Pseudomonas mandelii (DSM 17967, ATCC 700871, CCUG 42058)

Name: Pseudomonas mandelii (DSM 17967, ATCC 700871, CCUG 42058)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 13134

Type strain

Culture col. no. DSM 17967 ATCC 700871 CCUG 42058 CIP 105273 CCM 4842 4 more

NCBI tax ID(s) 1215107 75612

Special Collections DSMZ CCUG JCM CIP

Links

Pseudomonas mandelii DSM 17967 is an aerobe, mesophilic, Gram-negative prokaryote that was isolated from mineral water.

Gram-negative motile rod-shaped aerobe mesophilic genome sequence 16S sequence

Name and taxonomic classification

SI-ID 44186

ATCC 700871,CIP 105273,CCUG 42058,LMG 21607,CCM 4842,DSM 17967,JCM 21619,CFBP 4844,NBRC 103147,VTT E-052917,IAM 15164

@ref 20215

Last LPSN update 2025-12-16 09:50:01

Domain Pseudomonadati

Phylum Pseudomonadota

Class Gammaproteobacteria

Order Pseudomonadales

Family Pseudomonadaceae

Genus Pseudomonas

Species Pseudomonas mandelii

Full scientific name Pseudomonas mandelii Verhille et al. 1999

BacDive ID	Other strains from **Pseudomonas mandelii** (5)	Type strain
12967	P. mandelii PWD31, DSM 11566
147478	P. mandelii CCUG 33663
152655	P. mandelii CCUG 47301
153812	P. mandelii CCUG 51251
158066	P. mandelii 7HK4, DSM 107615

Morphology

Cell morphology

@ref	Gram stain	Cell shape	Confidence
120774	negative	rod-shaped
125438			90.369
125438	negative		98.5
125439	negative		95.9

Pigmentation

@ref	Production	Name
120774		Pyocyanin

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
7299	TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535)	Medium recipe at MediaDive	Name: TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Distilled water
39248	MEDIUM 3 - Columbia agar		Columbia agar (39.000 g);distilled water (1000.000 ml)
120774	CIP Medium 72	Medium recipe at CIP
120774	CIP Medium 3	Medium recipe at CIP

Culture temp

@ref	Growth	Type	Temperature (°C)	Range
7299	positive	growth	30	mesophilic
39248	positive	growth	30	mesophilic
55322	positive	growth	30	mesophilic
67770	positive	growth	30	mesophilic
120774	positive	growth	5-30
120774	negative	growth	37
120774	negative	growth	41

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
55322	aerobe
120774	obligate aerobe
125438	aerobe	91.932
125439	obligate aerobe	97.9

Spore formation

@ref	Spore formation	Confidence
125439		98.2

Halophily

@ref	Salt	Growth	Tested relation	Concentration
120774	NaCl	positive	growth	0-4 %
120774	NaCl		growth	6 %
120774	NaCl		growth	8 %
120774	NaCl		growth	10 %

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
68369	17128 ChEBI	adipate	-	assimilation	from API 20NE
68369	29016 ChEBI	arginine	+	hydrolysis	from API 20NE
120774	16947 ChEBI	citrate	+	carbon source
68369	17634 ChEBI	D-glucose	+	assimilation	from API 20NE
68369	17634 ChEBI	D-glucose	-	fermentation	from API 20NE
68369	16899 ChEBI	D-mannitol	+	assimilation	from API 20NE
68369	16024 ChEBI	D-mannose	+	assimilation	from API 20NE
68369	27689 ChEBI	decanoate	+	assimilation	from API 20NE
120774	4853 ChEBI	esculin	-	hydrolysis
68369	4853 ChEBI	esculin	-	hydrolysis	from API 20NE
68369	5291 ChEBI	gelatin	-	hydrolysis	from API 20NE
68369	24265 ChEBI	gluconate	+	assimilation	from API 20NE
68369	30849 ChEBI	L-arabinose	+	assimilation	from API 20NE
68369	25115 ChEBI	malate	+	assimilation	from API 20NE
68369	17306 ChEBI	maltose	-	assimilation	from API 20NE
68369	59640 ChEBI	N-acetylglucosamine	+	assimilation	from API 20NE
120774	17632 ChEBI	nitrate	+	reduction
120774	17632 ChEBI	nitrate	+	respiration
68369	17632 ChEBI	nitrate	+	reduction	from API 20NE
120774	16301 ChEBI	nitrite	-	reduction
68369	27897 ChEBI	tryptophan	-	energy source	from API 20NE
68369	16199 ChEBI	urea	-	hydrolysis	from API 20NE

Antibiotic resistance

@ref	Metabolite	Is antibiotic	Is sensitive	Is resistant
120774	0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)

