Strain identifier

BacDive ID: 130527

Type strain: Yes

Species: Mesorhizobium robiniae

Strain history: <- HAMBI <- G. H. Wei, College of Life Sciences, Shaanxi, China; CCNWYC 115

NCBI tax ID(s): 559315 (species)

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General

@ref: 22035

BacDive-ID: 130527

DSM-Number: 100022

keywords: genome sequence, 16S sequence, Bacteria, aerobe, mesophilic, Gram-negative, motile, rod-shaped

description: Mesorhizobium robiniae DSM 100022 is an aerobe, mesophilic, Gram-negative bacterium that was isolated from root nodule of Robinia pseudoacacia.

NCBI tax id

  • NCBI tax id: 559315
  • Matching level: species

strain history

  • @ref: 22035
  • history: <- HAMBI <- G. H. Wei, College of Life Sciences, Shaanxi, China; CCNWYC 115

doi: 10.13145/bacdive130527.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Alphaproteobacteria
  • order: Hyphomicrobiales
  • family: Phyllobacteriaceae
  • genus: Mesorhizobium
  • species: Mesorhizobium robiniae
  • full scientific name: Mesorhizobium robiniae Zhou et al. 2010

@ref: 22035

domain: Bacteria

phylum: Proteobacteria

class: Alphaproteobacteria

order: Rhizobiales

family: Phyllobacteriaceae

genus: Mesorhizobium

species: Mesorhizobium robiniae

full scientific name: Mesorhizobium robiniae Zhou et al. 2010

type strain: yes

Morphology

cell morphology

  • @ref: 29713
  • gram stain: negative
  • cell length: 1-2.5 µm
  • cell width: 0.3-0.6 µm
  • cell shape: rod-shaped
  • motility: yes

colony morphology

  • @ref: 22035
  • incubation period: 2-3 days

pigmentation

  • @ref: 29713
  • production: yes

Culture and growth conditions

culture medium

  • @ref: 22035
  • name: RHIZOBIUM MEDIUM (DSMZ Medium 98)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/98
  • composition: Name: RHIZOBIUM MEDIUM (DSMZ Medium 98) Composition: air-dried garden soil 80.0 g/l Agar 15.0 g/l Mannitol 10.0 g/l Yeast extract 1.0 g/l Na2CO3 0.2 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
22035positivegrowth30mesophilic
29713positivegrowth15-40
29713positiveoptimum25-30mesophilic

culture pH

@refabilitytypepHPH range
29713positivegrowth06-11alkaliphile
29713positiveoptimum06-08

Physiology and metabolism

oxygen tolerance

  • @ref: 29713
  • oxygen tolerance: aerobe

spore formation

  • @ref: 29713
  • spore formation: no

halophily

@refsaltgrowthtested relationconcentration
29713NaClpositivegrowth5 %
29713NaClpositiveoptimum5 %

observation

  • @ref: 29713
  • observation: aggregates in chains

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
2971322599arabinose+carbon source
2971328757fructose+carbon source
2971317234glucose+carbon source
2971317716lactose+carbon source
2971317306maltose+carbon source
2971337684mannose+carbon source
2971316634raffinose+carbon source
2971333942ribose+carbon source
2971330911sorbitol+carbon source
2971330031succinate+carbon source
2971318222xylose+carbon source
6836925115malate-assimilation
6836917128adipate-assimilation
6836927689decanoate-assimilation
6836924265gluconate-assimilation
6836917306maltose-assimilation
6836959640N-acetylglucosamine-assimilation
6836916899D-mannitol-assimilation
6836916024D-mannose-assimilation
6836930849L-arabinose-assimilation
6836917634D-glucose-assimilation
683695291gelatin-hydrolysis
683694853esculin-hydrolysis
6836916199urea-hydrolysis
6836929016arginine-hydrolysis
6836917634D-glucose-fermentation
6836927897tryptophan-energy source
6836917632nitrate-reduction

metabolite production

  • @ref: 68369
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

  • @ref: 68369
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: -

enzymes

@refvalueactivityec
22035catalase+1.11.1.6
22035cytochrome-c oxidase+1.9.3.1
68369gelatinase-
68369beta-glucosidase-3.2.1.21
68369urease-3.5.1.5
68369arginine dihydrolase-3.5.3.6

API 20NE

@refNO3TRPGLU_ FermADH ArgUREESCGELPNPGGLU_ AssimARAMNEMANNAGMALGNTCAPADIMLTCITPAC
22035--------------------

Isolation, sampling and environmental information

isolation

  • @ref: 22035
  • sample type: root nodule of Robinia pseudoacacia
  • host species: Robinia pseudoacacia
  • geographic location: Yangling, Shaanxi Province
  • country: China
  • origin.country: CHN
  • continent: Asia

isolation source categories

Cat1Cat2Cat3
#Host#Plants#Tree
#Host Body-Site#Plant#Root nodule

taxonmaps

  • @ref: 69479
  • File name: preview.99_1684.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_5;96_220;97_239;98_268;99_1684&stattab=map
  • Last taxonomy: Mesorhizobium
  • 16S sequence: EU849582
  • Sequence Identity:
  • Total samples: 4459
  • soil counts: 2635
  • aquatic counts: 507
  • animal counts: 282
  • plant counts: 1035

Safety information

risk assessment

  • @ref: 22035
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 22035
  • description: Mesorhizobium robiniae strain CCNWYC 115 16S ribosomal RNA gene, partial sequence
  • accession: EU849582
  • length: 1356
  • database: ena
  • NCBI tax ID: 559315

Genome sequences

  • @ref: 66792
  • description: Mesorhizobium robiniae DSM 100022
  • accession: 2928416989
  • assembly level: draft
  • database: img
  • NCBI tax ID: 559315

GC content

  • @ref: 29713
  • GC-content: 61.5

External links

@ref: 22035

culture collection no.: DSM 100022, ACCC 14543, HAMBI 3082, CCNWYC 115

straininfo link

  • @ref: 89050
  • straininfo: 402089

literature

  • topic: Phylogeny
  • Pubmed-ID: 20008111
  • title: Mesorhizobium robiniae sp. nov., isolated from root nodules of Robinia pseudoacacia.
  • authors: Zhou PF, Chen WM, Wei GH
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijs.0.019356-0
  • year: 2009
  • mesh: Alphaproteobacteria/*classification/genetics/*isolation & purification/metabolism, Bacterial Proteins/genetics/metabolism, DNA, Bacterial/genetics, Molecular Sequence Data, Phylogeny, RNA, Ribosomal, 16S/genetics, Robinia/*microbiology, Root Nodules, Plant/*microbiology
  • topic2: Metabolism

Reference

@idauthorstitledoi/urlcatalogueID_cross_referencepubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
22035Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-100022Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 100022)
29713Barberan A, Caceres Velazquez H, Jones S, Fierer N.Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information10.1128/mSphere.00237-172609628776041
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
68369Automatically annotated from API 20NE
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.MicrobeAtlas 1.0 betahttps://microbeatlas.org/
89050Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID402089.1