General

@ref: 22788

BacDive-ID: 131561

DSM-Number: 26011

keywords: 16S sequence, Bacteria

description: Thermorudis pharmacophila Wkt50.2 is a bacterium that was isolated from geothermal soil.

NCBI tax id

• NCBI tax id: 1167092
• Matching level: species

strain history

• @ref: 22788
• history: <- M. Stott, GNS Science, Taupo, New Zealand; Wkt50.2 <- X. Morgan

doi: 10.13145/bacdive131561.20240510.9

Name and taxonomic classification

LPSN

• @ref: 20215
• description: domain/bacteria
• keyword: phylum/thermomicrobiota
• domain: Bacteria
• phylum: Thermomicrobiota
• class: Thermomicrobia
• order: Thermomicrobiales
• family: Thermomicrobiaceae
• genus: Thermorudis
• species: Thermorudis pharmacophila
• full scientific name: Thermorudis pharmacophila Houghton et al. 2015

@ref: 22788

domain: Bacteria

phylum: Thermomicrobia

class: Thermomicrobia

order: Thermomicrobia, not assigned to order

family: Thermomicrobiaceae

genus: Thermorudis

species: Thermorudis pharmacophila

full scientific name: Thermorudis pharmacophila Houghton et al. 2015

strain designation: Wkt50.2

type strain: yes

Culture and growth conditions

culture medium

• @ref: 22788
• name: MODIFIED CASTENHOLZ MEDIUM (DSMZ Medium 1470)
• growth: yes
• link: https://mediadive.dsmz.de/medium/1470
• composition: Name: MODIFIED CASTENHOLZ MEDIUM (DSMZ Medium 1470) Composition: Tryptone 5.0 g/l Peptone 5.0 g/l Sucrose 2.0 g/l NaNO3 0.689 g/l Na2HPO4 x 2 H2O 0.111 g/l KNO3 0.103 g/l MgSO4 x 7 H2O 0.1 g/l Nitrilotriacetic acid 0.1 g/l CaSO4 x 2 H2O 0.06 g/l FeCl3 x 6 H2O 0.028 g/l NaCl 0.008 g/l MnSO4 x H2O 2.2e-06 g/l H3BO3 5e-07 g/l ZnSO4 x 7 H2O 5e-07 g/l CoCl2 x 6 H2O 4.6e-08 g/l Na2MoO4 x 2 H2O 2.5e-08 g/l CuSO4 x 5 H2O 1.6e-08 g/l H2SO4 Distilled water

culture temp

• @ref: 22788
• growth: positive
• type: growth
• temperature: 68

Isolation, sampling and environmental information

isolation

• @ref: 22788
• sample type: geothermal soil
• geographic location: Waikite
• country: New Zealand
• origin.country: NZL
• continent: Australia and Oceania

isolation source categories

taxonmaps

• @ref: 69479
• File name: preview.99_7545.png
• url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_651;96_3521;97_4320;98_5531;99_7545&stattab=map
• Last taxonomy: Thermorudis
• 16S sequence: HE794997
• Sequence Identity:
• Total samples: 1187
• soil counts: 413
• aquatic counts: 208
• animal counts: 541
• plant counts: 25

Safety information

risk assessment

• @ref: 22788
• biosafety level: 1
• biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

• @ref: 22788
• description: Thermomicrobiaceae bacterium WKT50.2 partial 16S rRNA gene, type strain WKT50.2T
• accession: HE794997
• length: 1356
• database: nuccore
• NCBI tax ID: 1167092

GC content

• @ref: 22788
• GC-content: 58.4
• method: high performance liquid chromatography (HPLC)

External links

@ref: 22788

culture collection no.: DSM 26011, ICMP 20042

straininfo link

• @ref: 89975
• straininfo: 401965

literature

• topic: Phylogeny
• Pubmed-ID: 26374291
• title: Thermorudis pharmacophila sp. nov., a novel member of the class Thermomicrobia isolated from geothermal soil, and emended descriptions of Thermomicrobium roseum, Thermomicrobium carboxidum, Thermorudis peleae and Sphaerobacter thermophilus.
• authors: Houghton KM, Morgan XC, Lagutin K, MacKenzie AD, Vyssotskii M, Mitchell KA, McDonald IR, Morgan HW, Power JF, Moreau JW, Hanssen E, Stott MB
• journal: Int J Syst Evol Microbiol
• DOI: 10.1099/ijsem.0.000598
• year: 2015
• mesh: Bacterial Typing Techniques, Base Composition, Chloroflexi/*classification/genetics/isolation & purification, DNA, Bacterial/genetics, Fatty Acids/chemistry, Hot Springs, Hot Temperature, Molecular Sequence Data, New Zealand, Nucleic Acid Hybridization, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Soil Microbiology, Vitamin K 2/analogs & derivatives/chemistry
• topic2: Stress

Reference