Metabolite production

@ref	Chebi-ID	Metabolite	Production
68369	35581 ChEBI	indole		from API 20NE
120774	35581 ChEBI	indole

Metabolite tests

@ref	Chebi-ID	Metabolite	Indole test
68369	35581 ChEBI	indole	-	from API 20NE

Enzymes

@ref	Value	Activity	Ec
68382	acid phosphatase	+	3.1.3.2	from API zym
120774	alcohol dehydrogenase	+	1.1.1.1
68382	alkaline phosphatase	-	3.1.3.1	from API zym
68382	alpha-chymotrypsin	-	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68382	alpha-galactosidase	-	3.2.1.22	from API zym
68382	alpha-glucosidase	-	3.2.1.20	from API zym
68382	alpha-mannosidase	-	3.2.1.24	from API zym
120774	amylase	-
68369	arginine dihydrolase	+	3.5.3.6	from API 20NE
68382	beta-galactosidase	-	3.2.1.23	from API zym
120774	beta-galactosidase	-	3.2.1.23
68382	beta-glucosidase	-	3.2.1.21	from API zym
68369	beta-glucosidase	-	3.2.1.21	from API 20NE
68382	beta-glucuronidase	-	3.2.1.31	from API zym
120774	caseinase	-	3.4.21.50
120774	catalase	+	1.11.1.6
68382	cystine arylamidase	-	3.4.11.3	from API zym
68369	cytochrome oxidase	+	1.9.3.1	from API 20NE
120774	DNase	-
68382	esterase (C 4)	+		from API zym
68382	esterase lipase (C 8)	+		from API zym
120774	gelatinase	-
68369	gelatinase	-		from API 20NE
120774	lecithinase	-
68382	leucine arylamidase	+	3.4.11.1	from API zym
120774	lipase	-
68382	lipase (C 14)	-		from API zym
120774	lysine decarboxylase	-	4.1.1.18
68382	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API zym
68382	naphthol-AS-BI-phosphohydrolase	+		from API zym
120774	ornithine decarboxylase	-	4.1.1.17
120774	oxidase	+
68382	trypsin	-	3.4.21.4	from API zym
120774	tryptophan deaminase	-
120774	tween esterase	-
120774	urease	-	3.5.1.5
68369	urease	-	3.5.1.5	from API 20NE
68382	valine arylamidase	-		from API zym

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
120774	-	-	+	+	-	+	-	-	-	-	+	+	-	-	-	-	-	-	-	-

API 20NE

@ref	Reduction of nitratesNO3	TRP	GLU_ Ferm	ADH (Arg)	URE	ESC	GEL	PNPG	GLU_ Assim	ARA	MNE	MAN	NAG	MAL	GNT	CAP	ADI	MLT	CIT	PAC	OX
7299	+	-	-	+	-	-	-	-	+	+	+	+	+	-	+	+	-	+	+	-	+
7299	+	-	-	+	-	-	-	-	+	+	+	+	+	-	+	+	-	+	+	-	+

