Strain identifier
BacDive ID: 131131
Type strain: 
Species: Thermorudis peleae
Strain Designation: KI4
Strain history: <- C. King, Louisiana State Univ., Baton Rouge, USA; KI4
NCBI tax ID(s): 1382356 (species)
version 8.1 (current version)
General
@ref: 22423
BacDive-ID: 131131
DSM-Number: 27169
keywords: genome sequence, 16S sequence, Bacteria, thermophilic
description: Thermorudis peleae KI4 is a thermophilic bacterium that was isolated from geothermally heated biofilm on a steaming tumulus.
NCBI tax id
- NCBI tax id: 1382356
- Matching level: species
strain history
- @ref: 22423
- history: <- C. King, Louisiana State Univ., Baton Rouge, USA; KI4
doi: 10.13145/bacdive131131.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/thermomicrobiota
- domain: Bacteria
- phylum: Thermomicrobiota
- class: Thermomicrobia
- order: Thermomicrobiales
- family: Thermomicrobiaceae
- genus: Thermorudis
- species: Thermorudis peleae
- full scientific name: Thermorudis peleae King and King 2014
@ref: 22423
domain: Bacteria
phylum: Thermomicrobia
class: Thermomicrobia
order: Thermomicrobia, not assigned to order
family: Thermomicrobiaceae
genus: Thermorudis
species: Thermorudis peleae
full scientific name: Thermorudis peleae King and King 2014 emend. Houghton et al. 2015
strain designation: KI4
type strain: yes
Culture and growth conditions
culture medium
| @ref | name | growth | link | composition |
|---|---|---|---|---|
| 22423 | THERMOMICROBIUM ROSEUM MEDIUM (DSMZ Medium 592) | yes | https://mediadive.dsmz.de/medium/592 | Name: THERMOMICROBIUM ROSEUM MEDIUM (DSMZ Medium 592) Composition: Agar 20.0 g/l (NH4)2SO4 1.3 g/l Yeast extract 1.0 g/l Tryptone 1.0 g/l KH2PO4 0.28 g/l MgSO4 x 7 H2O 0.247 g/l CaCl2 x 2 H2O 0.074 g/l Na2B4O7 x 10 H2O 0.044 g/l FeCl3 x 6 H2O 0.019 g/l MnCl2 x 4 H2O 0.018 g/l ZnSO4 x 7 H2O 0.0022 g/l CuCl2 x H2O 0.0005 g/l VOSO4 x 2 H2O 0.0003 g/l Na2MoO4 x 2 H2O 0.0003 g/l Distilled water |
| 22423 | R2A MEDIUM (DSMZ Medium 830) | yes | https://mediadive.dsmz.de/medium/830 | Name: R2A MEDIUM (DSMZ Medium 830) Composition: Agar 15.0 g/l Casamino acids 0.5 g/l Starch 0.5 g/l Glucose 0.5 g/l Proteose peptone 0.5 g/l Yeast extract 0.5 g/l K2HPO4 0.3 g/l Na-pyruvate 0.3 g/l MgSO4 x 7 H2O 0.05 g/l Distilled water |
culture temp
- @ref: 22423
- growth: positive
- type: growth
- temperature: 65
- range: thermophilic
Isolation, sampling and environmental information
isolation
- @ref: 22423
- sample type: geothermally heated biofilm on a steaming tumulus
- geographic location: Hawaii, Kilauea Iki crater (19° 24' 51.1'' N 155° 14' 57.4'' W)
- country: USA
- origin.country: USA
- continent: North America
- latitude: 19.4142
- longitude: -155.249
isolation source categories
| Cat1 | Cat2 | Cat3 |
|---|---|---|
| #Environmental | #Biofilm | |
| #Environmental | #Terrestrial | #Geologic |
taxonmaps
- @ref: 69479
- File name: preview.99_7545.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_651;96_3521;97_4320;98_5531;99_7545&stattab=map
- Last taxonomy: Thermorudis
- 16S sequence: KF193530
- Sequence Identity:
- Total samples: 1187
- soil counts: 413
- aquatic counts: 208
- animal counts: 541
- plant counts: 25
Safety information
risk assessment
- @ref: 22423
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 22423
- description: Thermorudis peleae strain KI4 from USA 16S ribosomal RNA gene, partial sequence
- accession: KF193530
- length: 1245
- database: ena
- NCBI tax ID: 1382356
Genome sequences
- @ref: 66792
- description: Thermorudis peleae KI4
- accession: GCA_000744775
- assembly level: contig
- database: ncbi
- NCBI tax ID: 1382356
GC content
- @ref: 22423
- GC-content: 60.7
- method: high performance liquid chromatography (HPLC)
Genome-based predictions
predictions
| trait | prediction | confidence | training_data |
|---|---|---|---|
| motile | yes | 52.635 | no |
| flagellated | no | 94.095 | no |
| gram-positive | yes | 55.036 | no |
| anaerobic | no | 95.618 | no |
| aerobic | yes | 81.847 | no |
| halophile | no | 91.256 | no |
| spore-forming | no | 86.263 | no |
| glucose-util | yes | 88.8 | no |
| thermophile | yes | 100 | yes |
| glucose-ferment | no | 80.39 | no |
External links
@ref: 22423
culture collection no.: DSM 27169, ATCC BAA 2536
straininfo link
- @ref: 89585
- straininfo: 406116
literature
| topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
|---|---|---|---|---|---|---|---|---|
| Phylogeny | 24814334 | Thermomicrobium carboxidum sp. nov., and Thermorudis peleae gen. nov., sp. nov., carbon monoxide-oxidizing bacteria isolated from geothermally heated biofilms. | King CE, King GM | Int J Syst Evol Microbiol | 10.1099/ijs.0.060327-0 | 2014 | Base Composition, *Biofilms, Carbon Monoxide/*metabolism, Chloroflexi/*classification/genetics/isolation & purification, DNA, Bacterial/genetics, Fatty Acids/chemistry, Glycolipids/chemistry, Hawaii, Hot Temperature, Molecular Sequence Data, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Volcanic Eruptions | Stress |
| Phylogeny | 26374291 | Thermorudis pharmacophila sp. nov., a novel member of the class Thermomicrobia isolated from geothermal soil, and emended descriptions of Thermomicrobium roseum, Thermomicrobium carboxidum, Thermorudis peleae and Sphaerobacter thermophilus. | Houghton KM, Morgan XC, Lagutin K, MacKenzie AD, Vyssotskii M, Mitchell KA, McDonald IR, Morgan HW, Power JF, Moreau JW, Hanssen E, Stott MB | Int J Syst Evol Microbiol | 10.1099/ijsem.0.000598 | 2015 | Bacterial Typing Techniques, Base Composition, Chloroflexi/*classification/genetics/isolation & purification, DNA, Bacterial/genetics, Fatty Acids/chemistry, Hot Springs, Hot Temperature, Molecular Sequence Data, New Zealand, Nucleic Acid Hybridization, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Soil Microbiology, Vitamin K 2/analogs & derivatives/chemistry | Stress |
Reference
| @id | authors | title | doi/url | catalogue |
|---|---|---|---|---|
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | 10.1099/ijsem.0.004332 | |
| 22423 | Curators of the DSMZ | https://www.dsmz.de/collection/catalogue/details/culture/DSM-27169 | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 27169) | |
| 66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | https://diaspora-project.de/progress.html#genomes | |
| 69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | MicrobeAtlas 1.0 beta | https://microbeatlas.org/ | |
| 89585 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | StrainInfo: A central database for resolving microbial strain identifiers | 10.60712/SI-ID406116.1 |