell Wall/chemistry, DNA, Bacterial/genetics, Diaminopimelic Acid, Glutamic Acid/analysis, Gram-Positive Bacteria/chemistry/*classification/genetics, Molecular Sequence Data, Phylogeny, RNA, Ribosomal, 16S/analysis, Species Specificity, Vitamin KGenetics
Phylogeny19060064Reclassification of Streptomyces caeruleus as a synonym of Actinoalloteichus cyanogriseus and reclassification of Streptomyces spheroides and Streptomyces laceyi as later synonyms of Streptomyces niveus.Tamura T, Ishida Y, Otoguro M, Hatano K, Labeda D, Price NP, Suzuki KInt J Syst Evol Microbiol10.1099/ijs.0.65560-02008Actinomycetales/chemistry/*classification/genetics, Molecular Sequence Data, RNA, Ribosomal, 16S/genetics, Sequence Homology, Nucleic Acid, Species Specificity, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, Streptomyces/chemistry/*classification/geneticsGenetics

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
11368Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 43889)https://www.dsmz.de/collection/catalogue/details/culture/DSM-43889
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
39572Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/18920
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
66793Mukherjee et al.10.1038/nbt.3886GEBA: 1,003 reference genomes of bacterial and archaeal isolates expand coverage of the tree of life 35: 676-683 201728604660
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68369Automatically annotated from API 20NE
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
82400Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID297066.1StrainInfo: A central database for resolving microbial strain identifiers
118827Curators of the CIPCollection of Institut Pasteur (CIP 106755)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20106755