Strain identifier

BacDive ID: 131551

Type strain: Yes

Species: Actinomadura algeriensis

Strain Designation: ACD1

Strain history: <- N. Sabaou, Alger, Algeria; ACD1

NCBI tax ID(s): 1679523 (species)

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General

@ref: 22778

BacDive-ID: 131551

DSM-Number: 46744

keywords: genome sequence, 16S sequence, Bacteria, spore-forming, mesophilic, Gram-positive

description: Actinomadura algeriensis ACD1 is a spore-forming, mesophilic, Gram-positive bacterium that builds an aerial mycelium and was isolated from Saharan soil .

NCBI tax id

  • NCBI tax id: 1679523
  • Matching level: species

strain history

  • @ref: 22778
  • history: <- N. Sabaou, Alger, Algeria; ACD1

doi: 10.13145/bacdive131551.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Streptosporangiales
  • family: Thermomonosporaceae
  • genus: Actinomadura
  • species: Actinomadura algeriensis
  • full scientific name: Actinomadura algeriensis Lahoum et al. 2017

@ref: 22778

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Thermomonosporaceae

genus: Actinomadura

species: Actinomadura algeriensis

full scientific name: Actinomadura algeriensis Lahoum et al. 2017

strain designation: ACD1

type strain: yes

Morphology

cell morphology

  • @ref: 69480
  • gram stain: positive
  • confidence: 100

colony morphology

@refcolony colormedium used
69471Brown red (3011)ISP 7
69471Black red (3007)ISP 2
69471Pastel violet (4009)ISP 6
69471Purple red (3004), beige (1001)suter without tyrosine
69471Purple red (3004), salmon pink (3022)suter with tyrosine
69471Purple red (3004), strawberry red (3018)ISP 5
69471Ruby red (3003)ISP 4
69471Wine red (3005)ISP 3

multicellular morphology

@refforms multicellular complexcomplex namemedium namecomplex color
69471noAerial myceliumISP 2
69471yesAerial myceliumISP 3Traffic white (9016), agate grey (7038)
69471yesAerial myceliumISP 4Pure white (9010)
69471yesAerial myceliumISP 5Platinum grey (7036), traffic white (9016)
69471noAerial myceliumISP 6
69471yesAerial myceliumISP 7Traffic white (9016)
69471noAerial myceliumsuter with tyrosine
69471noAerial myceliumsuter without tyrosine

pigmentation

@refproductionnamecolor
69471noMelanin
69471yessoluble pigmentPurple red (3004), antique pink (3014), ruby red (3003), beige red (3012)

multimedia

  • @ref: 22778
  • multimedia content: https://www.dsmz.de/microorganisms/photos/DSM_46744.jpg
  • caption: Medium 553 28°C
  • intellectual property rights: © Leibniz-Institut DSMZ

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
22778GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)yeshttps://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium65.pdf
22778GPHF-MEDIUM (DSMZ Medium 553)yeshttps://mediadive.dsmz.de/medium/553Name: GPHF-MEDIUM (DSMZ Medium 553) Composition: Agar 20.0 g/l Glucose 10.0 g/l Beef extract 5.0 g/l Yeast extract 5.0 g/l Casein peptone 5.0 g/l CaCl2 x 2 H2O 0.74 g/l Distilled water

culture temp

  • @ref: 22778
  • growth: positive
  • type: growth
  • temperature: 28
  • range: mesophilic

Physiology and metabolism

spore formation

@refspore formationconfidence
69481yes100
69480yes100

halophily

  • @ref: 69471
  • salt: NaCl
  • growth: positive
  • tested relation: growth
  • concentration: 0-2.5 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6947122599arabinose+growth
6947162968cellulose-growth
6947128757fructose+growth
6947117234glucose+growth
6947117268inositol-growth
6947137684mannose+growth
6947116634raffinose-growth
6947126546rhamnose+growth
6947117992sucrose-growth
6947118222xylose+growth
6837917632nitrate-reduction
683794853esculin+hydrolysis
6837916199urea-hydrolysis
683795291gelatin+hydrolysis
6837917634D-glucose-fermentation
6837916988D-ribose-fermentation
6837965327D-xylose-fermentation
6837916899D-mannitol-fermentation
6837917306maltose-fermentation
6837917716lactose-fermentation
6837917992sucrose-fermentation
6837928087glycogen-fermentation

