Strain identifier

BacDive ID: 130800

Type strain: Yes

Species: Prauserella coralliicola

Strain Designation: SP28S-3

Strain history: CGMCC 4.7182 <-- J.-M. Liu et al.; Inst. of Med. Biotechnol., Chin. Acad. of Med. Sci., China; SP28S-3.

NCBI tax ID(s): 1592324 (species)

« back to strain detail view
For citation purpose refer to the digital object identifier (doi) of the current version.
Archive
version 8.1 (current version):
version 8:
version 7.1:
version 7:
version 6:
version 5:
version 4.1:
version 4:
version 3:
version 2.1:
version 2:
version 1:
version 8.1 (current version)

General

@ref: 21859

BacDive-ID: 130800

DSM-Number: 46655

keywords: genome sequence, 16S sequence, Bacteria, spore-forming, mesophilic

description: Prauserella coralliicola SP28S-3 is a spore-forming, mesophilic bacterium that builds an aerial mycelium and was isolated from surface-sterilized healthy stem of Tamarix taklamakanensis.

NCBI tax id

  • NCBI tax id: 1592324
  • Matching level: species

strain history

@refhistory
21859<- C.-H. Sun, Chinese Acad. Med. Sciences & Peking Union Medical College,China; SP28S-3 <- J.-M. Liu
67770CGMCC 4.7182 <-- J.-M. Liu et al.; Inst. of Med. Biotechnol., Chin. Acad. of Med. Sci., China; SP28S-3.

doi: 10.13145/bacdive130800.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Pseudonocardiales
  • family: Pseudonocardiaceae
  • genus: Prauserella
  • species: Prauserella coralliicola
  • full scientific name: Prauserella coralliicola Wu et al. 2014

  • synonyms

    • @ref: 20215
    • synonym: Prauserella endophytica

@ref: 21859

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Pseudonocardiaceae

genus: Prauserella

species: Prauserella endophytica

full scientific name: Prauserella endophytica Liu et al. 2015

strain designation: SP28S-3

type strain: yes

Morphology

cell morphology

@refmotilityconfidencegram stain
69480no91.633
69480100positive

colony morphology

@refcolony colormedium used
69458ColourlessISP 2
69458ColourlessISP 6
69458Ivory (1014)suter with tyrosine
69458Ivory (1014)suter without tyrosine
69458Light ivory (1015)ISP 3
69458Light ivory (1015)ISP 4
69458Light ivory (1015)ISP 5
69458Light ivory (1015)ISP 7

multicellular morphology

@refforms multicellular complexcomplex namemedium namecomplex color
69458noAerial myceliumISP 2
69458yesAerial myceliumISP 3Ivory (1014)
69458noAerial myceliumISP 4
69458noAerial myceliumISP 5
69458noAerial myceliumISP 6
69458noAerial myceliumISP 7
69458noAerial myceliumsuter with tyrosine
69458noAerial myceliumsuter without tyrosine

pigmentation

@refproductionnamecolor
69458noMelanin
69458yessoluble pigmentSand yellow (1002)

multimedia

@refmultimedia contentcaptionintellectual property rights
21859https://www.dsmz.de/microorganisms/photos/DSM_46655.jpgMedium 535 28°C© Leibniz-Institut DSMZ
69458DSM_46655_image3.jpegPlates (65, ISP2, ISP3, ISP4, ISP5, ISP7)Helmholtz-Zentrum für Infektionsforschung GmbH
69458DSM_46655_image4.jpegPlates (65, ISP2, ISP3, ISP4, ISP5, ISP7)Helmholtz-Zentrum für Infektionsforschung GmbH

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
21859GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)yeshttps://mediadive.dsmz.de/medium/65Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water
21859TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535)yeshttps://mediadive.dsmz.de/medium/535Name: TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
21859positivegrowth28mesophilic
67770positivegrowth28mesophilic

Physiology and metabolism

spore formation

@refspore formationconfidence
69481yes99
69480yes100

halophily

  • @ref: 69458
  • salt: NaCl
  • growth: positive
  • tested relation: growth
  • concentration: 0-5 %

observation

  • @ref: 67770
  • observation: quinones: MK-9(H4), MK-8(H4), MK-10(H4)

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6945822599arabinose+/-growth
6945862968cellulose-growth
6945828757fructose+growth
6945817234glucose+growth
6945817268inositol+growth
6945837684mannose+growth
6945816634raffinose-growth
6945826546rhamnose+growth
6945817992sucrose+/-growth
6945818222xylose+growth
6837917632nitrate-reduction
683794853esculin-hydrolysis
6837916199urea-hydrolysis
683795291gelatin+hydrolysis
6837917634D-glucose-fermentation
6837916988D-ribose-fermentation
6837965327D-xylose-fermentation
6837916899D-mannitol-fermentation
6837917306maltose-fermentation
6837917716lactose-fermentation
6837917992sucrose-fermentation
6837928087glycogen-fermentation

enzymes

@refvalueactivityec
68379gelatinase+
68379urease-3.5.1.5
68379beta-glucosidase-3.2.1.21
68379N-acetyl-beta-glucosaminidase+3.2.1.52
68379alpha-glucosidase+3.2.1.20
68379beta-galactosidase-3.2.1.23
68379beta-glucuronidase-3.2.1.31
68379alkaline phosphatase+3.1.3.1
68379pyrrolidonyl arylamidase+3.4.19.3
68379pyrazinamidase-3.5.1.B15
68382alkaline phosphatase+3.1.3.1
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase+
68382trypsin-3.4.21.4
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase+3.2.1.20
68382N-acetyl-beta-glucosaminidase+3.2.1.52
68382alpha-mannosidase-3.2.1.24

