Strain identifier

BacDive ID: 130946

Type strain: No

Species: Enterococcus gallinarum

Strain Designation: JJM0609-2-2

Strain history: <- T. Clavel, TU Munich, Freising-Weihenstephan, Germany; JJM0609-2-2

NCBI tax ID(s): 1353 (species)

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General

@ref: 22238

BacDive-ID: 130946

DSM-Number: 100110

keywords: genome sequence, 16S sequence, Bacteria, microaerophile, mesophilic, human pathogen

description: Enterococcus gallinarum JJM0609-2-2 is a microaerophile, mesophilic human pathogen that was isolated from wild mouse.

NCBI tax id

  • NCBI tax id: 1353
  • Matching level: species

strain history

  • @ref: 22238
  • history: <- T. Clavel, TU Munich, Freising-Weihenstephan, Germany; JJM0609-2-2

doi: 10.13145/bacdive130946.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Lactobacillales
  • family: Enterococcaceae
  • genus: Enterococcus
  • species: Enterococcus gallinarum
  • full scientific name: Enterococcus gallinarum (Bridge and Sneath 1982) Collins et al. 1984

  • synonyms

    • @ref: 20215
    • synonym: Streptococcus gallinarum

@ref: 22238

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Lactobacillales

family: Enterococcaceae

genus: Enterococcus

species: Enterococcus gallinarum

full scientific name: Enterococcus gallinarum (Bridge and Sneath 1982) Collins et al. 1984

strain designation: JJM0609-2-2

type strain: no

Morphology

colony morphology

  • @ref: 22238
  • incubation period: 1-2 days

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
22238COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)yeshttps://mediadive.dsmz.de/medium/693Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base
22238TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)yeshttps://mediadive.dsmz.de/medium/92Name: TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Yeast extract 3.0 g/l Distilled water

culture temp

  • @ref: 22238
  • growth: positive
  • type: growth
  • temperature: 37
  • range: mesophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 22238
  • oxygen tolerance: microaerophile

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6838016199urea-hydrolysis
6838029016arginine+hydrolysis
6838016024D-mannose+fermentation
6838016634raffinose-fermentation
6838017632nitrate-reduction
6838027897tryptophan-energy source
6838129016arginine+hydrolysis
6838116988D-ribose+builds acid from
6838116899D-mannitol+builds acid from
6838130911sorbitol-builds acid from
6838117716lactose+builds acid from
6838127082trehalose+builds acid from
6838117992sucrose+builds acid from
6838130849L-arabinose+builds acid from
6838118333D-arabitol-builds acid from
6838140585alpha-cyclodextrin+builds acid from
68381606565hippurate+hydrolysis
6838128087glycogen-builds acid from
6838127941pullulan-builds acid from
6838117306maltose+builds acid from
6838128053melibiose-builds acid from
683816731melezitose-builds acid from
68381320055methyl beta-D-glucopyranoside+builds acid from
6838116443D-tagatose+builds acid from
6838116199urea-hydrolysis

metabolite production

@refChebi-IDmetaboliteproduction
6838035581indoleno
6838115688acetoinyes

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testindole test
6838115688acetoin+
6838035581indole-

enzymes

@refvalueactivityec
68381urease-3.5.1.5
68381beta-mannosidase+3.2.1.25
68381glycyl tryptophan arylamidase+
68381N-acetyl-beta-glucosaminidase+3.2.1.52
68381pyrrolidonyl arylamidase+3.4.19.3
68381beta-galactosidase+3.2.1.23
68381Alanyl-Phenylalanyl-Proline arylamidase-
68381beta-glucosidase+3.2.1.21
68381arginine dihydrolase+3.5.3.6
68380serine arylamidase-
68380glutamyl-glutamate arylamidase-
68380histidine arylamidase-
68380glycin arylamidase-
68380alanine arylamidase-3.4.11.2
68380tyrosine arylamidase-
68380pyrrolidonyl arylamidase+3.4.19.3
68380leucine arylamidase-3.4.11.1
68380leucyl glycin arylamidase-3.4.11.1
68380proline-arylamidase-3.4.11.5
68380L-arginine arylamidase+
68380alkaline phosphatase-3.1.3.1
68380tryptophan deaminase-4.1.99.1
68380N-acetyl-beta-glucosaminidase+3.2.1.52
68380beta-glucuronidase-3.2.1.31
68380alpha-arabinosidase-3.2.1.55
68380beta-glucosidase+3.2.1.21
68380alpha-glucosidase-3.2.1.20
68380alpha-galactosidase-3.2.1.22
68380arginine dihydrolase+3.5.3.6
68380urease-3.5.1.5