API biotype100

@ref	ControlQ	D-glucose as carbon sourceGLU	D-fructose as carbon sourceFRU	D-galactose as carbon sourceGAL	D-trehalose as carbon sourceTRE	D-mannose as carbon sourceMNE	L-sorbose as carbon sourceSBE	D-melibiose as carbon sourceMEL	sucrose as carbon sourceSAC	D-raffinose as carbon sourceRAF	maltotriose as carbon sourceMTE	maltose as carbon sourceMAL	lactose as carbon sourceLAC	lactulose as carbon sourceLTE	1-O-methyl-beta-galactopyranoside as carbon sourceMbGa	1-O-methyl-alpha-galactopyranoside as carbon sourceMaGa	D-cellobiose as carbon sourceCEL	gentiobiose as carbon sourceGEN	1-O-methyl-beta-D-glucopyranoside as carbon sourceMbGu	esculin as carbon sourceESC	D-ribose as carbon sourceRIB	L-arabinose as carbon sourceARA	D-xylose as carbon sourceXYL	palatinose as carbon sourcePLE	L-rhamnose as carbon sourceRHA	L-fucose as carbon sourceFUC	D-melezitose as carbon sourceMLZ	D-arabitol as carbon sourceDARL	L-arabitol as carbon sourceLARL	xylitol as carbon sourceXLT	dulcitol as carbon sourceDUL	D-tagatose as carbon sourceTAG	glycerol as carbon sourceGLY	myo-inositol as carbon sourceINO	D-mannitol as carbon sourceMAN	maltitol as carbon sourceMTL	D-turanose as carbon sourceTUR	D-sorbitol as carbon sourceSOR	adonitol as carbon sourceADO	hydroxyquinoline-beta-glucuronide as carbon sourceHBG	D-lyxose as carbon sourceLYX	i-erythritol as carbon sourceERY	1-O-methyl-alpha-D-glucoside as carbon sourceMDG	3-O-methyl-D-glucose as carbon source3MDG	D-saccharate as carbon sourceSAT	mucate as carbon sourceMUC	L-tartrate as carbon sourceLTAT	D-tartrate as carbon sourceDTAT	meso-tartrate as carbon sourceMTAT	D-malate as carbon sourceDMLT	L-malate as carbon sourceLMLT	cis-aconitate as carbon sourceCATE	trans-aconitate as carbon sourceTATE	tricarballylate as carbon sourceTTE	citrate as carbon sourceCIT	D-glucuronate as carbon sourceGRT	D-galacturonate as carbon sourceGAT	2-ketogluconate as carbon source2KG	5-ketogluconate as carbon source5KG	tryptophan as carbon sourceTRY	N-acetyl-D-glucosamine as carbon sourceNAG	D-gluconate as carbon sourceGNT	phenylacetate as carbon sourcePAC	protocatechuate as carbon sourcePAT	4-hydroxybenzoate as carbon sourcepOBE	quinate as carbon sourceQAT	gentisate as carbon sourceGTE	3-hydroxybenzoate as carbon sourcemOBE	benzoate as carbon sourceBAT	3-phenylpropionate as carbon sourcePPAT	m-coumarate as carbon sourceCMT	trigonelline as carbon sourceTGE	betaine as carbon sourceBET	putrescine as carbon sourcePCE	4-aminobutyrate as carbon sourceABT	histamine as carbon sourceHIN	DL-lactate as carbon sourceLAT	caprate as carbon sourceCAP	caprylate as carbon sourceCYT	L-histidine as carbon sourceHIS	succinate as carbon sourceSUC	fumarate as carbon sourceFUM	glutarate as carbon sourceGRE	DL-glycerate as carbon sourceGYT	5-aminovalerate as carbon sourceAVT	ethanolamine as carbon sourceETN	tryptamine as carbon sourceTTN	D-glucosamine as carbon sourceGLN	itaconate as carbon sourceITA	3-hydroxybutyrate as carbon source3OBU	L-aspartate as carbon sourceAPT	L-glutamate as carbon sourceGTT	L-proline as carbon sourcePRO	D-alanine as carbon sourceDALA	L-alanine as carbon sourceLALA	L-serine as carbon sourceSER	malonate as carbon sourceMNT	propionate as carbon sourcePROP	L-tyrosine as carbon sourceTYR	2-ketoglutarate as carbon source2KT
120774	not determinedn.d.	+	+	+	+	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	+	+	+	-	-	-	-	+	-	-	-	-	+	+	+	-	-	+	-	-	-	-	-	-	+	+	-	-	+	-	+	+	+	-	+	+	+	-	-	-	+	+	-	+	+	+	-	-	+	-	-	-	+	+	+	+	+	+	+	-	+	+	+	+	+	+	-	+	-	+	+	+	+	+	+	+	+	+	+	+

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2
#Environmental	#Aquatic

Isolation

@ref	Sample type	Sampling date	Country	Country ISO 3 Code	Continent	Isolation date
7299	mineral water		France	FRA	Europe
55322	Mineral water	1995	France	FRA	Europe
67770	Natural mineral water
120774	Environment, Mineral water		France	FRA	Europe	1995

Taxonmaps

69479

Global distribution of 16S sequence AF058286 (>99% sequence identity) for Pseudomonas from Microbeatlas

Aquatic Samples	8731
Soil Samples	16904
Animal Samples	11521
Plant Samples	5158
Total Samples	42314

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
7299	1	Risk group (German classification) According to TRBA 466
120774	1	Risk group (French classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
67770	IMG-taxon 2636416004 annotated assembly for Pseudomonas mandelii LMG 21607	chromosome	GCA_900106065	75612.10	2636416004	75612	89.31
67770	ASM209169v1 assembly for Pseudomonas mandelii NBRC 103147	contig	GCA_002091695	1215107.3	2814123354	1215107	58.32
67770	ASM785826v1 assembly for Pseudomonas mandelii DSM 17967	contig	GCA_007858265	75612.18	8117617677	75612	54.91

16S sequences

@ref	Description	Accession	Length	NCBI tax ID
7299	Pseudomonas mandelii 16S ribosomal RNA gene, complete sequence	AF058286	1518	75612
124043	Pseudomonas mandelii gene for 16S rRNA, partial sequence, strain: NBRC 103147.	AB681956	1464	75612
124043	Pseudomonas mandelii strain NBRC 103147 16S ribosomal RNA gene, partial sequence.	OQ618974	1272	75612
124043	Pseudomonas mandelii strain NBRC 103147 16S ribosomal RNA gene, partial sequence.	OQ619090	1309	75612
124043	Pseudomonas mandelii strain CIP 105273 16S ribosomal RNA gene, partial sequence.	OR660287	1109	75612

