Strain identifier

BacDive ID: 131025

Type strain: Yes

Species: Streptomyces lonarensis

Strain history: <- L. Sunil Kumar, National Chemical Laboratory, Pune, India; NCL 716 <- M.C. Srinivasan and M. Rele

NCBI tax ID(s): 700599 (species)

« back to strain detail view
For citation purpose refer to the digital object identifier (doi) of the current version.
Archive
version 8.1 (current version):
version 8:
version 7.1:
version 7:
version 6:
version 5:
version 4.1:
version 4:
version 3:
version 2.1:
version 2:
version 1:
version 8.1 (current version)

General

@ref: 22317

BacDive-ID: 131025

DSM-Number: 42084

keywords: genome sequence, 16S sequence, Bacteria, mesophilic

description: Streptomyces lonarensis DSM 42084 is a mesophilic bacterium that builds an aerial mycelium and was isolated from soil from an alkaline salt water meteorite lake.

NCBI tax id

  • NCBI tax id: 700599
  • Matching level: species

strain history

  • @ref: 22317
  • history: <- L. Sunil Kumar, National Chemical Laboratory, Pune, India; NCL 716 <- M.C. Srinivasan and M. Rele

doi: 10.13145/bacdive131025.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Kitasatosporales
  • family: Streptomycetaceae
  • genus: Streptomyces
  • species: Streptomyces lonarensis
  • full scientific name: Streptomyces lonarensis Sharma et al. 2016

@ref: 22317

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Streptomycetaceae

genus: Streptomyces

species: Streptomyces lonarensis

full scientific name: Streptomyces lonarensis Sharma et al. 2016

type strain: yes

Morphology

colony morphology

@refcolony colormedium used
69477ColourlessISP 2
69477ColourlessISP 4
69477ColourlessISP 6
69477ColourlessISP 7
69477Ivory (1014)suter with tyrosine
69477Light ivory (1015)ISP 3
69477Light ivory (1015)ISP 5
69477Light ivory (1015)suter without tyrosine

multicellular morphology

@refforms multicellular complexcomplex namemedium namecomplex color
69477noAerial myceliumISP 2
69477noAerial myceliumISP 3
69477yesAerial myceliumISP 4Oyster white (1013)
69477yesAerial myceliumISP 5Traffic white (9016)
69477yesAerial myceliumISP 6Oyster white (1013)
69477yesAerial myceliumISP 7Signal white (9003)
69477noAerial myceliumsuter with tyrosine
69477noAerial myceliumsuter without tyrosine

pigmentation

@refproductionnamecolor
69477yesMelanin
69477yessoluble pigmentIvory (1014), sand yellow (1002), ocher brown (8001)

Culture and growth conditions

culture medium

  • @ref: 22317
  • name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/65
  • composition: Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water

culture temp

  • @ref: 22317
  • growth: positive
  • type: growth
  • temperature: 28
  • range: mesophilic

Physiology and metabolism

halophily

  • @ref: 69477
  • salt: NaCl
  • growth: positive
  • tested relation: growth
  • concentration: 0-5 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6947722599arabinose+growth
6947762968cellulose-growth
6947728757fructose+growth
6947717234glucose+growth
6947717268inositol+/-growth
6947737684mannose+growth
6947716634raffinose-growth
6947726546rhamnose+/-growth
6947717992sucrose+/-growth
6947718222xylose+growth
6837917632nitrate-reduction
683794853esculin-hydrolysis
6837916199urea-hydrolysis
683795291gelatin+hydrolysis
6837917634D-glucose-fermentation
6837916988D-ribose-fermentation
6837965327D-xylose-fermentation
6837916899D-mannitol-fermentation
6837917306maltose-fermentation
6837917716lactose-fermentation
6837917992sucrose-fermentation
6837928087glycogen-fermentation

enzymes

@refvalueactivityec
68379gelatinase+
68379urease-3.5.1.5
68379beta-glucosidase-3.2.1.21
68379N-acetyl-beta-glucosaminidase-3.2.1.52
68379alpha-glucosidase-3.2.1.20
68379beta-galactosidase-3.2.1.23
68379beta-glucuronidase-3.2.1.31
68379alkaline phosphatase+3.1.3.1
68379pyrrolidonyl arylamidase-3.4.19.3
68379pyrazinamidase-3.5.1.B15
68382alkaline phosphatase+3.1.3.1
68382leucine arylamidase+3.4.11.1
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382alpha-galactosidase-3.2.1.22
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase+3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase+3.2.1.52
68382alpha-fucosidase-3.2.1.51

API coryne

@refNITPYZPYRAPALbeta GURbeta GALalpha GLUbeta NAGESCUREGELGLURIBXYLMANMALLACSACGLYG
69477---+------+--------

