Strain identifier
BacDive ID: 130944
Type strain: 
Species: Enterococcus gallinarum
Strain Designation: JM-20
Strain history: <- T. Clavel, TU Munich, Freising-Weihenstephan, Germany; JM-20
NCBI tax ID(s): 1353 (species)
version 9.1 (current version)
General
@ref: 22236
BacDive-ID: 130944
DSM-Number: 28564
keywords: genome sequence, 16S sequence, Bacteria, microaerophile, human pathogen
description: Enterococcus gallinarum JM-20 is a microaerophile human pathogen that was isolated from caecal content; TNFdeltaARE/+ C57BL/6 mouse.
NCBI tax id
- NCBI tax id: 1353
- Matching level: species
strain history
- @ref: 22236
- history: <- T. Clavel, TU Munich, Freising-Weihenstephan, Germany; JM-20
doi: 10.13145/bacdive130944.20240916.9.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/bacillota
- domain: Bacteria
- phylum: Bacillota
- class: Bacilli
- order: Lactobacillales
- family: Enterococcaceae
- genus: Enterococcus
- species: Enterococcus gallinarum
- full scientific name: Enterococcus gallinarum (Bridge and Sneath 1982) Collins et al. 1984
synonyms
- @ref: 20215
- synonym: Streptococcus gallinarum
@ref: 22236
domain: Bacteria
phylum: Firmicutes
class: Bacilli
order: Lactobacillales
family: Enterococcaceae
genus: Enterococcus
species: Enterococcus gallinarum
full scientific name: Enterococcus gallinarum (Bridge and Sneath 1982) Collins et al. 1984
strain designation: JM-20
type strain: no
Morphology
colony morphology
- @ref: 22236
- incubation period: 1-2 days
Culture and growth conditions
culture medium
- @ref: 22236
- name: TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)
- growth: yes
- link: https://mediadive.dsmz.de/medium/92
- composition: Name: TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Yeast extract 3.0 g/l Distilled water
culture temp
- @ref: 22236
- growth: positive
- type: growth
- temperature: 37
Physiology and metabolism
oxygen tolerance
- @ref: 22236
- oxygen tolerance: microaerophile
metabolite utilization
| @ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
|---|---|---|---|---|
| 68381 | 29016 | arginine | + | hydrolysis |
| 68381 | 16988 | D-ribose | + | builds acid from |
| 68381 | 16899 | D-mannitol | + | builds acid from |
| 68381 | 30911 | sorbitol | - | builds acid from |
| 68381 | 17716 | lactose | + | builds acid from |
| 68381 | 27082 | trehalose | + | builds acid from |
| 68381 | 17992 | sucrose | + | builds acid from |
| 68381 | 30849 | L-arabinose | + | builds acid from |
| 68381 | 18333 | D-arabitol | - | builds acid from |
| 68381 | 40585 | alpha-cyclodextrin | - | builds acid from |
| 68381 | 606565 | hippurate | + | hydrolysis |
| 68381 | 28087 | glycogen | - | builds acid from |
| 68381 | 27941 | pullulan | - | builds acid from |
| 68381 | 17306 | maltose | + | builds acid from |
| 68381 | 6731 | melezitose | - | builds acid from |
| 68381 | 320055 | methyl beta-D-glucopyranoside | + | builds acid from |
| 68381 | 16443 | D-tagatose | + | builds acid from |
| 68381 | 16199 | urea | - | hydrolysis |
metabolite production
- @ref: 68381
- Chebi-ID: 15688
- metabolite: acetoin
- production: yes
metabolite tests
- @ref: 68381
- Chebi-ID: 15688
- metabolite: acetoin
- voges-proskauer-test: +
enzymes
| @ref | value | activity | ec |
|---|---|---|---|
| 68381 | urease | - | 3.5.1.5 |
| 68381 | glycyl tryptophan arylamidase | + | |
| 68381 | N-acetyl-beta-glucosaminidase | + | 3.2.1.52 |
| 68381 | pyrrolidonyl arylamidase | + | 3.4.19.3 |
| 68381 | beta-galactosidase | + | 3.2.1.23 |
| 68381 | Alanyl-Phenylalanyl-Proline arylamidase | - | |
| 68381 | alkaline phosphatase | - | 3.1.3.1 |
| 68381 | beta-glucuronidase | - | 3.2.1.31 |
| 68381 | beta-glucosidase | + | 3.2.1.21 |
| 68381 | arginine dihydrolase | + | 3.5.3.6 |
API rID32STR
