Strain identifier
BacDive ID: 130731
Type strain: 
Species: Microbispora bryophytorum
Strain Designation: NEAU-TX2-2
Strain history: CGMCC 4.7138 <-- C. Liu; Northeast Agric. Univ., China; NEAU-TX2-2.
NCBI tax ID(s): 1460882 (species)
version 8.1 (current version)
General
@ref: 21669
BacDive-ID: 130731
DSM-Number: 46710
keywords: genome sequence, 16S sequence, Bacteria, spore-forming, mesophilic
description: Microbispora bryophytorum NEAU-TX2-2 is a spore-forming, mesophilic bacterium that was isolated from moss.
NCBI tax id
- NCBI tax id: 1460882
- Matching level: species
strain history
| @ref | history |
|---|---|
| 21669 | <- C. Liu, School of Life Science, Northeast Agricultural Univ., Harbin, China; NEAU-TX2-2 <- C. Li |
| 67770 | CGMCC 4.7138 <-- C. Liu; Northeast Agric. Univ., China; NEAU-TX2-2. |
doi: 10.13145/bacdive130731.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/actinomycetota
- domain: Bacteria
- phylum: Actinomycetota
- class: Actinomycetes
- order: Streptosporangiales
- family: Streptosporangiaceae
- genus: Microbispora
- species: Microbispora bryophytorum
- full scientific name: Microbispora bryophytorum Li et al. 2015
@ref: 21669
domain: Bacteria
phylum: Actinobacteria
class: Actinobacteria
order: Actinomycetales
family: Streptosporangiaceae
genus: Microbispora
species: Microbispora bryophytorum
full scientific name: Microbispora bryophytorum Li et al. 2015
strain designation: NEAU-TX2-2
type strain: yes
Morphology
cell morphology
| @ref | motility | confidence | gram stain |
|---|---|---|---|
| 69480 | no | 93.664 | |
| 69480 | 100 | positive |
multimedia
- @ref: 21669
- multimedia content: https://www.dsmz.de/microorganisms/photos/DSM_46710.jpg
- caption: Medium 65 28°C
- intellectual property rights: © Leibniz-Institut DSMZ
Culture and growth conditions
culture medium
| @ref | name | growth | link | composition |
|---|---|---|---|---|
| 21669 | GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) | yes | https://mediadive.dsmz.de/medium/65 | Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water |
| 21669 | ROLLED OATS MINERAL MEDIUM (DSMZ Medium 84) | yes | https://mediadive.dsmz.de/medium/84 | Name: ROLLED OATS MINERAL MEDIUM (DSMZ Medium 84) Composition: Agar 20.0 g/l Rolled oats 20.0 g/l ZnSO4 x 7 H2O 0.001 g/l MnCl2 x 4 H2O 0.001 g/l FeSO4 x 7 H2O 0.001 g/l Distilled water |
culture temp
| @ref | growth | type | temperature | range |
|---|---|---|---|---|
| 21669 | positive | growth | 28 | mesophilic |
| 67770 | positive | growth | 28 | mesophilic |
Physiology and metabolism
spore formation
| @ref | spore formation | confidence |
|---|---|---|
| 69481 | yes | 97 |
| 69480 | yes | 100 |
observation
- @ref: 67770
- observation: quinones: MK-9(H2), MK-9(H0), MK-9(H4)
Isolation, sampling and environmental information
isolation
| @ref | sample type | geographic location | country | origin.country | continent | latitude | longitude |
|---|---|---|---|---|---|---|---|
| 21669 | moss | Heilongjiang Province, Wuchang (44°15' N 127°54' E) | China | CHN | Asia | 44.25 | 127.9 |
| 67770 | Moss from Wuchang | Heilongjiang Province, north China | China | CHN | Asia |
isolation source categories
- Cat1: #Host
- Cat2: #Plants
- Cat3: #Moss
taxonmaps
- @ref: 69479
- File name: preview.99_29244.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_239;96_910;97_1060;98_1268;99_29244&stattab=map
- Last taxonomy: Microbispora
- 16S sequence: KF886293
- Sequence Identity:
- Total samples: 306
- soil counts: 241
- aquatic counts: 9
- animal counts: 13
- plant counts: 43
Safety information
risk assessment
- @ref: 21669
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 21669