API coryne

@refNITPYZPYRAPALbeta GURbeta GALalpha GLUbeta NAGESCUREGELGLURIBXYLMANMALLACSACGLYG
69458--++--++--+--------

API zym

@refAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
69458++/-+/--+++/--+/-++---++/-+-+/-

Isolation, sampling and environmental information

isolation

@refsample typehost speciesgeographic locationcountryorigin.countrycontinent
21859surface-sterilized healthy stem of Tamarix taklamakanensisTamarix taklamakanensisXinjiang, Taklarmakan desertChinaCHNAsia
67770Surface-sterilized stem of Tamarix taklamakanensis collected from the southern edge of Taklamakan desertTamarix taklamakanensisXinjiangChinaCHNAsia

isolation source categories

Cat1Cat2Cat3
#Host#Plants#Shrub (Scrub)
#Host#Plants#Tree
#Host Body-Site#Plant#Stem (Branch)
#Host Body-Site#Plant#Sterilized plant part

taxonmaps

  • @ref: 69479
  • File name: preview.99_64242.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_10;96_7302;97_8756;98_45421;99_64242&stattab=map
  • Last taxonomy: Prauserella
  • 16S sequence: KM670438
  • Sequence Identity:
  • Total samples: 326
  • soil counts: 205
  • aquatic counts: 10
  • animal counts: 91
  • plant counts: 20

Safety information

risk assessment

  • @ref: 21859
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 21859
  • description: Prauserella endophytica strain SP28S-3 16S ribosomal RNA gene, partial sequence
  • accession: KM670438
  • length: 1484
  • database: ena
  • NCBI tax ID: 1592324

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Prauserella endophytica strain CGMCC 4.71821592324.3wgspatric1592324
66792Prauserella endophytica CGMCC 4.71822894502394draftimg1592324
67770Prauserella endophytica CGMCC 4.7182GCA_005146945scaffoldncbi1592324

GC content

@refGC-contentmethod
2185969.7
6777069.7high performance liquid chromatography (HPLC)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingyes99no
motileno90.872no
gram-positiveyes91.049no
anaerobicno99.049no
aerobicyes94.035no
halophileno53.619no
spore-formingyes77.222no
thermophileno95.594yes
glucose-utilyes87.883no
flagellatedno96.644no
glucose-fermentno92.629yes

External links

@ref: 21859

culture collection no.: DSM 46655, CGMCC 4.7182, JCM 33043

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny25832628Prauserella endophytica sp. nov., an endophytic actinobacterium isolated from Tamarix taklamakanensis.Liu JM, Habden X, Guo L, Tuo L, Jiang ZK, Liu SW, Liu XF, Chen L, Li RF, Zhang YQ, Sun CHAntonie Van Leeuwenhoek10.1007/s10482-015-0435-12015Actinobacteria/*classification/genetics/*isolation & purification/physiology, Bacterial Typing Techniques, Base Composition, China, Cluster Analysis, Cytosol/chemistry, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Endophytes/*classification/genetics/*isolation & purification/physiology, Fatty Acids/analysis, Glycolipids/analysis, Molecular Sequence Data, Nucleic Acid Hybridization, Phospholipids/analysis, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Tamaricaceae/*microbiologyEnzymology
Phylogeny28857021Prauserella oleivorans sp. nov., a halophilic and thermotolerant crude-oil-degrading actinobacterium isolated from an oil-contaminated mud pit.Dastgheib SMM, Tirandaz H, Moshtaghi Nikou M, Ramezani M, Shavandi M, Amoozegar MA, Ventosa AInt J Syst Evol Microbiol10.1099/ijsem.0.0021242017Actinomycetales/*classification/genetics/isolation & purification, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Diaminopimelic Acid/chemistry, Fatty Acids/chemistry, Iran, Nucleic Acid Hybridization, Oil and Gas Fields/*microbiology, Peptidoglycan/chemistry, Petroleum/*microbiology, Petroleum Pollution, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Soil Microbiology, Soil Pollutants, Vitamin K 2/analogs & derivatives/chemistryTranscriptome
Phylogeny31671051Prauserella flavalba sp. nov., a novel species of the genus Prauserella, isolated from alkaline soil.Li Q, Ruckert C, Li G, Huang P, Schneider O, Kalinowski J, Jiang Y, Zotchev SB, Jiang CInt J Syst Evol Microbiol10.1099/ijsem.0.0037682020Actinobacteria/*classification/isolation & purification, Bacterial Typing Techniques, Base Composition, China, DNA, Bacterial/genetics, Fatty Acids/chemistry, Nucleic Acid Hybridization, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Soil Microbiology, Vitamin K 2/analogs & derivatives/chemistryTranscriptome

Reference

@idauthorstitledoi/urlcatalogue
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
21859Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-46655Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 46655)
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68379Automatically annotated from API Coryne
68382Automatically annotated from API zym
69458Wink, J.Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweighttps://cdn.dsmz.de/wink/DSM%2046655.pdf
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.MicrobeAtlas 1.0 betahttps://microbeatlas.org/
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. MünchPredictions based on the model GenomeNet Sporulation v. 110.21203/rs.3.rs-2527258/v1