| @ref | ADH Arg | beta GLU | beta GAR | beta GUR | alpha GAL | PAL | RIB | MAN | SOR | LAC | TRE | RAF | SAC | LARA | DARL | CDEX | VP | APPA | beta GAL | PyrA | beta NAG | GTA | HIP | GLYG | PUL | MAL | MEL | MLZ | Mbeta DG | TAG | beta MAN | URE |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 22236 | + | + | +/- | - | + | - | + | + | - | + | + | + | + | + | - | - | + | - | + | + | + | + | + | - | - | + | +/- | - | + | + | + | - |
| 22236 | + | + | - | - | - | - | + | + | - | + | + | - | + | + | - | - | + | - | + | + | + | + | + | - | - | + | - | - | + | + | - | - |
| 22236 | + | + | + | - | + | - | + | + | - | + | + | + | + | + | - | - | + | - | + | + | + | + | + | - | - | + | + | - | + | + | + | - |
| 22236 | + | + | +/- | - | + | - | + | + | - | + | + | + | + | + | - | - | + | - | + | + | + | + | + | - | - | + | +/- | - | + | + | + | - |
Isolation, sampling and environmental information
isolation
- @ref: 22236
- sample type: caecal content; TNFdeltaARE/+ C57BL/6 mouse
- geographic location: Freising
- country: Germany
- origin.country: DEU
- continent: Europe
isolation source categories
| Cat1 | Cat2 | Cat3 |
|---|---|---|
| #Host | #Mammals | #Muridae (Mouse/Rat) |
| #Host Body-Site | #Gastrointestinal tract | #Large intestine |
| #Host Body Product | #Gastrointestinal tract | #Caecal content |
taxonmaps
- @ref: 69479
- File name: preview.99_759.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_13;96_19;97_19;98_605;99_759&stattab=map
- Last taxonomy: Enterococcus
- 16S sequence: KR364766
- Sequence Identity:
- Total samples: 18861
- soil counts: 573
- aquatic counts: 1598
- animal counts: 15794
- plant counts: 896
Safety information
risk assessment
- @ref: 22236
- pathogenicity human: yes
- pathogenicity animal: yes
- biosafety level: 2
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 22236
- description: Enterococcus sp. JM20 16S ribosomal RNA gene, partial sequence
- accession: KR364766
- length: 1412
- database: nuccore
- NCBI tax ID: 1796630
Genome sequences
- @ref: 66792
- description: Enterococcus gallinarum DSM 28564
- accession: GCA_024622685
- assembly level: contig
- database: ncbi
- NCBI tax ID: 1353
Genome-based predictions
predictions
| @ref | trait | model | description | prediction | confidence | training_data |
|---|---|---|---|---|---|---|
| 69480 | gram-positive | gram-positive | Positive reaction to Gram-staining | yes | 84.713 | no |
| 69480 | anaerobic | anaerobic | Ability to grow under anoxygenic conditions (including facultative anaerobes) | no | 87.42 | no |
| 69480 | aerobic | aerobic | Ability to grow under oxygenic conditions (including facultative aerobes) | no | 93.613 | yes |
| 69480 | spore-forming | spore-forming | Ability to form endo- or exospores | no | 71.325 | no |
| 69480 | thermophilic | thermophile | Ability to grow at temperatures above or equal to 45°C | no | 93.921 | yes |
| 69480 | flagellated | motile2+ | Ability to perform flagellated movement | yes | 58.5 | no |
External links
@ref: 22236
culture collection no.: DSM 28564
straininfo link
- @ref: 89421
- straininfo: 398874
Reference
| @id | authors | title | doi/url | catalogue |
|---|---|---|---|---|
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | 10.1099/ijsem.0.004332 | |
| 22236 | Curators of the DSMZ | https://www.dsmz.de/collection/catalogue/details/culture/DSM-28564 | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 28564) | |
| 66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | https://diaspora-project.de/progress.html#genomes | |
| 68381 | Automatically annotated from API rID32STR | |||
| 69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | MicrobeAtlas 1.0 beta | https://microbeatlas.org/ | |
| 69480 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | https://diaspora-project.de/progress.html#genomes | |
| 89421 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | StrainInfo: A central database for resolving microbial strain identifiers | 10.60712/SI-ID398874.1 |