Strain identifier

BacDive ID: 131033

Type strain: No

Species: Lactobacillus johnsonii

Strain Designation: MIII-1B.7-2

Strain history: <- T. Clavel, TU Munich, Freising-Weihenstephan, Germany <- M. W. Hornef, RWTH Aachen, Germany; MIII-1B.7-2

NCBI tax ID(s): 33959 (species)

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General

@ref: 22325

BacDive-ID: 131033

DSM-Number: 100219

keywords: genome sequence, 16S sequence, Bacteria, microaerophile, mesophilic

description: Lactobacillus johnsonii MIII-1B.7-2 is a microaerophile, mesophilic bacterium that was isolated from newborn SPF mouse .

NCBI tax id

  • NCBI tax id: 33959
  • Matching level: species

strain history

  • @ref: 22325
  • history: <- T. Clavel, TU Munich, Freising-Weihenstephan, Germany <- M. W. Hornef, RWTH Aachen, Germany; MIII-1B.7-2

doi: 10.13145/bacdive131033.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Lactobacillales
  • family: Lactobacillaceae
  • genus: Lactobacillus
  • species: Lactobacillus johnsonii
  • full scientific name: Lactobacillus johnsonii Fujisawa et al. 1992

@ref: 22325

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Lactobacillales

family: Lactobacillaceae

genus: Lactobacillus

species: Lactobacillus johnsonii

full scientific name: Lactobacillus johnsonii Fujisawa et al. 1992

strain designation: MIII-1B.7-2

type strain: no

Morphology

colony morphology

  • @ref: 22325
  • incubation period: 1-2 days

Culture and growth conditions

culture medium

  • @ref: 22325
  • name: MRS MEDIUM (DSMZ Medium 11)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/11
  • composition: Name: MRS MEDIUM (DSMZ Medium 11) Composition: Glucose 20.0 g/l Casein peptone 10.0 g/l Meat extract 10.0 g/l Na-acetate 5.0 g/l Yeast extract 5.0 g/l (NH4)3 citrate 2.0 g/l K2HPO4 2.0 g/l Tween 80 1.0 g/l MgSO4 x 7 H2O 0.2 g/l MnSO4 x H2O 0.05 g/l Distilled water

culture temp

  • @ref: 22325
  • growth: positive
  • type: growth
  • temperature: 37
  • range: mesophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 22325
  • oxygen tolerance: microaerophile

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
68371Potassium 5-ketogluconate-builds acid from
68371Potassium 2-ketogluconate-builds acid from
68371gluconate-builds acid from24265
68371L-arabitol-builds acid from18403
68371D-arabitol-builds acid from18333
68371L-fucose-builds acid from18287
68371D-fucose-builds acid from28847
68371D-tagatose-builds acid from16443
68371D-lyxose-builds acid from62318
68371turanose-builds acid from32528
68371xylitol-builds acid from17151
68371glycogen-builds acid from28087
68371raffinose-builds acid from16634
68371melezitose-builds acid from6731
68371inulin-builds acid from15443
68371sucrose+builds acid from17992
68371melibiose-builds acid from28053
68371lactose+builds acid from17716
68371maltose+builds acid from17306
68371cellobiose+builds acid from17057
68371salicin-builds acid from17814
68371esculin+builds acid from4853
68371arbutin-builds acid from18305
68371amygdalin-builds acid from27613
68371N-acetylglucosamine+builds acid from59640
68371methyl alpha-D-glucopyranoside-builds acid from320061
68371methyl alpha-D-mannoside-builds acid from43943
68371D-sorbitol-builds acid from17924
68371D-mannitol-builds acid from16899
68371myo-inositol-builds acid from17268
68371galactitol-builds acid from16813
68371L-rhamnose-builds acid from62345
68371L-sorbose-builds acid from17266
68371D-fructose+builds acid from15824
68371D-glucose+builds acid from17634
68371methyl beta-D-xylopyranoside-builds acid from74863
68371ribitol-builds acid from15963
68371L-xylose-builds acid from65328
68371D-xylose-builds acid from65327
68371D-ribose-builds acid from16988
68371L-arabinose-builds acid from30849
68371D-arabinose-builds acid from17108
68371erythritol-builds acid from17113
68371glycerol-builds acid from17754

API 50CHac

@refQGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
22325----------+/-+++/---------+--+-+++-++/----+/---+/-----------

Isolation, sampling and environmental information

isolation

  • @ref: 22325
  • sample type: newborn SPF mouse (6-day-old)
  • geographic location: Freiburg
  • country: Germany
  • origin.country: DEU
  • continent: Europe

isolation source categories

  • Cat1: #Host
  • Cat2: #Mammals
  • Cat3: #Muridae (Mouse/Rat)

taxonmaps

  • @ref: 69479
  • File name: preview.99_840.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_46;96_506;97_581;98_668;99_840&stattab=map
  • Last taxonomy: Lactobacillus
  • 16S sequence: KU196088
  • Sequence Identity:
  • Total samples: 185237
  • soil counts: 4782
  • aquatic counts: 5652
  • animal counts: 173268
  • plant counts: 1535

Safety information

risk assessment

  • @ref: 22325
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 22325
  • description: Lactobacillus johnsonii strain DSM 100219 16S ribosomal RNA gene, partial sequence
  • accession: KU196088
  • length: 1473
  • database: ena
  • NCBI tax ID: 33959

Genome sequences

  • @ref: 66792
  • description: Lactobacillus johnsonii DSM 100219
  • accession: GCA_024622485
  • assembly level: contig
  • database: ncbi
  • NCBI tax ID: 33959

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
flagellatedno98.007no
gram-positiveyes96.023no
anaerobicno73.165yes
aerobicno97.38yes
halophileyes88.555no
spore-formingno95.053no
motileno96.246no
glucose-fermentyes91.526no
thermophileno99.745yes
glucose-utilyes92.134no

External links

@ref: 22325

culture collection no.: DSM 100219

straininfo link

  • @ref: 89503
  • straininfo: 400606

Reference

@idauthorstitledoi/urlcatalogue
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
22325Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-100219Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 100219)
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
68371Automatically annotated from API 50CH acid
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.MicrobeAtlas 1.0 betahttps://microbeatlas.org/
89503Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID400606.1