GC content

@ref	GC-content (mol%)	Method
67770	57	high performance liquid chromatography (HPLC)

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Pseudomonas mandelii NBRC 103147
NCBI: GCA_002091695

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	oxygen_tolerance	BacteriaNetⓘ	obligate aerobe	97.90	no
125439	gram_stain	BacteriaNetⓘ	negative	95.90	no
125439	motility	BacteriaNetⓘ	yes	78.60	no
125439	spore_formation	BacteriaNetⓘ	no	98.20	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Pseudomonas mandelii NBRC 103147 strain NBRC 103147
PATRIC: 1215107.3

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	no	98.50	no
125438	anaerobic	anaerobicⓘ	no	98.55	no
125438	spore-forming	spore-formingⓘ	no	81.84	no
125438	aerobic	aerobicⓘ	yes	91.93	no
125438	thermophilic	thermophileⓘ	no	98.00	yes
125438	flagellated	motile2+ⓘ	yes	90.37	no

Literature

Topic	Title	Authors	Journal	DOI	Year
	Whole-Genome Sequence and Classification of 11 Endophytic Bacteria from Poison Ivy (Toxicodendron radicans).	Tran PN, Tan NE, Lee YP, Gan HM, Polter SJ, Dailey LK, Hudson AO, Savka MA.	Genome Announc	10.1128/genomea.01319-15	2015
Enzymology	Diversity of Cultivable Microbes From Soil of the Fildes Peninsula, Antarctica, and Their Potential Application.	Cong B, Yin X, Deng A, Shen J, Tian Y, Wang S, Yang H.	Front Microbiol	10.3389/fmicb.2020.570836	2020
Phylogeny	Microbial diversity and soil physiochemical characteristic of higher altitude.	Kumar S, Suyal DC, Yadav A, Shouche Y, Goel R.	PLoS One	10.1371/journal.pone.0213844	2019
Phylogeny	Ubiquity and Diversity of Cold Adapted Denitrifying Bacteria Isolated From Diverse Antarctic Ecosystems.	Cabezas A, Azziz G, Bovio-Winkler P, Fuentes L, Braga L, Wenzel J, Sabaris S, Tarlera S, Etchebehere C.	Front Microbiol	10.3389/fmicb.2022.827228	2022
Metabolism	Assessment of toluene/biphenyl dioxygenase gene diversity in benzene-polluted soils: links between benzene biodegradation and genes similar to those encoding isopropylbenzene dioxygenases.	Witzig R, Junca H, Hecht HJ, Pieper DH.	Appl Environ Microbiol	10.1128/aem.72.5.3504-3514.2006	2006
	Culture-Dependent Bioprospecting of Bacterial Isolates From the Canadian High Arctic Displaying Antibacterial Activity.	Marcolefas E, Leung T, Okshevsky M, McKay G, Hignett E, Hamel J, Aguirre G, Blenner-Hassett O, Boyle B, Levesque RC, Nguyen D, Gruenheid S, Whyte L.	Front Microbiol	10.3389/fmicb.2019.01836	2019
Metabolism	Aerobic and oxygen-limited naphthalene-amended enrichments induced the dominance of Pseudomonas spp. from a groundwater bacterial biofilm.	Benedek T, Szentgyorgyi F, Szabo I, Farkas M, Duran R, Kriszt B, Tancsics A.	Appl Microbiol Biotechnol	10.1007/s00253-020-10668-y	2020
	Pseudomonas cucumis sp. nov., isolated from the rhizosphere of crop plants.	Liao K, Liu J, Gu YL, Wang C, Wei HL.	Int J Syst Evol Microbiol	10.1099/ijsem.0.006208	2023
Phylogeny	Taxonomic study of bacteria isolated from natural mineral waters: proposal of Pseudomonas jessenii sp. nov. and Pseudomonas mandelii sp. nov.	Verhille S, Baida N, Dabboussi F, Izard D, Leclerc H	Syst Appl Microbiol	10.1016/S0723-2020(99)80027-7	1999

References

#7299	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 17967
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#39248	; Curators of the CIP;
#55322	Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 42058
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#68369	Automatically annotated from API 20NE .
#68382	Automatically annotated from API zym .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#120774	Collection of Institut Pasteur ; Curators of the CIP; CIP 105273
#124043	Isabel Schober, Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Pseudomonas mandelii (DSM 17967, ATCC 700871, CCUG 42058)

Name and taxonomic classification

Morphology

Cell morphology

Pigmentation

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Spore formation

Halophily

Metabolite utilization

Antibiotic resistance

Metabolite production

Metabolite tests

Enzymes

API zym

API 20NE

API biotype100

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_900106065

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

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