Strain identifier
BacDive ID: 131489
Type strain: 
Species: Modestobacter muralis
Strain Designation: MDVD1
Strain history: CECT 8845 <-- M. E. Trujillo, Univ. Salamanca, Spain; MDVD1.
NCBI tax ID(s): 1608614 (species)
version 9.1 (current version)
General
@ref: 22716
BacDive-ID: 131489
DSM-Number: 100205
keywords: genome sequence, 16S sequence, Bacteria, spore-forming
description: Modestobacter muralis MDVD1 is a spore-forming bacterium that was isolated from deteriorated sandstone from the surface of a historical building.
NCBI tax id
- NCBI tax id: 1608614
- Matching level: species
strain history
| @ref | history |
|---|---|
| 22716 | <- M. E. Trujillo, Universidad de Salamanca, Spain; MDVD1 |
| 67770 | CECT 8845 <-- M. E. Trujillo, Univ. Salamanca, Spain; MDVD1. |
doi: 10.13145/bacdive131489.20240916.9.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/actinomycetota
- domain: Bacteria
- phylum: Actinomycetota
- class: Actinomycetes
- order: Geodermatophilales
- family: Geodermatophilaceae
- genus: Modestobacter
- species: Modestobacter muralis
- full scientific name: Modestobacter muralis Trujillo et al. 2015
@ref: 22716
domain: Bacteria
phylum: Actinobacteria
class: Actinobacteria
order: Actinomycetales
family: Geodermatophilaceae
genus: Modestobacter
species: Modestobacter muralis
full scientific name: Modestobacter muralis Trujillo et al. 2015
strain designation: MDVD1
type strain: yes
Morphology
multimedia
- @ref: 22716
- multimedia content: https://www.dsmz.de/microorganisms/photos/DSM_100205.jpg
- caption: Medium 553 28°C
- intellectual property rights: © Leibniz-Institut DSMZ
Culture and growth conditions
culture medium
| @ref | name | growth | link | composition |
|---|---|---|---|---|
| 22716 | GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) | yes | https://mediadive.dsmz.de/medium/65 | Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water |
| 22716 | GPHF-MEDIUM (DSMZ Medium 553) | yes | https://mediadive.dsmz.de/medium/553 | Name: GPHF-MEDIUM (DSMZ Medium 553) Composition: Agar 20.0 g/l Glucose 10.0 g/l Beef extract 5.0 g/l Yeast extract 5.0 g/l Casein peptone 5.0 g/l CaCl2 x 2 H2O 0.74 g/l Distilled water |
| 22716 | GEO-MEDIUM (DSMZ Medium 714) | yes | https://mediadive.dsmz.de/medium/714 | Name: GEO-MEDIUM (DSMZ Medium 714) Composition: Agar 15.0 g/l CaCO3 1.0 g/l Starch 1.0 g/l Glucose 1.0 g/l Yeast extract 1.0 g/l Distilled water |
culture temp
| @ref | growth | type | temperature |
|---|---|---|---|
| 22716 | positive | growth | 28 |
| 67770 | positive | growth | 28 |
Physiology and metabolism
spore formation
- @ref: 69481
- spore formation: yes
- confidence: 97
observation
- @ref: 67770
- observation: quinones: MK-9(H4)
Isolation, sampling and environmental information
isolation
| @ref | sample type | geographic location | country | origin.country | continent | latitude | longitude |
|---|---|---|---|---|---|---|---|
| 22716 | deteriorated sandstone from the surface of a historical building | Salamanca (40° 57' 45'' N; 5° 45' 00'' W) | Spain | ESP | Europe | 40.9625 | -5.75 |
| 67770 | Deteriorated sandstone scraped from the surface of a historic building | Salamanca | Spain | ESP | Europe |
isolation source categories
| Cat1 | Cat2 | Cat3 |
|---|---|---|
| #Engineered | #Built environment | |
| #Environmental | #Terrestrial | #Geologic |
Safety information
risk assessment
- @ref: 22716
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 22716
- description: Modestobacter sp. MDVD1 partial 16S rRNA gene, strain MDVD1
- accession: LN810545
- length: 1459
- database: nuccore
- NCBI tax ID: 1608614
Genome sequences
| @ref | description | accession | assembly level | database | NCBI tax ID |
|---|---|---|---|---|---|
| 66792 | Modestobacter muralis strain DSM 100205 | 1608614.3 | wgs | patric | 1608614 |
| 66792 | Modestobacter muralis strain DSM 100205 | 1608614.4 | wgs | patric | 1608614 |
| 67770 | Modestobacter muralis DSM 100205 | GCA_010686625 | contig | ncbi | 1608614 |
| 67770 | Modestobacter muralis DSM 100205 | GCA_010682105 | contig | ncbi | 1608614 |
GC content
- @ref: 22716
- GC-content: 71.5
- method: thermal denaturation, midpoint method (Tm)
Genome-based predictions
predictions
| @ref | trait | model | description | prediction | confidence | training_data |
|---|---|---|---|---|---|---|
| 69481 | spore-forming | spore-forming | Ability to form endo- or exospores | yes | 97 | no |
| 69480 | gram-positive | gram-positive | Positive reaction to Gram-staining | yes | 85.197 | no |
| 69480 | anaerobic | anaerobic | Ability to grow under anoxygenic conditions (including facultative anaerobes) | no | 95.937 | no |
| 69480 | spore-forming | spore-forming | Ability to form endo- or exospores | yes | 63.223 | no |
| 69480 | aerobic | aerobic | Ability to grow under oxygenic conditions (including facultative aerobes) | yes | 89.222 | no |
| 69480 | thermophilic | thermophile | Ability to grow at temperatures above or equal to 45°C | no | 93 | no |
| 69480 | flagellated | motile2+ | Ability to perform flagellated movement | no | 63 | no |
External links
@ref: 22716
culture collection no.: DSM 100205, CECT 8845, JCM 31704
straininfo link
- @ref: 89906
- straininfo: 404142
literature
- topic: Phylogeny
- Pubmed-ID: 25987397
- title: Modestobacter lapidis sp. nov. and Modestobacter muralis sp. nov., isolated from a deteriorated sandstone historic building in Salamanca, Spain.
- authors: Trujillo ME, Goodfellow M, Busarakam K, Riesco R
- journal: Antonie Van Leeuwenhoek
- DOI: 10.1007/s10482-015-0482-7
- year: 2015
- mesh: Actinobacteria/*classification/genetics/*isolation & purification/physiology, Bacterial Typing Techniques, Cluster Analysis, Cytosol/chemistry, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, *Environmental Microbiology, Fatty Acids/analysis, Molecular Sequence Data, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Spain
- topic2: Genetics
Reference
| @id | authors | title | doi/url | catalogue |
|---|---|---|---|---|
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | 10.1099/ijsem.0.004332 | |
| 22716 | Curators of the DSMZ | https://www.dsmz.de/collection/catalogue/details/culture/DSM-100205 | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 100205) | |
| 66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | https://diaspora-project.de/progress.html#genomes | |
| 67770 | Curators of the JCM | https://jcm.brc.riken.jp/en/ | ||
| 69480 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | https://diaspora-project.de/progress.html#genomes | |
| 69481 | Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch | Predictions based on the model GenomeNet Sporulation v. 1 | 10.21203/rs.3.rs-2527258/v1 | |
| 89906 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | StrainInfo: A central database for resolving microbial strain identifiers | 10.60712/SI-ID404142.1 |