API rID32A

@refUREADH Argalpha GALbeta GALbeta GPalpha GLUbeta GLUalpha ARAbeta GURbeta NAGMNERAFNITINDPALArgAProALGAPheALeuAPyrATyrAAlaAGlyAHisAGGASerA
22238-+-+/-+/--+--++----+--+/--+------

API rID32STR

@refADH Argbeta GLUbeta GARbeta GURalpha GALPALRIBMANSORLACTRERAFSACLARADARLCDEXVPAPPAbeta GALPyrAbeta NAGGTAHIPGLYGPULMALMELMLZMbeta DGTAGbeta MANURE
22238+++---++-+++++-++-+++++--+--+++-
22238+++/-+-+++-++-++-++-+++++--+--+++-
22238+++/--+-++-++-++-++-+++++--+--+++-

Isolation, sampling and environmental information

isolation

  • @ref: 22238
  • sample type: wild mouse
  • geographic location: Freising
  • country: Germany
  • origin.country: DEU
  • continent: Europe

isolation source categories

  • Cat1: #Host
  • Cat2: #Mammals
  • Cat3: #Muridae (Mouse/Rat)

taxonmaps

  • @ref: 69479
  • File name: preview.99_759.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_13;96_19;97_19;98_605;99_759&stattab=map
  • Last taxonomy: Enterococcus
  • 16S sequence: KU196084
  • Sequence Identity:
  • Total samples: 18861
  • soil counts: 573
  • aquatic counts: 1598
  • animal counts: 15794
  • plant counts: 896

Safety information

risk assessment

  • @ref: 22238
  • pathogenicity human: yes
  • pathogenicity animal: yes
  • biosafety level: 2
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 22238
  • description: Enterococcus gallinarum strain DSM 100110 16S ribosomal RNA gene, partial sequence
  • accession: KU196084
  • length: 1498
  • database: ena
  • NCBI tax ID: 1353

Genome sequences

  • @ref: 66792
  • description: Enterococcus gallinarum DSM 100110
  • accession: GCA_024622625
  • assembly level: contig
  • database: ncbi
  • NCBI tax ID: 1353

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
flagellatedno80.709no
gram-positiveyes89.441no
anaerobicno94.132yes
aerobicno96.05yes
halophileyes64.37no
spore-formingno74.986no
motileyes54.208no
glucose-fermentyes87.128no
thermophileno99.524yes
glucose-utilyes86.741no

External links

@ref: 22238

culture collection no.: DSM 100110

straininfo link

  • @ref: 89423
  • straininfo: 397880

literature

  • topic: Pathogenicity
  • Pubmed-ID: 30692549
  • title: The flagellin of candidate live biotherapeutic Enterococcus gallinarum MRx0518 is a potent immunostimulant.
  • authors: Laute-Caly DL, Raftis EJ, Cowie P, Hennessy E, Holt A, Panzica DA, Sparre C, Minter B, Stroobach E, Mulder IE
  • journal: Sci Rep
  • DOI: 10.1038/s41598-018-36926-8
  • year: 2019
  • mesh: Antineoplastic Agents, Immunological/*immunology/pharmacology, Cell Line, Dendritic Cells/immunology, Enterococcus/genetics/*immunology, Flagellin/*genetics/*immunology/pharmacology, Gene Expression Regulation, Neoplastic/drug effects, Gene Knockout Techniques, HT29 Cells, Humans, Interleukin-8/genetics/metabolism, Intestinal Mucosa/immunology, NF-kappa B/genetics/metabolism, THP-1 Cells/immunology, Toll-Like Receptor 5/genetics/metabolism
  • topic2: Metabolism

Reference

@idauthorstitledoi/urlcatalogue
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
22238Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-100110Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 100110)
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
68380Automatically annotated from API rID32A
68381Automatically annotated from API rID32STR
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.MicrobeAtlas 1.0 betahttps://microbeatlas.org/
89423Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID397880.1