- description: Microbispora bryophytorum strain NEAU-TX2-2 16S ribosomal RNA gene, partial sequence
- accession: KF886293
- length: 1513
- database: ena
- NCBI tax ID: 1460882
Genome sequences
| @ref | description | accession | assembly level | database | NCBI tax ID |
|---|---|---|---|---|---|
| 66792 | Microbispora bryophytorum CGMCC 4.7138 | GCA_014645855 | scaffold | ncbi | 1460882 |
| 66792 | Microbispora bryophytorum NEAU-TX2-2 | GCA_014712735 | contig | ncbi | 1460882 |
| 66792 | Microbispora bryophytorum strain CGMCC 4.7138 | 1460882.4 | wgs | patric | 1460882 |
| 66792 | Microbispora bryophytorum strain DSM 46710 | 1460882.3 | wgs | patric | 1460882 |
| 66792 | Microbispora bryophytorum DSM 46710 | 2891475856 | draft | img | 1460882 |
| 67770 | Microbispora bryophytorum DSM 46710 | GCA_006874465 | scaffold | ncbi | 1460882 |
GC content
| @ref | GC-content | method |
|---|---|---|
| 21669 | 70.4 | thermal denaturation, midpoint method (Tm) |
| 67770 | 70.2-70.6 | thermal denaturation, midpoint method (Tm) |
Genome-based predictions
predictions
| trait | prediction | confidence | training_data |
|---|---|---|---|
| spore-forming | yes | 97 | no |
| flagellated | no | 98.164 | no |
| gram-positive | yes | 91.413 | no |
| anaerobic | no | 99.19 | no |
| aerobic | yes | 92.662 | no |
| halophile | no | 93.037 | no |
| spore-forming | yes | 95.166 | no |
| glucose-util | yes | 90.002 | no |
| thermophile | no | 98.122 | yes |
| motile | no | 93.387 | no |
| glucose-ferment | no | 93.324 | no |
External links
@ref: 21669
culture collection no.: DSM 46710, CGMCC 4.7138, JCM 31772
straininfo link
- @ref: 89234
- straininfo: 404685
literature
| topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
|---|---|---|---|---|---|---|---|---|
| Phylogeny | 25634944 | Microbispora bryophytorum sp. nov., an actinomycete isolated from moss (Bryophyta). | Li C, Zhang Y, Liu C, Wang H, Zhao J, Li L, Zhang Z, Wang X, Xiang W | Int J Syst Evol Microbiol | 10.1099/ijs.0.000095 | 2015 | Actinobacteria/*classification/genetics/isolation & purification, Bacterial Typing Techniques, Bryophyta/*microbiology, China, DNA, Bacterial/genetics, Diaminopimelic Acid/chemistry, Fatty Acids/chemistry, Molecular Sequence Data, Nucleic Acid Hybridization, Peptidoglycan/chemistry, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Spiro Compounds, Vitamin K 2/analogs & derivatives/chemistry | Genetics |
| Phylogeny | 26589683 | Microbispora camponoti sp. nov., a novel actinomycete isolated from the cuticle of Camponotus japonicus Mayr. | Han C, Liu C, Zhao J, Guo L, Lu C, Li J, Jia F, Wang X, Xiang W | Antonie Van Leeuwenhoek | 10.1007/s10482-015-0625-x | 2015 | Actinomycetales/classification/genetics/*isolation & purification/metabolism, Animal Structures/*microbiology, Animals, Ants/*microbiology, China, DNA, Bacterial/genetics, DNA, Ribosomal/genetics, Fatty Acids/chemistry/metabolism, Molecular Sequence Data, Phylogeny | Metabolism |
| Phylogeny | 30247118 | Microbispora triticiradicis sp. nov., a novel actinomycete isolated from the root of wheat (Triticum aestivum L.). | Han C, Tian Y, Zhao J, Yu Z, Jiang S, Guo X, Xiang W, Wang X | Int J Syst Evol Microbiol | 10.1099/ijsem.0.003040 | 2018 | Actinomycetales/*classification/genetics/isolation & purification, Bacterial Typing Techniques, Base Composition, China, DNA, Bacterial/genetics, Fatty Acids/chemistry, Nucleic Acid Hybridization, Phospholipids/chemistry, *Phylogeny, Plant Roots/*microbiology, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Triticum/*microbiology, Vitamin K 2/analogs & derivatives/chemistry | Transcriptome |
