Strain identifier

BacDive ID: 130962

Type strain: No

Species: Ligilactobacillus murinus

Strain Designation: M5-8a

Strain history: <- T. Clavel, TU Munich, Freising-Weihenstephan, Germany <- M. W. Hornef, RWTH Aachen, Germany; M5-8a

NCBI tax ID(s): 1622 (species)

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General

@ref: 22254

BacDive-ID: 130962

DSM-Number: 100193

keywords: genome sequence, 16S sequence, Bacteria, microaerophile

description: Ligilactobacillus murinus M5-8a is a microaerophile bacterium that was isolated from newborn SPF mouse .

NCBI tax id

  • NCBI tax id: 1622
  • Matching level: species

strain history

  • @ref: 22254
  • history: <- T. Clavel, TU Munich, Freising-Weihenstephan, Germany <- M. W. Hornef, RWTH Aachen, Germany; M5-8a

doi: 10.13145/bacdive130962.20240916.9.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Lactobacillales
  • family: Lactobacillaceae
  • genus: Ligilactobacillus
  • species: Ligilactobacillus murinus
  • full scientific name: Ligilactobacillus murinus (Hemme et al. 1982) Zheng et al. 2020
  • synonyms

    • @ref: 20215
    • synonym: Lactobacillus murinus

@ref: 22254

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Lactobacillales

family: Lactobacillaceae

genus: Ligilactobacillus

species: Ligilactobacillus murinus

full scientific name: Ligilactobacillus murinus (Hemme et al. 1982) Zheng et al. 2020

strain designation: M5-8a

type strain: no

Morphology

cell morphology

  • @ref: 69480
  • gram stain: positive
  • confidence: 94.095

colony morphology

  • @ref: 22254
  • incubation period: 2-3 days

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
22254MRS MEDIUM (DSMZ Medium 11)yeshttps://mediadive.dsmz.de/medium/11Name: MRS MEDIUM (DSMZ Medium 11) Composition: Glucose 20.0 g/l Casein peptone 10.0 g/l Meat extract 10.0 g/l Na-acetate 5.0 g/l Yeast extract 5.0 g/l (NH4)3 citrate 2.0 g/l K2HPO4 2.0 g/l Tween 80 1.0 g/l MgSO4 x 7 H2O 0.2 g/l MnSO4 x H2O 0.05 g/l Distilled water
22254LB (Luria-Bertani) MEDIUM (DSMZ Medium 381)yeshttps://mediadive.dsmz.de/medium/381Name: LB (Luria-Bertani) MEDIUM (DSMZ Medium 381) Composition: Agar 20.0 g/l NaCl 10.0 g/l Tryptone 10.0 g/l Yeast extract 5.0 g/l Distilled water

culture temp

  • @ref: 22254
  • growth: positive
  • type: growth
  • temperature: 37

Physiology and metabolism

oxygen tolerance

  • @ref: 22254
  • oxygen tolerance: microaerophile

Isolation, sampling and environmental information

isolation

  • @ref: 22254
  • sample type: newborn SPF mouse (6-day-old)
  • geographic location: Freiburg
  • country: Germany
  • origin.country: DEU
  • continent: Europe

isolation source categories

  • Cat1: #Host
  • Cat2: #Mammals
  • Cat3: #Muridae (Mouse/Rat)

taxonmaps

  • @ref: 69479
  • File name: preview.99_1521.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_45;96_871;97_1018;98_1201;99_1521&stattab=map
  • Last taxonomy: Ligilactobacillus
  • 16S sequence: KU196090
  • Sequence Identity:
  • Total samples: 39087
  • soil counts: 571
  • aquatic counts: 383
  • animal counts: 37886
  • plant counts: 247

Safety information

risk assessment

  • @ref: 22254
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 22254
  • description: Ligilactobacillus murinus strain DSM 100193 16S ribosomal RNA gene, partial sequence
  • accession: KU196090
  • length: 1502
  • database: nuccore
  • NCBI tax ID: 1622

Genome sequences

  • @ref: 66792
  • description: Ligilactobacillus murinus DSM 100193
  • accession: GCA_024622385
  • assembly level: contig
  • database: ncbi
  • NCBI tax ID: 1622

Genome-based predictions

predictions

@reftraitmodeldescriptionpredictionconfidencetraining_data
69480gram-positivegram-positivePositive reaction to Gram-stainingyes94.095no
69480anaerobicanaerobicAbility to grow under anoxygenic conditions (including facultative anaerobes)no84.394no
69480aerobicaerobicAbility to grow under oxygenic conditions (including facultative aerobes)no97.199yes
69480spore-formingspore-formingAbility to form endo- or exosporesno86.014no
69480thermophilicthermophileAbility to grow at temperatures above or equal to 45°Cno95.5no
69480flagellatedmotile2+Ability to perform flagellated movementno88no

External links

@ref: 22254

culture collection no.: DSM 100193

straininfo link

  • @ref: 89439
  • straininfo: 404646

Reference

@idauthorstitledoi/urlcatalogue
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
22254Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-100193Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 100193)
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.MicrobeAtlas 1.0 betahttps://microbeatlas.org/
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
89439Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID404646.1