Strain identifier
BacDive ID: 131390
Type strain: 
Species: Oceanobacillus damuensis
Strain Designation: PT-20
Strain history: <- Z. Lei, Sichuan Univ., College of Light Ind., Textile and Food Eng., Chengdu, China; PT-20 <- L. Yang
NCBI tax ID(s): 937928 (species)
version 8.1 (current version)
General
@ref: 22617
BacDive-ID: 131390
DSM-Number: 26901
keywords: genome sequence, 16S sequence, Bacteria, spore-forming, mesophilic, Gram-positive
description: Oceanobacillus damuensis PT-20 is a spore-forming, mesophilic, Gram-positive bacterium that was isolated from saline alkali soil.
NCBI tax id
- NCBI tax id: 937928
- Matching level: species
strain history
- @ref: 22617
- history: <- Z. Lei, Sichuan Univ., College of Light Ind., Textile and Food Eng., Chengdu, China; PT-20 <- L. Yang
doi: 10.13145/bacdive131390.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/bacillota
- domain: Bacteria
- phylum: Bacillota
- class: Bacilli
- order: Caryophanales
- family: Bacillaceae
- genus: Oceanobacillus
- species: Oceanobacillus damuensis
- full scientific name: Oceanobacillus damuensis Long et al. 2015
@ref: 22617
domain: Bacteria
phylum: Firmicutes
class: Bacilli
order: Bacillales
family: Bacillaceae
genus: Oceanobacillus
species: Oceanobacillus damuensis
full scientific name: Oceanobacillus damuensis Long et al. 2015
strain designation: PT-20
type strain: yes
Morphology
cell morphology
| @ref | gram stain | confidence | motility |
|---|---|---|---|
| 69480 | positive | 100 | |
| 69480 | 97.279 | yes |
Culture and growth conditions
culture medium
- @ref: 22617
- name: BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514)
- growth: yes
- link: https://mediadive.dsmz.de/medium/514
- composition: Name: BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514; with strain-specific modifications) Composition: NaCl 80.0 g/l MgCl2 5.9 g/l Bacto peptone 5.0 g/l Na2SO4 3.24 g/l CaCl2 1.8 g/l Yeast extract 1.0 g/l KCl 0.55 g/l NaHCO3 0.16 g/l Fe(III) citrate 0.1 g/l KBr 0.08 g/l SrCl2 0.034 g/l H3BO3 0.022 g/l Na2HPO4 0.008 g/l Na-silicate 0.004 g/l NaF 0.0024 g/l (NH4)NO3 0.0016 g/l Distilled water
culture temp
- @ref: 22617
- growth: positive
- type: growth
- temperature: 28
- range: mesophilic
Physiology and metabolism
spore formation
| @ref | spore formation | confidence |
|---|---|---|
| 69481 | yes | 100 |
| 69480 | yes | 100 |
Isolation, sampling and environmental information
isolation
- @ref: 22617
- sample type: saline alkali soil
- geographic location: Xinjiang Province, Shache County, Damu town
- country: China
- origin.country: CHN
- continent: Asia
isolation source categories
| Cat1 | Cat2 | Cat3 |
|---|---|---|
| #Environmental | #Terrestrial | #Soil |
| #Condition | #Alkaline | |
| #Condition | #Saline |
taxonmaps
- @ref: 69479
- File name: preview.99_108864.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_3;96_867;97_12822;98_61078;99_108864&stattab=map
- Last taxonomy: Oceanobacillus
- 16S sequence: HQ620704
- Sequence Identity:
- Total samples: 56
- soil counts: 19
- aquatic counts: 10
- animal counts: 19
- plant counts: 8
Safety information
risk assessment
- @ref: 22617
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 22617
- description: Oceanobacillus damuensis strain PT-20 16S ribosomal RNA gene, partial sequence
- accession: HQ620704
- length: 1455
- database: ena
- NCBI tax ID: 937928
Genome sequences
| @ref | description | accession | assembly level | database | NCBI tax ID |
|---|---|---|---|---|---|