| Phylogeny | 30739286 | Microbispora tritici sp. nov., a novel actinomycete isolated from a root of wheat (Triticum aestivum L.). | Han C, Zhao J, Yu B, Shi H, Zhang C, Guo X, Xiang W, Wang X | Antonie Van Leeuwenhoek | 10.1007/s10482-019-01246-y | 2019 | Actinobacteria/*classification/genetics/*isolation & purification/physiology, Bacterial Typing Techniques, Cell Wall/chemistry, Cluster Analysis, Cytosol/chemistry, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Diaminopimelic Acid/analysis, Fatty Acids/analysis, Glycolipids/analysis, Nucleic Acid Hybridization, Phospholipids/analysis, Phylogeny, Plant Roots/*microbiology, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Triticum/*microbiology, Vitamin K 2/analysis | Transcriptome |
| Phylogeny | 31622227 | Microbispora fusca sp. nov., a novel actinomycete isolated from the ear of wheat (Triticum aestivum L.). | Zhao J, Yu B, Han C, Cao P, Yu Z, Ju H, Xiang W, Wang X | Int J Syst Evol Microbiol | 10.1099/ijsem.0.003725 | 2020 | Actinomycetales/*classification/isolation & purification, Bacterial Typing Techniques, Base Composition, China, DNA, Bacterial/genetics, Fatty Acids/chemistry, Genes, Bacterial, Nucleic Acid Hybridization, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Triticum/*microbiology | Transcriptome |
| Phylogeny | 33047275 | Microbispora cellulosiformans sp. nov., a novel actinomycete with cellulase activity isolated from soil in the cold region. | Gong X, Xiang W, Cao X, Yu Y, Hao Y, Li L, Wang Q, Zou H, Qian C | Antonie Van Leeuwenhoek | 10.1007/s10482-020-01477-4 | 2020 | *Actinobacteria/genetics, Bacterial Typing Techniques, *Cellulases, DNA, Bacterial/genetics, Fatty Acids, Nucleic Acid Hybridization, Phospholipids, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Soil, Soil Microbiology | Transcriptome |
| Phylogeny | 33095132 | Microbispora clausenae sp. nov., an endophytic actinobacterium isolated from the surface-sterilized stem of a Thai medicinal plant, Clausena excavala Burm. f. | Kaewkla O, Koomsiri W, Thamchaipenet A, Franco CMM | Int J Syst Evol Microbiol | 10.1099/ijsem.0.004518 | 2020 | Actinobacteria/*classification/isolation & purification, Bacterial Typing Techniques, Base Composition, Clausena/*microbiology, DNA, Bacterial/genetics, Fatty Acids/chemistry, Nucleic Acid Hybridization, Phospholipids/chemistry, *Phylogeny, Plant Stems/microbiology, Plants, Medicinal/microbiology, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Thailand, Vitamin K 2/analogs & derivatives/chemistry | Transcriptome |
| Phylogeny | 33528352 | Microbispora sitophila sp. nov., a novel actinobacterium isolated from rhizosphere soil of wheat (Triticum aestivum L.). | Guo L, Li Z, Xu X, Pang Q, Zhao J | Int J Syst Evol Microbiol | 10.1099/ijsem.0.004684 | 2021 |
Reference
| @id | authors | title | doi/url | catalogue |
|---|---|---|---|---|
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | 10.1099/ijsem.0.004332 | |
| 21669 | Curators of the DSMZ | https://www.dsmz.de/collection/catalogue/details/culture/DSM-46710 | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 46710) | |
| 66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | https://diaspora-project.de/progress.html#genomes | |
| 67770 | Curators of the JCM | https://jcm.brc.riken.jp/en/ | ||
| 69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | MicrobeAtlas 1.0 beta | https://microbeatlas.org/ | |
| 69480 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | https://diaspora-project.de/progress.html#genomes | |
| 69481 | Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch | Predictions based on the model GenomeNet Sporulation v. 1 | 10.21203/rs.3.rs-2527258/v1 | |
| 89234 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | StrainInfo: A central database for resolving microbial strain identifiers | 10.60712/SI-ID404685.1 |