enzymes

@refvalueactivityec
68379gelatinase+
68379urease-3.5.1.5
68379beta-glucosidase+3.2.1.21
68379N-acetyl-beta-glucosaminidase-3.2.1.52
68379alpha-glucosidase-3.2.1.20
68379beta-galactosidase-3.2.1.23
68379beta-glucuronidase-3.2.1.31
68379alkaline phosphatase+3.1.3.1
68379pyrrolidonyl arylamidase+3.4.19.3
68379pyrazinamidase-3.5.1.B15
68382alkaline phosphatase+3.1.3.1
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase+
68382alpha-chymotrypsin+3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382beta-glucosidase+3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-fucosidase-3.2.1.51

API coryne

@refNITPYZPYRAPALbeta GURbeta GALalpha GLUbeta NAGESCUREGELGLURIBXYLMANMALLACSACGLYG
69471--++----+-+--------

API zym

@refAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
69471++/-+-+++/-+/-+++---+/-+-+/--

Isolation, sampling and environmental information

isolation

  • @ref: 22778
  • sample type: Saharan soil (22° 47' N, 5° 31' E)
  • geographic location: Sahara, Tamanrasset province, Hoggar
  • country: Algeria
  • origin.country: DZA
  • continent: Africa
  • latitude: 22.7833
  • longitude: 5.5167

isolation source categories

Cat1Cat2Cat3
#Environmental#Terrestrial#Desert
#Environmental#Terrestrial#Soil
#Climate#Hot#Arid

Safety information

risk assessment

  • @ref: 22778
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 22778
  • description: Actinomadura algeriensis strain ACD1 16S ribosomal RNA gene, partial sequence
  • accession: KT259320
  • length: 1446
  • database: ena
  • NCBI tax ID: 1679523

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Actinomadura algeriensis DSM 46744GCA_014873935contigncbi1679523
66792Actinomadura algeriensis strain DSM 467441679523.3wgspatric1679523
66792Actinomadura algeriensis DSM 467442870812040draftimg1679523

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingyes100no
motileno91.314no
gram-positiveyes85.997no
anaerobicno98.296no
aerobicyes87.102no
halophileno82.486no
spore-formingyes93.426no
glucose-utilyes88.653yes
flagellatedno98.398no
thermophileno97.01yes
glucose-fermentno90.965no

External links

@ref: 22778

culture collection no.: DSM 46744, CECT 8841

straininfo link

  • @ref: 89965
  • straininfo: 402801

literature

  • topic: Phylogeny
  • Pubmed-ID: 26542839
  • title: Actinomadura algeriensis sp. nov., an actinobacterium isolated from Saharan soil.
  • authors: Lahoum A, Bouras N, Mathieu F, Schumann P, Sproer C, Klenk HP, Sabaou N
  • journal: Antonie Van Leeuwenhoek
  • DOI: 10.1007/s10482-015-0617-x
  • year: 2015
  • mesh: Actinomycetales/*classification/genetics/*isolation & purification/metabolism, Algeria, Cell Wall/metabolism, Cluster Analysis, DNA, Bacterial/genetics, DNA, Ribosomal/genetics, Diaminopimelic Acid/metabolism, Fatty Acids/analysis, Molecular Sequence Data, Mycelium/cytology, Nucleic Acid Hybridization, Phospholipids/metabolism, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Soil Microbiology, Spores, Bacterial/cytology, Vitamin K 2/metabolism
  • topic2: Metabolism

Reference

@idauthorstitledoi/urlcatalogue
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
22778Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-46744Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 46744)
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
68379Automatically annotated from API Coryne
68382Automatically annotated from API zym
69471Wink, J.Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweighttps://cdn.dsmz.de/wink/DSM%2046744.pdf
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. MünchPredictions based on the model GenomeNet Sporulation v. 110.21203/rs.3.rs-2527258/v1
89965Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID402801.1