Strain identifier

BacDive ID: 13134

Type strain: Yes

Species: Pseudomonas mandelii

Strain history: CIP <- 1997, D. Izard, Lille Univ., Lille, France: strain CFML 95-303

NCBI tax ID(s): 1215107 (strain), 75612 (species)

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General

@ref: 7299

BacDive-ID: 13134

DSM-Number: 17967

keywords: genome sequence, 16S sequence, Bacteria, aerobe, mesophilic, Gram-negative, motile, rod-shaped

description: Pseudomonas mandelii DSM 17967 is an aerobe, mesophilic, Gram-negative bacterium that was isolated from mineral water.

NCBI tax id

NCBI tax idMatching level
1215107strain
75612species

strain history

@refhistory
7299<- CIP <- D. Izard, Univ. Lille; CFML 95-303
67770IAM 15164 <-- CIP 105273 <-- D. Izard CFML 95-303.
120774CIP <- 1997, D. Izard, Lille Univ., Lille, France: strain CFML 95-303

doi: 10.13145/bacdive13134.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Pseudomonadales
  • family: Pseudomonadaceae
  • genus: Pseudomonas
  • species: Pseudomonas mandelii
  • full scientific name: Pseudomonas mandelii Verhille et al. 1999

@ref: 7299

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Pseudomonadales

family: Pseudomonadaceae

genus: Pseudomonas

species: Pseudomonas mandelii

full scientific name: Pseudomonas mandelii Verhille et al. 1999

type strain: yes

Morphology

cell morphology

  • @ref: 120774
  • gram stain: negative
  • cell shape: rod-shaped
  • motility: yes

pigmentation

  • @ref: 120774
  • production: no
  • name: Pyocyanin

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
7299TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535)yeshttps://mediadive.dsmz.de/medium/535Name: TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Distilled water
39248MEDIUM 3 - Columbia agaryesColumbia agar (39.000 g);distilled water (1000.000 ml)
120774CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72
120774CIP Medium 3yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=3

culture temp

@refgrowthtypetemperaturerange
7299positivegrowth30mesophilic
39248positivegrowth30mesophilic
55322positivegrowth30mesophilic
67770positivegrowth30mesophilic
120774positivegrowth5-30
120774nogrowth37mesophilic
120774nogrowth41thermophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
55322aerobe
120774obligate aerobe

halophily

@refsaltgrowthtested relationconcentration
120774NaClpositivegrowth0-4 %
120774NaClnogrowth6 %
120774NaClnogrowth8 %
120774NaClnogrowth10 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6836925115malate+assimilation
6836917128adipate-assimilation
6836927689decanoate+assimilation
6836924265gluconate+assimilation
6836917306maltose-assimilation
6836959640N-acetylglucosamine+assimilation
6836916899D-mannitol+assimilation
6836916024D-mannose+assimilation
6836930849L-arabinose+assimilation
6836917634D-glucose+assimilation
683695291gelatin-hydrolysis
683694853esculin-hydrolysis
6836916199urea-hydrolysis
6836929016arginine+hydrolysis
6836917634D-glucose-fermentation
6836927897tryptophan-energy source
6836917632nitrate+reduction
12077416947citrate+carbon source
1207744853esculin-hydrolysis
12077417632nitrate+reduction
12077416301nitrite-reduction
12077417632nitrate+respiration

antibiotic resistance

  • @ref: 120774
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: no
  • is resistant: yes

metabolite production

@refChebi-IDmetaboliteproduction
6836935581indoleno
12077435581indoleno

metabolite tests

  • @ref: 68369
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: -

enzymes

@refvalueactivityec
68369cytochrome oxidase+1.9.3.1
68369gelatinase-
68369beta-glucosidase-3.2.1.21
68369urease-3.5.1.5
68369arginine dihydrolase+3.5.3.6
120774oxidase+
120774beta-galactosidase-3.2.1.23
120774alcohol dehydrogenase+1.1.1.1
120774gelatinase-
120774amylase-
120774DNase-
120774caseinase-3.4.21.50
120774catalase+1.11.1.6
120774tween esterase-
120774lecithinase-
120774lipase-
120774lysine decarboxylase-4.1.1.18
120774ornithine decarboxylase-4.1.1.17
120774tryptophan deaminase-
120774urease-3.5.1.5
68382alkaline phosphatase-3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase-3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
120774--++-+----++--------