API zym

@refAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
69477++/-+/-+/-++/-+/---++/--+/--+-++/--

Isolation, sampling and environmental information

isolation

  • @ref: 22317
  • sample type: soil from an alkaline salt water meteorite lake
  • geographic location: Maharashtra State, Buldhana district, Lonar Lake (19° 58' N 76° 31' E)
  • country: India
  • origin.country: IND
  • continent: Asia
  • latitude: 19.9667
  • longitude: 76.5167

isolation source categories

Cat1Cat2Cat3
#Environmental#Aquatic#Lake (large)
#Environmental#Terrestrial#Soil
#Condition#Alkaline
#Condition#Saline

Safety information

risk assessment

  • @ref: 22317
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 22317
  • description: Streptomyces lonarensis strain NCL 716 16S ribosomal RNA gene, partial sequence
  • accession: FJ919811
  • length: 1527
  • database: ena
  • NCBI tax ID: 700599

Genome sequences

  • @ref: 66792
  • description: Streptomyces lonarensis strain NCL716
  • accession: 700599.4
  • assembly level: wgs
  • database: patric
  • NCBI tax ID: 700599

GC content

  • @ref: 22317
  • GC-content: 71.4
  • method: high performance liquid chromatography (HPLC)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
gram-positiveyes90.876no
anaerobicno98.788no
halophileno71.745no
spore-formingyes88.952no
glucose-utilyes91.034yes
flagellatedno97.048no
thermophileno92.649no
motileno91.114no
aerobicyes89.233no
glucose-fermentno87.938no

External links

@ref: 22317

culture collection no.: DSM 42084, KCTC 39684, MTCC 11708, NCL 716

straininfo link

  • @ref: 89496
  • straininfo: 402056

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny26597560Streptomyces lonarensis sp. nov., isolated from Lonar Lake, a meteorite salt water lake in India.Sharma TK, Mawlankar R, Sonalkar VV, Shinde VK, Zhan J, Li WJ, Rele MV, Dastager SG, Kumar LSAntonie Van Leeuwenhoek10.1007/s10482-015-0626-92015Base Composition, DNA, Bacterial/genetics, DNA, Ribosomal/genetics, Fatty Acids/chemistry/metabolism, India, Lakes/*analysis, Molecular Sequence Data, Phylogeny, RNA, Ribosomal, 16S/genetics, Sodium Chloride/analysis/metabolism, *Soil Microbiology, Streptomyces/classification/genetics/*isolation & purification/metabolismMetabolism
Phylogeny31693473Streptomyces otsuchiensis sp. nov., a biosurfactant-producing actinobacterium isolated from marine sediment.Terahara T, Naemura T, Nampo Y, Kobayashi T, Imada C, Hamada M, Tamura TInt J Syst Evol Microbiol10.1099/ijsem.0.0036382019Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Diaminopimelic Acid/chemistry, Fatty Acids/chemistry, Geologic Sediments/*microbiology, Japan, Peptidoglycan/chemistry, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Seawater/*microbiology, Sequence Analysis, DNA, Streptomyces/*classification/isolation & purification, Vitamin K 2/analogs & derivatives/chemistryTranscriptome
Genetics32763929Draft Genome Sequence of Streptomyces sp. Strain ventii, Isolated from a Microbial Mat near Hydrothermal Vents within the Axial Seamount in the Pacific Ocean, and Resequencing of the Type Strains Streptomyces lonarensis NCL 716 and Streptomyces bohaiensis 11A07.Loughran RM, Mitchell EA, Vining OB, Gallegos DA, Deadmond MC, Wasson BJ, Pfannmuller KM, Paddock BE, Koyack MJ, Oline DK, Ushijima B, Saw JH, McPhail KL, Videau PMicrobiol Resour Announc10.1128/MRA.00607-202020Phylogeny
Phylogeny36401660Streptomyces spiramenti sp. nov., isolated from a deep-sea microbial mat.Loughran RM, Diefendorf CM, Reill-VanSise JR, Mitchell EA, Vining OB, Gallegos DA, Miller G, Koyack MJ, Oline DK, Rivers OS, Ushijima B, Saw JH, Gaylor MO, McPhail KL, Videau PArch Microbiol10.1007/s00203-022-03326-62022Phylogeny, RNA, Ribosomal, 16S/genetics, DNA, Bacterial/genetics, Base Composition, *Streptomyces

Reference

@idauthorstitledoi/urlcatalogue
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
22317Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-42084Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 42084)
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
68379Automatically annotated from API Coryne
68382Automatically annotated from API zym
69477Wink, J.Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweighttps://cdn.dsmz.de/wink/DSM%2042084.pdf
89496Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID402056.1