| 66792 | Oceanobacillus damuensis PT-20 | GCA_001618145 | scaffold | ncbi | 937928 |
| 66792 | Oceanobacillus damuensis PT-20 | 2775506911 | draft | img | 937928 |
GC content
- @ref: 22617
- GC-content: 39.2
- method: high performance liquid chromatography (HPLC)
Genome-based predictions
predictions
| trait | prediction | confidence | training_data |
|---|---|---|---|
| spore-forming | yes | 100 | no |
| flagellated | yes | 90.259 | no |
| gram-positive | yes | 88.198 | no |
| anaerobic | no | 98.542 | no |
| aerobic | yes | 92.135 | no |
| halophile | yes | 89.381 | no |
| spore-forming | yes | 91.506 | no |
| glucose-util | yes | 87.413 | no |
| motile | yes | 90.12 | no |
| thermophile | no | 99.33 | yes |
| glucose-ferment | no | 84.562 | no |
External links
@ref: 22617
culture collection no.: DSM 26901, KCTC 33146
straininfo link
- @ref: 89823
- straininfo: 407555
literature
| topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
|---|---|---|---|---|---|---|---|---|
| Phylogeny | 26168852 | Oceanobacillus damuensis sp. nov. and Oceanobacillus rekensis sp. nov., isolated from saline alkali soil samples. | Long X, Ye R, Zhang S, Liu B, Zhang Y, Zeng Z, Tian Y | Antonie Van Leeuwenhoek | 10.1007/s10482-015-0529-9 | 2015 | Aerobiosis, Alkalies/analysis, Bacillaceae/*classification/genetics/*isolation & purification/physiology, Bacterial Typing Techniques, Base Composition, China, Cluster Analysis, Cytosol/chemistry, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Fatty Acids/analysis, Hydrogen-Ion Concentration, Locomotion, Microscopy, Electron, Transmission, Molecular Sequence Data, Nucleic Acid Hybridization, Phospholipids/analysis, Phylogeny, Quinones/analysis, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Soil/chemistry, *Soil Microbiology, Temperature | Genetics |
| Phylogeny | 27059624 | Oceanobacillus endoradicis sp. nov., an endophytic bacterial species isolated from the root of Paris polyphylla Smith var. yunnanensis. | Yang LL, Tang SK, Chu X, Jiang Z, Xu LH, Zhi XY | Antonie Van Leeuwenhoek | 10.1007/s10482-016-0695-4 | 2016 | Bacillaceae/*classification/genetics/*isolation & purification/metabolism, China, Diaminopimelic Acid/metabolism, Fatty Acids/metabolism, Melanthiaceae/*microbiology, Nucleic Acid Hybridization, Peptidoglycan/metabolism, Phenotype, Phospholipids/metabolism, Phylogeny, Plant Roots/*microbiology, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Sodium Chloride/metabolism, Soil Microbiology, Vitamin K 2/analogs & derivatives/metabolism | Metabolism |
Reference
| @id | authors | title | doi/url | catalogue |
|---|---|---|---|---|
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | 10.1099/ijsem.0.004332 | |
| 22617 | Curators of the DSMZ | https://www.dsmz.de/collection/catalogue/details/culture/DSM-26901 | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 26901) | |
| 66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | https://diaspora-project.de/progress.html#genomes | |
| 69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | MicrobeAtlas 1.0 beta | https://microbeatlas.org/ | |
| 69480 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | https://diaspora-project.de/progress.html#genomes | |
| 69481 | Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch | Predictions based on the model GenomeNet Sporulation v. 1 | 10.21203/rs.3.rs-2527258/v1 | |
| 89823 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | StrainInfo: A central database for resolving microbial strain identifiers | 10.60712/SI-ID407555.1 |