API 20NE

@refNO3TRPGLU_ FermADH ArgUREESCGELPNPGGLU_ AssimARAMNEMANNAGMALGNTCAPADIMLTCITPACOX
7299+--+----+++++-++-++-+
7299+--+----+++++-++-++-+

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
120774+++++--------------+++----+----+++--+------++--+-+++-+++---++-+++--+---+++++++-++++++-+-+++++++++++

Isolation, sampling and environmental information

isolation

@refsample typecountryorigin.countrycontinentsampling dateisolation date
7299mineral waterFranceFRAEurope
55322Mineral waterFranceFRAEurope1995
67770Natural mineral water
120774Environment, Mineral waterFranceFRAEurope1995

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Aquatic

taxonmaps

  • @ref: 69479
  • File name: preview.99_555.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_23;96_61;97_89;98_98;99_555&stattab=map
  • Last taxonomy: Pseudomonas
  • 16S sequence: AF058286
  • Sequence Identity:
  • Total samples: 42314
  • soil counts: 16904
  • aquatic counts: 8731
  • animal counts: 11521
  • plant counts: 5158

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
72991Risk group (German classification)
1207741Risk group (French classification)

Sequence information

16S sequences

  • @ref: 7299
  • description: Pseudomonas mandelii 16S ribosomal RNA gene, complete sequence
  • accession: AF058286
  • length: 1518
  • database: ena
  • NCBI tax ID: 75612

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Pseudomonas mandelii NBRC 103147 strain NBRC 1031471215107.3wgspatric1215107
66792Pseudomonas mandelii strain DSM 1796775612.18wgspatric75612
66792Pseudomonas mandelii NBRC 1031472814123354draftimg1215107
67770Pseudomonas mandelii NBRC 103147GCA_002091695contigncbi1215107
67770Pseudomonas mandelii LMG 21607GCA_900106065chromosomencbi75612
67770Pseudomonas mandelii DSM 17967GCA_007858265contigncbi75612

GC content

  • @ref: 67770
  • GC-content: 57
  • method: high performance liquid chromatography (HPLC)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
motileyes91.799no
gram-positiveno98.15no
anaerobicno97.611yes
aerobicyes89.432yes
halophileno91.605no
spore-formingno94.311no
thermophileno99.908yes
glucose-utilyes94.937yes
flagellatedyes79.146no
glucose-fermentno89.638no

External links

@ref: 7299

culture collection no.: DSM 17967, ATCC 700871, CCUG 42058, CIP 105273, CCM 4842, CFML 95-303, JCM 21619, IAM 15164, NBRC 103147

straininfo link

  • @ref: 82342
  • straininfo: 44186

literature

  • topic: Phylogeny
  • Pubmed-ID: 10188278
  • title: Taxonomic study of bacteria isolated from natural mineral waters: proposal of Pseudomonas jessenii sp. nov. and Pseudomonas mandelii sp. nov.
  • authors: Verhille S, Baida N, Dabboussi F, Izard D, Leclerc H
  • journal: Syst Appl Microbiol
  • DOI: 10.1016/S0723-2020(99)80027-7
  • year: 1999
  • mesh: DNA, Bacterial/analysis, Nucleic Acid Hybridization, Phylogeny, Pseudomonas/*classification/genetics/physiology, RNA, Ribosomal, 16S/genetics, *Water Microbiology

Reference

@idauthorscataloguedoi/urltitle
7299Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 17967)https://www.dsmz.de/collection/catalogue/details/culture/DSM-17967
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
39248Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/17274
55322Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 42058)https://www.ccug.se/strain?id=42058
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68369Automatically annotated from API 20NE
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
82342Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID44186.1StrainInfo: A central database for resolving microbial strain identifiers
120774Curators of the CIPCollection of Institut Pasteur (CIP 105273)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20105273